㧙 㧙 41.ઍ⻢ဳࠣ࡞࠲ࡒࡦ㉄ฃኈ II ဳߦࠃࠆࡑ࠙ࠬ༦௯Ᏺ⚦⢩̅㗰☸⚦⢩㑆⋧ᕈࠪ࠽ࡊࠬ㔚ᵹߦኻ ߔࠆᛥᯏ᭴ ⼱ญ⌬↵ޔᬋ⑲ੱ 㜞⍮ᄢቇ㨯කቇㇱ㨯↢ℂቇ㧔⛔ว↢ℂ㧕 㧝㧚⎇ⓥߩ⢛᥊ߣ⋡⊛ 㓶ࡑ࠙ࠬߩࡈࠚࡠࡕࡦߪ㓽ࡑ࠙ࠬߦ⊒ᖱࠍ߽ߚࠄߒޔ❥ᱺߦᔅⷐߥᓎഀࠍᨐߚߒߡࠆޕߒ߆ߒޔ ߎߩലᨐ߇ฃ⢝ߒߡ㑆߽ߥ㓽ߦ⺃ߐࠇࠆߣ㓶ࡈࠚࡠࡕࡦߩ⊒ᖱଦㅴ↪ߦࠃࠅᅧᆼ㒖ᱛ߇↢ߓߡ ߒ߹߁ޕߘߎߢ㓽ࡑ࠙ࠬߪޔ㓶ࡈࠚࡠࡕࡦߦࠃࠆᅧᆼ㒖ᱛࠍ㒐ߋߚߦޔየೝỗࠍᒁ߈㊄ߣߒߡ ㈩㓶ߩࡈࠚࡠࡕࡦࠍ⸥ᙘߒޔߎߩ⸥ᙘߦࠃߞߡᅧᆼࠍ㓚ߒߡࠆޕ ᒰ⎇ⓥቶߢߪߎߩ⸥ᙘߩᐳ߇༦ߢࠅޔઍ⻢ဳࠣ࡞࠲ࡒࡦ㉄ฃኈ 2 ဳ(mGluR2)߇㊀ⷐߥᓎ ഀࠍᨐߚߒߡࠆߎߣࠍⴕേ⮎ℂቇ⊛ᚻᴺߦࠃࠅࠄ߆ߦߒߡ߈ߚޕ༦ߪࡈࠚࡠࡕࡦᖱႎߩᦨೋ ߩਛ⛮ᩭߢࠅޔߎߩㇱߩਥⷐߥ⚻࿁〝ߪ௯Ᏺ⚦⢩г㗰☸⚦⢩㑆ߩ⋧ᕈࠪ࠽ࡊࠬߢࠆޕߒ߆ߒ ߥ߇ࠄޔߎߩ⋧ᕈࠪ࠽ࡊࠬߩᕈ⾰ߦߟߡߪਇߥὐ߇ᄙޕߘߎߢᚒޘߪࡑ࠙ࠬ༦ߩࠬࠗ ࠬᮡᧄࠍߒޔࠣ࡞࠲ࡒࡦ㉄ฃኈേ⮎߅ࠃ߮㒖ኂ⮎߇⋧ᕈࠪ࠽ࡊࠬ㔚ᵹߦ߷ߔലᨐࠍ patch-clamp ᴺࠍ↪ߡ⺞ߴߚޕ 㧞㧚ᣇᴺ ታ㛎ߦߪ Balb/c ࡑ࠙ࠬ(23ޯ35 ᣣ㦂)߆ࠄᚑߒߚෘߐ 250 μm ߩ༦ಾ ࠍ↪ߚޕ௯Ᏺ⚦⢩ ߅ࠃ߮㗰☸⚦⢩߆ࠄߩᔕ╵ߪޔᏱᅃ⊛߽ߒߊߪ nystatin ⓫ሹࡄ࠶࠴ߦࠃࠆ whole-cell clamp ᴺࠍ↪ ߡ⚦⢩߆ࠄ⸥㍳ߒߚޕฦೝỗ‛⾰ߦኻߔࠆᔕ╵ߪޔ⤑㔚ᵹ࿕ቯਅ߹ߚߪ⤑㔚࿕ቯਅ (ᜬ㔚-70 mV) ߢ᷹ቯߒߚޕᔕ╵ߩ୯ߪޔᐔဋ୯rᮡḰ⺋Ꮕߣߒߡߒߚޕ
㧟㧚⚿ᨐ߅ࠃ߮⠨ኤ
௯Ᏺ⚦⢩ߦ⣕ಽᭂᕈߩ㔚ೝỗਈ߃ࠆߣޔ⸥⋧ᕈࠪ࠽ࡊࠬ↱᧪ߩᛥᕈࠪ࠽ࡊࠬᓟ㔚ᵹ(IPSC) ߇↢ߓࠆޕmGluR2 േ⮎㧔DCG-IV㧕߅ࠃ߮㒖ኂ⮎㧔LY341495㧕ߩ⋧ᕈࠪ࠽ࡊࠬ㔚ᵹ㧔IPSC㧕ߦ ኻߔࠆലᨐࠍ⺞ߴߚߣߎࠈޔDCG-IV ߩ⚦⢩ᄖᛩਈߦࠃࠅ IPSC ߪ㗼⪺ߦᛥߐࠇޔLY341495 ߩ⚦⢩ ᄖᛩਈߦࠃࠅჇടߒߚޕߎࠇࠄ DCG-IV ߅ࠃ߮ LY341495 ߩ⋧ᕈࠪ࠽ࡊࠬ㔚ᵹߦኻߔࠆലᨐߪޔ mGluR2 ㆮવሶߩࡁ࠶ࠢࠕ࠙࠻ߦࠃࠅޔߘࠇߙࠇ㒖ኂߐࠇߚޕ ᰴߦޔᵴേ㔚ߩ⊒↢ߦߞߡ↢ߓࠆ IPSP㧔㔚ೝỗߢ↢ߓࠆ IPSC ࠃࠅ߽↢ℂ⊛ᔕ╵ࠍᤋߔࠆ ߣ⠨߃ࠄࠇࠆ㧕ߦኻߔࠆ LY341495 ߩലᨐࠍ⺞ߴߚߣߎࠈޔIPSP ߪჇᄢߒߚޕ 㧠㧚߹ߣ ᧄ⎇ⓥ⚿ᨐ߆ࠄޔᵴേ㔚ߩ⊒↢ߦࠃࠅߐࠇࠆౝ࿃ᕈࠣ࡞࠲ࡒࡦ㉄ߦࠃߞߡ mGluR2 ߇ᵴᕈൻ ߐࠇޔߘࠇߦࠃߞߡ⋧ᕈࠪ࠽ࡊࠬવ㆐߇ᛥߐࠇࠆߎߣ߇␜ໂߐࠇߚޕ
