2012 6
2
東京大学大学院理学研究科
小澤 岳昌
4
4
細胞構造生物学
in-cell NMRを用いたアプローチ首都大学東京大学院理工学研究科
伊藤 隆
10 世界最大規模の食中毒シガテラを予防する
抗シガトキシン抗体の作製とシガトキシンの微量検出法の開発
大阪府立大学大学院理学研究科
円谷 健
18
細胞外マトリックスのペプチド科学
メラニンの分子解剖と医薬分野への応用
東京薬科大学薬学部
野水 基義
23
東京大学大学院理学研究科化学専攻 博士課程１年
桂 嘉宏
名古屋大学大学院工学研究科化学・生物工学専攻 博士後期課程３年
内藤 豊裕
30
ドイツ・マックスプランク分子生理学研究所留学体験記
Max Planck Institute of Molecular Physiology, Department of Chemical Biology
高山 浩
34
第６回 バイオ関連化学シンポジウム（第 27 回 生体機能関連化学シンポジウム、第 15 回 バイオテクノロジー部会シンポジウム、第 15 回 生命化学研究会シンポジウム）
第 39 回 国際核酸化学シンポジウム (ISNAC2012)
アンチセンス・遺伝子・デリバリーシステムシンポジウム 2012
第２回 アジアケミカルバイオロジー会議（ACBC2）（第 15 回生命化学研究会）
編集後記
4 3
10
IF
20
2
in-cell NMR
300 400 mg/mL
macromolecular crowding [1]
in vitro
NMR
in-cell NMR
[2] in-cell
NMR
[3,4] in-cell NMR
in-cell NMR In-cell NMR
NMR
in vivo NMR
1990
NMR
13C 15N
13C/15N
NMR
NMR
2001 UCSF Dötsch
in-cell 2D 1H-15N HSQC
in-cell NMR [5]
in-cell NMR
in-cell NMR [6,7] 2009
in-cell NMR [8]
[9] 1 in-cell NMR
In-cell NMR
[10]
In-cell NMR
2 1H-15N NMR
NMR NOE
in vitro
in-cell NMR
NMR 3D/4D NMR 1 In-cell NMR
2 [17]
3D/4D NMR in vitro
In-cell NMR
http://www.thermus.org/j_index.htm 100
20 in-cell NMR
NMR TTHA1718
66
6 3 3
NMR
NMR NMR NMR
6 85%
6
3 NMR
in vitro
nonlinear sampling
maximum entropy
[4,11,12]
1/8
3 NMR
NMR 2
NMR 2
2 TTHA1718 NMR
1H-15N HSQC
NMR NMR
Leu41 A46
3 TTHA1718 1H
1H-13C HMQC
NOE
1H/13C 2H
NMR [13]
Stokes-Einstein-Debye in vitro
NMR
1H 3D 13C/13C-separated NOESY
NOE
13C/15N 3D 15N-separated NOESY 3D 13C-separated NOESY
NOE
13C
2 2
Peter Güntert
CYANA v3.0[14]
RMSD 0.96 Å 4
NOE NOE
4
TTHA1718 C&EN Chemical Year in
Review 2009 9 1
in-cell NMR
TTHA1718 in-cell NMR
TTHA1718 3 4 mM
in-cell NMR 4
TTHA1718 NOE
protein G B1 GB1 CYANA
100 20
20
Tau 5 µM [15]
in-cell NMR macromolecular crowding 2
NMR
NMR
TTHA1718 in-cell NMR
1/10 200 500 µM protein G B1 GB1 66
1H
maximum entropy NOESY
NMR [16] TTHA1718
4 TTHA1718
GB1 2
In-cell NMR 2
in-cell NMR
intrinsically disordered proteins (IDPs)
macromolecular crowding [18]
[15]
in-cell NMR
in-cell NMR
[8]
15N
In-cell NMR
NOESY
NMR
1. Ellis, R.J., Trends. Biochem. Sci. 26, 597-604 (2001).
2. Ito, Y. & Selenko, P., Curr. Opin. Struct. Biol. 20, 640-648 (2010).
3. Sakakibara, D. et al., Nature 458, 102-105 (2009).
4. Ikeya, T. et al., Nat. Protoc. 5, 1051-1060 (2010).
5. Serber, Z. et al., J. Am. Chem. Soc. 123, 2446-2447 (2001).
6. Selenko, P. et al., Proc. Natl. Acad. Sci. USA 103, 11904-11909 (2006).
7. Sakai, T. et al., J. Biomol. NMR 36, 179-188 (2006).
8. Inomata, K. et al., Nature 458, 106-109 (2009).
9. Ogino, S. et al., J. Am. Chem. Soc. 131, 10834-10835 (2009).
10. Serber, Z. & Dotsch, V., Biochemistry 40, 14317-14323 (2001).
11. Barna, J. C. J. et al., J. Magn. Reson. 73, 69-77 (1987).
12. Rovnyak, D. et al., J. Magn. Reson. 170, 15-21 (2004).
13. Rosen, M.K. et al., J. Mol. Biol. 263, 627-636 (1996).
14. Güntert, P., Prog. Nucl. Mag. Res. Sp. 43, 105–125 (2003).
15. Bodart, J.F. et al., J. Magn. Reson. 192, 252-257 (2008).
16. Ikeya, T. et al., J. Biomol. NMR 50, 137-146 (2011).
17. Burz, D. S. et al., Nat. Methods. 3, 91–93 (2006).
18. Dedmon, M. M. et al., Proc. Natl. Acad. Sci. USA 99, 12681-12684 (2002).
5
1), 2)
5 6 7 8 9 13
30 3 nm 1 .
1989 3)
2001 CTX3C
4), 5), 6)
O O O O
O
O O
O OH OH
H
H H H H H
H H
H H O H
O
O O
O
H H H H H
H H H H H
OH B C D
E F
H I
K
M A
R1
R2 H
HO OH CTX1B(1) : R1 = CTX3C (2) : R1, R2 = H, n = 1
, R2 = OH, n = 0
G J
L n
3 nm
51-hydroxyCTX3C (3) : R1 = H, R2 = OH, n = 1
HO OH
54-deoxyCTX1B (4) : R1 = , R2 = H, n = 0
477 Å2 (A) HRP
(B)
7), 8), 9), 10)
CTX1B (1 µg)
11)
CTX1B CTX1B
CTX1B
12), 13)
1 (A) E 4
CTX3C (2) CTX3C (2)
200 400 Å2
CTX3C (2) 3 4 5
(ABC ABCD ABCDE )
253 318 398 Å2 2
ABC: 253 Å2 ABCD: 318 Å2
ABCDE: 398 Å2 IJKLM: 477 Å2
CTX3C(2)
5 ABCDE
5 5 5 ABCDE
398 Å2
400 Å2
5 A E
5 CTX3C
CTX3C 5 6 IJKLM
:477 Å2 5 6 CTX3C(2)
5 6 BSA
KLH
7), 9)
5 5 KLH 7 5 3
7 BSA
8 6
IgG IgG+M
6
(Kd) 6 5
11 Kd 0.8 nM 10.8 nM
10C9 10C9 4 12 Kd 1.9 µM 11
2000 3 13 73.6 µM 4 12
30 1
CTX3C 14
O OH HO
H H
OH H HOH
H D C B
A O O
O H
H E
R O
O O
O O O
O H
H H H
O OH
H H H H
I K
M HO O
7: R = KLH
8: R = BSA 9: R = KLH
10: R = BSA J
L
5 6
O OH HO
H H
OH H HOH
H D B C
A O O
O H
H E
OH O
n
O O
O O O
O H
H H H
O OH
H H H H
I K
M R O
J L
n
CTX3C IJKLM 6 KLH 9
IgG 3D11 3D11 5 14
ELISA Kd 5
14 Kd 4 nM 3D11 CTX3C
Kd 122 nM
A (Kd = 43 µM) B
8), 9)
E 4 1
4
M OH
(CTX1B 51-hydroxyCTX3C )
5
> 400~500 Å2 M OH
6 15 5 15 KLH BSA
16, 17 KLH 16 4
3
7C9, 8B9, 8H4 HIJKLM 18 (Kd) ELISA
407, 108, 48 nM 8H4
8H4 CTX1B Kd =
20.4 nM 51-hydroxy- CTX3C Kd = 13.6 nM
M OH
O O
O O
O O O
O O O O OHC
H H H H H H H H H
H H H H H H
H OH
F D
I H A
C B O
O O
O O
O O
O O
O O O H H H H H H H H H
H H H H
H H H OH
O
B A D C F H I
K E
G J
O OH HO
H H
OH H HOH
H D C B
A O OH
OH H
H
E O
O H HO O
OH H H
OH H HO
OMe
A B C
O OH HO
H H
OH H HO
OH H OH
H D B C A
11 12 13
HO O
O O
O O OH
H H H H H
H H H
HO
I K
M O
O HO2C
O O
O O
OH O H
H H
HOH
HOH
H H
OH B
A C D F
O
O O
O
O O
O OHH
OH
H H H HH
H H
H O
O O
H OH
O H
H
O
O H
H
O
O
OH OH
O HOH O O
H HO
H
OHH
OH H
H OH
H OH O
O O
O OH
OH
H
HOH H H H
H H OHH
H H NaO3SO
OH
O O
O O
O HO
HO OH
OH HO
H H H
OHH H H H H OSO3Na OH
HO OH
O
O H
H OH
O
O H
H
H H
H H HO
OH H
HO O P
K F
D C
B H
T
S R
Q
N M I
A
Y X W
V U
14 J
L
Brevetoxin A (BTX A) Brevetoxin B (BTX B)
J
G E
Okadaic acid (OA)
L G J
E F' E' D' C' B'
A' Z
Maitotoxin (MTX) G
E
O O Me Me OH O OH O O
Me
Me H H H
H H H H HO J
L Me
H
I K
M O O H
R O
16: R = KLH 17: R = BSA
O O OH Me Me O OH O O
Me
Me H H H
H H H HOH J
L Me
H
I K
M HOHO H
18 O O
OH Me Me O OH O O
Me
Me H H H
H H H
HOH J
L Me
H
I K
M O O H
R O
n 15: R = OH
CTX3C Kd = 3.2 µM 8H4 M OH
8H4 (BTX-A, BTX-B, OA, MTX)
CTX1B
51-hydorxyCTX3C .
10)
A
CTX1B CTX1B
CTX1B 12)
CTX1B A
CTX1B CTX1B
5 19
19
DTSSP BSA KLH
TCEP
BSA KLH
22 (BSA) 23 (KLH) 22
23 19
BSA KLH 24
25 KLH
25 4
ELISA BSA 24 IgG
3G8
3G8 ABCDE 26 (Kd) ELISA 1.5 nM
3G8 CTX1B Kd = 14.9
nM A CTX3C
3G8 A
3G8 ABC 27 Kd 410 nM ABCDE 26
270 3G8 CTX1B (1)
ABCDE 3G8
(BTX-A, BTX-B, OA)
O
O O
H H
H H OH
O O
HO
H H H
H H
HO H OH
O NH
O N O
O
19
S O
O N
O
O
NaO3S S R
O
O
O O
H H
H H OH
O O
HO
H H H
H H
HO H OH
O NH
O N O
O S
O R S
O O N
O
O
NaO3S S O
O N O
O SO3Na DTSSP
20: R = BSA (n = approx.13) 21: R = KLH
HS R
O
22: R = BSA 23: R = KLH 24: R = BSA (n = approx.10)
25: R = KLH
BSA, PBS buffer or KLH, PBS buffer
TCEP PBS buffer 14, DMF
PBS buffer
A B C D
E
A B C D
E
n
n n
O
O O
H H
H H OH
O O
HOH
H H H
H H
HO H
OH 26
A B C D
E O
O O
H H
H H H OH HO OH
27
A B C
7), 8), 9), 10)
2
3 nm
2 2
4 7
CTX1B
3G8
8H4 CTX1B
ELISA 3G8 CTX1B
8H4
0.28 ng/mL CTX1B 8
CTX3C
CTX1B
CTX3C
10C9 3D11
51-hydroxyCTX3C 10C9
8H4 CTX3C 51-hydroxyCTX3C
54-deoxyCTX1B 3G8 3D11
[CTX1B] (µM)
Absorbance (490 nm)
14)
1994
17~8
1) Scheuer, P. J., Ciguatera and its off-shoots chance encounters en route to a molecular structure, Tetratedron 1994, 50, 3.
2) Lewis, R. J., The changing face of ciguatera, Toxicon 2001, 39, 97.
3) Murata, M., Legrand, A. M., Ishibashi, Y., Yasumoto, T., Structures of Cigutoxin and Its Congener, J. Am.
Chem. Soc. 1989, 111, 8929.
4) Hirama, M., Oishi, T., Uehara, H., Inoue, M., Maruyama, M., Oguri, H., Satake, M., Total Synthesis of Cigutaoxin CTX3C, Science 2001, 294, 1904.
5) Inoue, M., Miyazaki, K., Uehara, H., Maruyama, M., Hirama, M., Frist- and second-generation total synthesis of ciguatoxin CTX3C, Proc. Natl. Acad. Sci. USA 2004, 101, 12013.
6) Inoue, M., Miyazaki, K., Ishihara, Y., Tatami, A., Ohnuma, Y., Kawada, Y., Komano, K., Yamashita, S., Lee, N., Hirama, M., Total Synthesis of Cigatoxin and 51-HydroxyCTX3C, J. Am. Chem. Soc. 2006, 128, 9352.
7) Oguri, H., Hirama, M., Tsumuraya, T., Fujii, I., Maruyama, M., Uehara, H., Nagumo, Y. , Synthsis-Based Approach toward Direct Sandwich Immunoassay for Ciguatoxin CTX3C, J. Am. Chem. Soc. 2003, 125, 7608.
8) Tsumuraya, T., Fujii, I., Inoue, M., Tatami, A., Miyazaki, K., Hirama, M., Production of monoclonal antibodies for sandwich immunoassay detection of ciguatoxin 51-hydroxyCTX3C, Toxicon 2006, 48, 287.
9) Tsumuraya, T., Fujii, I., Hirama, M., Production of monoclonal antibodies for sandwich immuoassay detection of Pacific ciguatoxins, Toxicon 2010, 56, 797.
10) Tsumuraya, T., Takeuchi, K., Yamashita, S., Fujii, I., Hirama, M., Development of a monoclonal antibody against the left wing of ciguatoxin CTX1B: Thiol strategy and detection using a sandwich ELISA, Toxicon 2012, doi:10.1016/j.toxicon.2012.04.347.
11) Hokama, Y., Hong, T. W. P., Isobe, M., Ichikawa, Y., Yasumoto, T., Cross-Reactivity of Highly Purified
Okadaic Acid (OA), Synthetic, Spiroketal East Sphere of OA and Cigutoxin, J. Clinical Anal. 1992, 6, 54.
12) Oguri, H., Tanaka, S., Hishiyama, S., Oishi, T., Hirama, M., Tsumuraya, T., Tomioka, Y., Mizugaki, M., Designed Hapten Aimed at Anti-ciguatoxin Monoxlonal Antibody: Synthsis, Immunization and Discrimination of the C2 Configuration, Synthsis 1999, 1431.
13) Nagumo, Y., Oguri, H., Shinodo, Y., Sasaki, S., Oishi, T., Hirama, M., Tomioka, Y., Mizugaki, M., Tsumuraya, T., Concise Synthesis of Ciguatoxin ABC-Ring Fragments and Surface Plasmon Resonance Study of the Interaction of Their BSA Conjugates with Monoclonal Antibodies, Bioorg. Med. Chem. Lett.
2001, 11, 2037.
14) Inoue, M., Lee, N., Tsumuraya, T., Fujii, I., Hirama, M., Use of monoclonal antibodies as an effective strategy for treatment of ciguatera poisoning, Toxicon 2009, 53, 802.
Plate assay!
A10!
A13
!
A24!
A119!
A51!
A64!
B98!
B31!
AG10!
B54!
B133!
A167!
B160!
C28!
C64!
C68!
AG32!
A99!
C16!
AG73!
A208!
MRGSGTGAALLVLLASVLWVTVRSQQRGLFPAILNLATN!
AHISANATCGEKGPEMFCKLVEHVPGRPVRHAQCRVCDG!
NSTNPRERHPISHAIDGTNNWWQSPSIQNGRE!
ARKAKNSVSSLLSQLNNLLDQLGQLDTVDLN!
KLNEIEGSLNKAKDEMKASDLDRKVSDLESEARKQEAAI!
MDYNRDIAEIIKDIHNLEDIKKTLPTGCFNTPSIEKP!
A1! A2! A3!
A4!
A5! A6! A7!
A8! A9! A10!
C154! C155!
C156!
C157!
C158! C159!
C160! C161!
C162! C163!
C164! C165!
673 Synthetic peptides
!
Laminin-1! α1鎖!
β1鎖!
γ1鎖!
Screening of cell adhesive peptides !
細胞�
�
!
!
P D
P E T I E
P E P I T E D
-
E!
I!T!
D!
E!
P!
E! P!
I!T!
D!
E!
P! P!
E! T!
I!
D!
E!
P!
P!
E! T!I! D!
E!
P!
P!
1) Yamada, Y. & Kleinman, H. K. (1992) Current Opin. Cell Biol., 4, 819-823.
2) Nomizu, M., Kim, W. H., Yamamura, K., Utani, A., Song, S. -Y., Otaka, A., Roller, P. P., Kleinman, H.
K., & Yamada, Y. (1995) J. Biol. Chem., 270, 20583-20590.
3) Nomizu, M., Kuratomi, Y., Song, S.-Y., Ponce, L. M., Hoffman, M. P., Powell, S. K., Miyoshi, K., Otaka, A., Kleinman, H. K., & Yamada, Y. (1997) J. Biol. Chem., 272, 32198-32205.
4) Nomizu, M., Kuratomi, Y., Malinda, K. M., Song, S.-Y., Miyoshi, K., Otaka, A., Powell, S. K., Hoffman, M. P., Kleinman, H. K., & Yamada, Y. (1998) J. Biol. Chem., 273, 32491-32499.
5) Nomizu, M., Kuratomi, Y., Ponce, L. M., Song, S. -Y., Miyoshi, K., Otaka, A., Powell, S. K., Hoffman, M. P., Kleinman, H. K., & Yamada, Y. (2000) Arch. Biochem. Biophys., 378, 311-320.
6) Suzuki, N., Nakatsuka, H., Mochizuki, M., Nishi, N., Kadoya, Y., Utani, A., Oishi, S., Fujii, N., Kleinman, H. K., & Nomizu, M. (2003) J. Biol. Chem., 278, 45697-45705.
7) Hoffman, M. P., Nomizu, M., Roque, E., Lee, S., Jung, D. W., Yamada, Y., & Kleinman, H. K. (1998) J.
Biol. Chem., 273, 28633-28641.
8) Kadoya, Y., Nomizu, M., Sorokin, L. M., Yamashina, S., & Yamada, Y. (1998) Dev. Dyn., 212, 394-402.
9) Hoffman, M. P., Engbring, J. A., Nielsen, P. K., Vargas, J., Steinberg, Z., Karmand, A. J., Nomizu, M., Yamada, Y., & Kleinman, H. K. (2001) J. Biol. Chem., 276, 22077-22085.
10) Hozumi, K., Suzuki, N., Nielsen, P. K., Nomizu, M., & Yamada, Y. (2007) J. Biol. Chem., 281, 32929-32940.
11) Mochizuki, M., Kadoya, Y., Wakabayashi, Y., Kato, K., Okazaki, I., Yamada, M., Sato, T., Sakairi, N., Nishi, N., & Nomizu, M. (2003) FASEB J., 17, 875-877.
12) Hozumi, K., Yamagata, N., Otagiri, D., Fujimori, C., Kikkawa, Y., Kadoya, Y., & Nomizu, M.(2009) Biomaterials, 30, 1596-1603.
13) Hozumi, K., Sasaki, A., Yamada, Y., Otagiri, D., Kobayashi, K., Fujimori, C., Katagiri, F., Kikkawa, Y., Nomizu, M. (2012) Biomaterials, 33, 4241-4250.
14) Ikemoto, S., Mochizuki, M., Yamada, M., Takeda, A., Uchinuma, E., Yamashina, S., Nomizu, M., &
Kadoya, Y. (2006) J. Biomed. Mater. Res. A, 79, 716-722.
15) Masuda, R., Mochizuki, M., Hozumi, K., Takeda, A., Uchinuma, E., Yamashina, S., Nomizu, M., &
Kadoya, Y. (2009) Wound Repair Regen., 17, 127-135.
2
2
1 2
Spatiotemporal resolution of the Ntla transcriptome in axial mesoderm development.
Shestopalov IA, Pitt CL, Chen JK., Nat. Chem. Biol., 8, 270–276(2012).
(MO)
(cMO) MO
( .1(a))
Ntla cMO
Ntla ( .1(b))
(cFD) cMO
Ntla
Ntla
Ntla 100
Ntla 8
Ntla
Ntla
Ntla 2 flh fgf8a
Ntla Ntla
Spatiotemporal control of gene expression by a light-switchable transgene system.
Xue Wang, Xianjun Chen, Yi Yang, Nat. Methods., 9 , 266–269 (2012).
400 nm
1 (a) (cMO) . (b) cMO (cFD)
. ( )
Vivid Gal4 ( .2(a))
2 1
2 100
LED Insulin
( .2(b))
Light-based feedback for controlling intracellular signaling dynamics.
Toettcher JE, Gong D, Lim WA, Weiner OD. Nat. Methods., 8 , 837–839 (2011).
2 (a) Vivid . (b)
. mCherry . mCherry
, mCherry . (
)
Vivid
PI3K PhyB PIF6
650 nm 750 nm
750 nm S/N
2 PI3K PI3K
PhyB PIF6 PI3K
PI3K (PIP2)
(PIP3) PI3K
PIP3 Akt
PIP3 .3(a),
(b) PIP3 Akt PIP3
.3(c)
PI3K PI3K
400 PI3K LY294002
PI3K
3 (a) PhyB PIF6 PI3K . PHAkt PIP3 Akt .
(b) . (c)
(With feedback( )) (No feedback( )) . (
)
400 800
400 800
400 800
T (sec)
LY294002(PI3K )
Target function Light(650 nm) ight(650 nm)ight(650 nm)
PIF6 PhyB
in vivo
DNA
Asymmetry and aging of mycobacterial cells lead to variable growth and antibiotic susceptibility B. B. Aldridge, M. Fernandez-Suarez, D. Heller, V. Ambravaneswaran, D. Irimia, M. Toner, S. M. Fortune, Science, 355, 100–104 (2012).
150 HIV/
Mycobacterium tuberculsis
M. tuberculsis M.
smegmatis
1
10-17 µm 0.8-0.9 µm 2
Rapid, sensitive, and quantitative detection of pathogenic DNA at the point of care through microfluidic electrochemical quantitative loop-mediated isothermal amplification
K. Hsieh, A. S. Patterson, B. S. Ferguson, K. W. Plazco, H. T. Soh, Angew. Chem. Int. Ed., 51, 4896–4900 (2012).
(POC)
POC Hsieh
Salmonella enterica enterica MEQ-LAMP
MB LAMP
DNA MB
DNA DNA MB LAMP
DNA MB DNA
2 MB
LAMP
PCR 4 fg/µL
DNA POC
Human gut-on-a-chip inhabited by microbial flora that experiences intestinal peristalsis-like motions and flow
H. J. Kim, D. Hub, G. Hamilton D. E. Ingber, Lab Chip, DOI: 10.1039/C2LC40074J (2012).
gut-on-a-chip 10 µm
50%
3 Caco-2 BBe 3
21 3
30-40 µm 6
3e
3-4 7
Lactobacillus rhamnosus GG (LGG) LGG
(Max Planck Institute of Molecular Physiology)
Herbert Waldmann 2011 3
4 Waldmann
Waldmann 1 2010 3
Waldmann Waldmann
2 Waldmann 2
Waldmann fellowship
NRW 59
30
Das Bitte! (
!)
( 50 !!)
8
( )
2010-2011 2011-2012
(40 m)
2
Waldmann
PI Waldmann
10
60
EU PhD
MPI MPI
Waldmann
-
SAR Study Pull Down Probe
PhD 30
MPI
Andrey
Herbert Waldmann Andrey Antonchick
第６回バイオ関連化学シンポジウム
（第 27 回生体機能関連化学シンポジウム、第 15 回バイオテクノロジー部会シンポジウ ム、第 15 回生命化学研究会シンポジウム）
http://jointsympo.csj.jp/
2012 9 6 8
17 8
18 10
JR 25
–
15 5 3 1 1
2 2
1 40
WEB http://jointsympo.csj.jp/
2012 6 19 ( )
2012 7 13 ( )
2012 7 23 ( )
7 23 5,000 3,000
7,000 4,000
7 24 … 2,000
* *
2012 9 7 ( ) 6,000
6
001-0021 21 10
TEL: 011-706-9360 FAX: 011-706-9361 E-Mail [email protected]
第 39 回国際核酸化学シンポジウム
The 39
thInternational Symposium on Nucleic Acids Chemistry (ISNAC2012)
http://www.knt.co.jp/ec/2012/ISNAC2012/1. Chemistry of Nucleosides, Nucleotides, and Their Analogues 2. Medicinal Chemistry of Nucleosides and Oligonucleotides 3. DNA/RNA Chemistry and Biochemistry
4. DNA/RNA Structure and Recognition 5. Ribozymes, siRNAs, and miRNAs 6. DNA/RNA Materials and Diagnostics
7. Drug Delivery Systems and Nanotechnology of Oligonucleotides
2012 11 15 11 17
URL
2012 8 12
2012 9 2
URL
2012 10 15
25,000 30,000
5,000 7,000
464-8603
TEL: 052-789-2488 FAX: 052-789-2528 E-Mail [email protected]
http://www.knt.co.jp/ec/2012/ISNAC2012/
5,000
アンチセンス・遺伝子・デリバリーシンポジウム 2012 http://res.tagen.tohoku.ac.jp/~antisens/index.html
2012 9 24 26
4 1
DNA/RNA
4'-
RNA
RNA
Dectin-1 DDS
3,000 4,000 5,000 6,000
2
9 25 5,000 2,000
2012 7 31
15
2012 8 31
2012
980-8577 2 1 1 203 TEL
& FAX 022-217-5633 e-mail [email protected]
http://res.tagen.tohoku.ac.jp/~antisens/index.html
第 2 回アジアケミカルバイオロジー会議（第 15 回 生命化学研究会）
The Second Asian Chemical Biology Conference (ACBC2) http://www.chem.sci.osaka-u.ac.jp/lab/fukase/acbc2
2012 7 4 7 6 3
901-0305 1-6-1
10 km 20
. . . . .
HP
HP
http://www.chem.sci.osaka-u.ac.jp/lab/fukase/acbc2
5 15 40,000 45,000 20,000 ;
5 16 45,000 50,000 25,000
: 560-0043 1-1
ACBC2 Tel: 06-6850-5388 Fax: 06-6850-5419
HP: http://www.chem.sci.osaka-u.ac.jp/lab/fukase/acbc2 E–mail: [email protected]
24
2012 9 19 61
520-2194 1-5
Tel: 077-543-7469 Fax: 077-543-7483 E-mail: [email protected]
680-8552 4-101
Tel: 0857-31-5262
E-mail: [email protected]
224-8501 4259-B55
Tel: 045-924-5844 Fax: 045-924-5806 E-mail: [email protected]
860-8555 2-39-1
Tel & Fax: 096-342-3872 Fax: 096-342-3679 E-mail: [email protected]
