Characterization, function and expression of ferredoxins and ferredoxin-NADP^+ oxidoreductases in plastids

植物の各種プラスチドに特異的なFdとFNRの性質・

機能そしてその発現

著者 和田 敬四郎

著者別表示 Wada Keishiro

雑誌名 平成7(1995)年度 科学研究費補助金 一般研究(B)  研究成果報告書概要

巻 1993   1995

ページ 2p.

発行年 1997‑03‑03

URL http://doi.org/10.24517/00066658

Creative Commons : 表示 ‑ 非営利 ‑ 改変禁止 http://creativecommons.org/licenses/by‑nc‑nd/3.0/deed.ja

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1995 Fiscal Year Final Research Report Summary

Characterization, function and expression of ferredoxins and ferredoxin-NADP^+ oxidoreductases in plastids

Research Project

Project/Area Number

05454016

Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation Type

Single-year Grants

Research Field

植物⽣理

Research Institution

Kanazawa University

Principal Investigator

WADA Keishiro Kanazawa University Fac. of Sci., Dept. of Biol. Professor, 理学部, 教授 (70028174)

Co-Investigator(Kenkyū-buntansha)

YAMAGUCHI Kazuo Kanazawa University Institute of Gene Res. Professor, 遺伝⼦実験施設, 教授 (00019879)

Project Period (FY)

1993 – 1995

Keywords

plastids / Chloroplasts / Chromoplasts / Ferredoxins / Ferredoxin-NADP^+ oxidoreductases / Nonphotosynthetic function / PCR / cDNA

Research Abstract

We have investigated characterization, function and expression of ferredoxin isoforms and ferredoxin-NADP^+ oxidoreductase isoenzymes specifically expressed in the various plastids in higher plants. We obtained the following results for three years.

1) Purification, sequence determination and comparison of ferredoxin isoforms

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Published: 1997-03-03

Research Products

All Other All Publications (10 results)

URL: https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-05454016/054540161995kenkyu_seika_hokoku_

2) Characterization of leaf-type and root-type of FNRs 3) Distribution and differential expression of FNR isoenzymes 4) cDNA cloning of root-type FNR of spinach

5) Estimation of divergence time of leaf-and root-type FNRs 6) Function of these isoforms in nonphotosynthetic tissues

[Publications] Morigasaki,S.et al.: "Comparative studied on ferredoxin-NADP^+ oxidoreductase isoenzymes derived from different organs by

antibodies ・・・・・・" Plant Physiology. 103. 435-440 (1993) 

[Publications] Jin,T.et al.: "Purification and characterization of two ferredoxin-NADP^+ oxidoreductase isoforms the first foliage leaves of mung bean

(Vigna radiate) seedlings." Plant Physiology. 106. 697-702 (1994) 

[Publications] Sakai,H.et al.: "Amino acid sequences of ferredoxin isoproteins from Japanese taro (Colocasia esculenta Schott)." J.Plant Research.

107. 299-305 (1994) 

[Publications] Kamide,K.et al.: "Amino acid sequences of heterotrophic and photosynthetic ferredoxins from the tomato plant." Photosynthesis

Research. 46. 301-308 (1995) 

[Publications] Aoki,K.& Wada,K.: "Temporal and spatial distribution of ferredoxin isoproteins in tomato (Lycoperesicon esculentum) fruit." Plant

Physiology. (in press). (1996) 

[Publications] Morigasaki, S.et al.: "Comparative studies on ferredoixn-NADP^+ oxidoreductase isoenzymes derived from different organs by

antibodies specific for the radish root- and leaf-enzymes." Plant Physiol.103. 435-440 (1993) 

[Publications] Jin, T.et al.: "Purification and characterization of two ferredoxin-NADP^+ oxidoreductase isoforms from the first foliage leaves of mung

bean (Bigna ladiata) seedlings." Plant Physiol.106. 697-702 (1994) 

[Publications] Sakai, H.et al.: "Amino acid sequences of ferredoxin isoproteins from Japanese taro (Colocasia esculenta Schott)." J.Plant Res.107.

299-305 (1994) 

[Publications] Kamide, K.et al: "Amino acid sequences of heterotrophic and photosynthetic ferredoxins from the tomato plant." Photosynthesis

Res.46. 301-308 (1995) 

[Publications] Aoki, K.and Wada, K.: "Temporal and spatial destribution of ferredoxin isoproteins in tomato (Lycopersicon esculentum)" Plant Physiol.

(In press). (1996) 