希少ウマ科動物の生息域外保全に向けた繁殖生理に関する内分泌学的研究

Title 希少ウマ科動物の生息域外保全に向けた繁殖生理に関する_{内分泌学的研究( 本文(Fulltext) )} Author(s) MUREN Report No.(Doctoral Degree) 博士(農学) 甲第633号 Issue Date 2014-09-24 Type 博士論文 Version ETD URL http://hdl.handle.net/20.500.12099/50390 ※この資料の著作権は、各資料の著者・学協会・出版社等に帰属します。



ᕼ

ᕼᑡ࣐࢘⛉ື≀ࡢ⏕ᜥᇦእಖ඲࡟ྥࡅࡓ

⦾Ṫ⏕⌮࡟㛵ࡍࡿෆศἪᏛⓗ◊✲

2014 ᖺ

ᒱ㜧኱Ꮫ኱Ꮫ㝔㐃ྜ㎰Ꮫ◊✲⛉

⏕≀⏕⏘⛉Ꮫ

㸦ᒱ㜧኱Ꮫ㸧

MUREN

ii

ᕼ

ᕼᑡ࣐࢘⛉ື≀ࡢ⏕ᜥᇦእಖ඲࡟ྥࡅࡓ

⦾Ṫ⏕⌮࡟㛵ࡍࡿෆศἪᏛⓗ◊✲

i

┠

┠㻌 㻌 ḟ

ᗎ

ᗎ㻌 ㄽ㻌

䞉䞉䞉䞉䞉䞉䞉䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䞉䞉䞉䞉䞉䞉䞉 1 ᪥ᮏᅾ᮶㤿䛾ಖ඲⌧≧䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡 2 㔝⏕ᕼᑡ䜴䝬⛉ື≀䛾⌧≧䛸⏕ᜥᇦእಖ඲⦾Ṫ䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡 3 ᐙ␆䜴䝬䛾⦾Ṫ䛻㛵䜟䜛䝩䝹䝰䞁䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡 4 ᛶ䝇䝔䝻䜲䝗䝩䝹䝰䞁䛾௦ㅰ䛚䜘䜃᤼ἥ䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡 5 ⣅୰䝩䝹䝰䞁 ᐃ䛻䜘䜛㠀౵くⓗෆศἪ䝰䝙䝍䝸䞁䜾䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡 6 ◊✲䛾┠ⓗ䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡

➨

➨䊠❶㻌 ᮌ᭮㤿䛻䛚䛡䜛⣅୰䛾ᛶ䝇䝔䝻䜲䝗䝩䝹䝰䞁 ᐃ䛾᭷⏝ᛶ䛾

᳨ウ䛸Ⓨ᝟࿘ᮇ䛚䜘䜃ዷፎᮇ䛾䝩䝹䝰䞁ືែ

䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡 1㸫1 ㅖゝ䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡 1㸫2 ᮦᩱ䛚䜘䜃᪉ἲ䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡 1㸫2㸫1 ౪ヨື≀䛸䝃䞁䝥䝹᥇ྲྀ䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡 1㸫2㸫2 ⾑୰䛾䝥䝻䝆䜵䝇䝔䝻䞁ྵ㔞䛾 ᐃ䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡㻌 1㸫2㸫3 ⣅୰䛾ᛶ䝇䝔䝻䜲䝗䝩䝹䝰䞁௦ㅰ≀䛾ᢳฟ䛸 ᐃ䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡㻌 1㸫2㸫4 㓝⣲ච␿ ᐃἲ䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡㻌 1㸫2㸫5 㓝⣲ච␿ ᐃἲ䛾ᡭ㡰䛸䝩䝹䝰䞁⃰ᗘ䛾ィ⟬䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡㻌 1㸫3㻌 䝕䞊䝍ศᯒ䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡㻌 1㸫4 ⤖ᯝ䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡 1㸫4㸫1 ⣅୰䛾ᛶ䝇䝔䝻䜲䝗䝩䝹䝰䞁௦ㅰ≀ྵ㔞䛾⤒᫬ⓗኚ໬䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡㻌 1㸫4㸫2 ᮌ᭮㤿䛾⣅୰䝥䝻䝆䜵䝇䝔䝻䞁ྵ㔞䛾ືែ䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡㻌 1㸫5 ⪃ᐹ䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡㻌 ➨䊠❶䛾ᅗ⾲䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡㻌

➨

➨䊡❶㻌 ᕼᑡ䜴䝬⛉㻟✀䛻䛚䛡䜛⣅୰䛾ᛶ䝇䝔䝻䜲䝗䝩䝹䝰䞁䛾㛗ᮇ㻌

1 1 1 6 10 12 12 15 15 16 16 17 17 18 20 20 27 27 27 28 31

ii

ⓗ

ⓗ䝰䝙䝍䝸䞁䜾

䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡

㻌㻌

2䠉1 ㅖゝ䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡㻌 2䠉2 ᮦᩱ䛚䜘䜃᪉ἲ䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡 2䠉2䠉1㻌 ౪ヨື≀䞉⣅䛾᥇ྲྀ䛸ᐃ㔞䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡 2䠉2䠉2 እぢኚ໬䛸ᛶ⾜ື䛾ほᐹ䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡㻌 2䠉3㻌 䝕䞊䝍ศᯒ䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡㻌 2䠉4 ⤖ᯝ䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡㻌 2䠉4䠉1 䝰䜴䝁䝜䜴䝬䛾⣅୰䛾ᛶ䝇䝔䝻䜲䝗䝩䝹䝰䞁௦ㅰ≀ྵ㔞䛾ືែ䡡䡡䡡䡡䡡䡡䡡䡡㻌 2䠉4䠉2 䝋䝬䝸䝜䝻䝞䛾⣅୰䛾ᛶ䝇䝔䝻䜲䝗䝩䝹䝰䞁௦ㅰ≀ྵ㔞䛾ືែ䡡䡡䡡䡡䡡䡡䡡䡡 2䠉4䠉3 䜾䝺䝡䞊䝅䝬䜴䝬䛾⣅୰䛾ᛶ䝇䝔䝻䜲䝗䝩䝹䝰䞁௦ㅰ≀ྵ㔞䛾ືែ䡡䡡䡡 2䠉5 ⪃ᐹ䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡 ➨䊡❶䛾ᅗ⾲䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡

➨

➨䊢❶㻌 ᕼᑡ䜴䝬⛉ື≀䛾ዷፎᮇ䛻䛚䛡䜛⣅୰䛾ᛶ䝇䝔䝻䜲䝗䝩䝹䝰㻌㻌 㻌 㻌 㻌 㻌 㻌

㻌 㻌 㻌 㻌 䞁௦ㅰ≀䛾ᐃᛶ

䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡

㻌㻌

3䠉1㻌 ㅖゝ䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡 3䠉2㻌 ᮦᩱ䛚䜘䜃᪉ἲ䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡 3䠉2䠉1 ౪ヨື≀䛸ศᯒᮦᩱ䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡㻌 3䠉2䠉2 ⣅୰䛾ᛶ䝇䝔䝻䜲䝗䝩䝹䝰䞁௦ㅰ≀䛾ᢳฟ䛸ᢳฟᾮ䛾⢭〇䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡㻌 3䠉2䠉3 㧗㏿ᾮయ䜽䝻䝬䝖䜾䝷䝣䜱䞊䛾タᐃ᮲௳䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡㻌 3䠉2䠉4 ⣅୰ᛶ䝇䝔䝻䜲䝗䝩䝹䝰䞁௦ㅰ≀䛾ศ㞳䛸䜶䞊䝔䝹ᢳฟ䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡㻌 3䠉2䠉5 ᛶ䝇䝔䝻䜲䝗䝩䝹䝰䞁௦ㅰ≀䛾ྠᐃ䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡 3䠉3 ⤖ᯝ䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡 3䠉4 ⪃ᐹ䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡㻌 ➨3 ❶䛾ᅗ⾲䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡

⥲

⥲ᣓ

䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡㻌 37 37 38 38 39 39 43 43 44 44 45 51 61 61 62 62 63 64 64 65 68 68 71 79

iii

ⱥ

ⱥᩥせ⣙

䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡㻌

ㅰ

ㅰ㎡

䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡

ᘬ

ᘬ⏝ᩥ⊩

䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡䡡㻌 㻌 㻌 83 86 88

1

ᗎ

ᗎ ㄽ

1 ᪥ᮏᅾ᮶㤿ࡢ⌧≧ ᪥ᮏ࡛ࡣ㸪໭ᾏ㐨࿴✀㤿㸪ᮌ᭮㤿㸪㔝㛫㤿㸪ᑐᕞ㤿㸪ᚚᓮ㤿㸪ࢺ࢝ࣛ㤿㸪ᐑྂ㤿㸪 ୚㑣ᅜ㤿ࡢ8 ✀ࡀᅾ᮶㤿࡜ࡋ࡚ㄆࡵࡽࢀ࡚࠸ࡿ㸦ᒸ㒊, 1953㸧ࠋ᪥ᮏᅾ᮶㤿ࡣ㸪ࡍ࡭࡚ ᑠᆺ㹼୰ᆺ㤿࡛࠶ࡾ㸪ࣔࣥࢦࣝࡢ௚㸪୰ᅜࡸᮅ㩭༙ᓥ࡛᭱ࡶ୍⯡ⓗ࡛࠶ࡗࡓⵚྂ㤿⣔ ࡟ᒓࡍࡿ㸦㔝⃝, 1999㸧ࠋࡑࡢ୰࡛ᮌ᭮㤿࡟ࡘ࠸࡚ࡢ᭱ྂࡢグ㘓ࡣ㸪࿴₎୕ᡯᅗ఍ࡢࠕ➨ ஧୐௦Ᏻ㛩ኳⓚ஧ᖺ㸪㤿ࢆ⛉㔝ࡢᅜ࡟ᨺࡗ࡜ᮃ᭶∾㸪㟝ཎࡢ∾࡟ᨺࡕ࠸ࡉࡉ࠿࡟ᚋୡ ∵㤿࡟ஈࡋ࠿ࡽࡎ࡜ࠖ࡜࠸࠺グ㏙࡛࠶ࡿ㸦ఀ⸨, 1996㸧ࠋ㙊಴᫬௦࡟ධࡿ࡜ᮌ᭮ࡣྡ㤿 ࡢ⏘ᆅ࡜ࡋ࡚ྡኌࡀ㧗ࡲࡾ㸪ㅖᅜࡢṊᑗࡀ➇ࡗ࡚ඃ㥴ࢆồࡵࡓ࡜࠸ࢃࢀ࡚࠸ࡿࠋࡲࡓ ᫂἞᫬௦࡟ࡣ㸪㌷஦ᣑᙇ஦ᴗࡢࡓࡵᅾ᮶㤿ࡢᨵⰋࡀ㛤ጞࡉࢀ㸪᫂἞39 ᖺ࡟ࡣ࿴✀ࡀ⣙ 129 ୓㢌࡛࠶ࡗࡓࡢ࡟ᑐࡋ㞧✀࠾ࡼࡧὒ✀ࡣ⣙ 18 ୓㢌࡛࠶ࡗࡓࡀ㸪᫛࿴ 7 ᖺ࡟ࡣ㏫࡟ ๓⪅ࡀ⣙5 ୓㢌࡛࠶ࡗࡓࡢ࡟ᑐࡋ࡚ᚋ⪅ࡀ 142 ୓㢌࡜࡞ࡗࡓ㸦ᒸ㒊, 1953㸧ࠋࡉࡽ࡟㸪 ᫛࿴ 14 ᖺ࡟✀㤿⤫ไἲࡀ᪋⾜ࡉࢀ㸪࿴✀㞝㤿ࡣᮏἲᚊࡢᑐ㇟እ࡟࡞ࡗࡓࡓࡵ㸪ࡑࢀ ௨㝆ࡢ᪥ᮏᅾ᮶㤿ࡀᚭᗏⓗ࡞ᾘ⁛ࡢ༴ᶵ࡟ℜࡋࡓ㸦ᒸ㒊, 1953㸧ࠋࡋ࠿ࡋ㸪ᅾ᮶㤿ࡢ✀ ∻ࡣ㸪ឡዲ⪅ࡢᡭඖࡸൢᆅ࡛ࡣᩘᑡ࡞ࡃ㣫⫱ࡉࢀ࡚࠸ࡓ㸦ᒸ㒊, 1953㸧ࠋࡇࢀࡽࡢᅾ᮶ 㤿ࡣ㸪ᛶ㉁ 㡰࡛ᣢஂຊ࡟ᐩࡳ㣫㣴⟶⌮࡞࡝ࡶẚ㍑ⓗᐜ࡛᫆࠶ࡿ≉㉁ࢆಖ᭷ࡋࡓ᪥ᮏ ᅛ᭷ࡢ㈗㔜࡞㑇ఏ㈨※࡜ᩥ໬㑇⏘࡛࠶ࡿ㸦᪥ᮏ㤿஦༠఍, 2009㸧ࠋࡇࡢࡓࡵ㸪࡯࡜ࢇ࡝ ࡢ✀࡛ࡣ᫛࿴50 ᖺ๓ᚋ࡟ಖᏑ఍ࢆタ❧ࡋ㸪✚ᴟⓗ࡞ಖᏑάືࡀ㐍ࡵࡽࢀ࡚࠸ࡿࠋ㎰⪔ ࡸ㍺㏦ࡢᶵᲔ໬࡟ࡼࡗ࡚㸪ᅾ᮶㤿ࡢ㎰⪔㤿࡜ࡋ࡚ࡢᙺ๭ࡀ࡞ࡃ࡞ࡗࡓ⌧௦♫఍࡛ࡣక ౶ື≀࡜ࡋ࡚㣫⫱ࡉࢀ࡚࠸ࡿࠋࡋ࠿ࡋ㸪ࡇࢀࡽࡢ✀ࡀಖ඲ࡉࢀ࡚࠸ࡿᆅᇦఫẸࡢ㧗㱋 ໬ࡀ㐍ࡴࡇ࡜ࡸ࣐࢘ࡢデᐹࡀ࡛ࡁࡿ⋇་ᖌࡀᑡ࡞࠸ࡇ࡜࡟ࡼࡗ࡚㸪⤯⁛ࡢ༴㝤ᛶࡶ㠀 ᖖ࡟㧗࠸࡜ࡉࢀ࡚࠸ࡿࠋ 2 㔝⏕ᕼᑡ࣐࢘⛉ື≀ࡢ⌧≧࡜⏕ᜥᇦእಖ඲⦾Ṫ

2

㔝⏕࣐࢘⛉ື≀ࡣୡ⏺࡛1 ᒓ 5 ளᒓ 7 ✀ࡀ⌧Ꮡࡋ࡚࠾ࡾ㸦♽㇂, 1984㸧㸪ᅜ㝿⮬↛ಖ

ㆤ㐃ྜ㸦International Union for Conservation of Nature and Natural Resources; IUCN㸧ࡢ ࣞࢵࢻࣜࢫࢺ㸦The IUCN Red List of Threatened Species; ᅗ 1㸧࡟ࡼࡿ࡜㸪࢔ࣇࣜ࢝ࣀ ࣟࣂ㸦Equus afrianus㸧ࡣ⤯⁛༴᝹ IA 㢮㸦IUCN, 2014a㸧㸪ࣔ࢘ࢥࣀ࣐࢘㸦E. przewalskii㸧㸪

ࢢࣞࣅ࣮ࢩ࣐࣐࢘㸦E. grevyi㸧࠾ࡼࡧ࢔ࢪ࢔ࣀࣟࣂ㸦E. hemionus㸧ࡣྛ䚻⤯⁛༴᝹ IB

㢮㸦IUCN, 2014b, c, d㸧㸪࣐ࣖࢩ࣐࣐࢘㸦E. zebra㸧ࡣ⤯⁛༴᝹ II 㢮㸦IUCN, 2014e㸧࡟

ᣦᐃࡉࢀ࡚࠸ࡿࠋࡲࡓ㸪ࢧࣂࣥࢼࢩ࣐࣐࢘㸦E. quagga㸧࡜ࢳ࣋ࢵࢺࣀࣟࣂ㸦E. kiang㸧 ࡟ࡘ࠸࡚ࡣపᗘᠱᛕ㸦LC㸧࡛࠶ࡿ࡜ᣦ᦬ࡉࢀ࡚࠸ࡿ㸦IUCN, 2014f, g㸧ࠋ㔝⏕࣐࢘⛉ື ≀ࡢῶᑡࡢ୺࡞ཎᅉࡣ㸪⓶ࡸ㣗⫗ࡢࡓࡵࡢ஘⋓㸪㝈ࡽࢀࡓỈ࣭㣵㈨※ࡢࡓࡵࡢᐙ␆࡜ ࡢ➇த㸪㐣ᨺ∾࡟ࡼࡿ⎔ቃࡢᝏ໬㸪ᐙ␆࠿ࡽࡢ␿⑓ ఏ᧛࡞࡝ࡀ⪃࠼ࡽࢀ࡚࠸ࡿ 㸦Moehlman, 2002㸧ࠋࡲࡓ㸪ࡇࢀࡽࡢ✀ࡢ࡯࡜ࢇ࡝ࡣ࢔ࣇࣜ࢝ࡸ࢔ࢪ࢔ࡢ༙ᖸࡤࡘᆅᇦ ࡟⏕ᜥࡋ࡚࠸ࡿࡇ࡜࠿ࡽ㸪㛗ᮇⓗ࡛ཝࡋ࠸஝⇱᮲௳ୗ࡛ࡢ⥅⥆ⓗ࡛㐺ษ࡞⟶⌮ࢆ⾜ࢃ ࡞ࡃ࡚ࡣ࡞ࡽࡎ㸪ࡑࢀࢆᐇ⌧ࡍࡿࡓࡵࡢ ༑ศ࡞㈨㔠࡜ேᮦࡀ᥼ຓࡉࢀ࡚࠸࡞࠸ 㸦Moehlman, 2002㸧ࠋᚑࡗ࡚㸪ࡇࢀࡽࡢ㔝⏕ᕼᑡ࣐࢘⛉ື≀✀ࡢಶయᩘࡀ௒ᚋࡶ⥅⥆ⓗ ࡟ῶᑡࡍࡿྍ⬟ᛶࡀ⪃࠼ࡽࢀ࡚࠾ࡾ㸪ື≀ᅬ࡛ࡢ⏕ᜥᇦእಖ඲ࡢࡓࡵࡢ⦾Ṫࢆ㔜どࡍ ࡿ࡞࡝ࡢከࡃࡢᑐ⟇ࢆㅮࡎࡿᚲせࡀ࠶ࡿࠋ 1960 ᖺ௦࠿ࡽ㔝⏕⏕≀ಖ඲ࡣከࡃࡢື≀ᅬ᪋タ࡟࡜ࡗ࡚኱ࡁ࡞౑࿨࡜࡞ࡾ㸦World Association of Zoos and Aquariums; WAZA, 2006㸧㸪⤯⁛ࡢ༴ᶵ࡟ℜࡋ࡚࠸ࡿ㔝⏕ື≀ࢆ 㣫⫱ୗ࡛⦾Ṫࡉࡏ㸪㑇ఏⓗከᵝᛶࢆಖࡕ࡞ࡀࡽ㛗ᮇ࡟ࢃࡓࡗ࡚✀ࢆ⥔ᣢࡍࡿࡇ࡜ࡀồ

ࡵࡽࢀ࡚࠸ࡿࠋ1993 ᖺ࡟ୡ⏺ື≀ᅬಖ඲ᡓ␎ࡀ⟇ᐃࡉࢀ㸪ୡ⏺ࡢື≀ᅬࡀ✀ࡢಖ඲ࢆ

⾜࠺ᶵ㛵࡬࡜㐍໬ࡍࡿ᪉ྥᛶࡀ♧ࡉࢀ㸦Nowell and Jackson, 1996; WAZA, 2006㸧㸪ື≀

ᅬࡀᆅ⌫つᶍࡢಖ඲άືࢆ⾜࠺ࡓࡵࡢ୍ဨ࡜ࡋ࡚㈉⊩ࡍ࡭ࡁᙺ๭ࡀᥦゝࡉࢀࡓࠋ

౛࠼ࡤ㸪ࣔ࢘ࢥࣀ࣐࢘ࡣ 1877 ࡟࣮ࣚࣟࢵࣃே࡟ࡼࡗ࡚Ⓨぢࡉࢀࡓࡀ㸪ࡑࡢᚋ 100

ᖺࡶ⤒ࡎ㔝⏕ୗ࡛ࡣ⤯⁛ࡋࡓ㸦WAZA, 2006㸧ࠋࡋ࠿ࡋᖾ࠸࡞ࡇ࡜࡟㸪ື≀ᅬ࡛ࡣ 1899

3 ࡍࡿ࡟ࡍࡂ࡞࠿ࡗࡓ㸦♽㇂, 1984㸧ࠋࡑࡢᚋࡢ 1959 ᖺ 9 ᭶࡟➨୍ᅇࡢࣔ࢘ࢥࣀ࣐࢘ಖㆤ ᅜ㝿ࢩ࣏ࣥࢪ࣒࢘ࡀࣉࣛࣁ࡛㛤࠿ࢀ㸪ື≀ᅬ࡛ࡢቑṪ࡟㛵ࡋࡓ᳨ウ఍㆟ࡀ㛤࠿ࢀࡓ㸦♽ ㇂, 1984; WAZA, 2006㸧ࠋࡲࡓ㸪WAZA ࡢ㈶ຓࡢࡶ࡜ࣉࣛࣁື≀ᅬ࡛ 1960 ᖺ௨㝆࡟ẖ ᖺࣔ࢘ࢥࣀ࣐࢘ࡢᅜ㝿⾑⤫Ⓩ㘓ྎᖒࡀⓎ⾜ࡉࢀࡿࡼ࠺࡟࡞ࡗࡓ㸦♽㇂, 1984; WAZA, 2006㸧ࠋࡇࢀࡽࡢࡇ࡜ࡀࡑࡢᚋࡢ㣫⫱ୗࣔ࢘ࢥࣀ࣐࢘࡟ᑐࡍࡿಖ඲⦾ṪάືࡀάⓎ࡟⾜ ࢃࢀࡿዎᶵ࡜࡞ࡾ㸪1980 ᖺ௦୰ᮇࡲ࡛࡟㣫⫱ୗ࡛ 1000 㢌௨ୖࡲ࡛ᅇ᚟ࡋࡓ㸦WAZA, 2006; IUCN, 2013b㸧ࠋࡉࡽ࡟㸪1990 ᖺ࡟ࢻ࢖ࢶ࡟ᣐⅬࢆ⨨ࡃࢡࣜࢫࢳ࣭ࣕࣥ࢜ࢬ࣡ࣝ ࢻᇶ㔠࡟ࡼࡗ࡚㸪ࣔࣥࢦ࡛ࣝ㔝⏕᚟ᖐࣉࣟࢪ࢙ࢡࢺࡀ㛤ጞࡉࢀࡓ⤖ᯝ㸪⌧ᅾ㔝⏕⎔ቃ ࡟㐺ᛂࡋࡓಶయ⩌ࡀ㡰ㄪ࡟ቑ࠼࡚࠸ࡿ(WAZA, 2006; IUCN, 2013b)ࠋࡇࢀࡣ㣫⫱ୗ⦾Ṫ ࡟ࡼࡗ࡚✀ࡢಖ඲࡜㔝⏕ᅇ᚟ࡀᡂຌࡋࡓ୍ࡘࡢ௦⾲౛࡜࡞ࡾ㸪௚ࡢᕼᑡ✀ಖ඲⦾Ṫ࡟ ࡶ኱࠸࡟ཧ⪃࡟࡞ࡿࡶࡢ࡛࠶ࡿࠋ ୍᪉㸪ື≀ᅬ࡟࠾ࡅࡿᕼᑡ࣐࢘⛉ື≀ࡢ⦾Ṫࡣ࡯࡜ࢇ࡝⮬↛⦾Ṫ࡛࠶ࡿࠋ⮬↛⦾Ṫ ࢆ⾜࠺ሙྜ࡟ࡣ㸪ࡇࢀࡽࡢ✀ࡢ㔝⏕≧ἣୗ࡛ࡢ⏕άᵝᘧ࡜⦾Ṫ᝟ሗࡢ཰㞟ࡀ㔜せ࡜࡞ ࡿࡀ㸪ື≀ᅬ࡟࠾ࡅࡿ㣫⫱⎔ቃ㸦᪥↷᫬㛫㸪Ẽ ࠾ࡼࡧᰤ㣴᮲௳࡞࡝㸧࡟ࡼࡗ࡚ᛶᡂ ⇍᫬ᮇࡸ⦾ṪᏘ⠇࡞࡝࡟ࡶᙳ㡪ࢆ୚࠼ࡿࡓࡵ㸪㣫⫱ୗಶయ࡟࠾ࡅࡿ⦾Ṫ࡟㛵ࡍࡿᇶ♏ ⓗ࡞▱ぢࡢゎ᫂ࡀồࡵࡽࢀ࡚࠸ࡿࠋࡉࡽ࡟㸪ṇ☜࡞ዷፎุᐃ࡜ฟ⏘᫬ᮇࡢண ࡣಶయ ࡢ㣫⫱ୗ࡛ࡢ⟶⌮࡜ḟࡢ⦾Ṫィ⏬࡟୙ྍḞ࡞᝟ሗ࡟࡞ࡿࡓࡵ㸪ዷፎࡢྍ⬟ᛶࡀ࠶ࡿಶ య࡟ࡘ࠸࡚ࡢෆศἪᏛⓗ࡞ࣔࢽࢱࣜࣥࢢࡀᚲせ࡛࠶ࡿࠋ୍᪉࡛㸪㏆ᖺ㣫⫱ୗ࡛ࡢ㝈ࡽ ࢀࡓಶయࡢ㑇ఏⓗከᵝᛶ࡟㓄៖ࡋ࡚㸪㣫⫱ࢫ࣮࣌ࢫࡢၥ㢟ࡸ㍺㏦࡟࠿࠿ࡿࣜࢫࢡࢆ㑊 ࡅࡿࡓࡵ㸪ᐙ␆ࡢ⏕⏘⌧ሙ࡛㢖⦾࡟ᛂ⏝ࡉࢀ࡚࠸ࡿேᕤ⦾Ṫᢏ⾡ࢆᕼᑡ࣐࢘⛉ື≀࡛ ☜❧࡛ࡁࡿࡼ࠺࡟ດຊࡉࢀ࡚࠸ࡿ㸦Adams et al., 2009㸧ࠋ౛࠼ࡤ㸪ࣔ࢘ࢥࣀ࣐࡛࢘ࡣ㟁

Ẽ่⃭࡟ࡼࡿ᥇⢭㸦Collins et al., 2006㸧㸪ࢢࣞࣅ࣮ࢩ࣐࣐࢘࡜ࢢࣛࣥࢺࢩ࣐࣐࢘㸦E. q.

boehmi㸧࡛ࡣேᕤ⭼ἲ࡛⢭Ꮚࡢ᥇㞟ࢆ⾜࠸ಖᏑࡶ࡛ࡁࡿ⌧ἣ࡟࠶ࡿ㸦Crump and Crump,

1994㸧ࠋࣔ࢘ࢥࣀ࣐࢘࡜ࢢࣛࣥࢺࢩ࣐࣐࡛࢘ࡣ⬇ࡢ᥇ྲྀࡶྍ⬟࡜࡞ࡗ࡚࠾ࡾ㸪⬇ࢆ㏆⦕ ✀ ࡛ ࠶ ࡿ ᐙ ␆ ࢘ ࣐ ࡜ ࣟ ࣂ ࡟ ⛣ ᳜ ࡉ ࡏ ࡿ ࡇ ࡜ ࡟ ࡼ ࡾ ௦ ⌮ ฟ ⏘ ࡟ ࡶ ᡂ ຌ ࡋ ࡚ ࠸ ࡿ

4

㸦Summers et al., 1987㸧ࠋࡲࡓ㸪ࡇࢀࡽࡢᢏ⾡࡟ࡼࡗ࡚ᚓࡽࢀࡓ⢭Ꮚࡲࡓࡣ⬇ࢆຠ⋡ⓗ ࡟ேᕤ⦾Ṫ࡟⏝࠸ࡿࡓࡵ࡟ࡣ㸪ᕼᑡ࣐࢘⛉ື≀✀ࡢ⦾Ṫ⏕⌮≧ἣࡢ⥅⥆ⓗ࡞ࣔࢽࢱࣜ ࣥࢢ࡟ࡼࡿ⦾ṪෆศἪᏛⓗ࡞≉ᛶࢆゎ᫂ࡍࡿࡇ࡜ࡀࡶࡗ࡜ࡶ㔜せ࡟࡞ࡿࡶࡢ࡜⪃࠼ࡽ ࢀ࡚࠸ࡿࠋ

5 ࢝ࢸࢦ࣮ࣜ ᇶ ‽ ⤯⁛㸦EX㸧  ࡍ࡛࡟⤯⁛ࡋࡓ࡜⪃࠼ࡽࢀࡿ✀ 㔝⏕⤯⁛㸦EW㸧 㣫⫱࣭᱂ᇵୗ࡛࠶ࡿ࠸ࡣ㐣ཤࡢศᕸᇦእ࡟ಶయ㸦ಶయ⩌㸧ࡀᖐ໬ ࡋ࡚⏕ᜥࡋ࡚࠸ࡿ≧ែࡢࡳ⏕Ꮡࡋ࡚࠸ࡿ✀ ⤯⁛༴᝹I 㢮 ⤯⁛ࡢ༴ᶵ࡟ℜࡋ࡚࠸ࡿ✀ࠋ⌧ᅾࡢ≧ែࢆࡶࡓࡽࡋࡓᅽ㏕せᅉࡀ ᘬࡁ⥆ࡁస⏝ࡍࡿሙྜ㸪㔝⏕࡛Ꮡ⥆ࡀᅔ㞴࡞ࡶࡢ IA 㢮㸦CR㸧 ࡈࡃ㏆࠸ᑗ᮶࡟࠾ࡅࡿ㔝⏕࡛ࡢ⤯⁛ࡢ༴㝤ᛶࡀᴟࡵ࡚㧗࠸ࡶࡢ IB 㢮㸦EN㸧 IA 㢮࡯࡝࡛ࡣ࡞࠸ࡀ㸪㏆࠸ᑗ᮶࡟࠾ࡅࡿ㔝⏕࡛ࡢ⤯⁛ࡢ༴㝤ᛶࡀ 㧗࠸ࡶࡢ ⤯⁛༴᝹ϩ㢮㸦VU㸧 ⤯⁛ࡢ༴㝤ࡀቑ኱ࡋ࡚࠸ࡿ✀ࠋ⌧ᅾࡢ≧ែࢆࡶࡓࡽࡋࡓᅽ㏕せᅉ ࡀᘬࡁ⥆࠸࡚స⏝ࡍࡿሙྜ㸪㏆࠸ᑗ᮶ࠕ⤯⁛༴᝹I 㢮ࠖࡢࣛࣥࢡ ࡟⛣⾜ࡍࡿࡇ࡜ࡀ☜ᐇ࡜⪃࠼ࡽࢀࡿࡶࡢ ㍍ᗘᠱᛕ㸦LC) ᇶ‽࡟↷ࡽࡋ㸪ୖグࡢ࠸ࡎࢀ࡟ࡶヱᙜࡋ࡞࠸✀ࠋศᕸࡀᗈ࠸ࡶࡢ ࡸ㸪ಶయᩘࡢከ࠸✀࡛࠶ࡿࡶࡢ ᝟ሗ୙㊊㸦DD㸧 ࢹ࣮ࢱ୙㊊ࡢࡓࡵุᐃࡉࢀ࡚࠸࡞࠸ࡶࡢ

ᅗ1  2013 IUCN Red List of Threatened Species ࡢ࢝ࢸࢦ࣮ࣜ࡜ᇶ‽㸦IUCN ᪥ᮏጤဨ఍

6 3 ᐙᐙ␆࣐࢘ࡢ⦾Ṫ࡟㛵ࢃࡿ࣍ࣝࣔࣥ ࣐࢘ࡣ඾ᆺⓗ࡞㛗᪥⦾Ṫື≀࡛࠶ࡾ㸪໭༙⌫࡛ࡣ 5㹼10 ᭶ࡀ⦾ṪᏘ⠇࡛࠶ࡿ 㸦England, 2005㸧ࠋᏘ⠇⦾Ṫື≀࡛ࡣ㸪║࡛ཷᐜࡉࢀࡓගࡢ᝟ሗࡣどᗋୗ㒊ࡢど஺ཫୖ ᰾㸦SCN㸧ࢆ௓ࡋ࡚ᯇᯝయ࡟ఏࢃࡾ㸪ࡑࡇ࠿ࡽኪ㛫࡟ศἪࡉࢀࡿ࣓ࣛࢺࢽࣥࡢศἪࣃ ࢱ࣮ࣥ࡟ࡼࡾ᪥㛗᝟ሗࡀఏ㐩ࡉࢀࡿ㸦ụୖ࡜ྜྷᮧ, 2010㸧ࠋ㛗᪥⦾Ṫື≀࡛ࡣᬯᮇࡀ▷ ࡃ࡞ࡿ࡜࣓ࣛࢺࢽࣥࡢศἪ㔞ࡀῶᑡࡋ㸪ᛶ⭢่⃭࣍ࣝࣔࣥᨺฟ࣍ࣝࣔࣥ㸦gonadotropin

releasing hormone: GnRH㸧ࡢቑຍࢆᘬࡁ㉳ࡇࡍ㸦Ginther, 1993; ụୖ࡜ྜྷᮧ, 2010㸧ࠋࡑ

ࡋ࡚GnRH ࡢᏘ⠇ⓗ࡞ኚ໬࡟ᛂࡌ࡚㯤యᙧᡂ࣍ࣝࣔࣥ㸦Luteinizing hormone : LH㸧ࡸ

༸⬊่⃭࣍ࣝࣔࣥ㸦Follicle stimulating hormone : FSH㸧ࡀቑῶࡋ㸪ᛶ⭢ࡢⓎ㐩ࡀไᚚࡉ

ࢀࡿ㸦Ginther, 1993㸧ࠋࡲࡓ㸪ᛶ⭢࠿ࡽศἪࡉࢀࡿᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ࡟ࡼࡗ࡚⏕Ṫ ᶵ⬟࡞࡝ࡢ኱ษ࡞⏕࿨άືࡀᨭ㓄ࡉࢀ࡚࠸ࡿࠋ ᐙ␆࡛࠶ࡿ࣐࢘ࡢⓎ᝟࿘ᮇࡣ19㹼26 ᪥㛫࡛࠶ࡾ㸪ከࡃࡣ 20㹼24 ᪥㛫㸦ᖹᆒ 23 ᪥ 㛫㸧ࡢ࿘ᮇ࡛Ⓨ᝟ࢆ⧞ࡾ㏉ࡍ㸦኱⃝, 2006㸧ࠋⓎ᝟࿘ᮇࡢ㛗ࡉࢆỴࡵࡿࡢࡣⓎ᝟ᮇࡢ㛗 ࡉ࡛࠶ࡾ㸪Ⓨ᝟ᮇ㛫ࡣ5㹼7 ᪥㛫⥔ᣢࡍࡿ㸦኱⃝, 2006㸧ࠋⓎ᝟ࡣᰤ㣴≧ែࡢⰋ࠸㞤㤿࡛ ࡣ▷ࡃ㸪ᝏ࠸ࡶࡢ࡛ࡣ㛗ࡃ࡞ࡾ㸪ࡲࡓⓎ᝟ࢆ㛤ጞࡍࡿᏘ⠇ࡢⓎ᝟ᮇ㛫ࡣ㛗ࡃ࡞ࡿഴྥ ࡟࠶ࡿ㸦኱⃝㸪2006㸧ࠋⓎ᝟ᮇࡢึࡵ࡟ࡣ⾑୰࡟༸⬊࠿ࡽศἪࡉࢀࡿ࢚ࢫࢺࣟࢪ࢙ࣥࡀ 㧗࠸್ࢆ♧ࡍࡀ㸪ࣉࣟࢪ࢙ࢫࢸࣟࣥ⃰ᗘࡣప࠸ࠋ୺ᖍ༸⬊ࡣⓎ⫱ࢆ⥆ࡅ࡞ࡀࡽ㸪ࡉࡽ ࡟ከࡃࡢ࢚ࢫࢺࣟࢪ࢙ࣥࢆศἪࡍࡿࠋ࢚ࢫࢺࣟࢪ࢙ࣥࡢ㈇ࡢࣇ࢕࣮ࢻࣂࢵࢡ࡟ࡼࡾ㸪 GnRH ࡟ᑐࡋ࡚ᢚไⓗ࡟స⏝ࡋ࡚ࣃࣝࢫ≧ศἪࢆ⥅⥆ࡋ࡚༸⬊ࢆᡂ⇍ࡉࡏࡓᚋࡣ㸪ṇ ࡢࣇ࢕࣮ࢻࣂࢵࢡ࡟ࡼࡾಁ㐍ⓗ࡟స⏝ࡋ࡚ୗᆶయࢆ่⃭ࡋLH ࢧ࣮ࢪࡀㄏ㉳ࡉࢀࡿࠋ ࡇࡢLH ࡣⓎ᝟ᮇࢆ㏻ࡋ࡚ᚎࠎ࡟⃰ᗘࡀቑຍࡋ㸪᤼༸㸦Ⓨ᝟⾜ືࡀ⤊஢ࡍࡿ 24 ᫬㛫๓ ࡟㉳ࡇࡿ㸧ࡢ⩣᪥࠿ࡽ⩣ࠎ᪥࡟࠿ࡅ࡚ࣆ࣮ࢡࢆ♧ࡍࠋ⾑୰࢚ࢫࢺࣟࢪ࢙ࣥ⃰ᗘࡣ᤼༸ ᚋ1㹼2 ᪥࡟࠿ࡅ࡚ᇶᗏ್ࡲ࡛ῶᑡࡍࡿࡀ㸪ࣉࣟࢪ࢙ࢫࢸ್ࣟࣥࡣ᤼༸ᚋ 5 ᪥๓ᚋ㸦4 㹼7 ᪥㸧࡟ࣆ࣮ࢡࢆ♧ࡍ㸦኱⃝, 2006㸧ࠋཷ⫾ࡋ࡚࠸࡞࠸ሙྜ࡟ࡣ㸪᤼༸ࡢ⣙ 2 㐌㛫ᚋ ࡟ࡣᏊᐑࡼࡾศἪࡉࢀࡿ PGF2Ș㸦Prostaglandin F2Ș㸧࡟ࡼࡾ㯤య㏥⾜ࡀጞࡲࡾ㸪⾑୰ࣉ

7 ࣟࢪ࢙ࢫࢸࣟࣥ⃰ᗘࡀῶᑡࡍࡿ 㸦England, 2005; ኱⃝, 2006㸧ࠋࡲࡓ㯤యᮇ୰ᮇ࡟༸⬊ Ⓨ⫱࡟㔜せ࡞FSH ࢧ࣮ࢪࡀㄆࡵࡽࢀ࡚࠸ࡿ㸦ᅗ 2㸪Ginther, 1993; ኱⃝, 2006㸧ࠋ ᐙ␆ࡢ࣐࢘ࡢዷፎึᮇࡢࣉࣟࢪ࢙ࢫࢸࣟࣥ㸪࢚ࢫࢺࣟࢪ࢙ࣥ࠾ࡼࡧࢸࢫࢺࢫࢸࣟࣥ ࡣ༸ᕢ࠿ࡽศἪࡉࢀࡿࡀ㸪ዷፎ⣙60 ᪥┠௨㝆࡟⫾┙࠿ࡽศἪࡉࢀࡿ⾑୰ࣉࣟࢪ࢙ࢫࢸ ࣟࣥ௦ㅰ≀㸦5Ș-ࣉࣞࢢࢼࣥ㸧ࡢቑຍࡀጞࡲࡾ㸪ฟ⏘ࡢ 1 ࣨ᭶๓࠿ࡽᛴ⃭࡟ቑຍࡍࡿ 㸦ᅗ3㸪Ginther, 1993; ὠ᭤, 2006㸧ࠋࡲࡓ㸪࢚ࢫࢺࣟࢪ࢙ࣥ࡜ࢸࢫࢺࢫࢸࣟࣥࡀዷፎ୰ ᮇ࡟ࣆ࣮ࢡ್ࢆ♧ࡍࡀ㸪ᚋᮇ࡟࠿ࡅ࡚పୗࡍࡿࠋࡇࡢ࢚ࢫࢺࣟࢪ࢙ࣥ࡜ࢸࢫࢺࢫࢸࣟ ࣥࡣ⫾Ꮚ࣭⫾┙ࣘࢽࢵࢺ࡟ࡼࡗ࡚ศἪࡉࢀ㸪Ꮚᐑ࠾ࡼࡧ⫾┙ࡢ⾑ὶ㔞ࢆቑຍࡉࡏ⫾Ꮚ ࡢⓎ⫱ࢆಁ㐍ࡍࡿᙺ๭ࡀ࠶ࡿࡶࡢ࡜᥎ᐹࡉࢀ࡚࠸ࡿࠋ

8

ᅗ2  ᐙ␆࣐࢘ࡢⓎ᝟࿘ᮇ୰࡟࠾ࡅࡿ⾑୰ࡢྛ✀࣍ࣝࣔࣥࡢືែ

9

ᅗ3  ᐙ␆࣐࢘ࡢዷፎᮇ㛫୰࡟࠾ࡅࡿ⾑୰ࡢྛ✀࣍ࣝࣔࣥࡢືែ

10 4 ᛶᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥࡢ௦ㅰ࠾ࡼࡧ᤼ἥ ⾑୰ࡢᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥࡣ㸪࡯࡜ࢇ࡝ࡑࡢࡲࡲࡢᙧ࡛⣅୰࡟᤼ἥࡉࢀࡎ㸪⫢⮚ ࡢ௦ㅰ࡟ࡼࡗ࡚༑ᩘ✀㢮ࡢ௦ㅰ≀࡜ࡋ࡚⣅୰࡟᤼ἥࡉࢀࡿࡇ࡜ࡀ▱ࡽࢀ࡚࠸ࡿ 㸦Schwarzenberger, 2007㸧ࠋ౛࠼ࡤ㸪P4 ࡣ⫢⮚࡟࠾࠸࡚୺࡟㑏ඖ཯ᛂ࡟ࡼࡾ㸪5Ș ࡲࡓ ࡣ5ș 㑏ඖᆺࣉࣞࢢࢼࣥ㸦ࣉࣞࢢࢼࣥࢪ࢜ࣥ㸪ࣔࣀࣄࢻࣟ࢟ࢩࣉࣞࢢࢼࣥ࠾ࡼࡧࢪࣄ ࢻࣟ࢟ࢩࣉࣞࢢࢼࣥ㸧࡟௦ㅰࡉࢀ㸪ࡲࡎ㸪ࢫࢸࣟ࢖ࢻ㦵᱁ࡢC4㸫C5 ఩㛫ࡢ஧㔜⤖ྜ ࡢ㑏ඖ࡟ࡼࡾࣉࣞࢢࢼࣥࢪ࢜ࣥ࡜࡞ࡾ㸪ḟ࡟ C3 ఩ࡢ㑏ඖ཯ᛂ࡟ࡼࡾࣔࣀࣄࢻࣟ࢟ࢩ ࣉࣞࢢࢼࣥ㸦ࣉࣞࢢࢼࣀࣟࣥ㸧࡟࡞ࡿࠋࡉࡽ࡟C20 ఩ࡢ㑏ඖ཯ᛂ࡟ࡼࡾࢪࣄࢻࣟ࢟ࢩ ࣉࣞࢢࢼࣥ㸦ࣉࣞࢢࢼࣥࢪ࣮࢜ࣝ㸧࡬࡜㌿᥮ࡉࢀࡿ㸦ᅗ4㸧ࠋ ࣐࢘⛉ື≀ࡢ⦾ṪෆศἪືែࡣ௚ࡢື≀✀࡜ࡣ␗࡞ࡾ㸪ዷፎᮇࡢᐙ␆ࡢ࣐࢘࡟࠾࠸ ࡚ࡣዷፎ๓ᮇࡢዷፎ㯤య࡟ࡼࡗ࡚ศἪࡉࢀࡿ㧗⃰ᗘࡢࣉࣟࢪ࢙ࢫࢸࣟࣥ࡜17α-ࣄࢻࣟ ࢟ࢩࣉࣟࢪ࢙ࢫࢸࣟࣥ㸦OHP㸧ࡀ⾑୰᳨࡛ฟࡉࢀ㸪ࡲࡓ୰࣭ᚋᮇ࡟ࡣ⫾┙࠿ࡽศἪࡉ ࢀࡿ㧗⃰ᗘࡢ 5α-ࣉࣞࢢࢼࣥ࡜࢚ࢫࢺࣟࢪ࢙ࣥࡀ᳨ฟࡉࢀࡿࡇ࡜ࡶྠᵝ࡟ሗ࿌ࡉࢀ࡚ ࠸ࡿ㸦Ginther, 1993㸧ࠋࡇࢀࡽࡢ࣍ࣝࣔࣥࡣ୺࡟⫢⮚࡛௦ㅰࡉࢀ࡚࠿ࡽయእ࡟᤼ἥࡉࢀ ࡿࡓࡵ㸪ዷፎࡢྛ᫬ᮇ࡟ࡼࡾ⣅୰ࡢᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ௦ㅰ≀ࡀ␗࡞ࡿྍ⬟ᛶࡀ⪃ ࠼ࡽࢀ㸪ࡉࡽ࡟᤼ἥࡉࢀࡿ௦ㅰ≀ࡀື≀✀࡟ࡼࡗ࡚␗࡞ࡿࡇ࡜ࡶሗ࿌ࡉࢀ࡚࠸ࡿ 㸦Schwarzenberger, 2007㸧ࠋࡇࢀࡽࡢࡇ࡜࠿ࡽ㸪࣐࢘⛉ᕼᑡ✀࡟࠾ࡅࡿዷፎᮇࡢ⦾Ṫෆ ศἪᏛⓗ࡞≉ᛶࢆヲ⣽࡟ゎ᫂ࡍࡿ࡟ࡣ㸪ዷፎࡢྛ᫬ᮇ࡟࠾ࡅࡿ⣅୰ࡢᛶࢫࢸࣟ࢖ࢻ࣍ ࣝࣔࣥ௦ㅰ≀ࡢ✀㢮ࢆヲ⣽࡟ᐃᛶศᯒࡍࡿᚲせࡀ࠶ࡿࠋ

୍᪉㸪ࢩࣂࣖࢠ㸦Capra hircus㸧㸪ࣈࢱ࢜ࢨࣝ㸦Macaca nemestrina㸧㸪࢘ࢩ㸦Bos taurus㸧㸪

࣐࢘࠾ࡼࡧࣈࢱ㸦Sus scrofa domesticus㸧ࡢ⣅୰ࡢࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ௦ㅰ≀ྵ㔞ࡣ㸪

᤼ἥᚋࡢ30 ศ㛫㹼48 ᫬㛫ࡢ㛫࡟᭷ព࡞ቑῶࡀㄆࡵࡽࢀ㸦Wasser et al., 1988; Hirata and

Mori, 1995; Möstl et al., 1999㸧㸪⣅୰ࡢ⣽⳦ࡸ㓝⣲ࡢస⏝࡟ࡼࡗ࡚ࡇࢀࡽࡢ௦ㅰ≀ྵ㔞

ࡀ⤒᫬ⓗ࡟ኚ໬ࡍࡿࡇ࡜ࡶሗ࿌ࡉࢀ࡚࠸ࡿ㸦Kharn et al., 2002㸧ࠋᚑࡗ࡚㸪EIA ἲ࡞࡝

11 ࢖ࢻ࣍ࣝࣔࣥࡢᢠయ࡟஺ཫ཯ᛂࢆ♧ࡍᵓ㐀ୖ㢮ఝࡋࡓᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ௦ㅰ≀ࢆ ྠ᫬࡟ᤊ࠼ࡓ್࡛࠶ࡿ࡜⪃࠼ࡽࢀࡿࠋ ᅗ4 ࣉࣟࢪ࢙ࢫࢸࣟࣥࡢ௦ㅰ㸦⋢⯋㸪1999 ࡼࡾ㌿㍕࣭⦅㞟) ձ 㸸ࣉࣞࢢࢼࣥࢪ࢜ࣥ㸪ղ㸸ࣔࣀࣄࢻࣟ࢟ࢩࣉࣞࢢࢼࣥ㸪ճ㸸ࢪࣄࢻࣟ࢟ࢩࣉࣞࢢ ࢼࣥ

12 5 ⣅⣅୰࣍ࣝࣔࣥ ᐃ࡟ࡼࡿ㠀౵くⓗෆศἪࣔࢽࢱࣜࣥࢢ ື≀ࡢ⦾Ṫ⏕⌮ࡢࣔࢽࢱࣜࣥࢢ࡟ࡣ㸪⾑୰ࡢᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ⃰ᗘࡢ ᐃࢆ⾜ ࠺ࡇ࡜ࡀ୍⯡ⓗ࡛࠶ࡿࠋࡋ࠿ࡋ㸪ᐃᮇⓗ࠿ࡘ㢖⦾࡞᥇⾑ࡣື≀࡟ᑐࡋ࡚ࢫࢺࣞࢫࢆ୚ ࠼ࡿࡤ࠿ࡾ࡛ࡣ࡞ࡃ㸪᥇⾑⪅࡟ࡶᑡ࡞࠿ࡽࡎ༴㝤ࡀక࠺ࠋ≉࡟㔝⏕ࡢ࣐࢘⛉ື≀࡛ࡣ㸪 ᤕ⋓ࡸಖᐃࡑࡢࡶࡢࡀᅔ㞴࡛࠶ࡿࠋ㏆ᖺ㸪㠀౵くⓗ࡞᪉ἲ࡛࠶ࡿ⣅୰ࡢᛶࢫࢸࣟ࢖ࢻ ࣍ࣝࣔࣥ௦ㅰ≀ྵ㔞ࡢ ᐃἲࡀὀ┠ࡉࢀ㸪ࡇࡢ᪉ἲࡀᵝࠎ࡞ື≀✀࡛ෆศἪᏛⓗ࡞ࣔ ࢽࢱࣜࣥࢢ࡟౑ࢃࢀ࡚࠸ࡿ㸦Schwarzenberger, 2007㸧ࠋᐙ␆࣐࢘✀ࡢࢧࣛࣈࣞࢵࢻ

㸦Schwarzenberger et al., 1991; Palme et al., 2001; Janowski et al., 2008㸧㸪࢔ࣛࣈ㸦Janowski

et al., 2008㸧㸪࣏ࢽ࣮㸦Palme et al., 1997; Palme et al., 2001㸧㸪ࣜࣆࢵࢶ࢓ࢼ࣮

㸦Schwarzenberger et al., 1991㸧㸪ࢺࣟࢵࢱ࣮㸦Schwarzenberger et al., 1991㸧࡜㔝⏕࣐࢘ ⛉ᕼᑡ✀ࡢࣔ࢘ࢥࣀ࣐࢘㸦Schwarzenberger et al., 1992㸧㸪ࢢࣞࣅ࣮ࢩ࣐࣐࢘㸦Asa et al., 2001㸧㸪ࢳࣕࢵࣉ࣐ࣥࢩ࣐࣐࢘㸦E㸬quagga chapmani㸧㸦Ncube et al., 2011㸧࡞࡝ࡢ✀࡛ ࡶ㸪⣅୰ࡢᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ௦ㅰ≀ྵ㔞ࡢ ᐃ࡟ࡼࡾ༸ᕢᶵ⬟ࡸዷፎᮇࡢ⦾Ṫ⏕ ⌮ࢆࣔࢽࢱࣜࣥࢢࡋࡓሗ࿌ࡀ࡞ࡉࢀ࡚࠸ࡿࠋࡇࡢࡼ࠺࡟㸪ᮏἲࡀ࣐࢘⛉ື≀ࡢᇶ♏ⓗ ࡞⦾ṪෆศἪ⌧㇟ࢆ◊✲ࡍࡿࡓࡵࡢᡭἲ࡜ࡋ࡚᭷ຠ࡛࠶ࡿࡇ࡜ࡀ᫂ࡽ࠿࡟࡞ࡗ࡚࠸ࡿࠋ ୍⯡࡟㸪⣅୰ࡢᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥྵ㔞ࡢ ᐃἲࡣ⾑୰࣍ࣝࣔࣥ⃰ᗘࡢ᳨ᰝ᪉ἲ ࡟ẚ࡭࡚ศᯒ㐣⛬ࡀ↹㞧࡛࠶ࡿࡇ࡜ࡸ㸪ࢹ࣮ࢱࡢゎ㔘ࡀᅔ㞴࡞ሙྜࡶ࠶ࡿ㸦ᴋ⏣ࡽ, 2006㸧ࠋࡋ࠿ࡋ㸪ࢧࣥࣉࣝ᥇ྲྀࡢᐜ᫆ࡉ࠿ࡽ㢖⦾࡟᥇ྲྀࡍࡿࡇ࡜ࡀྍ⬟࡛࠶ࡿࡓࡵ㸪ࡼ ࡾヲ⣽࡞࣍ࣝࣔࣥືែࢆㄪ࡭ࡿࡇ࡜ࡀྍ⬟࡜࡞ࡿࠋ 6 ◊✲ࡢ┠ⓗ ᕼᑡ࡞࣐࢘⛉ື≀✀ࡢ⦾Ṫ⏕⌮࡟㛵ࡋࡓሗ࿌౛ࡣ࡜࡚ࡶᑡ࡞ࡃ㸪⏕ᜥᇦእ࡛ࡢ⦾Ṫ ࡢಁ㐍࡟㔜せ࡞᝟ሗ࡜࡞ࡿᕼᑡ✀ࡢ⦾Ṫ⏕⌮࡟ࡘ࠸࡚ࡣ࠸ࡲࡔ࡟୙᫂࡞Ⅼࡀከࡃṧࡉ ࢀ࡚࠸ࡿࡓࡵ㸪௒ᚋࡢゎ᫂ࡀᮇᚅࡉࢀ࡚࠸ࡿࠋື≀ࡢ⦾Ṫ⏕⌮ࡢゎ᫂࡟ࡣ㸪⾑୰ࡢᛶ ࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ⃰ᗘࡢ ᐃࡀ୍⯡ⓗ࡞᪉ἲ࡜࡞ࡗ࡚࠸ࡿࠋࡋ࠿ࡋ㸪࣐࢘⛉ື≀࡟

13 ࠾ࡅࡿ⥅⥆ⓗ࡞᥇⾑ࡣᅔ㞴࡛࠶ࡿࡓࡵ㸪⣅ࢆ⏝࠸ࡓᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ௦ㅰ≀ྵ㔞 ࡢ ᐃἲࡣ㸪⦾ṪෆศἪ≧ἣࡢᢕᥱ࡟ᗈࡃ⏝࠸ࡽࢀࡿࡼ࠺࡟࡞ࡗ࡚ࡁࡓࠋᮏ◊✲࡛ࡣ㸪 ᮌ᭮㤿㸪ࣔ࢘ࢥࣀ࣐࢘㸪ࢯ࣐ࣜࣀࣟࣂ࠾ࡼࡧࢢࣞࣅ࣮ࢩ࣐࣐࢘㸦ᅗ 5㸧ࡢ⣅୰ࡢᛶࢫ ࢸࣟ࢖ࢻ࣍ࣝࣔࣥ௦ㅰ≀ྵ㔞ࡢ ᐃ࡟ࡼࡾ㸪ྛࠎࡢ✀ࡢ⦾Ṫ⏕⌮ࡢ≉ᚩࢆゎᯒࡋ㸪ࡇ ࢀࡽࡢ✀ࡢ⏕ᜥᇦእಖ඲࡟᭷⏝࡞⦾ṪෆศἪᏛⓗ▱ぢࢆ᫂ࡽ࠿࡟ࡍࡿࡇ࡜ࢆ┠ⓗ࡜ࡋ ࡓࠋ ➨Ϩ❶࡛ࡣ㸪࣐࢘⛉ື≀ࡢ⣅୰ࡢᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ௦ㅰ≀ྵ㔞࡟࠾ࡅࡿ᤼ἥᚋ ࡢ⤒㐣᫬㛫࡟క࠺ኚ໬࡟ࡘ࠸࡚᫂ࡽ࠿࡟ࡍࡿࡓࡵ㸪㠀ዷፎᮇࡢᮌ᭮㤿ࡢ┤⭠⣅ࢆ᥇ྲྀ ࡋ࡚࠿ࡽ20Υୗ࡛ಖ ࡋ㸪᥇ྲྀ┤ᚋ࠿ࡽ 168 ᫬㛫ࡲ࡛ࡢ⣅୰ࡢᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ ௦ㅰ≀ྵ㔞ࡢኚ໬࡟ࡘ࠸࡚ㄪ࡭ࡓࠋࡲࡓ㸪⾑୰࡜⣅୰ࣉࣟࢪ࢙ࢫࢸ್ࣟࣥࡢືែࢆẚ ㍑ࡋ㸪⣅ヨᩱࢆ⏝࠸ࡓ ᐃἲࡀᮌ᭮㤿ࡢ⦾Ṫ⏕⌮ࡢゎ᫂࡟᭷⏝࠿࡝࠺࠿࡟ࡘ࠸᳨࡚ウ ࡋࡓࠋࡇࢀࡽࡢ▱ぢࢆࡶ࡜࡟㸪ᮌ᭮㤿ࡢ⦾ṪෆศἪᏛⓗ࡞≉ᚩࢆ᫂ࡽ࠿࡟ࡍࡿࡓࡵ࡟㸪 ⣅୰ࡢᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥྵ㔞ࢆ㛗ᮇ㛫࡟ࢃࡓࡗ࡚ ᐃࡋࡓࠋ ➨ϩ❶࡛ࡣ㸪࣐࢘⛉ᕼᑡ✀࡛࠶ࡿࣔ࢘ࢥࣀ࣐࢘㸪ࢯ࣐ࣜࣀࣟࣂ࠾ࡼࡧࢢࣞࣅ࣮ࢩ࣐ ࣐࢘ࡢ⦾ṪෆศἪᏛⓗ࡞▱ぢࢆヲ⣽࡟᫂ࡽ࠿࡟ࡍࡿࡓࡵ࡟㸪㛗ᮇ㛫࡟ࢃࡓࡗ࡚ྠࡌಶ యࡢ⣅୰ࡢᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥྵ㔞ࡢືែࢆࣔࢽࢱࣜࣥࢢࡋࡓࠋ ➨Ϫ❶࡛ࡣ㸪࣐࢘⛉ᕼᑡ✀ࡢዷፎᮇ୰ࡢྛ᫬ᮇ࡟࠾ࡅࡿ⣅୰ࡢᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔ ࣥ௦ㅰ≀ࡢ✀㢮ࢆ᫂ࡽ࠿࡟ࡋࡼ࠺࡜ࡋࡓࠋࡍ࡞ࢃࡕ㸪ዷፎᮇࢆ౽ᐅⓗ࡟๓ᮇ㸪୰ᮇ㸪 ᚋᮇࡢ3 ᮇ࡟ศࡅ㸪ྛࠎࡢ᫬ᮇࡢ௦ㅰ≀ࢆ HPLC ࡟ࡼࡾศ㞳ࢆ⾜ࡗࡓࠋᚓࡽࢀࡓྛࣇ ࣛࢡࢩࣙࣥࡢ⃰ᗘࢆEIA ἲ࡛ ᐃࡋ࡚㸪ࡇࢀࡽࡢ⃰ᗘ࡜ᶆ‽≀㉁ࡢ⁐ฟ᫬㛫ࢆẚ㍑ࡍ ࡿࡇ࡜࡟ࡼࡾ㸪࣐࢘⛉ᕼᑡ✀ࡢ⣅୰࡟᤼ἥࡉࢀࡿᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ௦ㅰ≀ࢆྠᐃ ࡋࡓࠋ

14

ᅗ5  ᮏ◊✲࡛౪ヨࡋࡓ 4 ✀ࡢ࣐࢘⛉ᕼᑡື≀

㸦1㸧ᮌ᭮㤿㸦Equus caballus㸧㸪㸦2㸧ࣔ࢘ࢥࣀ࣐࢘㸦E. przewalskii㸧㸪㸦3㸧ࢯ࣐ࣜ

15 ➨ ➨Ϩ❶ ᮌ᭮㤿࡟࠾ࡅࡿ⣅୰ࡢᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ ᐃࡢ᭷⏝ᛶࡢ᳨ウ࡜Ⓨ᝟࿘ ᮇ࠾ࡼࡧዷፎᮇࡢ࣍ࣝࣔࣥືែ 1㸫1 ㅖ ゝ ᮌ᭮㤿ࡣ㛗㔝┴ᮌ᭮㒆࠾ࡼࡧᒱ㜧┴ᮾ⃰ᆅ᪉ࢆ୰ᚰ࡟㣫㣴ࡉࢀ࡚࠸ࡿ᪥ᮏᅾ᮶㤿ࡢ ᕼᑡရ✀࡛࠶ࡿ㸦⏣୰, 2008㸧ࠋ⌧௦♫఍࡛ࡣ㸪㎰⪔ࡢᶵᲔ໬ࡸ㍺㏦ࡢ㏆௦໬࡟ࡼࡗ࡚㸪 ᮌ᭮㤿ࡢ㎰⪔㤿࡜ࡋ࡚ࡢᙺ๭ࡀ࡞ࡃ࡞ࡾ㸪኱ṇ᫬௦ࡢ᭱┒ᮇ࡟ࡣ 6,800 㢌ࢆ㉸࠼ࡿ࡯ ࡝㣫㣴ࡉࢀ࡚࠸ࡓᮌ᭮㤿ࡣ1976 ᖺ࡟ࡣ 32 㢌࡟ࡲ࡛ῶᑡࡋ㸪⤯⁛ࡢ༴ᶵ࡟┤㠃ࡋࡓ㸦ఀ ⸨, 1996㸧ࠋࡑࡢࡓࡵ 1983 ᖺ࡟ࡣᮌ᭮㤿ࡀ㛗㔝┴ࡢኳ↛グᛕ≀࡟ᣦᐃࡉࢀ㸪ᮌ᭮㤿ࡢಖ ඲ᇶ┙ࡀᩚഛࡉࢀጞࡵ㸪ࡑࡢᚋ㣫㣴㢌ᩘࡀᚎࠎ࡟ቑຍࡋࡓ㸦⏣୰, 2008㸧ࠋ⌧ᅾ㸪Ⓩ㘓 㢌ᩘࡀ158 㢌ࡲ࡛ᅇ᚟ࡋࡓࡀ㸦ᮌ᭮㤿ಖᏑ఍஦ົᒁ, 2013㸧㸪౫↛࡜ࡋ࡚⤯⁛ࡢ༴ᶵࢆ ⬺ࡋࡓ࡜ࡣ࠸࠼ࡎ㸪ᮏ✀ࡢ㣫㣴ࡢ⥔ᣢ࠾ࡼࡧ⦾Ṫࡢ᥎㐍ࡀồࡵࡽࢀ࡚࠸ࡿࠋࡑࡢࡓࡵ㸪 ྛಶయࡢ⦾Ṫ࡟㛵ࢃࡿ⏕⌮≧ែࢆヲ⣽࡟ᢕᥱࡋ࡚࠾ࡃࡶ㔜せ࡞せ⣲࡜࡞ࡿࠋ ື≀ࡢ⦾Ṫ⏕⌮ࡢࣔࢽࢱࣜࣥࢢ࡟ࡣ㸪⾑୰ࡢᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ⃰ᗘࡢ ᐃࡀ୍ ⯡ⓗ࡞᪉ἲ࡛࠶ࡿࠋࡋ࠿ࡋ㸪ᐃᮇⓗ࠿ࡘ㢖⦾࡞᥇⾑ࡣᑐ㇟㤿࡟ᑐࡋ࡚ᗄศࡢࢫࢺࣞࢫ ࢆ୚࠼ࡿࡤ࠿ࡾ࠿᥇⾑⪅࡟ࡶᑡ࡞࠿ࡽࡎ༴㝤ࡀక࠺ࠋ㏆ᖺ㸪㠀౵くⓗ࡞᪉ἲ࡛࠶ࡿ⣅ ୰ࡢᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ௦ㅰ≀ྵ㔞ࡢ ᐃἲࡀ㸪≉࡟ື≀ᅬື≀ࡸ㔝⏕ື≀ࡢ⦾Ṫ ศ㔝࡛ὀ┠ࡉࢀ㸪⾑୰ࡢᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥࡢศἪືែࢆᢕᥱࡍࡿࡓࡵࡢ௦᭰ἲ࡜ ࡋ࡚ᵝࠎ࡞ື≀✀࡛౑ࢃࢀ࡚࠸ࡿ㸦Schwarzenberger, 2007㸧ࠋࡲࡓ㸪ᐙ␆ࡢ࣏ࢽ࣮㸦Equus caballus㸧࡛ࡣ㸪⾑୰࡟ᢞ୚ࡋࡓ14C-ࣉࣟࢪ࢙ࢫࢸࣟࣥ㸦14C-P4㸧ࡢ75%ࡀ⣅୰࡟᤼ἥ ࡉࢀࡓࡇ࡜ࡀ᫂ࡽ࠿࡟ࡉࢀ㸦Palme et al., 1996㸧㸪ࣔ࢘ࢥࣀ࣐࡛࢘ࡣ㸪⣅୰ P4௦ㅰ≀ྵ 㔞 ࡢ   ᐃ ࡟ ࡼ ࡾ 㯤 య ά ື ࡢ ࣔ ࢽ ࢱ ࣜ ࣥ ࢢ ࡀ ྍ ⬟ ࡛ ࠶ ࡿ ࡇ ࡜ ࡀ ሗ ࿌ ࡉ ࢀ ࡚ ࠸ ࡿ 㸦Schwarzenberger et al., 1992㸧ࠋࡉࡽ࡟㸪ࢢࣞࣅ࣮ࢩ࣐࣐࡛࢘ࡣ㸪⣅୰ P4௦ㅰ≀ྵ㔞ࡸ ࢚ࢫࢺࣟࢪ࢙ࣥྵ㔞ࡢ ᐃࡣ༸ᕢ࿘ᮇ࠾ࡼࡧዷፎᮇࡢࣔࢽࢱࣜࣥࢢ࡟᭷ຠ࡛࠶ࡿࡇ࡜ ࡀሗ࿌ࡉࢀ࡚࠸ࡿ㸦Asa et al., 2001㸧ࠋࡋ࠿ࡋ㸪ࡇࢀࡲ࡛࡟ᮌ᭮㤿࡟ࡘ࠸࡚ࡣ㸪ᙧែᏛ

16

ⓗ࡞≉ᚩࡸ㑇ఏᙧ㉁࡞࡝࡟㛵ࡍࡿሗ࿌ࡣ࠶ࡿࡀ㸦Takasu et al., 2011, 2012㸧㸪⦾Ṫ⏕⌮ ࡟㛵ࡍࡿሗ࿌ࡣぢᙜࡓࡽ࡞࠸ࠋ

⣅୰ࡢࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ௦ㅰ≀ྵ㔞ࡣ㸪᤼ἥ┤ᚋ࠿ࡽ⤒᫬ⓗ࡟ኚ໬ࡍࡿࡇ࡜ࡀ▱ ࡽࢀ࡚࠸ࡿ㸦Wasser et al., 1988; Möstl et al., 1999; Kharn et al., 2002㸧ࠋࢩࣂࣖࢠ㸦Capra

hircus㸧ࡢ⣅୰ P4௦ㅰ≀ྵ㔞ࡣ㸪᤼ἥ࠿ࡽ30 ศᚋ࡟ࡣ᭷ព࡟㧗࠸್ࢆ♧ࡋ㸪ࡑࢀ௨㝆

࡟ୖ᪼ഴྥࡀㄆࡵࡽࢀ࡚࠸ࡿ㸦Hirata and Mori, 1995㸧ࠋࡲࡓ㸪ࣈࢱ࢜ࢨࣝ㸦Macaca

nemestrina㸧ࡢ⣅୰ P4௦ㅰ≀ྵ㔞ࡣ㸪᤼ἥ࠿ࡽ 6 ᫬㛫ᚋ࡟᭷ព࡟㧗࠸್ࢆ♧ࡋࡓࡢ࡟

ᑐࡋ㸪E2ྵ㔞ࡣ᭷ព࡟ప࠸್ࢆ♧ࡋࡓ㸦Wasser et al., 1988㸧ࠋࡉࡽ࡟㸪࢘ࢩ㸦Bos taurus㸧㸪

࣐࢘㸦E. caballus㸧࠾ࡼࡧࣈࢱ㸦Sus scrofa domesticus㸧ࡢ⣅୰ࢥࣝࢳࢰ࣮ࣝ௦ㅰ≀ࡀࡑ

ࢀࡒࢀ᤼ἥ࠿ࡽ 1 ᫬㛫ᚋ㸪4 ᫬㛫ᚋ࠾ࡼࡧ 24 ᫬㛫ᚋ࡟᭷ព࡞ቑຍࡀㄆࡵࡽࢀ࡚࠸ࡿ 㸦Möstl et al., 1999㸧ࠋࡋࡓࡀࡗ࡚㸪⣅୰ࡢᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥྵ㔞ࡢືែ࠿ࡽື≀ ࡢ⏕⌮Ꮫⓗ࡞≧ἣࢆṇ☜࡟ホ౯ࡍࡿࡓࡵ࡟ࡣ㸪⣅ࡢ᤼ἥ࠿ࡽ᥇ྲྀࡲࡓࡣಖᏑࡲ࡛ࡢ㐺 ษ࡞᫬㛫᮲௳࡟ࡘ࠸࡚Ỵᐃࡋ࡞ࡅࢀࡤ࡞ࡽ࡞࠸ࠋ ᮏ◊✲࡛ࡣ㸪ࡲࡎ⣅ࡢ㐺ษ࡞ศᯒ᮲௳ࢆ☜ㄆࡍࡿࡓࡵ㸪ᮌ᭮㤿ࢆࣔࢹࣝ࡜ࡋ࡚┤⭠ ⣅ࢆ᥇ྲྀࡋ20Υୗ࡛ಖᏑࡋ࡚㸪ࡑࡢ⤒㐣᫬㛫࡟ࡼࡿ⣅୰ࡢᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ௦ㅰ ≀ྵ㔞ࡢኚ໬࡟ࡘ࠸࡚ㄪ࡭ࡓ㸦ᐇ㦂 1㸧ࠋḟ࡟㸪⾑୰࡜⣅୰ P4್ࡢືែࢆẚ㍑ࡋ㸪⣅ ࢆ⏝࠸ࡓ ᐃࡢ᭷⏝ᛶ࡟ࡘ࠸᳨࡚ウࡋࡓࠋࡲࡓ㸪⣅ࢆ⏝࠸ࡓ ᐃ࡟ࡼࡾ㸪ᮌ᭮㤿ࡢ༸ ᕢ࿘ᮇ㸦Ⓨ᝟࿘ᮇ㸧ࡸ⦾ṪᏘ⠇㸪ዷፎࡢෆศἪືែࢆ᫂ࡽ࠿࡟ࡋࡼ࠺࡜ࡋࡓ㸦ᐇ㦂2㸧ࠋ 1㸫㸫2 ᮦᩱ࠾ࡼࡧ᪉ἲ 1㸫2㸫1 ౪ヨື≀࡜ࢧࣥࣉࣝ᥇ྲྀ ᒱ㜧኱Ꮫᛂ⏝⏕≀⛉Ꮫ㒊㝃ᒓᒱ㜧ࣇ࢕࣮ࣝࢻ⛉Ꮫᩍ⫱◊✲ࢭࣥࢱ࣮ᰗᡞ㎰ሙ㸦ᒱ㜧 ┴ᒱ㜧ᕷᰗᡞ㸧㸪ᒱ㜧ᕷ␆⏘ࢭࣥࢱ࣮බᅬ㸦ᒱ㜧┴ᒱ㜧ᕷᳺὝ㸧㸪ᒱ㜧┴❧ᒱ㜧㎰ᯘ㧗 ➼Ꮫᰯ㸦ᒱ㜧┴ᮏᕢ㒆໭᪉⏫㸧࠾ࡼࡧ㛤⏣㧗ཎ᣺⯆බ♫ᮌ᭮㤿ࡢ㔛㸦㛗㔝┴㛤⏣㧗ཎ㸧 ࡢィ4 ࢝ᡤ࡛㣫⫱ࡉࢀ࡚࠸ࡓᮌ᭮㤿ࡢ㞤 17 㢌㸦No.1㹼17㸧ࢆᑐ㇟࡜ࡋࡓ㸦⾲ 1㸧ࠋ

17 ᐇ㦂1 ࡛ࡣ㸪⣅ࡢ᤼ἥᚋࡢ⤒㐣᫬㛫࡟క࠺ᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ௦ㅰ≀ྵ㔞ࡢኚ໬ ࢆㄪ࡭ࡿࡓࡵ࡟㸪ᮌ᭮㤿ࡢ㞤10 㢌㸦No.6㸪No.9㹼17㸧ࡢ┤⭠࠿ࡽ┤᥋⣅ࢆ᥇ྲྀᚋ㸪༑ ศ᧠ᢾࡋ㸪ࡑࡢ୰࠿ࡽ⣅ࢆ⣙30 g ᥇ྲྀᚋࡍࡄ࡟㸫20Υୗ࡛෾⤖ಖᏑࡋࡓࠋṧࡾࡢ⣅ヨ ᩱࡣ㸪20Υ࡟タᐃࡋࡓᜏ ჾෆ࡛ಖ ࡋࡓࠋ᥇ྲྀ┤ᚋ࡟෾⤖ಖᏑࡋࡓ᫬㛫ࢆ 0 ᫬㛫࡜ ࡋ㸪ࡑࡢᚋ㸪36 ᫬㛫ࡲ࡛ࡣ 2 ᫬㛫ẖ㸪36㹼72 ᫬㛫ࡲ࡛ࡣ 6 ᫬㛫ẖ㸪72㹼96 ᫬㛫ࡲ࡛ ࡣ12 ᫬㛫ẖ㸪96㹼166 ᫬㛫ࡲ࡛ࡣ 24 ᫬㛫ẖ࡟㸪ᜏ ჾෆࡢ⣅ࢆྠᵝ࡟⣙ 30 g ࡎࡘ᥇ ྲྀࡋ࡚㸫20Υୗ࡛෾⤖ಖᏑࡋࡓࠋ ᐇ㦂2 ࡛ࡣ㸪ᮌ᭮㤿 8 㢌㸦No.1㹼8㸧࠿ࡽ⣙ 1 ᖺ㛫⣅ࢆ㐌 2 ᅇ㸪እぢୖ᪂㩭࡞ࡶࡢࢆ ⣙100 g ᥇ྲྀࡋ㸪┤ࡕ࡟㸫20Υୗ࡛෾⤖ಖᏑࡋࡓࠋࡇࡢ࠺ࡕ 4 㢌㸦No.1㹼4㸧࠿ࡽࡣ㐌 1 ᅇ㸪⣅᥇ྲྀ᪥࡜ྠ᪥࡟㢁㟼⬦࠿ࡽ᥇⾑ࢆ⾜ࡗࡓࠋจᅛᚋࡢ⾑ᾮࢆ㸪4Υୗ 3,000 rpm ࡛10 ศ㛫㐲ᚰศ㞳ࡋ㸪⾑Ύࢆ㸫20Υୗ࡛෾⤖ಖᏑࡋࡓࠋᮏ◊✲ᮇ㛫୰࡟ᮌ᭮㤿ࡢ No.7 ࡜No.8 ࡛ࡣዷፎࡀㄆࡵࡽࢀ㸪ዷፎᮇࢆྵࡴ⣅ࢧࣥࣉࣝࡀᚓࡽࢀࡓࠋ 1㸫㸫2㸫2 ⾑୰ࡢࣉࣟࢪ࢙ࢫࢸࣟࣥྵ㔞ࡢ ᐃ ⾑୰P4⃰ᗘࡣ⛣ືᘧච␿Ⓨග ᐃ⿦⨨㸦PATHFAST㸪୕⳻໬Ꮫ࣓ࢹ࢖࢚ࣥࢫ㸧ࢆ⏝ ࠸࡚㸪໬ᏛⓎග㓝⣲ච␿ ᐃ㸦CLEIA㸧ἲ࡟ࡼࡾ ᐃࡋࡓࠋ ᐃ࡟ࡣᑓ⏝ࡢ P4࣮࢝ࢺ ࣜࢵࢪ㸦PF0181-K㸪୕⳻໬Ꮫ࣓ࢹ࢖࢚ࣥࢫ㸧ࢆ⏝࠸㸪ᐊ ୗ࡛⼥ゎࡋࡓ⾑Ύࢧࣥࣉࣝ ࢆ80 ȣl ౑⏝ࡋࡓࠋ 1㸫2㸫3 ⣅୰ࡢᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ௦ㅰ≀ࡢᢳฟ࡜ ᐃ ஝⇱ࡉࡏࡓ⣅ࢧࣥࣉࣝࢆ⣽࠿ࡃ⢊○ࡋ㸪ࡇࡢ࠺ࡕ0.100 g ࢆṇ☜࡟⛗ࡾྲྀࡾ㸪࢞ࣛ ࢫ〇ヨ㦂⟶࡟ධࢀ㸪ྛࢧࣥࣉࣝ࡟80㸣࣓ࢱࣀ࣮ࣝࢆ 5 ml ຍ࠼ࡓࠋࡇࢀࡽࢆ࣑࢟ࢧ࣮ 㸦Thermo-Mixer TM-127㸪ࢧ࣮ࣔࢽࢡࢫ㸧࡛ 30 ศ㛫᧠ᢾࡋ୍ᬌ㟼⨨ࡋࡓᚋ㸪4Υୗ 2,500 rpm ࡛ 10 ศ㛫㐲ᚰศ㞳ࢆ⾜࠸㸪࣓ࢱࣀ࣮ࣝᒙࢆศ㞳ࡋ࡚㸪ୖΎࡢ୍㒊ࢆ EIA buffer ࡛ 10 ಸᕼ㔘ࡋ࡚ ᐃ⏝ヨᩱ࡜ࡋࡓࠋࡇࢀࡽࡢ ᐃ⏝ヨᩱ୰ࡢᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ࠾ࡼ ࡧ௦ㅰ≀ࡢᐃ㔞ࡣ㓝⣲ච␿ ᐃ㸦EIA㸧ἲ࡟ࡼࡾ㸪P4㸪࢚ࢫࢺࣛࢪ࣮࢜ࣝ-17ș㸦E2㸧

18

࠾ࡼࡧ࢚ࢫࢺࣟࣥ㸦E1㸧⃰ᗘ࡜ࡋ࡚ ᐃࡋࡓࠋ

1㸫㸫2㸫4 㓝⣲ච␿ ᐃἲ

㸦1㸧ヨ⸆㢮ࡢㄪ〇

1㸧EIA buffer㸸 ࣜࣥ㓟Ỉ⣲஧ࢼࢺ࣒ࣜ࢘஧Ỉ࿴≀㸦MERCK㸧7.12 g ࡜ሷ໬ࢼࢺࣜ࢘

࣒㸦࿴ග⣧⸆ᕤᴗ㸧8.50 g ࢆ⣧Ỉ 1,000 ml ࡟⁐ゎࡋ㸦ㄪᩚ๓ pH 9.0 ⛬ᗘ㸧㸪pH 7.2 ࡟ㄪ

ᩚࡋࡓࠋࡑࡢᚋ㸪࢘ࢩ⾑Ύ࢔ࣝࣈ࣑࣭ࣥࣇࣛࢡࢩࣙࣥϬ㸦࿴ග⣧⸆ᕤᴗ㸧1 g ࢆຍ࠼㸪

౑⏝᫬ࡲ࡛ 4Υୗ࡛ಖᏑࡋࡓࠋ࢘ࢩ⾑Ύ࢔ࣝࣈ࣑ࣥ㸦BSA㸧ῧຍᚋࡣ 2 㐌㛫௨ෆ࡟౑

⏝ࡋࡓࠋ

2㸧Washing buffer㸸 ⣧Ỉ 2,000 ml ࡟ Tween80㸦Sigma-Aldrich㸧1g㸦⣙ 900ȣl㸧ࢆࢫ

ࢱ࣮࣮࡛ࣛ᧠ᢾࡋ࡞ࡀࡽᑡ㔞ࡎࡘ⁲ୗࡋࡓࠋࡉࡽ࡟30 ศ㛫᧠ᢾࡋࡓᚋ㸪౑⏝᫬ࡲ࡛ 4Υ

ୗ࡛ಖᏑࡋࡓࠋ

3㸧Coating buffer㸸 Ⅳ㓟ࢼࢺ࣒ࣜ࢘༑Ỉ࿴≀㸦࿴ග⣧⸆ᕤᴗ㸧2.574 g ࠾ࡼࡧⅣ㓟Ỉ

⣲ࢼࢺ࣒ࣜ࢘㸦࿴ග⣧⸆ᕤᴗ㸧1.758 g ࢆ㸪⣧Ỉ 600 ml ࡟⁐ゎࡋ㸦ㄪᩚ๓ pH 9.7 ⛬ᗘ㸧㸪

pH 9.6 ࡟ㄪᩚࡋࡓࠋ౑⏝᫬ࡲ࡛ 4Υୗ࡛ಖᏑࡋࡓࠋ

4㸧ࣖࢠᢠ࢘ࢧࢠ IgG ᢠయ㸦஧ḟᢠయ㸧㸸 ࣖࢠᢠ࢘ࢧࢠ IgG ᢠయ㸦2.4 mg/ml/vial㸪〇 ရ␒ྕ111-005-003㸪Jackson Immuno Research Laboratories㸧150ȣl ࡟ Coating buffer 204 ml ࢆຍ࠼㸪1360 ಸᕼ㔘ᢠయᾮࢆస〇ࡋࡓ㸦ࣉ࣮ࣞࢺ 20㸩Șᯛศࡢࢥ࣮ࢸ࢕ࣥࢢ⏝㸧ࠋ 5㸧ࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥᶆ‽ᾮ㸦ࢫࢱࣥࢲ࣮ࢻ㸧㸸 ྛࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥࡢ⣧ရ㸦࿴ ග⣧⸆ᕤᴗࡲࡓࡣSigma-Aldrich㸧10 mg ࢆ࢚ࢱࣀ࣮ࣝ㸦ヨ⸆≉⣭㸪࿴ග⣧⸆ᕤᴗ㸧10 ml ࡟⁐ゎࡋࡓ㸦1 mg/ml㸧ࠋࡇࡢ⁐ᾮ 1 ml ࡟⣧Ỉ 9 ml ࢆຍ࠼ 10 ಸᕼ㔘ࡋ㸦100ȣg/ml㸧㸪 ࡇࡢ࠺ࡕ1 ml ࡟ EIA buffer 9 ml ࢆຍ࠼࡚ࡉࡽ࡟ 10 ಸᕼ㔘ࡋࡓ㸦10ȣg/ml㸧ࠋࡑࡢᚋࡣ㸪 ྠᵝ࡟EIA buffer ࡛ 10 ಸࡎࡘᕼ㔘ࡋ㸪100 ng/ml ࡢಖᏑ⏝ᶆ‽ᾮࢆㄪ〇ࡋࡓࠋࡇࡢᶆ ‽ᾮࢆ300ȣl ࡎࡘศὀࡋ㸫30Υୗ࡛෾⤖ಖᏑࡋࡓࠋ౑⏝᫬࡟ࡣ㸪EIA buffer ࢆ⏝࠸࡚ 2 ಸᕼ㔘ࢆ⧞ࡾ㏉ࡋ㸪ྛ⃰ᗘࡢᶆ‽ᾮྛ 200ȣl ࢆస〇ࡋࡓ㸦⾲ 2㸧ࠋ

19 6㸧ࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥᢠయ㸦୍ḟᢠయ㸧㸸 ࢘ࢧࢠᢠࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ⾑Ύࡢ෾⤖ ஝⇱ရ㸦࠶ࡍ࠿〇⸆࣓ࢹ࢕࢝ࣝࡲࡓࡣࢥࢫࣔࣂ࢖࢜㸧ࢆ⣧Ỉ10 ml ࡛⁐ゎࡋࡓࠋࡉࡽ ࡟㸪EIA buffer ࢆ⏝࠸࡚ᕼ㔘ࡋࡓࡶࡢࢆ 800ȣl ࡎࡘศὀࡋ㸦ࣉ࣮ࣞࢺ 1 ᯛศ㸧㸪౑⏝᫬ ࡲ࡛㸫30Υୗ࡛෾⤖ಖᏑࡋࡓࠋ౑⏝᫬࡟ࡣ㸪EIA buffer 7.2 ml ࢆຍ࠼࡚ 10 ಸᕼ㔘ࡋ㸪 ⾲2 ࡟♧ࡋࡓ᭱⤊ᕼ㔘ಸ⋡࡜ࡋࡓࠋ࡞࠾㸪ྛᢠయ࡟࠾ࡅࡿྛ✀ࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ࡜ ࡢ஺ཫ⋡ࡣ㸪⾲3 ࡢ㏻ࡾ࡛࠶ࡿࠋ 7㸧ᶆ㆑ࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ㸦Horseradish peroxidase㸦HRP㸧ᶆ㆑ᢠཎ㸧㸸 HRP ᶆ㆑ ࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ1 ml/vial㸦ࢥࢫࣔࣂ࢖࢜㸧ࢆ HRP 㓝⣲άᛶ⥔ᣢಖᏑᾮ㸦GuardianTM

Peroxidase Conjugate Stabilizer / Diluent㸪Pierce㸧ࢆ⏝࠸࡚ᕼ㔘ࡋ㸪4Υୗ࡛ಖᏑࡋࡓࠋ

౑⏝᫬࡟ࡣ㸪ࡇࡢ࠺ࡕ80ȣl ࢆ EIA buffer 8.0 ml ࡛⣙ 100 ಸᕼ㔘ࡋ㸦ࣉ࣮ࣞࢺ 1 ᯛศ㸧㸪 ⾲2 ࡟♧ࡋࡓ᭱⤊ᕼ㔘ಸ⋡࡜ࡋࡓࠋ 8㸧ⓎⰍᾮ 㸺ᇶ㉁ᾮA㸼 㐣㓟໬ᒀ⣲㸦Sigma-Aldrich㸧0.5 g㸪ࣜࣥ㓟Ỉ⣲஧ࢼࢺ࣒ࣜ࢘஧Ỉ࿴≀ 㸦Merck㸧9.0 g ࠾ࡼࡧࢡ࢚ࣥ㓟୍Ỉ࿴≀㸦Sigma-Aldrich㸧5.15 g ࢆ㸪⣧Ỉ 500 ml ࡟⁐ ゎࡋࡓࠋ౑⏝᫬ࡲ࡛4Υୗ࡛㐽ගಖᏑࡋࡓࠋ 㸺ᇶ㉁ᾮB㸼 3,3',5,5'-ࢸࢺ࣓ࣛࢳࣝ࣋ࣥࢳࢪࣥ㸦Sigma-Aldrich㸧0.25 g㸦0.002M㸧㸪ࢪ ࣓ ࢳ ࣝ ࢫ ࣝ ࣍ ࢟ ࢩ ࢻ 㸦Sigma-Aldrich 㸧 20 ml 㸦 4 㸣 㸧 ࠾ ࡼ ࡧ ࢡ ࢚ ࣥ 㓟 ୍ Ỉ ࿴ ≀ 㸦Sigma-Aldrich㸧5.15 g㸦0.05M㸧ࢆ㸪⣧Ỉ 480 ml ࡟⁐ゎࡋࡓࠋ౑⏝᫬ࡲ࡛ 4Υୗ࡛㐽 ගಖᏑࡋࡓࠋ 㸺ⓎⰍᾮ㸼 ᇶ㉁ᾮA ࠾ࡼࡧ B ࢆྛ 5 ml㸦ࣉ࣮ࣞࢺ 1 ᯛศ㸧ࡎࡘ౑⏝┤๓࡟㐽ග≧ែ ࡛ΰྜࡋ᧠ᢾࡋࡓࠋ 9㸧ⓎⰍ೵Ṇᾮ㸸 ⃰◲㓟㸦࿴ග⣧⸆ᕤᴗ㸧20 ml ࢆịỈ෭ୗ࡛ 700 ml ࡢ⣧Ỉ࡟ᑡ㔞ࡎ ࡘຍ࠼࡚1N ࡜ࡋ㸪౑⏝᫬ࡲ࡛ 4Υୗ࡛ಖᏑࡋࡓࠋ 㸦 㸦2㸧ࣉ࣮ࣞࢺࢥ࣮ࢸ࢕ࣥࢢ

20

Coating buffer ࡛ᕼ㔘ࡋࡓ஧ḟᢠయࢆࢫࢱ࣮࣮࡛ࣛ᧠ᢾࡋ࡞ࡀࡽ㸪96 ✰࣐࢖ࢡࣟࣉ࣮ࣞ ࢺ㸦Nunc ࢖࣒ࣀࣉ࣮ࣞࢺ㸪〇ရ␒ྕ 442404㸪Nunc㸧ࡢྛ࢙࢘ࣝ࡟ 100ȣl ࡎࡘศὀࡋ

ࡓࠋ20Υ࡟タᐃࡋࡓప ᜏ ჾෆ࡛ 2 ᫬㛫࢖࣮ࣥ࢟ࣗ࣋ࢺࡋ㸪஧ḟᢠయࢆ࢙࢘ࣝ࡟ᅛ

ᒙ໬ࡋࡓࠋ࢙࢘ࣝෆࡢCoating buffer ࢆ᏶඲࡟㝖ཤࡋ㸪ࣉ࣮ࣞࢺಖᏑᾮ࡜ࡋ࡚ EIA buffer

ࢆྛ࢙࢘ࣝ200ȣl ࡎࡘຍ࠼㸪ࣉ࣮ࣞࢺࣇ࢕࣒࡛ࣝࣉ࣮ࣞࢺࢆࢩ࣮ࣝࡋ㸪20Υ࡟タᐃࡋ ࡓᜏ ჾෆ࡛30 ศ㛫࢖࣮ࣥ࢟ࣗ࣋ࢺࢆ⾜ࡗࡓࠋ౑⏝᫬ࡲ࡛ 4Υୗ࡛ಖᏑࡋࡓࠋ 1㸫㸫2㸫5 㓝⣲ච␿ ᐃἲࡢᡭ㡰࡜࣍ࣝࣔࣥ⃰ᗘࡢィ⟬ ஧ḟᢠయࢆᅛᒙ໬ࡋࡓ࣐࢖ࢡࣟࣉ࣮ࣞࢺෆࡢࣉ࣮ࣞࢺಖᏑᾮ㸦EIA buffer㸧ࢆ࢔ࢵࢭ࢖ ┤๓࡟㝖ཤࡋ㸪Washing buffer ࢆྛ࢙࢘ࣝ࡟ 300ȣl ࡎࡘຍ࠼࢙࢘ࣝෆࢆὙίࡋࡓ㸦ࣉ ࣮ࣞࢺࣜࣥࢫ㸧ࠋࣉ࣮ࣞࢺࣜࣥࢫࢆ2 ᅇ⾜ࡗࡓᚋ㸪Washing buffer ࢆ᏶඲࡟ྲྀࡾ㝖࠸ࡓࠋ ྛ࢙࢘ࣝ࡟ヨᩱࡲࡓࡣᶆ‽ᾮࢆ20ȣl ධࢀ㸪୍ḟᢠయ㸪HRP ᶆ㆑ᢠཎࡢ㡰࡟ྛ࢙࢘ࣝ ࡟100ȣl ศὀࡋࡓ㸦⾲ 4㸧ࠋࡑࡢᚋ㸪ࣉ࣮ࣞࢺࣇ࢕࣒࡛ࣝࣉ࣮ࣞࢺࢆࢩ࣮ࣝࡋ㸪4Υୗ ࡛ 24 ᫬㛫࢖࣮ࣥ࢟ࣗ࣋ࢺࢆ⾜ࡗࡓࠋ࢖࣮ࣥ࢟ࣗ࣋ࢺᚋ㸪࢙࢘ࣝෆࡢ⁐ᾮࢆ㝖ཤࡋ㸪

Washing buffer 300ȣl ࢆ⏝࠸࡚㸪ࣉ࣮ࣞࢺࣜࣥࢫࢆィ 4 ᅇ⾜ࡗࡓࠋWashing buffer ࢆ᏶

඲࡟ྲྀࡾ㝖࠸ࡓᚋ㸪ⓎⰍᾮࢆྛ࢙࢘ࣝ࡟ 100ȣl ศὀࡋࡓ㸦⾲ 4㸧ࠋࣉ࣮ࣞࢺࣇ࢕࣒ࣝ ࡛ࣉ࣮ࣞࢺࢆࢩ࣮ࣝࡋ㸪37Υ࡟タᐃࡋࡓᜏ ჾෆ࡛ 60 ศ㛫࢖࣮ࣥ࢟ࣗ࣋ࢺࢆ⾜ࡗࡓࠋ ࢖࣮ࣥ࢟ࣗ࣋ࢺᚋࡍࡄ࡟ⓎⰍ೵Ṇᾮࢆྛ࢙࢘ࣝ࡟100ȣl ຍ࠼㸪ⓎⰍࢆ೵Ṇࡉࡏࡓ㸦⾲ 4㸧ࠋ྾ග࣐࢖ࢡࣟࣉ࣮ࣞࢺ࣮ࣜࢲ࣮㸦iMark㸪ࣂ࢖࣭࢜ࣛࢵࢻࣛ࣎ࣛࢺ࣮ࣜࢬ㸧࡟ࡼࡾ㸪 Ἴ㛗 450 nm ࡟࠾ࡅࡿ྾ගᗘࢆ ᐃࡋ㸪ᑓ⏝ ᐃゎᯒࢯࣇࢺ࢙࢘࢔㸦࣐࢖ࢡࣟࣉ࣮ࣞ ࢺ࣐ࢿ࣮ࢪ࣮ࣕ6.0㸪ࣂ࢖࣭࢜ࣛࢵࢻࣛ࣎ࣛࢺ࣮ࣜࢬ㸧ࢆ⏝࠸࡚ᶆ‽᭤⥺ࢆసᡂࡋ㸪ྛ ヨᩱࡢ࣍ࣝࣔࣥ⃰ᗘࢆィ⟬ࡋࡓࠋྛࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥࡢᶆ‽᭤⥺ࡢ୍౛ࢆ㸪ᅗ1 ࡟ ♧ࡋࡓࠋ 1㸫3 ࢹ࣮ࢱศᯒ

21

⣅୰ࡢࡍ࡭࡚ࡢ್࣍ࣝࣔࣥࡣ㸪஝⇱⣅1 g ᙜࡓࡾࡢྵ㔞࡜ࡋ࡚ồࡵࡓࠋ㠀ዷፎᮌ᭮

㤿ࡢ┤⭠⣅ࡢ㯤యᮇ࡜㠀㯤యᮇࡣ㸪Moreira et al.,㸦2001㸧࡜ Rafacz et al.,㸦2013㸧ࡢィ

⟬᪉ἲࢆ⏝࠸࡚Ỵᐃࡋࡓࠋࡍ࡞ࢃࡕ㸪ࡲࡎ඲ಶయࡢⓎ᝟࿘ᮇࡢ⣅୰P4ྵ㔞ࡢᖹᆒ㸩1.5 SD㸦ᶆ‽೫ᕪ㸧ࢆィ⟬ࡋ㸪ࡑࡢྵ㔞ࡼࡾ኱ࡁ࠸ࡍ࡭࡚ࡢ್ࢆ᤼㝖ࡋࡓࠋᖹᆒ㸩1.5 SD ್ࢆ㉸࠼ࡿ್ࡀ࡞ࡃ࡞ࡿࡲ࡛ࡑࢀࢆ⧞ࡾ㏉ࡋࡓࠋ᭱ᚋ࡟ṧࡗࡓᖹᆒ㸩1.5 SD㸦0.95 ȣg/g㸧ࢆᇶᗏ್࡜ࡋ㸪ࡑࢀࢆ㉸࠼ࡓ᫬ᮇࢆ㯤యᮇ㸪ࡑࢀ௨እࢆ㠀㯤యᮇ࡜ࡋ࡚ᢅࡗࡓࠋ ┤⭠⣅ࡢ᥇ྲྀ┤ᚋ㸦0 ᫬㛫㸧࡜ࡑࢀ௨㝆ࡢ᫬㛫ᖏࡢ⣅୰ᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ௦ㅰ ≀ྵ㔞ࡢᖹᆒ್㛫ࡢ᭷ពᕪ᳨ᐃ࡟ࡣ㸪Tukey Kramer ἲ࡟ࡼࡾከ㔜ẚ㍑ࢆ⾜࠸㸪༴㝤⋡ 1%㸦p<0.01㸧࡜ 5%㸦p<0.05㸧᳨࡛ᐃ⤖ᯝࢆ♧ࡋࡓࠋ⾑୰࡜⣅୰ࡢ P4್ࡢ┦㛵㛵ಀࡣ㸪 ࣆ࢔ࢯࣥࡢ✚⋡┦㛵ಀᩘࢆ⟬ฟࡋ㸪┦㛵ࡢ᭷ពᕪ᳨ᐃࢆ⾜ࡗࡓࠋ ୍⯡ⓗ࡟ᐙ␆࣐࢘ࡢ༸⬊ᮇ㸦㠀㯤యᮇ㸧ࡢ⾑୰P4⃰ᗘࡣ1 ng/ml ௨ୗ࡛࠶ࡿࡓࡵ㸪 P4⃰ᗘୖ᪼┤๓ࡢ 1 ng/ml ௨ୗࡢ್㸦ᮏ◊✲࡛ࡣྛ࿘ᮇࡢ᭱ప್࡜࡞ࡗࡓ㸧ࢆ♧ࡋࡓ

ࢧࣥࣉࣝࡢ᥇ྲྀ᪥㸦᥇⾑᪥㸧ࢆ᤼༸᪥࡜௬ᐃࡋࡓ㸦Meinecke et al., 1987; Amer et al.,

2008䠅ࠋ༸ᕢ࿘ᮇ㸦᤼༸࿘ᮇ㸧ࡣண ᤼༸᪥࠿ࡽḟࡢ࿘ᮇࡢண ᤼༸᪥ࡲ࡛ࡢ᪥ᩘ࡜ࡋ

࡚⟬ฟࡋࡓࠋ⣅୰ P4ྵ㔞࡟ࡼࡿ༸ᕢ࿘ᮇࡢ᪥ᩘࡢィ⟬ࡣ㸪⾑୰࡜⣅୰ࡢ P4ືែ࡜ẚ

㍑ࡋࡓᮌ᭮㤿4 㢌㸦No.1㹼4㸧ࡢ⤖ᯝࢆᇶ࡟㸪ྛ࿘ᮇࡢ᭱ప್ࢆ♧ࡋࡓ᪥ࢆᇶ‽࡜ࡋࡓࠋ

 

22 ⾲1 ⾑୰ࡲࡓࡣ⣅୰ࡢ࣍ࣝࣔࣥ ᐃࢆ⾜ࡗࡓື≀✀ࡢಶయ᝟ሗ࡜ࢧࣥࣉࣜࣥࢢ≧ἣ ✀ ಶయ␒ྕ ⾑⤫Ⓩ㘓␒ྕ ಶయྡ 㣫⫱᪋タ ᛶู ⏕ᖺ᭶᪥ ⦾ṪṔ ࢧࣥࣉࣝ᥇ྲྀ ᅜෆ ᅜ㝿 ⾑ᾮ ⣅ ᮌ᭮㤿 No.1 21391ᇞ0011 ࠶ࡍ࠿ ᰗᡞ㎰ሙ ܲ 2001/3/20 㸫㸫 ۑ ۑ No.2 20291ᇞ0012 ᖾⰼ ㎰ᯘ㧗ᰯ ܲ 1990/5/1 㸫㸫 ۑ ۑ No.3 21391ᇞ0004 ⸨ⰼ ㎰ᯘ㧗ᰯ ܲ 2001/4/19 㸫㸫 ۑ ۑ No.4 㸫 ᳺጲ ␆⏘ࢭࣥࢱ࣮ ܲ 1989/4/6 ۑ ۑ ۑ No.5 21291ᇞ0003 ᑠኟ 㛤⏣㧗ཎ ܲ 2000/4/19 㸫 ۑ No.6 21391ᇞ0005 ✵ 㛤⏣㧗ཎ ܲ 2001/3/28 㸫 ۑ No.7 21391ᇞ0006 ᫓ⴌ 㛤⏣㧗ཎ ܲ 2001/3/28 ۑ ۑ No.8 21091ᇞ0004 ⳯ࠎ 㛤⏣㧗ཎ ܲ 1998/4/24 ۑ ۑ No.9 21391ᇞ0007 ᮍ᮶ 㛤⏣㧗ཎ ܲ 2001/4/24 㸫 ۑ No.10 21191ᇞ0003 ࠶ࡸࡵ 㛤⏣㧗ཎ ܲ 1999/6/6 ۑ ۑ No.11 2 ඖ 91ᇞ0011 ᐆ⸨ 㛤⏣㧗ཎ ܲ 1989/7/25 ۑ ۑ No.12 21091ᇞ0005 ༓⛅ 㛤⏣㧗ཎ ܲ 1998/4/5 ۑ ۑ No.13 㸫 㕥ⰼ 㛤⏣㧗ཎ ܲ 2007/4/18 㸫 ۑ No.14 20291ᇞ0004 ⚟Ἠ 㛤⏣㧗ཎ ܲ 1990/5/27 ۑ ۑ No.15 20591ᇞ0014 ᐩⰼ 㛤⏣㧗ཎ ܲ 1993/4/11 ۑ ۑ No.16 20291ᇞ0009 ጲⱝ 㛤⏣㧗ཎ ܲ 1990/5/5 ۑ ۑ No.17 20691ᇞ0005 ᫂ⰼ 㛤⏣㧗ཎ ܲ 1994/5/3 ۑ ۑ ᰗᡞ㎰ሙ㸸ᒱ㜧኱Ꮫᛂ⏝⏕≀⛉Ꮫ㒊㝃ᒓᒱ㜧ࣇ࢕࣮ࣝࢻ⛉Ꮫᩍ⫱◊✲ࢭࣥࢱ࣮ᰗᡞ㎰ሙ㸪㎰ᯘ㧗ᰯ㸸 ᒱ㜧┴❧ᒱ㜧㎰ᯘ㧗➼Ꮫᰯ㸪␆⏘ࢭࣥࢱ࣮㸸ᒱ㜧ᕷ␆⏘ࢭࣥࢱ࣮බᅬ㸪㛤⏣㧗ཎ㸸㛤⏣㧗ཎ᣺⯆බ♫ᮌ ᭮㤿ࡢ㔛㸪㸫㸸୙᫂㸪ۑ㸸࠶ࡿ

23 ⾲ 2 㓝⣲ච␿ ᐃ࡟࠾࠸࡚౑⏝ࡋࡓྛࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥࡢᶆ‽≀㉁㸪ᢠయ࠾ࡼࡧ HRP ᶆ㆑ࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ࡜ࡑࢀࡽࡢ౑⏝᫬⃰ᗘ ࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ⣧ရ ᶆ‽᭤⥺ࡢ⃰ᗘ⠊ᅖ ࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥᢠయ㸦୍ḟᢠయ㸧 HRP ᶆ㆑ࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ ౑⏝᫬ࡢ ᭱⤊ᕼ㔘ಸ⋡ ࣉࣟࢪ࢙ࢫࢸࣟࣥ pregn-4-ene-3,20-dione 㸦161-14531㸪࿴ග⣧⸆ᕤᴗ㸧 0.049㸦St.1㸧㹼50 ng/ml㸦St.11㸧 anti progesterone-3-CMO-BSA 㸦LC-28㸪࠶ࡍ࠿〇⸆࣓ࢹ࢕࢝ࣝ㸧 100 ୓ಸ progesterone-3-CMO-HRP 㸦FKA301㸪ࢥࢫࣔࣂ࢖࢜㸧 100 ୓ಸ ࢚ࢫࢺࣛࢪ࣮࢜ࣝ-17β estra-1,3,5(10)-triene-3,17β-diol 㸦052-04041㸪࿴ග⣧⸆ᕤᴗ㸧 0.049㸦St.1㸧㹼25 ng/ml㸦St.10㸧 anti estradiol-3-CME-BSA 㸦FKA236-E㸪ࢥࢫࣔࣂ࢖࢜㸧 300 ୓ಸ estradiol-3-CPE-HRP 㸦FKA235㸪ࢥࢫࣔࣂ࢖࢜㸧 400 ୓ಸ ࢚ࢫࢺࣟࣥ 3-hydroxyestra-1,3,5(10)-triene-17-one 㸦E9750㸪Sigma-Aldrich㸧 0.024㸦St.1㸧㹼12.5 ng/ml㸦St.10㸧 anti estrone-3-CME-BSA 㸦FKA234-E㸪ࢥࢫࣔࣂ࢖࢜㸧 400 ୓ಸ estrone-3-CME-HRP 㸦FKA233㸪ࢥࢫࣔࣂ࢖࢜㸧 200 ୓ಸ 䝔䝇䝖䝇䝔䝻䞁 17β-hydroxyandrost-4-en-3-one 䠄208-08341䠈࿴ග⣧⸆ᕤᴗ䠅 0.098㸦St.1㸧㹼50 ng/ml㸦St.10㸧 anti testosterone-11α-succinate-BSA 䠄ED131䠈䛒䛩䛛〇⸆䝯䝕䜱䜹䝹䠅 200 ୓ಸ testosterone-11α-succinate-HRP 䠄FKA103䠈䝁䝇䝰䝞䜲䜸䠅 100 ୓ಸ

24 ⾲3 ࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥࡢྛᢠయ࡟࠾ࡅࡿྛ✀ࢫࢸࣟ࢖ࢻ࡜ࡢ஺ཫ⋡ (a) ࣉࣟࢪ࢙ࢫࢸࣟࣥᢠయ 㸦LC-28㸪࠶ࡍ࠿〇⸆࣓ࢹ࢕࢝ࣝ㸧 ࢫࢸࣟ࢖ࢻ ஺ཫ⋡㸦㸣㸧 Progesterone 100  5α-pregnanedione 62.2 Pregnenolone 6.26 11-deoxycorticosterone 3.88 17α-hydroxyprogesterone 2.25 11α-hydroxyprogesterone 1.23 20α-hydroxyprogesterone 0.51 Testosterone 0.18 11-deoxycortisol 0.14 Androstenedione 0.12 5α-dihydrotestosterone 0.12 Corticosterone 0.07 17α-hydroxypregnenolone 0.05 Dehydroepiandrostendione 0.04 Cortisol 0.01 Cortisone 0.01 Aldosterone 0.01 Estrone 0.01 estradiol-17β 0.01 Estriol 0.01 Androsterone 0.01 (c) ࢚ࢫࢺࣟࣥᢠయ 㸦FKA234-E㸪ࢥࢫࣔࣂ࢖࢜㸧 ࢫࢸࣟ࢖ࢻ ஺ཫ⋡㸦㸣㸧 Estrone 100 estrone-3-sulfate 40 estrone-3-glucuronide 15 Estradiol 1.2 estradiol-3-glucuronide 0.5 estradiol-3-sulfate 0.5 estradiol-17-glucuronide 0.05 estradiol-17-sulfate 0 Estriol 0 estriol-16-glucuronide 0 estriol-sulfate 0 Testosterone 0 (b) ࢚ࢫࢺࣛࢪ࣮࢜ࣝ-17β ᢠయ 㸦FKA236-E㸪ࢥࢫࣔࣂ࢖࢜㸧 ࢫࢸࣟ࢖ࢻ ஺ཫ⋡㸦㸣㸧 estradiol-17β 10 0 estradiol-3-glucuronide 56. 3 estradiol-3-sulfate 26.8 estrone-3-glucuronide 1.20 estrone-3-sulfate 0.86 estrone 0.80 estriol 0.50 testosterone 0.05 pregnenolone 0 progesterone 0 cortisol 0 cortisone 0 testosterone 0 4-androstenedione 0 dehydroepiandrosterone 0 dihydrotestosterone 0

25 ⾲4 㓝⣲ච␿ ᐃἲ࡟⏝࠸ࡓヨᩱ࠾ࡼࡧྛヨ⸆ࡢ౑⏝㔞 EIA Buffer ᶆ‽ᾮ ࡲࡓࡣヨ ᩱ ୍ḟᢠయ HRP ᶆ㆑ᢠཎ ⓎⰍᾮ ⓎⰍ ೵Ṇᾮ 㸦μl㸧 㸦μl㸧 㸦μl㸧 㸦μl㸧 㸦μl㸧 㸦μl㸧 NSB㸦non-specific binding㸧 ್ 120 㸫 㸫 100 100 100 Bo 㸦maximum binding㸧್ 20 㸫 100 100 100 100 ᶆ‽ᾮ 㸫 20 100 100 100 100  ᐃヨᩱ 㸫 20 100 100 100 100

26

27 1㸫㸫4 ⤖ᯝ 1㸫4㸫1 ⣅୰ࡢᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ௦ㅰ≀ྵ㔞ࡢ⤒᫬ⓗኚ໬㸦ᐇ㦂 1㸧 ᮌ᭮㤿ࡢ㯤యᮇ㸦n㸻6㸧ࡢ⣅୰ P4ྵ㔞ࡣ᥇ྲྀᚋ6 ᫬㛫ࡲ࡛࡯ࡰ୍ᐃࡢ್࡛࠶ࡗࡓࡀ㸪 ࡑࢀ௨㝆࡟ቑຍࡀࡳࡽࢀ48 ᫬㛫௨㝆ࡣῶᑡࡋࡓ㸦ᅗ 1㸪A1㸧ࠋ᥇ྲྀ᫬㸦0 ᫬㛫㸧ࡢ⣅ ୰ P4௦ㅰ≀ྵ㔞࡟ẚ࡭㸪24㹼48 ᫬㛫ࡢࢧࣥࣉ࡛ࣝࡣ㸪᭷ព࡟㧗࠸್ࢆ♧ࡋࡓࠋ㠀㯤 యᮇ㸦n㸻4㸧ࡢ⣅୰ P4ྵ㔞ࡢኚ໬ࡶ㯤యᮇ࡜࡯ࡰྠᵝࡢഴྥ࡛࠶ࡗࡓࡀ㸪᭷ពᕪࡣㄆ ࡵࡽࢀ࡞࠿ࡗࡓࠋ㯤యᮇࡢ⣅୰E2࡜E1ྵ㔞ࡣࡑࢀࡒࢀ᤼ἥᚋ60 ᫬㛫ࡲ࡛࡯ࡰ୍ᐃ࡛ ࠶ࡗࡓࡀ㸪ࡑࢀ௨㝆ቑຍഴྥࢆ♧ࡋ㸪E2ࡣ96㹼168 ᫬㛫㸪E1ࡣ84㹼168 ᫬㛫ࡢࢧࣥࣉ ࡛ࣝ᭷ព࡟㧗࠸್ࢆ♧ࡋࡓ㸦ᅗ1㸪A2 ࡜ A3㸧ࠋ㠀㯤యᮇࡢ⣅୰ E2࡜E1ྵ㔞ࡶ࡯ࡰྠ ᵝࡢኚ໬ࢆ♧ࡋࡓࠋ 1㸫4㸫2 ᮌ᭮㤿ࡢ⣅୰ࣉࣟࢪ࢙ࢫࢸࣟࣥྵ㔞ࡢືែ㸦ᐇ㦂 2㸧 㞤ᮌ᭮㤿No.1㹼4 ࡢ୍Ⓨ᝟࿘ᮇࡢ⾑୰࡜⣅୰ࡢ P4ືែࢆᅗ 2 ࡟♧ࡋ㸪୧ P4್ࡢ㛫 ࡟ࡣ㸪┦㛵ಀᩘ 0.46 ࡢ᭷ព࡞ṇࡢ┦㛵ࡀㄆࡵࡽࢀࡓ㸦p<0.01㸧ࠋࡲࡓ㸪㞤 6 㢌㸦No.1 㹼6㸧࡟࠾ࡅࡿ⣅୰࡜⾑୰ P4್ࡢᖺ㛫ືែࢆᅗ3 ࡟♧ࡋࡓࠋಶయ No.1㸪No.2㸪No.5 ࠾ ࡼࡧNo.6 ࡢ⾑୰ࡲࡓࡣ⣅୰ P4್ࡣ5 ᭶ࡲࡓࡣ 6 ᭶࠿ࡽ 11 ᭶ࡲ࡛ࡢᮇ㛫㸪࿘ᮇⓗ࡞ኚ ືࢆ♧ࡋࡓࡀ㸪No.3 ࡜ No.4 ࡛ࡣ࡯ࡰᖺ㛫ࢆ㏻ࡋ࡚࿘ᮇⓗ࡞ኚືࡀㄆࡵࡽࢀࡓ㸦ᅗ 3㸧ࠋ ࡞࠾㸪No.3 ࡣ஺ᑿࡋ࡚࠸࡞࠸㸦㞝࡜ࡣྠᒃࡉࡏ࡚࠸࡞࠸㸧࡟ࡶ㛵ࢃࡽࡎ㸪⾑୰ P4⃰ ᗘࡣ7㹼10 ᭶࡟ᣢ⥆ⓗ࡟㧗࠸್ࢆ♧ࡋࡓࠋྠ᫬ᮇࡢ⣅୰ P4ྵ㔞ࡶ┦ᑐⓗ࡟㧗࠸್ࢆ♧ ࡋࡓ㸦ᅗ 3㸧ࠋ ᐃᮇ㛫୰ࡢ⾑୰࡜⣅୰ࡢ P4್ࡢືែ࠿ࡽ⟬ฟࡋࡓ༸ᕢ࿘ᮇࡢᖹᆒ್ 㸦sᶆ‽೫ᕪ㸪SD㸧ࡣ㸪ࡑࢀࡒࢀ 24.3s1.3 ᪥㛫㸦⠊ᅖ㸸21㹼31 ᪥㛫㸪n㸻4 㢌㸧࡜ 23.6s1.7 ᪥㛫㸦14㹼35 ᪥㛫㸪n㸻6㸧࡛࠶ࡗࡓࠋ

ᮌ᭮㤿No.7 ࡜ No.8 ࡢዷፎᮇ࡟࠾ࡅࡿ⣅୰ P4㸪E2࠾ࡼࡧE1ࡢືែࢆᅗ 4 ࡜ᅗ 5 ࡟

♧ࡋࡓࠋ⣅୰P4ྵ㔞ࡣ㸪ᮌ᭮㤿࡛ࡣ᭱⤊஺ᑿ᪥࠿ࡽ50㹼60 ᪥┠ࡲ୍࡛ᐃࡢ್ࢆ᥎⛣

28 ቑຍࡋฟ⏘┤๓࡟㡬್ࢆ♧ࡋࡓࠋ⣅୰E2࡜E1ྵ㔞ࡣ㸪᭱⤊஺ᑿᚋ⣙90 ᪥┠࠿ࡽ㢧ⴭ ࡟ୖ᪼ࢆ㛤ጞࡋ㸪ࡑࢀࡒࢀ 160 ᪥┠㹼220 ᪥┠ࡢ㛫࡟㡬್ࢆ♧ࡋ㸪ࡑࡢᚋᚎࠎ࡟ῶᑡ ࡋࡓࠋ࡞࠾㸪᭱⤊஺ᑿ᪥࠿ࡽ⟬ฟࡋࡓᮌ᭮㤿2 㢌㸦No.7㸪No.8㸧ࡢዷፎᮇ㛫ࡣ 315 ᪥ 㛫࡜348 ᪥㛫࡛࠶ࡗࡓࠋ 1㸫㸫5 ⪃ᐹ ᮏ◊✲࡛ࡣ㸪ࡲࡎ⣅୰ࡢᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ௦ㅰ≀ྵ㔞ࡢ⤒᫬ⓗ࡞ኚ໬࡟ࡘ࠸࡚ ᫂ࡽ࠿࡟ࡋࡓࠋ㯤యᮇ࡜㠀㯤యᮇࡢ⣅୰P4ྵ㔞ࡣ8 ᫬㛫௨㝆ᚎࠎ࡟ቑຍࡋ㸪㯤యᮇࡢ ⣅୰㸦㠀㯤యᮇࡶ࡯ࡰྠᵝ㸧E2࡜ E1ࡣ84 ᫬㛫௨㝆᭷ពⓗ࡟ቑຍࡋࡓࠋࢩࣂࣖࢠ㸪࢘ ࢩ㸪࣐࢘࠾ࡼࡧࣈࢱ࡛ࡣ㸪⣅୰ࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ௦ㅰ≀ྵ㔞ࡀ⤒᫬ⓗ࡟ኚ໬ࡍࡿࡇ ࡜ࡀሗ࿌ࡉࢀ࡚࠾ࡾ㸪ࡇࢀࡣ⣅୰ࡢ⣽⳦࡜㓝⣲ࡢᙳ㡪࡟ࡼࡾ⏕ࡌࡿࡶࡢ࡜⪃࠼ࡽࢀ࡚ ࠸ࡿ㸦Wasser et al., 1988; Hirata and Mori, 1995; Möstl et al., 1999; Kharn et al., 2002㸧ࠋࡇ ࢀࡽࡢᙳ㡪ࢆ㑊ࡅࡿࡓࡵ࡟㸪⣅ࢆ෭෾ಖᏑࡋ࡚⣽⳦ࡢάືࢆ೵Ṇࡉࡏ㸪ࡑࡢ㓝⣲ࢆ୙ ά໬ࡉࡏࡿࡇ࡜ࡸ⣅୰࡟࢚ࢱࣀ࣮ࣝࢆῧຍࡍࡿࡇ࡜࡛ࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ௦ㅰ≀ྵ㔞 ࡢ⤒᫬ኚ໬ࢆᢚ࠼ࡿࡇ࡜ࡀ࡛ࡁࡿ࡜ሗ࿌ࡉࢀ࡚࠸ࡿ㸦Wasser et al.,1988; Kharn et al., 2002㸧ࠋᮏ◊✲࡟࠾࠸࡚⣅୰࣍ࣝࣔࣥ௦ㅰ≀ࡣ㸪᥇ྲྀᚋࡢ᫬㛫⤒㐣࡜࡜ࡶ࡟ኚ໬ࡋࡓࡀ㸪 ࡇࢀࡣ⣽⳦࡜㓝⣲ࡢస⏝࡟ࡼࡾ㸪౑⏝ࡋࡓᢠయ࡜஺ཫ཯ᛂࡍࡿ௦ㅰ≀ࡢ㔞ࡀቑ࠼ࡓࡶ ࡢ࡜⪃࠼ࡓࠋࡍ࡞ࢃࡕ㸪ᮌ᭮㤿࡛ࡣ⣅୰࣍ࣝࣔࣥྵ㔞ࢆṇ☜࡟ ᐃࡍࡿࡓࡵ࡟ࡣ㸪࡛ ࡁࡿ㝈ࡾ᪩ᮇ࡟㸪ᑡ࡞ࡃ࡜ࡶ6 ᫬㛫௨ෆ࡟෭෾ಖᏑࡍࡿᚲせࡀ࠶ࡿࠋ ㏻ᖖ㸪ᐙ␆ࡢ࣐࢘ࡣᏘ⠇⦾Ṫື≀࡛࠶ࡾ㸪໭༙⌫࡛ࡣ୍⯡ⓗ࡟5㹼10 ᭶ࡲ࡛ࡀ⦾Ṫ Ꮨ⠇࡛࠶ࡿ㸦England, 2005㸧ࠋᮏ◊✲࡛ࡣᮌ᭮㤿 4 㢌㸦No.1㸪No.2㸪No.5㸪No.6㸧ࡢ P4್ࡢኚື࡟ᇶ࡙ࡃ⦾ṪᏘ⠇ࡣࡇࢀࡽࡢሗ࿌࡜࡯ࡰ୍⮴ࡋࡓࠋࡋ࠿ࡋ㸪No.3 ࡜ No.4 ࡢ⾑୰࡜⣅୰P4್ࡣ㸪ᖺ㛫ࢆ㏻ࡋ࡚࿘ᮇⓗ࡞ኚືࢆ⧞ࡾ㏉ࡋࡓࠋள⇕ᖏᆅᇦ࡟㣫⫱ࡉ ࢀ࡚࠸ࡿᐙ␆࣐࢘ࡢ㸦ࢫࢺࢵࢡရ✀㸧ࡢ୍㒊࡟ࡣ⦾ṪᏘ⠇ࡣ᫂░࡟ㄆࡵࡽࢀࡎ㸦Dowsett et al., 1993㸧㸪ࡲࡓ஑ᕞᆅ᪉ࡢ㔜✀㤿㸦࣋ࣝࢩࣗࣟࣥ⣔㸪ࣈࣝࢺࣥ⣔㸧࡟࠾࠸࡚ࡶ㸪෤

29 ࡢ㠀⦾Ṫᮇ࡟୍㒊ࡢಶయ࡛ࡣ᤼༸ࡸ㯤యᙧᡂࡀ☜ㄆࡉࢀ࡚࠸ࡿ㸦୰ᮧ䜙, 2002䠅ࠋࡇࢀ ࡽࡢࡇ࡜࡟ࡼࡗ࡚㸪ᮌ᭮㤿ࡣ୍⯡ⓗ࡟෤ࡀ㠀⦾Ṫᮇ࡛࠶ࡿࡀ㸪࡞࠿࡟ࡣ෤Ꮨ࡛ࡶ᤼༸ ࡸ㯤యᙧᡂࡀ㉳ࡇࡗ࡚࠸ࡿಶయࡀ࠶ࡿࡇ࡜ࡀ᫂ࡽ࠿࡟࡞ࡗࡓࠋࡲࡓ㸪⾑୰࡜⣅୰ P4 ືែ࡟ᇶ࡙ࡃ༸ᕢ࿘ᮇࡣࡑࢀࡒࢀ24.3s1.3 ᪥㛫࡜ 23.6s1.7 ᪥㛫࡛࠶ࡾ㸪୍⯡ࡢᐙ␆ ࣐࢘ࡢ21s2 ᪥㛫ࡢሗ࿌࡜࡯ࡰྠ⛬ᗘ࡛࠶ࡗࡓࠋ 㞝࡜ྠᒃࡉࡏ࡚࠸࡞࠸≧ែ࡛㣫⫱ࡉࢀ࡚࠸ࡓᮌ᭮㤿No.3 ࡟࠾࠸࡚㸪P4್ࡣ㸪7㹼10 ᭶ࡲ࡛ࡢ3 ࣨ᭶㛫ẚ㍑ⓗ㧗࠸್ࢆ♧ࡋࡓࠋᐙ␆࣐࡛࢘ࡣ㸪୍⯡࡟᤼༸࠿ࡽ⣙ 15 ᪥ᚋ࡟ Ꮚᐑෆ⭷࠿ࡽศἪࡉࢀࡿࣉࣟࢫࢱࢢࣛࣥࢪࣥ㸦PG㸧࡟ࡼࡗ࡚㯤యࡀ㏥⾜ࡋ㸪P4⏘⏕ࡀ

ῶᑡࡍࡿ㸦England, 2005䠅ࠋࡋ࠿ࡋ㸪Stabenfeldt et al.㸦1974㸧ࡣ㸪7 㢌ࡢ㠀ዷፎࡢ࣐࢘

㸦ࢧࣛࣈࣞࢵࢻ㸪ࢡ࢛࣮ࢱ࣮࣮࣍ࢫ㸧࡟࠾࠸࡚ᶵ⬟ⓗ࡞㯤యࡢ⮬↛ᘏ㛗ࢆ☜ㄆࡋ㸪35 㹼95 ᪥㛫 P4ศἪࡀᣢ⥆ࡋࡓࡇ࡜ࢆሗ࿌ࡋ࡚࠸ࡿࠋᮏ◊✲࡟࠾ࡅࡿᮌ᭮㤿No.3 ࡢ 3 ࣨ ᭶㛫ࡢP4ศἪᣢ⥆ࡢཎᅉࡣ୙࡛᫂࠶ࡗࡓࡀ㸪㯤యᶵ⬟ࡢ⮬↛ᘏ㛗ࡀ㉳ࡇࡗ࡚࠸ࡓྍ⬟ ᛶࡀ⪃࠼ࡽࢀࡓࠋ ୍⯡࡟ᐙ␆࣐࢘ࡢዷፎᮇ㛫ࡢ᭱▷ࡣ 310 ᪥㛫࡛㸪᭱㛗ࡀ 370 ᪥㛫࡛࠶ࡿ㸦England, 2005㸧ࠋᮏ◊✲࡟⏝࠸ࡓዷፎᮌ᭮㤿 2 㢌࡛ࡣ㸪᭱⤊஺ᑿ᪥࠿ࡽฟ⏘᪥ࡲ࡛ࡢ᪥ᩘࡣྛࠎ 315 ᪥㛫࡜ 348 ᪥㛫࡛࠶ࡾ㸪ࡑࡢሗ࿌ࡉࢀࡓ⠊ᅖෆ࡛࠶ࡗࡓࠋࡇࢀࡽ 2 㢌ࡢ⣅୰ P4ྵ 㔞ࡣ᭱⤊஺ᑿᚋᚋ 50㹼60 ᪥┠࡟ቑຍࡋጞࡵ㸪120㹼300 ᪥┠࡟㧗࠸್ࢆ⥔ᣢࡋ࡚࠸ࡓ ࡀ㸪ࡑࡢᚋࡣฟ⏘┤๓ࡲ࡛ᛴ⃭࡟ቑຍࡍࡿືែࢆ♧ࡋࡓࠋᐙ␆࣐࡛࢘ࡣዷፎ⣙60 ᪥┠ ௨㝆࡟⫾Ꮚ⫾┙࡟ࡼࡗ࡚ศἪࡉࢀࡿ⾑୰ P4௦ㅰ≀㸦୺࡟ 5α-pregnans㸧ࡢ⃰ᗘࡀᚎࠎ ࡟ቑຍࡋጞࡵ㸪ฟ⏘๓⣙30 ᪥┠࠿ࡽࡉࡽ࡟ᛴ⃭࡟ቑຍࡍࡿ࡜ሗ࿌ࡉࢀ࡚࠸ࡿ㸦Ginther, 1993㸧ࠋᮏ◊✲࡛ほᐹࡉࢀࡓᮌ᭮㤿ࡢዷፎᮇࡢ⣅୰ P4ྵ㔞ࡢኚ໬ࡣ㸪Ginther㸦1993㸧 ࡢሗ࿌࡜ྠᵝ࡞ഴྥࢆ♧ࡋࡓࠋࡲࡓ㸪⣅୰E2࡜E1ྵ㔞ࡣ㸪᭱⤊஺ᑿᚋ⣙90 ᪥┠ࡈࢁ ࠿ࡽᛴ⃭࡟ቑຍࡋጞࡵ㸪ዷፎ୰ᮇ࡟ࣆ࣮ࢡࢆ♧ࡋ㸪ࡑࢀ௨㝆ᚎࠎ࡟ῶᑡഴྥࢆ♧ࡋࡓࠋ ࢧࣛࣈࣞࢵࢻࡸ࢔ࣛࣈရ✀࡛ࡣ㸪⾑୰࢚ࢫࢺࣟࢪ࢙ࣥࡣዷፎ⣙80㹼90 ᪥┠࠿ࡽ᫂☜࡟ ୖ᪼ࡋ㸪210㹼240 ᪥┠ࡈࢁ࡟ࣆ࣮ࢡ್ࢆ♧ࡋ㸪ࡑࢀ௨ᚋฟ⏘ࡲ࡛ῶᑡࡍࡿ࡜ሗ࿌ࡉࢀ

30 ࡚࠸ࡿ㸦Nett et al., 1973㸧ࠋࡉࡽ࡟㸪ࡇࢀࡽࡢ࢚ࢫࢺࣟࢪ࢙ࣥࡣ⫾┙࡟ࡼࡗ࡚ศἪࡉࢀ ࡿࡇ࡜ࡀ᫂ࡽ࠿࡟࡞ࡗ࡚࠸ࡿ㸦Ginther, 1993㸧ࠋࡇࢀࡽࡢࡇ࡜࠿ࡽ㸪ᮌ᭮㤿ࡢዷፎᮇࡢ ⣅୰ P4㸪E2࠾ࡼࡧ E1ྵ㔞ࡢ ᐃࡣ㸪ዷፎෆศἪ≧ែࢆᢕᥱࡍࡿࡇ࡜ࡀ࡛ࡁ㸪ࡉࡽ࡟ ⫾┙ᶵ⬟ࢆᢕᥱࡍࡿࡇ࡜ࡀ࡛ࡁࡿྍ⬟ᛶࡶ⪃࠼ࡽࢀࡓࠋ ᮏ◊✲࡟࠾࠸࡚㸪⣅ࢆ⏝࠸࡚ᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥྵ㔞ࢆ ᐃࡍࡿ㝿࡟ࡣ㸪᤼ἥ┤ ᚋ࠿ࡽ6 ᫬㛫௨ෆ࡟⣅ࢆ᥇ྲྀࡋ㸪෾⤖ಖᏑࡋ࡞ࡅࢀࡤ㸪ṇ☜࡞ ᐃ್ࡀᚓࡽࢀ࡞࠸ࠋ ࡲࡓ㸪ᮌ᭮㤿ࡢ⣅୰P4௦ㅰ≀ྵ㔞ࡢ ᐃ࡟ࡼࡾ㸪༸ᕢ࿘ᮇ㸦sSD㸧ࡣ 23.6s1.7 ᪥㛫 ࡛࠶ࡾ㸪㏻ᖖࡢ⦾ṪᏘ⠇ࡣ5㹼11 ᭶࡛࠶ࡗࡓࡀ㸪࡞࠿࡟ࡣ࿘ᖺ⦾Ṫࡀྍ⬟࡜ᛮࢃࢀࡿ ಶయࡶᏑᅾࡍࡿࡇ࡜ࡀ᫂ࡽ࠿࡜࡞ࡗࡓࠋࡉࡽ࡟㸪ዷፎᮇࡢ⣅୰ P4㸪E2࠾ࡼࡧ E1ྵ㔞 ࡢ ᐃࡣ㸪ዷፎෆศἪ≧ែࡢᢕᥱ࡟᭷ຠ࡛࠶ࡾ㸪⫾┙ᶵ⬟ࢆᢕᥱࡍࡿࡇ࡜ࡀ࡛ࡁࡿྍ ⬟ᛶࡶ⪃࠼ࡽࢀࡓࠋ

32 ๓㡫ᅗࡢㄝ᫂ᩥ㸹 ᅗ1 ᮌ᭮㤿ࡢ㞤 10 㢌ࡢ┤⭠࠿ࡽ᥇ྲྀࡋࡓ⣅୰ࡢࣉࣟࢪ࢙ࢫࢸࣟࣥ㸪࢚ࢫࢺࣛࢪ࣮࢜ ࣝ-17β ࠾ࡼࡧ࢚ࢫࢺࣟࣥྵ㔞ࡢᖹᆒ್㸦sᶆ‽ㄗᕪ㸧ࡢ⤒᫬ⓗኚ໬ 㯤యᮇ㸦 ˈn㸻6㸧࡜㠀㯤యᮇ㸦 ˈn㸻4㸧ࡣ⣅୰ࣉࣟࢪ࢙ࢫࢸࣟࣥྵ㔞࡟ࡼࡾỴ ᐃࡋࡓࠋ *ࡣ┤⭠⣅᥇ྲྀ┤ᚋࡢ 0 ᫬㛫ࡢ್࡜ẚ㍑ࡋ࡚⤫ィⓗ࡟᭷ពᕪ㸦Tukey Kramer ἲ㸸 *p<0.05㸪**p<0.01㸧ࡀ࠶ࡗࡓࡇ࡜ࢆ♧ࡍࠋ

33

ᅗ2 ᮌ᭮㤿ࡢ 4 㢌㸦No.1㹼4㸧ࡢ୍Ⓨ᝟࿘ᮇ୰ࡢࣉࣟࢪ࢙ࢫࢸࣟࣥ࡟࠾ࡅࡿ⾑୰⃰ᗘ

㸦 㸧࡜⣅୰ྵ㔞㸦  㸧ࡢᖹᆒ㸦sᶆ‽ㄗᕪ㸧ືែࡢẚ㍑

34

ᅗ3 ᮌ᭮㤿ࡢ㞤 6 㢌࡟࠾ࡅࡿ⣅୰࡜⾑୰ࡢࣉࣟࢪ࢙ࢫࢸࣟࣥࡢᖺ㛫ືែ

35

ᅗ4 ᮌ᭮㤿 No.7 ࡢዷፎᮇ࡟࠾ࡅࡿ⣅୰ࣉࣟࢪ࢙ࢫࢸࣟࣥ㸪࢚ࢫࢺࣛࢪ࣮࢜ࣝ-17β

࠾ࡼࡧ࢚ࢫࢺࣟࣥྵ㔞ࡢືែ

36

ᅗ5 ᮌ᭮㤿 No.8 ࡢዷፎᮇ࡟࠾ࡅࡿ⣅୰ࣉࣟࢪ࢙ࢫࢸࣟࣥ㸪࢚ࢫࢺࣛࢪ࣮࢜ࣝ-17β

࠾ࡼࡧ࢚ࢫࢺࣟࣥྵ㔞ࡢືែ

37 ➨ ➨ϩ❶ ᕼᑡ࣐࢘⛉ 3 ✀࡟࠾ࡅࡿ⣅୰ࡢᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥࡢ㛗ᮇⓗࣔࢽࢱࣜࣥࢢ 2㸫1 ㅖ ゝ 㔝⏕ࡢࣔ࢘ࢥࣀ࣐࢘ࡣ 19 ୡ⣖୰ᮇ࡟୍ᗘ⤯⁛ࡋࡓ㸪ࡑࡢᚋࡢື≀ᅬ࡛⏕ࡁṧࡗࡓ ࢃࡎ࠿ࡢಶయ⩌ࡢ㣫⫱ୗ⦾Ṫ࡟ࡼࡗ࡚㸪ಶయᩘࡀ኱ᖜ࡟ቑຍࡋࡓࠋ⌧ᅾ෌㔝⏕໬࡟ࡶ ᡂຌࡋ࡚࠸ࡿࡀ㸪౫↛࡜ࡋ࡚⤯⁛༴᝹✀IB 㢮࡟ᣦᐃࡉࢀ࡚࠸ࡿ㸦WAZA, 2006㸧ࠋࢯ࣐ ࣜࣀࣟࣂ࡟࠾࠸࡚ࡣ㣗⏝ࡸ⸆⏝ࡢࡓࡵࡢ⋓ᤕࡸᐙ␆࡜ࡢ➇த࡟ࡼࡾ⃭ῶࡋ㸪㔝⏕໬࡛ ࡣ2002 ᖺ࡟⣙ 600 㢌௨ୗ࡜᥎ᐃࡉࢀ࡚࠸ࡿ㸦IUCN, 2014a㸧ࠋࡲࡓ㸪㣫⫱ୗ࡟࠾࠸࡚ࡶ 2013 ᖺࡲ࡛࡟ 220 㢌ࡋ࠿࠸࡞࠿ࡗࡓ㸦Pohle, 2013㸧ࠋࡉࡽ࡟㸪ࢢࣞࣅ࣮ࢩ࣐࣐࢘࡟࠾ ࠸࡚ࡣ㸪⌧ᅾ࢚ࢳ࢜ࣆ࢔࡜ࢣࢽ࢔࡛ࡢ⏕ᜥࡀ☜ㄆࡉࢀ࡚࠸ࡿ㸦IUCN, 2014c㸧ࠋࡇࢀࡽ ࡢ2 ᅜ࡛ 1970 ᖺ௦࠿ࡽἲⓗ࡟ಖㆤࡉࢀ࡚࠸ࡿࡀ㸪⊁⊟㸪ᐙ␆࡜ࡢ➇த㸪⏕ᜥ⎔ቃࡢᝏ ໬࡞࡝ࡢཎᅉ࡟ࡼࡾಶయᩘࡀ⥅⥆ⓗ࡟ῶᑡࡋࡓ㸦Moehlman, 2002㸧ࠋ࢚ࢳ࢜ࣆ࢔࡛ࡣ 1988 ᖺࡢ⣙ 4276 㢌࠿ࡽ㸪2006 ᖺࡢ⣙ 1468㹼2135 㢌ࡲ࡛㸪18 ᖺ㛫࡛ 50㹼66㸣ῶᑡࡋ ࡓࠋࢣࢽ࢔࡛ࡣ1980 ᖺࡢ⣙ 1900 㢌࠿ࡽ㸪2003 ᖺࡢ⣙ 106 㢌ࡲ࡛㸪23 ᖺ㛫࡛ 94㸣ῶ ᑡࡋࡓ㸦Moehlman, 2002㸧ࠋ⌧ᅾ㸪ࡇࢀࡽࡢ 3 ✀ࡣ⤯⁛ࡢ༴㝤ᛶࡀᴟࡵ࡚㧗࠸࡜ࡉࢀ㸪 ✀ࡢಖᏑ࡜㔝⏕ᅇ᚟ࢆ┠ⓗ࡜ࡋࡓ㣫⫱ୗ⦾Ṫࡢಁ㐍ࡀồࡵࡽࢀ࡚࠸ࡿࠋ ୍᪉㸪࣐࢘⛉ᕼᑡ✀ࡢ㣫⫱ୗ⦾Ṫࢆಁ㐍ࡍࡿࡓࡵ࡟㸪ྛಶయࡢ⦾Ṫ࡟㛵ࢃࡿ⏕⌮≧ ែࢆヲ⣽࡟ᢕᥱࡋ࡚࠾ࡃࡇ࡜ࡀᚲせ࡜࡞ࡾ㸪᪩ᮇዷፎุᐃ࡜ฟ⏘᫬ᮇࡢண ࡣ㸪ಶయ ⟶⌮㸪ฟ⏘๓ࡢ‽ഛࡸḟࡢ⦾Ṫィ⏬ࢆ❧࡚ࡿࡇ࡜࡟ᑐࡋ࡚ᴟࡵ࡚㔜せ࡛࠶ࡿࠋࡉࡽ࡟ˈ 㑇ఏⓗከᵝᛶࢆ㓄៖ࡋ࡚㸪㣫⫱ࢫ࣮࣌ࢫࡢၥ㢟ࡸ㍺㏦࡟࠿࠿ࡿࣜࢫࢡࢆ㑊ࡅࡿࡓࡵ㸪 ேᕤ⦾Ṫᢏ⾡ࡢᛂ⏝࠾ࡼࡧ㛤Ⓨࡀ㐍ࡵࡽࢀ࡚࠸ࡿ㸦Oliver et al., 1982㸧ࠋࡇࢀࡽࡢேᕤ ⦾Ṫᢏ⾡ࡢ฼⏝࡟ࡼࡗ࡚⦾Ṫᡂຌ⋡ࢆྥୖࡉࡏࡿ࡟ࡣ㸪⦾ṪෆศἪᏛⓗ࡞≉ᛶࡢゎ᫂ ࠾ࡼࡧ▱ぢࡢ⵳✚ࡀ୙ྍḞ࡛࠶ࡿࠋ ື≀ࡢ⦾Ṫ⏕⌮ࡢࣔࢽࢱࣜࣥࢢ࡟ࡣ㸪⾑୰ࡢᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ⃰ᗘࡢ ᐃࡀ୍ ⯡ⓗ࡞᪉ἲ࡛࠶ࡿࠋࡋ࠿ࡋ㸪ᐃᮇⓗ࠿ࡘ㢖⦾࡞᥇⾑ࡣᑐ㇟㤿࡟ᑐࡋ࡚ᗄศࡢࢫࢺࣞࢫ

38

ࢆ୚࠼ࡿࡤ࠿ࡾ࠿᥇⾑⪅࡟ࡶᑡ࡞࠿ࡽࡎ༴㝤ࡀక࠺ࠋࡲࡓ㸪㠀౵くⓗ࡞᪉ἲ࡛࠶ࡿ㸪 ᒀ୰࢚ࢫࢺࣟࢪ࢙ࣥࡢ ᐃࡣᐙ␆ࡢ࣐࢘࡜ࣔ࢘ࢥࣀ࣐࡛࢘ࡣ༸ᕢ࿘ᮇࡸዷፎᮇࡢࣔࢽ

ࢱࣜࣥࢢ࡟᭷⏝࡛࠶ࡿࡇ࡜ࡀሗ࿌ࡉࢀ࡚࠸ࡿ㸦Raeside and Liptrap, 1975; Daels et al.,

1991; Monfort et al., 1991㸧ࠋࡋ࠿ࡋ㸪ᒀࡢ᥇ྲྀࡣ⯋ෆࡢᗋᮦࡀ྾Ỉᛶࡢ࡞࠸ሙྜ࡟㝈ࡽ ࢀ㸪ᨺ㣫ሙ࡛ࡢ᥇ྲྀࡣ࡯ࡰ୙ྍ⬟࡛࠶ࡿࠋࡑࡢࡓࡵ㸪ᐜ᫆࡟᥇ྲྀ࡛ࡁࡿ⣅ࢆ⏝࠸ࡓ࣍ ࣝࣔࣥ ᐃἲࡀᗈࡃ౑ࢃࢀࡿࡼ࠺࡟࡞ࡗ࡚࠸ࡿࠋ୍᪉㸪࡯࡜ࢇ࡝ࡢ࣐࢘⛉ື≀ࡣᏘ⠇ ⦾Ṫື≀࡛࠶ࡾ㸪㣫⫱ᆅᇦࡢኚ໬࡟ࡼࡗ࡚⦾ṪᏘ⠇ᛶ࡟ࡶᙳ㡪ࢆཬࡰࡍࡇ࡜ࡀ▱ࡽࢀ ࡚࠸ࡿࠋࡉࡽ࡟㸪Ḣ⡿࡛㣫⫱ࡉࢀ࡚࠸ࡿࣔ࢘ࢥࣀ࣐࢘㸦Schwarzenberger et al., 1992; Scheibe, 1999㸧㸪ࢢࣞࣅ࣮ࢩ࣐࣐࢘㸦Asa et al., 2001㸧࡟࠾࠸࡚⣅୰ࡢᛶࢫࢸࣟ࢖ࢻ࣍ࣝ ࣔࣥ ᐃ࡟ࡼࡿ⦾Ṫ⏕⌮࡟㛵ࡍࡿ㝈ࡽࢀࡓሗ࿌ࡀ࠶ࡿࡀ㸪᪥ᮏ࡟㣫⫱ࡉࢀ࡚࠸ࡿࣔ࢘ ࢥࣀ࣐࢘㸪ࢯ࣐ࣜࣀࣟࣂ࠾ࡼࡧࢢࣞࣅ࣮ࢩ࣐࣐࢘࡟࠾࠸࡚㛗ᮇ㛫࡟Ώࡿ⦾Ṫ⏕⌮ㄪᰝ ࡜✀㛫ẚ㍑ࢆ⾜ࡗࡓሗ࿌ࡣぢᙜࡓࡽ࡞࠸ࠋ ᮏ◊✲࡛ࡣ㸪ࣔ࢘ࢥࣀ࣐࢘ࡢ⣅୰ࡢᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥྵ㔞ࡢືែ࡜ᛶ⾜ື࡟ࡘ ࠸࡚ࡢ㛵㐃ᛶࢆㄪ࡭ࡓࠋࡲࡓ㸪ࣔ࢘ࢥࣀ࣐࢘㸪ࢯ࣐ࣜࣀࣟࣂ࠾ࡼࡧࢢࣞࣅ࣮ࢩ࣐࣐࢘ ࡢ⦾ṪෆศἪᏛ≉ᛶࡢヲ⣽࡞ゎ᫂࡜▱ぢࢆ⵳✚ࡍࡿࡓࡵ㸪㛗ᮇ㛫࡟Ώࡗ࡚⣅୰࡟᤼ἥ ࡉࢀࡿᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥྵ㔞ࡢືែࢆ ᐃࡋࡓࠋࡉࡽ࡟㸪⦾ṪᏘ⠇ࡸ᤼༸࿘ᮇ࠾ ࡼࡧዷፎᮇࡢෆศἪືែࡢ✀㛫࡛ࡢ┦㐪Ⅼ࡟ࡘ࠸᳨࡚ウࡋࡓࠋ 2㸫㸫2 ᮦᩱ࠾ࡼࡧ᪉ἲ 2㸫2㸫1 ౪ヨື≀࣭⣅ࡢ᥇ྲྀ࡜ᐃ㔞 ࣔ࢘ࢥࣀ࣐࢘㸪ࢯ࣐ࣜࣀࣟࣂ࠾ࡼࡧࢢࣞࣅ࣮ࢩ࣐࣐࢘ࢆᑐ㇟࡜ࡋࡓࠋࣔ࢘ࢥࣀ࣐࢘ ࡣ㸪༓ⴥᕷື≀බᅬࡢ㞤1 㢌㸦No.1㸹ࢡࣜࢫ㸧࡜ᮾி㒔ከᦶື≀බᅬࡢ㞤 4 㢌㸦No.2㸸 ࢚࣮ࢥ㸪No.3㸸ࢲ࢖࢔ࢼ㸪No.4㸸ࣇ࣮ࣝࣝ㸪No.5㸸ࢧ࣮ࢩࣕ㸧ࡢィ 5 㢌㸪ࢯ࣐ࣜࣀࣟ ࣂࡣ㸪ᶓ὾ᕷ❧㔠ἑື≀ᅬࡢ㞤 1 㢌㸦No.6㸸ࢧࢡࣛ㸧㸪ࢢࣞࣅ࣮ࢩ࣐࣐࢘ࡣ㸪༓ⴥᕷ ື≀බᅬࡢ㞤1 㢌㸦No.7㸸࢚࢝ࢹ㸧㸪ᐩᒣᕷࣇ࢓࣑࣮ࣜࣃ࣮ࢡࡢ㞤 2 㢌㸦No.8㸸࢟ࣕ

39 ࢧࣜࣥ㸪No. 9㸸ࢪࣗࢹ࢕㸧ࡢィ 3 㢌࡛࠶ࡗࡓࠋ࡞࠾ྛ౪ヨື≀ࡢಶయ᝟ሗࢆ⾲ 1 ࡟ ♧ࡋࡓࠋ ⣅ࡣ㸪㐌 2㹼4 ᅇ᥇ྲྀࡋ㸪᤼ἥ┤ᚋࡲࡓࡣኪ㛫ᐷᐊ཰ᐜ୰࡟᤼ἥࡉࢀࡓࡶࡢ࡛እぢ ୖẚ㍑ⓗ᪂㩭࡞ࡶࡢࢆ⩣ᮅ᥇ྲྀࡋࡓࠋ ᐃ᫬ࡲ࡛㸫20Υୗ࡛෾⤖ಖᏑࡋࡓࠋ ⣅୰ࡢᛶࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ௦ㅰ≀ࡢᢳฟࢆ࣓ࢱࣀ࣮ࣝᢳฟἲ࡟ࡼࡾ⾜࠸㸪P4㸪E2㸪 E1࠾ࡼࡧࢸࢫࢺࢫࢸࣟࣥ㸦T㸧ࢆ ᐃࡋࡓ㸦➨Ϩ❶㸧ࠋࡲࡓ㸪T ࡢᶆ‽≀㉁㸪ᢠయ㸪HRP ᶆ㆑T ࡢ౑⏝᫬⃰ᗘ㸪ྛ✀ࢫࢸࣟ࢖ࢻ࡜ࡢ஺ཫ⋡࠾ࡼࡧ⃰ᗘࡢィ⟬࡟౑⏝ࡋࡓᶆ‽᭤ ⥺㸦୍౛㸧ࢆ⾲2㸪3 ࠾ࡼࡧᅗ 1 ࡟♧ࡋࡓࠋ 2㸫㸫2㸫2 እぢኚ໬࡜ᛶ⾜ືࡢほᐹ ࣔ࢘ࢥࣀ࣐࢘1 㢌㸦No.5㸸ࢧ࣮ࢩࣕ㸧࡛ࡣእ㝜㒊ࡢ≧ែ㸦㝜၁㛤㛢㸧㸪㢖ᒀ࠾ࡼࡧ㞝 ࠿ࡽࡢ㏣ᑿ㸪஺ᑿ⾜ື࡞࡝࡟ࡘ࠸࡚㣫⫱ᢸᙜ⪅ࡀほᐹࡋࡓࠋ 2㸫3 ࢹ࣮ࢱศᯒ  ⣅୰ࡢࡍ࡭࡚ࡢ್࣍ࣝࣔࣥࡣ㸪஝⇱⣅1 g ᙜࡓࡾࡢྵ㔞࡜ࡋ࡚ồࡵࡓࠋᮏ❶࡛ࡣ⣅ ୰P4ྵ㔞ࡢኚ໬࡟ࡼࡗ࡚㸪࣐࢘⛉ᕼᑡ✀ࡢⓎ᝟࿘ᮇࢆィ⟬ࡋࡓ㸦➨Ϩ❶㸧ࠋAmer et al., 㸦2008㸧ࡢ◊✲࡛ࡣᐙ␆࣐࢘ࡢ⾑୰ P4ࡢ᭱పⅬࢆ♧ࡋࡓ᪥ࡣ᤼༸᪥࡛࠶ࡾ㸪⾑୰࠿ࡽ

⣅୰࡬᤼ฟࡲ࡛ࡢࣛࢢࢱ࢖࣒ࡣ1㹼2 ᪥㛫࡛࠶ࡗࡓ㸦Palme et al., 1997; Amer et al., 2008㸧ࠋ

ࡑࡢࡓࡵ㸪ᮏ◊✲࡛ࡣ⣅୰P4ྵ㔞ࡢ᭱పⅬࢆ♧ࡋࡓࢧࣥࣉࣜࣥࢢ᪥ࢆ᤼༸᪥࡜ண ࡋ㸪 ࣛࢢࢱ࢖࣒ࢆ⪃៖ࡋ࡞࠿ࡗࡓࠋࡲࡓ㸪ࣔ࢘ࢥࣀ࣐࢘ࡢዷፎᮇ㛫ࡣ᭱ᚋࡢ஺ᑿ᪥࠿ࡽฟ ⏘᪥ࡲ࡛࡜ᐃࡵࡽࢀࡓࡀ㸪ࢯ࣐ࣜࣀࣟࣂ࡟࠾࠸࡚஺ᑿࡀほᐹࡉࢀ࡞࠿ࡗࡓࡓࡵ㸪⣅୰ P4ྵ㔞ࡢ᭱పⅬࢆ♧ࡋࡓࢧࣥࣉࣜࣥࢢ᪥ࢆ᤼༸᪥㸦஺ᑿ᪥㸧࡜ண ࡋ㸪ண ஺ᑿ᪥࠿ ࡽṚ⏘᪥ࡲ࡛ࢆዷፎᮇ㛫࡜ࡋࡓࠋࡉࡽ࡟㸪ฟ⏘ᚋࡢ⣅୰P4ྵ㔞ࡢ᭱ึࡢ࿘ᮇⓗ࡞ኚື ࡀ㛤ጞࡍࡿ๓ࡢ᭱పⅬࢆ♧ࡋࡓࢧࣥࣉࣜࣥࢢ᪥ࢆⓎ᝟ᅇᖐ᪥࡜ࡋࡓࠋ ࣔ࢘ࢥࣀ࣐࢘ࡢዷፎᮇ㸦3 ᅇ㸧ࡢᖹᆒᅗࡢసᡂ࡟ࡣ㸪1 ᅇࡢዷፎᮇ࡟࠾ࡅࡿᛶࢫࢸ

40 ࣟ࢖ࢻ࣍ࣝࣔࣥ ᐃ್ࡢᖹᆒࢆ10 ᪥㛫ࡈ࡜࡟⟬ฟࡋ㸪ࡉࡽ࡟ 3 ᅇࡢᖹᆒࢆ⟬ฟࡋࡓࠋ ࡑࢀࢆ10 ᪥┠ࡢᩘ್ࠕᖹᆒ್sᶆ‽ㄗᕪ㸦SEM㸧ࠖ࡜ࡋ࡚ᅗ♧ࡋࡓࠋ ⾲1 ࣐࢘⛉ᕼᑡ✀ࡢಶయ᝟ሗ ✀ ಶయ␒ྕ ⾑⤫Ⓩ㘓␒ྕ ಶయྡ 㣫⫱᪋タ ᛶู ⏕ᖺ᭶᪥ ⦾ṪṔ ᅜෆ ᅜ㝿 ࣔ࢘ࢥࣀ࣐࢘ No.1 ࢡࣜࢫ ༓ⴥᕷື≀බᅬ ܲ 1999/9/27 ™ No.2 4509 ࢚࣮ࢥ ᮾி㒔ከᦶື≀බᅬ ܲ 2002/8/11 ™ No.3 3970 ࢲ࢖࢔ࢼ ᮾி㒔ከᦶື≀බᅬ ܲ 2004/9/10 ™ No.4 ࣇ࣮ࣝࣝ ᮾி㒔ከᦶື≀බᅬ ܲ 2008/6/1 ™ No.5 2249 ࢧ࣮ࢩࣕ ᮾி㒔ከᦶື≀බᅬ ܲ 1991/1/14 ۑ ࢯ࣐ࣜࣀࣟࣂ No.6 ࢧࢡࣛ ᶓ὾ᕷ❧㔠ἑື≀ᅬ ܲ 2001/8/1 ۑ ࢢࣞࣅ࣮ࢩ࣐࣐࢘ No.7 ࢚࢝ࢹ ༓ⴥᕷື≀බᅬ ܲ 2004/10/3 㸫 No.8 ࢟ࣕࢧࣜࣥ ᐩᒣᕷࣇ࢓࣑࣮ࣜࣃ࣮ࢡ ܲ 1992/6/9 ۑ No.9 ࢪࣗࢹ࢕ ᐩᒣᕷࣇ࢓࣑࣮ࣜࣃ࣮ࢡ ܲ 2003/6/30 ۑ 㸫㸸୙᫂㸪™㸸࡞ࡋ㸪ۑ㸸࠶ࡿ

41 ⾲ 2 㓝⣲ච␿ ᐃ࡟࠾࠸࡚౑⏝ࡋࡓࢸࢫࢺࢫࢸࣟࣥࡢᶆ‽≀㉁㸪ᢠయ࠾ࡼࡧ HRP ᶆ ㆑ࢸࢫࢺࢫࢸࣟࣥࡢ౑⏝᫬⃰ᗘ ࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ⣧ရ ᶆ‽᭤⥺ࡢ⃰ᗘ⠊ᅖ ࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥᢠయ㸦୍ḟᢠయ㸧 HRP ᶆ㆑ࢫࢸࣟ࢖ࢻ࣍ࣝࣔࣥ ౑⏝᫬ࡢ ᭱⤊ᕼ㔘ಸ⋡ 䝔䝇䝖䝇䝔䝻䞁 17β-hydroxyandrost-4-en-3-one 䠄208-08341䠈࿴ග⣧⸆ᕤᴗ䠅 0.098㸦St.1㸧㹼50 ng/ml㸦St.10㸧 anti testosterone-11α-succinate-BSA 䠄ED131䠈䛒䛩䛛〇⸆䝯䝕䜱䜹䝹䠅 200 ୓ಸ testosterone-11α-succinate-HRP 䠄FKA103䠈䝁䝇䝰䝞䜲䜸䠅 100 ୓ಸ ⾲3 ࢸࢫࢺࢫࢸࣟࣥᢠయ࡟࠾ࡅࡿྛ✀ࢫࢸࣟ࢖ࢻ࡜ࡢ஺ཫ⋡ ࢸࢫࢺࢫࢸࣟࣥᢠయ 㸦ED-131㸪࠶ࡍ࠿〇⸆࣓ࢹ࢕࢝ࣝ㸧 ࢫࢸࣟ࢖ࢻ ஺ཫ⋡㸦㸣㸧 testosterone 100  5α-dihydrotestosterone 41.3 androstenedione 2.07 epitestosterone 0.80 androsterone 0.07 deoxycorticosterone 0.04 dehydroepiandrosterone 0.02 progesterone 0.02 etiocholanolone 㸺 0.01 17α-hydroxyprogesterone 㸺 0.01 cortisol 㸺 0.01 corticosterone 㸺 0.01 cortisone 㸺 0.01

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