《農芸化学奨励賞》 受賞者講演要旨 - 日本農芸化学会

DNA

1.

DNADNApolῌ

polas¹³ !"#$%&$'()*+ ,-^{(in vitro)} .!$%/^pol DNA ! 012"

#2$34567()*89&:()* %^{(in vivo)}

;<=*&'()> /?*;@) (&$'())+ AB/;+C*,-.D/5 E)F7( GHIJK 87(0)*B85LM+ B 12 NO3PQRST(Gene Knockout)U;4&*N. PQRST(Chemical Knockout)UVW56XWY 78;Z9$@:[2\[2;<=]*B8$

% pol^_>7)?E5L@C8`A]*+ a%;b 0^_87( pol,-.DcB$%>7) CcB5E)FCd*BV*+

ῌ^polD8%E;7$ef $@F gGH!;IJ5KC*MhLMa:())+ ABNi O^pol^_6$%H!a:McBV*^pol5P7 (Y%'M+ BXj^polklCm(5An4()

*NiOk$;> pol.D/^_Ni5o 7()*+ Q^_¹⁴R[;&M'(pS7(<W TUV W$%/,-.D/5XY7( qr;Z[

.D\5E)F7()*+ ]^_#5s;^m*+ 2. pola Dehydroaltenusin

2.1 Dehydroaltenusin !"#$

tut(Taxus cuspidata)_`;v ab 7()*

w2cw5d2eG7( Afx5yQz{|

7M8Bn Acremoniumg}~;^pola.DcBV' M+ AB Afx.u€{h@$%cB5 id2H!7( .j5!7M+ A"# Q\‚

ƒ„{^Lk…†.D/87(lma:()*‡?\

DehydroaltenusinV'M n¹+ 2.2 Dehydroaltenusin^pol%&

id2H!a:MDehydroaltenusin ῌop^pol jˆ^DNA !‰^polV*^polaF=5qŠ;

.D7Mn¹+‡?^pola.D/Aphidicolinrs V* polaF=>^pold8^poleYe:;.D7(7 rj+ Dehydroaltenusin^IC50t^0.68mBVW ‡?

pola.D/XWY²⁰uY‹>.D7M+ pola;

Š*v@ŒJzPT$%a:* wxHŽ

{y‘{Œ^(Biacore);X*!$% Q\^polcB 5rC*^p180ŒJzPT;"C*B85E)F7M+ B :%B8$%Dehydroaltenusin pol a^_H IJ;*8’4%:*+

2.3 Dehydroaltenusin'()*+,-./%&

Dehydroaltenusin“T”•cz(HeLa cells){|5 }~–;€7Mn¹+A^LD50tAphidicolinX WY>^{(38mB), pol a}.DcB‹a5‚—7()*B8

ƒ„a:M+rMQ\…˜™²⁴:[;<)(cz†`

5^S`DNA`‡ˆaecz^DNA‰Š.

š‹a:M+B:XWpola^DNA !›&:*^S`Œ

)(<WAcB.Da::œRT„yczŽ

5C*8’4%:*+

2.4 Dehydroaltenusin012%&

Dehydroaltenusin‘’,5’“C*;VMW r?.

125ž”7(;;Ÿ7M+ QT U8e}~^{pol a}cB5.D7 HeLacz{|5€

C*B85ž•7M+

–; o¡Sy¢^HeLacz5£—C*B8;XW¤¥

˜῍5¥aeM™ Dehydroaltenusin5™P Cš

;›$7($%^{20 mg/kg}5œsž 7(˜῍%Ÿ

 .5¡G7M+ Q.™P¦Cš5™P7M0{

TI§¦8¢m( £¨;˜῍{.5€7MB8$

% “T”•op¤¥˜῍;+C*©˜῍cB5E)F7 Mn²+0{TI§¦¡SyŽ¤™/¥ª5š‹7M

8BnDehydroaltenusin™P¦¥ª;Š8E%:

$'MB8 ¦¦¡Sy%§«: .¬¨V'MB8

$% Q.™P;X*©ª0)B8ƒ„a:M+ a

%; ¦¦˜῍$«¬Š5ª!7(¥­š‹7M8Bn Dehydroaltenusin™P¦˜῍cz­QI„y ®Ž

5¯B7()M+

"#$% polaqŠ.D/©ª0)©•/

;*B8`Aa:*+

3. poll Petasiphenol 3.1 Petasiphenol !"#$

š VV*°]8j(Petasites japonicus)±

§h@x;^poll,-.DcBE%:M+ QcB5®¯;

7(.D\5id2H! .j5!7M"# ²³

§´.PetasiphenolV'M n³+ Q\

1994R; µW°]8j$%ida:M‡?\V' M+

3.2 Petasiphenol^pol%&

id2H!a:MPetasiphenol ῌop^pol jˆ^DNA"#2$34;¶PC*^poll5qŠ;.D 7 A^IC50t^7.8mBV'M n³+ polbῌlῌm Npol X²·‚U 8¸œ: R‚°±²µ.?*³ 7()* Q\^polb5.D7)B8¹h(º´µ )+ Biacore!$%Petasiphenol^C-¶·^polcBo t{;"ef N-¶·^BRCTot{;"C*B8

¸».¹º» ¼»6¼½K½

3 ³⁶

῍1 pola Dehydroaltenusin

῍2 Dehydroaltenusin

!" # $%&'() * ῌ ³⁷

poll-BRCT Petasiphenol

!^poll"#$ %&'()*+,-.

3.3 Petasiphenol

/012%34"# 536!TPA (12 -O-tetra- decanoylphorbol-13-acetate)7#34()8$

9! :; Petasiphenol^250mg<=>

%34"#$+!

4. Curcumin poll

4.1 Curcumin

Petasiphenol?@ABCD<EF GH

I()^Curcumin JKKL ! M!N

-.)^CurcuminOPQRSDTU

$MV13JCompound3W15LUUX!JY 4L

4.2 ^poll

Z[\O]^_!Petasiphenol (Compound 1), NCurcumin (Compound 2)^Curcumin (Compound3W15)^poll&'"#%34"#$`!

:;*.O@ABCD^15poll&'

"#%34"#abcdO. JY⁴L ^Cur- cuminPetasiphenolFef"#$+! -O^Cur-

cuminFe"#f$ghdi!:<

Compound 13 (Monoacetyl curcumin)jf<E kl;O pol l&'6%346>F\)m # +,-.) n Monoacetyl curcumin%34o

!p!q"<[)

5.

#$%"#E)P^pol&rQ$s'()*

F pol&rQt(>&'$du(*<[ v we*\>)&rxD (>y z&r {|

JDT}*L~ $)*F P^pol&rQ+

€)x ],‚$ƒ-![

*.n<^pol&'6 %„6…%016!p!

-.) (†^pol&rQ$^nM J‡B]ˆL ‰

Š<&'()‹.# J/ŒL fg ) z&'6

ŽInhibitor~<E0pol&rQt(&'6/

Œ>‘’&'6!……1g z“

ŽSuppressor~ }”) vw pol&rQt(&'6

•%^pol&'6–2$3! —˜<h>o] D™p!h>)*q"-.)

š› T4]5Tœ]ž!Ÿ !6¡

7¢5T7£T4¤¢T¢€p¥! ¦§Ÿ 

<E)¨©Tœ5Tª8T4ª8T¢€!9«¬!

n( š›­g0®¯°$±*²g 3 pollt(&'"#$()Petasiphenolx ƒ³

´ :µDT¶;· ¸ ¹·º»¼<½

³⁸

!"#

$%!&'() *$+%,-./0123456 78 97(:;7%! &'(<=$

%,>?/@/A/BA/C(DE567 F!GH(IJKL( ! MF!NOPQ (RST*$%!&

'() *$+%,-./(UVW56 X Y56 Z[\]^5(_`7 a/bc(dJe f$ghij7T! kT,l(

m-./0UVC(/@`$%no pq rs& -!/a/<0 rtu"#$%

rs& &va/wxsyuu7T,z^ !{

8 >?/@/A/BA/C$

R|^}'~sPu(€ r s& ‚)‚ƒ„*

…†‡ˆ<0 rtu T‰Š7&'(R|^}$%,

‹s+,u-Œ C(x`0'Ž`7 ./L

Y‘’0(l >?/

@/(“1/2T‰Š7”2•3–27 457

Y(—(6˜P ™7š›

,{TLœ879:,{;< +žŸ

7 z0¡=L¢£0¤›7M¥‰l ¦

§7T!¨ ©ª7«L>?¬

a/­® 20v¯_`T‰Š7°_`7±LN OPQ

῍4 Curcumin@A(poll²³œ´µ¶·œ´

¸ª*¹ºB» ¼ ?¬a/©ª ½ ῌ ³⁹