Abs Absorbance
APS Ammonium Peroxodisulfate
BPB Bromophenol Blue
CE Cyanoethyl
CPG Controlled Pore Glass
DMF N,N-dimethylformamide
DMSO Dimethyl Sulfoxide
DMTrCl 4,4'-Dimethoxytrityl Chloride
DNA Deoxyribonucleic Acid
DNase Deoxyribonuclease
dsRNA double-strand RNA
EDTA Ethylenediamine-N,N,N',N'-tetraacetic Acid
GNA Glycol Nucleic Acid
HIV Human Immunodeficiency Virus
3-HPA 3-Hydroxypicolinic Acid
HPLC High Performance Liquid Chromatography
MALDI-TOF Matrix Assisted Laser Desorption Ionization-Time of Flight
mRNA messenger RNA
miRNA micro RNA
MS Mass Spectrometry
NMR Nuclear Magnetic Resonance
66
ON Oligonucleotide
OD Optical Density
PAGE Polyacrylamide Gel Electrophoresis
PBS Phosphate Buffered Saline
pH potential Hydrogen, power of Hydrogen
RCY Radiochemical Yield
RISC RNA-induced silencing complex
RNA Ribonucleic Acid
RNase Ribonuclease
rRNA ribosomal RNA
rt room temperature
shRNA small hairpin RNA, short hairpin RNA
siRNA small interfering RNA
snRNA small nuclear RNA
TBAF Tetrabutylammonium Fluoride TBDMS tert-Butyldimethylsilyl
TBE Tris-Borate-EDTA
TBTA Tris[(1-Benzyl-1H-1,2,3-triazol-4-yl)methyl]amine
TEAA Triethylammonium Acetate
TEMED N,N,N',N'-Tetramethylenediamine
THF Tetrahydrofuran
TLC Thin Layer Chromatography
Tm Melting Temperature
67
TMS Trimethylsilyl
tRNA transfer RNA
UV Ultraviolet
VIS Visible
XC Xylene Cyanol FF
68
ᘬᘬ⏝ᩥ⊩
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ㅰ ㅰ㎡
ⴭ⪅䜢ᮏ◊✲䜈䛸ᑟ䛝䚸㐙⾜䛩䜛䛻䛒䛯䜚⤊ጞᚚ᠓⠜䛺䜛ᚚᣦᑟ䠈ᚚ㠴᧡䜢㈷䜚䜎 䛧䛯ᒱ㜧ᏛᏛ㝔㐃ྜ⸆་⒪ሗ◊✲⛉ᩍᤵ㻌 ฟᖾኵඛ⏕䛻⾺ᚰ䜘䜚ឤㅰ 䛔䛯䛧䜎䛩䚹
ㄽᩥ䜢ᇳ➹䛩䜛䛻䛒䛯䜚䚸ከ䛺䜛ᚚຓゝ䛺䜙䜃䛻ᚚᨭ䜢㈷䜚䜎䛧䛯ᒱ㜧Ꮫ
Ꮫ㝔㐃ྜ⸆་⒪ሗ◊✲⛉ᩍᤵ ụ⏣ᑗඛ⏕䛻῝䛟ឤㅰ⮴䛧䜎䛩䚹
ᮏ◊✲䛾ィ⏬䚸ᐇ㦂䚸⪃ᐹ䛾⣽㒊䚸ཎⴭㄽᩥ䛾సᡂ䛻⮳䜚ከ䛺䜛䛤ᣦᑟ䜢㈷䜚䜎 䛧䛯ᒱ㜧ᏛᕤᏛ㒊⏕ᕤᏛ⛉ຓᩍ㻌 ႐ከᮧᚨඛ⏕䛻῝䛟ឤㅰ⮴䛧䜎䛩䚹
䛥䜙䛻䚸ᮏ◊✲䜢⾜䛖㝿䛻ᚚຓゝ䚸ᚚ༠ຊῧ䛘䜢㈷䜚䜎䛧䛯ᒱ㜧⸆⛉Ꮫྡᩍᤵ
䠄ඖᒱ㜧ᏛᕤᏛ㒊⏕ᕤᏛ⛉≉ู༠ຊ◊✲ဨ䠅 ∦ᒸ㈆ඛ⏕䛻῝䛟ឤㅰ⮴䛧䜎䛩䚹
䜎䛯䚸ከ䛺ᚚ༠ຊ䛸ᚚຓゝ䜢㡬䛝䜎䛧䛯ᒱ㜧ᏛᕤᏛ㒊≉௵ຓᩍ㻌 ᰘ⏣⥤ඛ⏕䚸
ⓑ▼Ꮥ㞝༤ኈ䚸୰ᓥ♩⨾ಟኈ䚸⥘ሙెዉಟኈ䛻῝䛟ឤㅰ⮴䛧䜎䛩䚹
䛭䛧䛶䚸ᮏ◊✲䜢㐙⾜䛩䜛䛻䛒䛯䜚䚸◊✲άື䜢ඹ䛻䛧䛶䛝䛯䚸ᒱ㜧ᏛᕤᏛ㒊⏕
ᕤᏛ⛉⏕యᛂᕤᏛㅮᗙฟ◊✲ᐊ䛾Ꮫ⏕ⓙ䛥䜣䛻῝䛟ឤㅰ⮴䛧䜎䛩䚹
㻌 ᮏㄽᩥ䛾ᑂᰝ䛻䛒䛯䜚᭷┈䛺ᚚຓゝ䜢㈷䜚䜎䛧䛯ᒱ㜧ᏛᏛ㝔㐃ྜ⸆་⒪
ሗ◊✲⛉ᩍᤵ㻌 ㉥ᑿᖾ༤༤ኈ䚸ᒱ㜧ᏛᏛ㝔㐃ྜ⸆་⒪ሗ◊✲⛉ᩍᤵ
⩚㞞அ༤ኈ䚸ᒱ㜧ᏛᏛ㝔㐃ྜ⸆་⒪ሗ◊✲⛉‽ᩍᤵ ụ⏣ᑗ༤ኈ䛻ཌ䛟