1-3: ᝈ⪅᳨య
astA (88bp) 100bp
500bp
(B) Semi-nested PCR ( astA )
M N P 1 2 3 4 5
M: Ladder maker N: 㝜ᛶࢥࣥࢺ࣮ࣟࣝ
P: 㝧ᛶࢥࣥࢺ࣮ࣟࣝ
1-5: ᝈ⪅᳨య
78
ᅗ 㸫ᇵ㣴㝧ᛶ᳨యཬ䜃ᇵ㣴㝜ᛶ᳨య䛻䛚䛡䜛 㼏㼖㻜㻠㻝㻠㻘㻌㼑㼍㼑㻘㻌㼍㼟㼠㻭 䛾 㼏㼥㼏㼘㼑㻌 㼠㼔㼞㼑㼟㼔㼛㼘㼐㻌 ್䠄㻯㼠
್䠅䚹ᩓᕸᅗ䛿 㻾㻲㻮㻿㻞㻠㻌 㼢㼑㼞㻚㻡 䛾 㻯㼠 ್䛾୰ኸ್䠈㻥㻡㻑ཬ䜃 㻥㻥㻑ಙ㢗༊㛫䜢♧䛧䛯䚹㻟 ✀㢮䛾ᶆⓗ㑇 ఏᏊ䛾 㻯㼠 ୰ኸ್䛻䛴䛔䛶䠈ᇵ㣴㝧ᛶ᳨య䛸ᇵ㣴㝜ᛶ᳨య㛫䛷᭷ពᕪ䛜ㄆ䜑䜙䜜䛯㻌࣓ࢹ
᳨ࣥᐃS㸺S㸺䚹
5)%6YHU5DSLGIRRGERUQHEDFWHULDOVFUHHQLQJYHU
㻥㻥㻑ಙ㢗༊㛫ୖ㝈್ཪ䛿ୗ㝈್㻌 㻥㻡㻑ಙ㢗༊㛫ୖ㝈್ཪ䛿ୗ㝈್㻌
୰ኸ್㻌
㸨 㸨㸨 㸨㸨
ᇵ㣴㻌 㝧ᛶ㻌
ᇵ㣴㻌 㝜ᛶ㻌
ᇵ㣴㻌 㝧ᛶ㻌
ᇵ㣴㻌
㝜ᛶ㻌 ᇵ㣴㻌
㝧ᛶ㻌
ᇵ㣴㻌 㝜ᛶ㻌
㻯㻚㻌㼖㼑㼖㼡㼚㼕㻌㻔㼏㼖㻜㻠㻝㻠㻕㻌 㼑㼍㼑㝧ᛶ⭠⳦㻌 㼍㼟㼠㻭㝧ᛶ⭠⳦㻌
⥲ ᣓ 㻌 㻌
ᅜ ෆ 䛷䛿䠈ᖹ ᡂ 㻝㻥 ᖺ 䛛䜙ᖹ ᡂ 㻞㻣 ᖺ 䛾ᐇ ⦼ 䛷䠈ᖺ 㛫 㻥㻟㻝䡚㻝㻟㻢㻥 ௳ 䛾㣗 ୰ ẘ
䛜Ⓨ ⏕ 䛧䛶䛔䜛䚹㣗 ୰ ẘ Ⓨ ⏕ 䛻䛿䠈ಖ ᡤ 䛿 ḟ ⿕ ᐖ 䛾ᣑ 䜢㜵 Ṇ 䛩䜛䛯䜑䠈
㏿ 䜔䛛䛺ཎ ᅉ ✲ ᫂ 䜢 ồ 䜑䜙䜜䜛 䚹ᆅ ᪉ ⾨ ⏕ ◊ ✲ ᡤ ➼ 䛾ヨ 㦂 ◊ ✲ ᶵ 㛵 䛿ཎ ᅉ ✲ ᫂ 䛾䛯䜑䛾ᇵ 㣴 ἲ 䜢⾜ 䛖䛜䠈⤖ ᯝ ุ ᐃ 䜎䛷䛻᭱ 䛷 㻣 ᪥ ௨ ୖ せ 䛩䜛䚹䛣䛾ㄢ 㢟 䜢ゎ Ỵ 䛩䜛䛯䜑䠈⚟ ᓥ 䜙䛿䠈ᝈ ⪅ ⣅ ౽ 䛛䜙 㻞㻠 ✀ 㢮 䛾㣗 ୰ ẘ ⳦ ᶆ ⓗ 㑇 ఏ Ꮚ 䜢 㻟 㛫 ௨ ෆ 䛻᳨ ฟ 䛩䜛䝸䜰䝹䝍䜲䝮 㻼㻯㻾 ἲ 䜢㛤 Ⓨ 䛧䠈㻾㻲㻮㻿㻞㻠 䛸 ⛠ 䛧䛯䚹䛧䛛䛧䠈㻿㼍㼘㼙㼛㼚㼑㼘㼘㼍㻌 㼟㼜㼜㻚䛺䛹䛾」 ᩘ 䛾⳦ ✀ 䛷᳨ ฟ ឤ ᗘ 䛜ᝏ 䛟䠈㣗 ୰ ẘ 䛾ᚤ ⏕ ≀ ヨ 㦂 䛻ᛂ ⏝ 䛩䜛䛯 䜑䛻䛿ᨵ Ⰻ 䛜ᚲ せ 䛷䛒䛳䛯䚹ᮏ ◊ ✲ 䛷䛿䠈㻾㻲㻮㻿㻞㻠 䛾ឤ ᗘ 䜢ᨵ ၿ 䛩䜛䛯䜑䛻䠈᰾ 㓟
⢭ 〇 ἲ 䛾᭱ 㐺 䠈㻾㻲㻮㻿㻞㻠 䛾䝥䝷䜲䝬䞊䜔 㻵㻭㻯 䛾ᨵ Ⰻ 䠈ᝈ ⪅ ⣅ ౽ ᳨ య 䜢⏝ 䛔䛯ホ ౯ 䜢⾜ 䛔䠈௨ ୗ 䛾ᡂ ᯝ 䜢ᚓ 䛯䚹㻌
➨ ୍ ❶ 䛷 䛿䠈 ⣅ ౽ 䛻ྵ 䜎䜜䜛䜾䝷䝮㝜 ᛶ ⳦ ཬ 䜃䜾䝷䝮㝧 ᛶ ⳦ 䛾 ᪉ 䜢ຠ ᯝ ⓗ 䛻 ᢳ ฟ 䛩䜛䛯䜑䠈᰾ 㓟 ⢭ 〇 ἲ 䛾ẚ ㍑ ᳨ ウ 䜢⾜ 䛳䛯 㻾㻲㻮㻿㻞㻠 䛸ే ⏝ 䛧䛶䛔䜛᰾ 㓟 ᢳ ฟ 䜻䝑䝖䠄㻽㼗㼕㼠䠅䛿䠈⏺ 㠃 ά ᛶ 䛷⳦ య 䜢⁐ ゎ 䛧䛶䛔䜛䛜䠈 䜾䝷䝮㝧 ᛶ ⳦ 䛾᰾ 㓟 ᢳ ฟ ຠ
⋡ 䛜 ప 䛔 䛣 䛸 䛜 ᠱ ᛕ 䛥 䜜 䠈 ヨ 㦂 ᡂ ⦼ 䛻 ᙳ 㡪 䜢 䛘 䜛 ྍ ⬟ ᛶ 䛜 䛒 䛳 䛯 䚹 䛭 䛣 䛷 䠈㻯㻚㻌 㼜㼑㼞㼒㼞㼕㼚㼓㼑㼚㼟㻘㻌㻿㻚㻌㼍㼡㼞㼑㼡㼟㻘㻌㻿㻚㻌㼀㼥㼜㼔㼕㼙㼡㼞㼕㼡㼙㻘㻌㻯㻚㻌㼖㼑㼖㼡㼚㼕 䜢ྵ 䜐ᶍ ᨃ ⣅ ౽ ᳨ య 䛛䜙䠈䝡䞊 䝈䛻䜘䛳䛶≀ ⌮ ⓗ 䛻⳦ య 䜢◚ ○ 䛩䜛ᢳ ฟ ἲ 䠄㼁㼗㼕㼠䠅䛸 㻽㼗㼕㼠 䜢⏝ 䛔䛶᰾ 㓟 䜢ᢳ ฟ 䛧䠈 㻿㻳㻙㻼㻯㻾 ἲ ཬ 䜃 㼝㻙㻼㻯㻾 ἲ 䛷 ᚓ 䜙䜜 䛯ᡂ ⦼ 䛛䜙䠈᰾ 㓟 ᢳ ฟ ຠ ⋡ 䜢 ẚ ㍑ 䛧䛯䚹 㻿㻳㻙 㻼㻯㻾 ἲ 䛻䛴䛔䛶䛿䠈㼁㼗㼕㼠 䛾᰾ 㓟 ᢳ ฟ ຠ ⋡ 䛜୍ ␒ 㧗 䛟䠈◚ ○ ⨨ 䛿 㻲㼍㼟㼠㼜㼞㼑㼜㻞㻠 䛾
⏝ 䛜᭷ ⏝ 䛷䛒䛳䛯䚹㻾㻲㻮㻿㻞㻠 䛻䜘䜛 㻯㻚㻌 㼜㼑㼞㼒㼞㼕㼚㼓㼑㼚㼟㻘㻌㻿㻚㻌 㼍㼡㼞㼑㼡㼟㻘㻌 㻿㻚㻌㼀㼥㼜㼔㼕㼙㼡㼞㼕㼡㼙㻘㻌 㻯㻚㻌 㼖㼑㼖㼡㼚㼕䛩䜉䛶䛾᳨ ฟ ឤ ᗘ 䜢ྥ ୖ 䛥䛫䜛 㻲㼍㼟㼠㼜㼞㼑㼜㻌 㻞㻠 䛾◚ ○ ᮲ ௳ 䛸䛧䛶䛿 㻠㻚㻜㼙㻛㼟㻌 㻢㻜 ⛊ 䛜㐺 ษ 䛷䛒䛳䛯䚹㼝㻙㻼㻯㻾 ἲ 䛻䜘䜛ᶆ ⓗ 㑇 ఏ Ꮚ 䝁䝢䞊ᩘ 䛻䛴䛔䛶䜒䠈㼁㼗㼕㼠 ฎ ⌮
⩌ 䛾⢭ 〇 㻰㻺㻭 䛿䠈㻽㼗㼕㼠 ฎ ⌮ ⩌ 䛸ẚ ㍑ 䛩䜛䛸䠈㻤䡚㻞㻟㻠 ಸ ከ 䛟䠈㻽㼗㼕㼠 䛸 㼁㼗㼕㼠 䛾᰾ 㓟
80
ᢳ ฟ ຠ ⋡ 䛾 ᕪ ␗ 䜢 ᐃ 㔞 ⓗ 䛻☜ ㄆ 䛧 䛯 䚹 ᝈ ⪅ ⣅ ౽ ᳨ య 䜢 ⏝ 䛔䛯 ホ ౯ 䛷 䜒 䠈 㻾㻲㻮㻿㻞㻠 䛸 㼁㼗㼕㼠 䛾ే ⏝ 䛻䜘䜚᳨ ฟ ⋡ 䛜ྥ ୖ 䛧䠈㻾㻲㻮㻿㻞㻠 ཬ 䜃 㻿㻳㻙㻼㻯㻾 ἲ 䠈୪ 䜃䛻 㼝㻙㻼㻯㻾 ἲ 䛾᳨ ฟ ឤ ᗘ 䛾ྥ ୖ 䛻 㼁㼗㼕㼠 䛾ే ⏝ 䛿᭷ ຠ 䛺᪉ ἲ 䛷䛒䜛䛣䛸䛜♧ 䛥䜜䛯䚹 㻌
➨ ❶ 䛷䛿䠈㻾㻲㻮㻿㻞㻠 䛾᳨ ฟ ឤ ᗘ 䜢ྥ ୖ 䛥䛫䜛䛯䜑䠈䝥䝷䜲䝬䞊䛸 㻵㻭㻯 䜢ᨵ Ⰻ 䛧 䛯 䚹 䝥 䝷 䜲 䝬 䞊 䝎 䜲 䝬 䞊 䛾 Ⓨ ⏕ ᢚ Ṇ 䛸 䝥 䝷 䜲 䝬 䞊 㛫 䛾 ᖸ ΅ స ⏝ 䜢 ⦆ 䛩 䜛 䛯 䜑 䠈 㻾㻲㻮㻿㻞㻠 䛷⏝ 䛔䜙䜜䜛䝥䝷䜲䝬䞊㻝㻟 ⤌ 䜢 タ ィ 䛧䠈䝥䝷䜲䝬䞊䛾⤌ 䜏ྜ 䜟䛫䜢ぢ ┤ 䛧 䛯䚹䛥䜙䛻䠈㻾㻲㻮㻿㻞㻠 䛾 せ 㣗 ୰ ẘ ⳦ 㻤 ⳦ ✀ 䜢ᶆ ⓗ 䛸䛩䜛䝥䝷䜲䝬䞊䜢㐃 ⤖ 䛧䛯➇ ྜ ᆺ 㻵㻭㻯 䜢タ ィ 䛩䜛䛣䛸䛷䠈㻾㻲㻮㻿㻞㻠㻌 㼢㼑㼞㻚㻡 䜢㛤 Ⓨ 䛧䛯䚹㻾㻲㻮㻿㻞㻠㼢㼑㼞㻚㻡 䛾≉ ␗ ᛶ 䛾ホ ౯ 䜢 ⾜ 䛳 䛯䛸䛣䜝䠈ᶆ ⓗ 䛸䛩䜛㣗 ୰ ẘ ⳦ 䜢䛩䜉䛶᳨ ฟ 䛧䠈㠀 ≉ ␗ ⓗ 䛺 ᛂ 䛿ㄆ 䜑 䜙 䜜䛺䛛䛳䛯䚹᳨ ฟ 㝈 ⏺ 䜢ホ ౯ 䛧䛯䛸䛣䜝䠈㻾㻲㻮㻿㻞㻠 䛸ẚ ㍑ 䛩䜛䛸䠈㻝㻜䡚㻝㻜㻜 ಸ 䛾ᨵ ၿ 䜢☜ ㄆ 䛧䛯䚹䛥䜙䛻䠈㻾㻲㻮㻿㻞㻠㻌 㼢㼑㼞㻚㻡 䛿 㻱㻴㻱㻯 䛾 㼟㼠㼤㻞 䝃䝤䝍䜲䝥䛷䛒䜛㼟㼠㼤㻞㼍㻘㻌 㼟㼠㼤㻞㼎㻘㻌 㼟㼠㼤㻞㼏㻘㻌 㼟㼠㼤㻞㼐㻘㻌 㼟㼠㼤㻞㼑㻘㻌 㼟㼠㼤㻞㼒㻘㻌 㼟㼠㼤㻞㼓䠈୪ 䜃䛻 㻾㻲㻮㻿㻞㻠 䛷᳨ ฟ 䛷䛝䛺䛛䛳䛯 㻿㼀㼔 㑇 ఏ Ꮚ ཬ 䜃 㼠㼞㼔㻞 䜒᳨ ฟ ྍ ⬟ 䛸䛺䛳䛯䚹Ᏻ ౯ 䛻䛩䜉䛶䛾 㼟㼠㼤㻞 䝃䝤䝍䜲䝥䜢᳨ ฟ 䛷䛝䜛᪉ ἲ 䛸䛧䛶䠈 ᚋ 䛾ᐇ ⏝ 䛜ᮇ ᚅ 䛥䜜䜛䚹㻱㻴㻱㻯㻘㻌㻯㼍㼙㼜㼥㼘㼛㼎㼍㼏㼞㼑㼞㻘㻌㻿㼍㼘㼙㼛㼚㼑㼘㼘㼍㻌㼟㼜㼜㻚䛻 䜘 䜛 ᝈ ⪅ ⣅ ౽ ᳨ య 䜢 ⏝ 䛔 䛯 ホ ౯ 䛷 䛿 䠈 㻾㻲㻮㻿㻞㻠㼢㼑㼞㻚㻡 䛸 㼁㼗㼕㼠 䛾 ే ⏝ 䛻 䜘 䛳 䛶 䠈 㻾㻲㻮㻿㻞㻠 䜘䜚᳨ ฟ ឤ ᗘ 䛜ྥ ୖ 䛧䠈㻱㻴㻱㻯 䛸 㻯㼍㼙㼜㼥㼘㼛㼎㼍㼏㼠㼑㼞㻌 㼟㼜㼜㻚䛻䛴䛔䛶䛿䠈ᇵ 㣴 ἲ 䛸ྠ ឤ ᗘ 䠈ཪ 䛿㧗 䛔᳨ ฟ ឤ ᗘ 䜢 ᚓ 䜛䛣䛸䛜䛷䛝䛯䠄ឤ ᗘ 䠖 㻜㻚㻤㻥䡚㻝㻚㻜㻜䠅䚹᳨ ฟ ឤ ᗘ 䛾
ྥ ୖ 䛻䜘䜚୍ 㒊 䛾⳦ ✀ 䛷ᇵ 㣴 ⤖ ᯝ 䛸䛾 ୍ ⮴ 䛜ㄆ 䜑䜙䜜䛯䛜䚸 㻾㻲㻮㻿㻞㻠㻌 㼢㼑㼞㻚㻡 䛿䠈 㻱㻴㻱㻯㻘㻌㻯㻚㻌 㼖㼑㼖㼡㼚㼕㻘㻌㻿㼍㼘㼙㼛㼚㼑㼘㼘㼍㻌㼟㼜㼜㻚䛻䜘䜛㣗 ୰ ẘ Ⓨ ⏕ 䛾ᚤ ⏕ ≀ ヨ 㦂 䛸䛧䛶䠈᭷ ⏝ 䛷䛒䜛䛣䛸䛜♧ 䛥䜜䛯䚹㻌
➨ ୕ ❶ 䛷䛿䠈⫶ ⭠ ⅖ ᝈ ⪅ 䛾⣅ ౽ 䛸ྤ ≀ 㻞㻠㻢 ᳨ య 䛻䛴䛔䛶䠈㻾㻲㻮㻿㻞㻠㻌㼢㼑㼞㻚㻡 䛻䜘䜛 ゎ ᯒ 䜢⾜ 䛔䠈᳨ ᰝ ⢭ ᗘ 䛾ᨵ ၿ 䜢ヨ 䜏䛯䚹㻾㻲㻮㻿㻞㻠㻌 㼢㼑㼞㻚㻡 䛻䜘䜛㻯㻚㻌 㼖㼑㼖㼡㼚㼕㻌䠄㼏㼖㻜㻠㻝㻠䠅㻘㻌 㼑㼍㼑 㝧 ᛶ ⭠ ⳦ 䠈㼍㼟㼠㻭 㝧 ᛶ ⭠ ⳦ 䛾 ᳨ ฟ ⋡ 䛿 ᇵ 㣴 ἲ 䜘 䜚 㧗 䛟 䠈 㻼㻼㼂 䛿 㻠㻡㻚㻡 䡚 㻤㻣㻚㻜㻑䠈䜹䝑䝟ಀ ᩘ 䛿 㻜㻚㻢㻜䡚㻜㻚㻥㻞 䛷䛒䛳䛯䚹㻾㻲㻮㻿㻞㻠㼢㼑㼞㻚㻡 䜢㣗 ୰ ẘ 䛾ᚤ ⏕ ≀ ヨ 㦂 䛻
ᛂ ⏝ 䛩䜛䛯䜑䛻䛿䠈ᇵ 㣴 ᡂ ⦼ 䛸䛾㐪 䛔䜢ᨵ ၿ 䛩䜛ᚲ せ 䛜䛒䜛䛯䜑䠈㻾㻲㻮㻿㻞㻠㼢㼑㼞㻚㻡 䛾 㻯㼠 ್ 䛻ᇶ 䛵䛟⤖ ᯝ ุ ᐃ ἲ 䛾᭷ ⏝ ᛶ 䜢ホ ౯ 䛧䛯䚹 㻾㻲㻮㻿㻞㻠㻌 㼢㼑㼞㻚㻡 䛻䜘䛳䛶ୖ グ 㻟 㑇 ఏ Ꮚ 䛜᳨ ฟ 䛥䜜䛯㝿 䛾 㻯㼠 ್ 䛸ᇵ 㣴 ᡂ ⦼ 䛸䛾┦ 㛵 䜢᳨ ウ 䛧䛯䛸䛣䜝䠈㻾㻲㻮㻿㻞㻠㻌 㼢㼑㼞㻚㻡 㝧 ᛶ 䛷䠈䛛䛴ᇵ 㣴 㝧 ᛶ 䛾᳨ య ⩌ 䛿䠈㻾㻲㻮㻿㻞㻠㻌 㼢㼑㼞㻚㻡 㝧 ᛶ 䛷䠈䛛䛴ᇵ 㣴 㝜 ᛶ 䛾᳨ య
⩌ 䛸ẚ ㍑ 䛩䜛䛸䠈㻯㼠 ୰ ኸ ್ 䛜᭷ ព 䛻ప 䛛䛳䛯䠄㼜㻌㻨㻌 㻜㻚㻜㻡䠅䚹䛥䜙䛻䠈㻾㻲㻮㻿㻞㻠㻌 㼢㼑㼞㻚㻡 䛻 䜘䛳䛶᳨ ฟ 䛥䜜䛯 㻟 ᶆ ⓗ 㑇 ఏ Ꮚ 䛾 㻯㼠 ್ 䛜䠈㻾㻲㻮㻿㻞㻠㻌 㼢㼑㼞㻚㻡 㝧 ᛶ 䞉ᇵ 㣴 㝜 ᛶ ᳨ య ⩌ 䛾 㻯㼠 ್ 䛾 㻥㻡㻑ཪ 䛿 㻥㻥㻑ಙ 㢗 ༊ 㛫 ୗ 㝈 ್ 䜘䜚ప 䛔್ 䜢♧ 䛧䛯ሙ ྜ 䠈᭷ ព Ỉ ‽ 㻜㻚㻜㻞㻡 ཪ 䛿 㻜㻚㻜㻜㻡 䛾Ỉ ‽ 䛷ᇵ 㣴 ᡂ ⦼ 䛜㝜 ᛶ 䛷䛺䛔䛣䛸䜢♧ 䛧䛶䛔䜛䛣䛸䛛䜙䠈㻥㻡㻑ཪ 䛿 㻥㻥㻑 ಙ 㢗 ༊ 㛫 ୗ 㝈 ್ 䜢ᇵ 㣴 㝧 ᛶ ุ ู 㻯㼠 ್ 䛸䛧䛶௬ 㐺 ⏝ 䛧䠈㻾㻲㻮㻿㻞㻠㻌 㼢㼑㼞㻚㻡 䛾⤖ ᯝ ุ ᐃ 䜢⾜ 䛳䛯䚹䛭䛾⤖ ᯝ 䠈㻼㻼㼂 䛿 㻥㻠㻚㻣䡚㻝㻜㻜㻚㻜㻑䠈䜹䝑䝟ಀ ᩘ 䛿 㻜㻚㻤㻥䡚㻜㻚㻥㻡 䛸䛺䜚䠈᳨
ᰝ ⢭ ᗘ 䛜ᨵ ၿ 䛥䜜䛯䚹䛧䛯䛜䛳䛶䠈䛣䜜䜙 㻟 ᶆ ⓗ 㑇 ఏ Ꮚ 䛻≉ 䛧䛶䛔䜛䛜䠈㻾㻲㻮㻿㻞㻠㻌 㼢㼑㼞㻚㻡 䛷㝧 ᛶ 䠈䛛䛴ᇵ 㣴 ἲ 䛷㝜 ᛶ 䛸䛺䛳䛯᳨ య ⩌ 䛾 㻥㻡㻑ཪ 䛿 㻥㻥㻑ಙ 㢗 ༊ 㛫 ୗ 㝈 ್ 䛿䠈ᇵ 㣴 㝧 ᛶ ⤖ ᯝ 䜢 ண 䛧 䠈᳨ ᰝ ⢭ ᗘ 䜢 ᨵ ၿ 䛷䛝䜛ᣦ ᶆ 䛸䛧䛶᭷ ⏝ 䛷䛒䜛䛣䛸 䛜♧
䛥䜜䛯䚹䛣䜜䜙ୗ 㝈 ್ 䜢ᇵ 㣴 㝧 ᛶ ุ ู 㻯㼠 ್ 䛸䛧䛶㐺 ⏝ 䛩䜛䛣䛸䛷䠈ಙ 㢗 ᛶ 䛾㧗 䛔⤖
ᯝ 䜢ಖ ᡤ 䛻ᥦ ౪ 䛷䛝䜛䛣䛸䛜♧ ၀ 䛥䜜䛯䚹㻌
௨ ୖ 䠈≀ ⌮ ⓗ ◚ ○ 䜢䝧䞊䝇䛸䛧䛯᰾ 㓟 ᢳ ฟ ἲ 䛾 ే ⏝ 䛸㻾㻲㻮㻿㻞㻠㻌 㼢㼑㼞㻚㻡䛾㛤 Ⓨ 䛻䜘 䛳䛶䠈㻾㻲㻮㻿㻞㻠䜘䜚᳨ ฟ ឤ ᗘ 䛜ᨵ ၿ 䛧䠈㻱㻴㻱㻯䛸㻯㻚㼖㼑㼖㼡㼚㼕㻛㼏㼛㼘㼕䛻䛴䛔䛶䛿䠈ᇵ 㣴 ἲ 䛸ྠ
ឤ ᗘ 䠈ཪ 䛿䜘䜚㧗 䛔 ᳨ ฟ ឤ ᗘ 䜢ᚓ 䜛䜎䛷䛻ᨵ ၿ 䛥䜜䛯䚹 㻯㼠್ 䛻ᇶ 䛵䛟ุ ᐃ ἲ 䛿䠈㻯㻚㻌 㼖㼑㼖㼡㼚㼕䠈㼑㼍㼑㝧 ᛶ ⭠ ⳦ 䠈㼍㼟㼠㻭㝧 ᛶ ⭠ ⳦ 䛻≉ 䛧䛶䛔䜛䛜䠈ಙ 㢗 ᛶ 䛾㧗 䛔⤖ ᯝ 䜢
㎿ ㏿ 䛻ಖ ᡤ 䜈ᥦ ౪ 䛷䛝䜛ྍ ⬟ ᛶ 䛜♧ 䛥䜜䛯䚹㻾㻲㻮㻿㻞㻠㻌 㼢㼑㼞㻚㻡䛿䠈ᅜ ෆ 䛷Ⓨ ⏕ 䛩䜛
せ 䛺㣗 ୰ ẘ ⳦ 䛛䜙㞟 ᅋ 㣗 ୰ ẘ 䛾 Ⓨ ⏕ 㢖 ᗘ 䛜ప 䛔㣗 ୰ ẘ ⳦ ィ 㻝㻢⳦ ✀ 䜢 ⥙ ⨶ ⓗ 䛛䛴㎿ ㏿ 䛻≉ ᐃ 䛩䜛䛣䛸䛜ྍ ⬟ 䛷䛒䜚䠈㣗 ୰ ẘ ึ ື ㄪ ᰝ 䛾᪉ ྥ ᛶ 䛾Ỵ ᐃ 䜔ཎ ᅉ ✲
᫂ 䛾㎿ ㏿ 䠈䛥䜙䛻䛿⿕ ᐖ 䛾ᣑ 㜵 Ṇ 䜢ᐇ 䛩䜛ୖ 䛷᭷ ⏝ 䛺䝒䞊䝹䛸䛺䜛䚹 㻌 㻌
82 ㅰ ㎡ 㻌 㻌
㻌 ᮏ ◊ ✲ 䛻䛚䛔䛶䠈ᚚ ᣦ ᑟ 䛸ᚚ 㧗 㜀 䜢㈷ 䜚䜎䛧䛯ᅜ ❧ ་ ⸆ ရ 㣗 ရ ⾨ ⏕ ◊ ✲ ᡤ 㣗 ရ
⾨ ⏕ ⟶ ⌮ 㒊 㻌 ᮅ ᏹ 㻌 㒊 㛗 䛻ㅽ 䜣䛷ㅰ ព 䜢⏦ 䛧ୖ 䛢䜎䛩䚹䜎䛯䠈ᮏ ㄽ ᩥ స ᡂ 䛻䛒䛯 䜚䠈㈗ 㔜 䛺ຓ ゝ 䜢㈷ 䜚䜎䛧䛯ᖏ ᗈ ␆ ⏘ Ꮫ 䜾䝻䞊䝞䝹䜰䜾䝻䝯䝕䜱䝅䞁◊ ✲ 䝉䞁䝍䞊 㻌
ᅬ ஂ ⏕ ᩍ ᤵ 䠈ᒾ ᡭ Ꮫ ㎰ Ꮫ 㒊 ඹ ྠ ⋇ ་ Ꮫ ⛉ 㻌 ᑎ ᔱ ῟ 㻌 ᩍ ᤵ 䠈ᮾ ி ㎰ ᕤ Ꮫ ㎰ Ꮫ 㒊 ඹ ྠ ⋇ ་ Ꮫ ⛉ 㻌 ᯘ ㇂ ⚽ ᶞ 㻌 ᩍ ᤵ 䠈ᒱ 㜧 Ꮫ Ꮫ 㝔 㐃 ྜ ⋇ ་ Ꮫ ◊ ✲ ⛉ 㻌 ὸ
㕲 ኵ 㻌 ᩍ ᤵ 䛻῝ ㅰ 䛔䛯䛧䜎䛩䚹㻌
㻌 ◊ ✲ ⯡ 䛻㛵 䛧䛶ᚚ ᣦ ᑟ 䜢㈷ 䜚䜎䛧䛯ඖ ᓥ ᰿ ┴ ಖ ⎔ ቃ ⛉ Ꮫ ◊ ✲ ᡤ 㻌 ⚟ ᓥ ༤ 㻌 ༤ ኈ 䠈Ꮫ ⏦ ㄳ 䛾ᶵ 䜢 䛘䛶䛔䛯䛰䛝䜎䛧䛯ᓥ ᰿ ┴ ಖ ⎔ ቃ ⛉ Ꮫ ◊ ✲ ᡤ 㻌 ⏣ ཎ
◊ ྖ 㻌 㒊 㛗 䠈◊ ✲ ෆ ᐜ 䜔⤫ ィ ゎ ᯒ 䛻㛵 䛧䛶ᚚ ᣦ ᑟ 䜢㈷ 䜚䜎䛧䛯ᓥ ᰿ ┴ ฟ 㞼 ಖ ᡤ 㻌 㯮 ᓮ Ᏺ ே 㻌 㒊 㛗 䠈ᓥ ᰿ ┴ ಖ ⎔ ቃ ⛉ Ꮫ ◊ ✲ ᡤ 㻌 ᇛ ➼ 㻌 ᡤ 㛗 䛻ཌ 䛟䛚♩ ⏦ 䛧ୖ 䛢 䜎䛩䚹䛥䜙䛻䠈ᮏ ◊ ✲ 䛾ඹ ྠ ◊ ✲ ⪅ 䛷䛒䜛⚟ ᒸ ┴ ಖ ⎔ ቃ ◊ ✲ ᡤ 㻌 Ụ ⸨ Ⰻ ᶞ 㻌 ඛ
⏕ 䠈 ᾏ 㐨 ❧ ⾨ ⏕ ◊ ✲ ᡤ 㻌 ụ ⏣ ᚭ ஓ 㻌 ඛ ⏕ 䠈ᐩ ᒣ ┴ ⾨ ⏕ ◊ ✲ ᡤ 㻌 ⥥ ᘬ ṇ ๎ 㻌 ඛ
⏕ 䠈ຍ ⸨ ᬛ Ꮚ 㻌 ඛ ⏕ 䠈ᒣ ཱྀ ┴ ⎔ ቃ ಖ 䝉䞁䝍䞊㻌 ㄪ ᜏ ᫂ 㻌 ඛ ⏕ 䠈ட ᒣ ග ༤ 㻌 ඛ ⏕ 䠈 බ ┈ ㈈ ᅋ ἲ ே ᕞ ⏕ ά ⛉ Ꮫ 䝉䞁䝍䞊㻌 ᇼ ᕝ ⨾ 㻌 ඛ ⏕ 䠈ඖ ᾏ 㐨 ❧ ⾨ ⏕ ◊ ✲ ᡤ 㻌 ᒣ ཱྀ ᩗ 㻌 ඛ ⏕ 䠈ᚋ ⸨ Ⰻ ୍ 㻌 ඛ ⏕ 䛻῝ ㅰ 䛔䛯䛧䜎䛩䚹㻌
㻌 ᖖ 䛻䠈ᮏ ◊ ✲ 䜢ᚚ ᨭ 䛟䛰䛥䛳䛯ᓥ ᰿ ┴ 㣗 ⫗ ⾨ ⏕ ᳨ ᰝ ᡤ 㻌 ᮡ ᶫ ❶ ⩏ 㻌 ᡤ 㛗 䜢䛿䛨 䜑䛸䛧䛯㣗 ⫗ ⾨ ⏕ ᳨ ᰝ ᡤ 䛾ⓙ ᵝ 䠈ᓥ ᰿ ┴ ಖ ⎔ ቃ ⛉ Ꮫ ◊ ✲ ᡤ 䛾ⓙ ᵝ 䛻ឤ ㅰ 䛾ព 䜢⾲ 䛧䜎䛩䚹㻌
㻌 ᭱ ᚋ 䛻䠈䛔䛴䜒⚾ 䛾䛣䛸䜢⌮ ゎ 䛧䠈ບ 䜎䛧䛶䛟䜜䛯⚾ 䛾ᐙ ᪘ 䠈䛭䛧䛶䠈ஸ 䛝∗ 䛻ᚰ 䛛 䜙䛾ㅰ ព 䜢グ 䛧䜎䛩䚹
ᘬ ⏝ ᩥ ⊩
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