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An Evolutionarily Conserved Plant RKD Factor Controls Germ Cell Differentiation

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(1)85. A. RKD. MpRKD PpRKD AtRKD5 MdRKD GmRKD1 GmRKD8 99 AtRKD4. 84 65. 99 85. 63. 65 75 98. 56. TaRKD2 TaRKD1 AtRKD3 AtRKD1 AtRKD2 93 GmRKD2 GmRKD7 GmRKD9 GmRKD4 78 GmRKD5 GmRKD3 GmRKD6. NtRKD BdRKD. MpRWP1 PpRWP4 PpRWP3. 99 71. 78. CreMID VcaMID1m PpRWP5 MpRWP2 PpRWP2 PpRWP6. 99 89. 89. 59. GmRWP4 GmRWP5 99 GmRWP3 99 CreNIT2 VcaNIT2 PpNLP7 PpNLP5 PpNLP6 PpNLP4 PpNLP3 61 PpNLP2 PpNLP1 MpNLP 71 AtNLP1 AtNLP2 GmNLP11 71 GmNLP2 69 GmNLP1 GmNLP4 AtNLP5 AtNLP3 65 AtNLP4 GmNLP5 GmNLP3 97 73 AtNLP8 AtNLP9 74 GmNLP8 GmNLP9 73 GmNLP10 GmNLP12 95 90 GmNLP14 GmNLP7p AtNLP6 61 AtNLP7 GmNLP13 59 GmNLP6 87. NLP. 87. 97. DdiRWPRK1 DdiRWPRK2. 76. 0.2. B M. polymorpha RKD P. patens RKD A. thaliana RKDs. Figure 1. 586 aa 572 aa 256-331 aa.

(2) A ♂ Antheridium development SCs SMC Jacket layer. Sperm. Antheridiophore. B ♀ Archegonium development Egg VCC Wall Cell Archegoniophore. C. NCC. Mature egg. CVC. D. E. Egg. Egg. Zygote. VCC NCC. F. G. H. SCs. I. J. K. L. M Egg. Egg. VCC. Figure 2.

(3) A. Egg. B. Egg. Egg. NCC. D Egg. VCC. VCC. VCC. NCC. C. NCC. WT ♀. E. Egg. F. G. H Aborted egg. Egg. VCC. Egg. VCC. VCC NCC. NCC. NCC. Mprkd-1. I. J. Egg. M. Egg. Egg. VCC VCC. WT ♀. K. WT ♀. N. L Vacuole. VCC. Mprkd-2. Figure 3. VCC. Mprkd-2.

(4) A. B. WT. C. WT. D. * * * *. Mprkd-3. E. F. Mprkd-3 Figure 4. Mprkd-3. Mprkd-3.

(5)

Figure 1  A  B  P. patens RKD  572 aa 586 aa A. thaliana RKDs  256-331 aa M. polymorpha RKD NLP RKD  MpRKD PpRKD AtRKD5 MdRKD GmRKD1 GmRKD8 AtRKD4 TaRKD2 TaRKD1 AtRKD3 AtRKD1 AtRKD2 GmRKD2 GmRKD7 GmRKD9 GmRKD4 GmRKD5 GmRKD3 GmRKD6 NtRKD BdRKD MpRWP1 PpRWP4

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