㻔㻞㻢㻕 㮵ඣᓥ⣧ᚰዪᏊᏛ┳ㆤᰤ㣴Ꮫ㒊⣖せ䚷㻌㼂㼛㼘㻚㻞㻜 䠖 㻞㻢㻌䡚㻌㻟㻝㻘㻌㻞㻜㻝㻢
ডৗ઼ً͈ρΛΠ͈ুอ൲͂ະհ࣐൲̤̫ͥͅ
൲̹̩ͭ͋ৗ̹̩͂ͭ͋ৗ͈ڛ
ါȁȁ
࠙┠ⓗࠚ⬡㉁㐣㣗㸦㻴㻲㻰㸧࠾ࡅࡿື≀ᛶ᳜≀ᛶࡓࢇࡥࡃ㉁ࡀࣛࢵࢺࡢ⮬Ⓨⓗ࡞㍯ᅇࡋ⾜ື
Ᏻ⾜ືཬࡰࡍᙳ㡪ࡘ࠸࡚㸪⬡㉁ࡢ㉁ࡢ㛵㐃ᛶࢆ᳨ウࡋࡓࠋ
࠙᪉ἲࠚ㻠 㐌㱋ࡢ 㻿㼜㼞㼍㼓㼡㼑㻙㻰㼍㼣㼘㼑㼥 ⣔㞤ࣛࢵࢺࢆྛ㣗㣵⩌↓సⅭศࡅ࡚ᐇ㦂㣫ᩱ࡛ 㻝㻞 㐌㛫㣫⫱ࡋ ࡓࠋᐇ㦂㣫ᩱࡣື≀ᛶࡓࢇࡥࡃ㉁ࡋ࡚࢝ࢮࣥ㸪᳜≀ᛶࡓࢇࡥࡃ㉁ࡋ࡚㇋ࡓࢇࡥࡃ㸪⬡㉁ࡣ ࡑࢀࡒࢀࡢࡓࢇࡥࡃ㉁⩌ࢆ 㼚㻙㻥 ⣔࢜ࣞࣥ㓟ࢆᡂศࡍࡿ࣮࢜ࣜࣈἜ 㼚㻙㻟 ⣔Ș 㻙 ࣜࣀࣞࣥ㓟ࢆ
ᡂศࡍࡿ࢚ࢦ࣐Ἔࢆ⏝ࡋ㸪ࡑࡢࡢᡂศࡣࡍ࡚ྠࡌ࡛⾜ࡗࡓࠋ⾜ືᐇ㦂ࡣᅇ㌿ᘧ㐠ື ᐃ
⨨࠾ࡼࡧ㧗ᯫᘧ༑Ꮠ㏞㊰ヨ㦂ࢆ⏝࠸࡚ࡑࢀࡒࢀ⮬Ⓨ㐠ືᏳ⾜ືࢆほᐹࡋࡓࠋ
࠙⤖ᯝࠚྛ㣗㣵⩌㛫ࡢᦤྲྀ࣮࢝ࣟࣜ㔞ࢆྠ୍ࡋࡓ㸪㍯ᅇࡋ⾜ືࡼࡿ⮬Ⓨ㐠ື࡛ࡣ㸪࢝ࢮࣥ
㇋ࡓࢇࡥࡃࡶ࢚ࢦ࣐Ἔࡢ⤌ࡳྜࢃࡏ࠾࠸࡚ 㻴㻲㻰 ࡢ᪉ࡀᶆ‽㣗ࡼࡾࡶከ࠸ഴྥࡀࡳࡽࢀ㸪
≉㇋ࡓࢇࡥࡃ㉁࡛㢧ⴭ࡛࠶ࡗࡓࠋ㻴㻲㻰 ࠾ࡅࡿᏳ⾜ື࡛ࡣ㸪࢝ࢮࣥ㇋ࡓࢇࡥࡃ㉁ࡢ ᙳ㡪ࡼࡾࡶ㸪㣗ྜࢃࡏࡢ⬡㉁ࡢ㉁ࡼࡗ࡚ኚືࡍࡿࡇࡀㄆࡵࡽࢀࡓࠋ㻴㻲㻰 ࢆᶆ‽㣗ྠࡌ࢝
࣮ࣟࣜ㔞࡛ᦤྲྀࡍࡿ㸪࢝ࢮࣥ㇋ࡓࢇࡥࡃ㉁ࡶ࢚ࢦ࣐Ἔࡢ᪉ࡀ࣮࢜ࣜࣈἜࡼࡾ࣮࢜ࣉࣥ
ᮎ➃㡿ᇦࡢᅾ㛫ࡀ㛗ࡗࡓࡢ㸪㧨ࡢືࡁࡽᏳឤࡀᑡ࡞࠸⾜ື࡛࠶ࡗࡓࠋ
࠙⪃ᐹࠚື≀ᛶࡓࢇࡥࡃ㉁ࡸ᳜≀ᛶࡓࢇࡥࡃ㉁ࡣྠᦤྲྀࡍࡿ⬡㉁ࡢ㉁ࡼࡗ࡚⮬Ⓨ㐠ືࡸᏳ
⾜ືࡀ␗࡞ࡿࡇࡀ᫂ࡽ࡞ࡗࡓࠋ≉Ᏻ⾜ື࡛ࡣࡓࢇࡥࡃ㉁ࡼࡾࡶ⬡㉁ࡢ㉁ࡢᙳ㡪ࡀࡁ࠸
ࡇࡀㄆࡵࡽࢀࡓࠋ ࠉ
࣮࣮࢟࣡ࢻ㸸⬡㉁㐣㣗㸭 +LJK)DW'LHW⮬Ⓨ㐠ື㸪㍯ᅇࡋ㐠ື㸪Ᏻ⾜ື
ȁ࡞
ඛ⾜◊✲࠾࠸࡚ⴭ⪅➼ࡣ⬡㉁ࡢ㉁ࡢ㐪࠸ࡼࡾ
Ᏻ⾜ືࡀ␗࡞ࡿࡇࢆ᫂ࡽࡋࡓ
㻝㻘㻞㻘㻟㸧ࠋࡋࡋ
࡞ࡀࡽ㸪ྠᦤྲྀࡢࡓࢇࡥࡃ㉁⬡㉁ࡢ┦స⏝
ࡀ⾜ືཬࡰࡍᙳ㡪ࡘ࠸࡚ࡣᮍࡔ᫂ࡽ࡛ࡣ࡞
࠸ࠋ㣗≀⏤᮶ࡢࡓࢇࡥࡃ㉁ࡣᑠ㯏ࡢࢢࣝࢸࣥ㸪࣑
ࣝࢡࡢ࢝ࢮࣥࡸ㇋ࡢࢯࣇࣛ࣎ࣥ࡞࢜ࣆ࢜
ࢻཷᐜయࡢࣜ࢞ࣥࢻ࡞ࡿ࣌ࣉࢳࢻࡀྵࡲࢀ࡚࠸ࡿ
ࡇࡀ᫂ࡽࡉࢀ࡚ࡁࡓ
㻠㻘㻡㻕㻌ࠋࡑࢀࡽࡢࡓࢇࡥࡃ㉁
ࡣ⤫ྜኻㄪࡸ⮬㛢࡞ࡢື⾜ື
㻢㻘㻣㻕㻌㸪ࡉࡽᨷ ᧁ⾜ື࣭♫ⓗ⾜ື࣭Ꮫ⩦⌮ゎ➼ᙳ㡪ࡀ࠶ࡿሗ
࿌ࡉࢀ࡚࠸ࡿ
㻤㻘㻥㻘㻝㻜㻕㻌ࠋ୍᪉㸪⫧‶ࢆㄏᑟࡍࡿ㣗㣵ࡣ
Ᏻ⾜ືࡸᏛ⩦⌮ゎ㛵ࡋ࡚࠸ࡿࡇࡀ♧၀ࡉࢀ࡚
࠸ࡿ
㻝㻝㻙㻝㻠㻕ࠋᮏ◊✲࡛ࡣ㸪ື≀ᛶ᳜≀ᛶࡓࢇࡥࡃ㉁
ࡀ⬡㉁ࡢ㉁ࡢ㐪࠸ࡢ┦㛵ಀࡼࡾ⮬Ⓨⓗ㐠ືࡸ
Ᏻ⾜ືཬࡰࡍᙳ㡪ࡘ࠸᳨࡚ウࢆ⾜ࡗࡓࠋ
༷ȁ༹
ື≀ᐇ㦂㸸㻠 㐌㱋ࡢ 㻿㼜㼞㼍㼓㼡㼑㻙㻰㼍㼣㼘㼑㼥㻔㻿㻰㻕㻌⣔㞤ࣛࢵࢺ 㸦㻿㻸㻯㸪㟼ᒸ㸧ࢆ 㻤 ⩌ศࡅ㸦㻲㼕㼓㻚㻝㸧 㸪ᐇ㦂㣫ᩱ࡛ 㻝㻞 㐌㛫㣫⫱ࡋࡓࠋ㣫⫱ࡣᐊ 㻞㻡Υ㸪㻝㻞 㛫᫂ᬯ࿘ᮇ
࡛⾜ࡗࡓࠋ
ा֔ȁࠃঊȄਹȁঊ
㮵ඣᓥ⣧ᚰዪᏊᏛ┳ㆤᰤ㣴Ꮫ㒊ᗣᰤ㣴Ꮫ⛉
㻲㼕㼓㻚㻝㻌㻳㼞㼛㼡㼜㼟㻌㼛㼒㻌㼑㼤㼜㼑㼞㼕㼙㼑㼚㼠㻚 䚷䚷䚷㻌㼚㻙㻥㻦㻌㼛㼘㼕㼢㼑㻌㼛㼕㼘㻘㻌㼚㻙㻟㻦㻌㼑㼓㼛㼙㼍㻌㼛㼕㼘㻘 䚷䚷䚷㻌㻺㻦㻌㼚㼛㼞㼙㼍㼘㻌㼐㼕㼑㼠㻘㻌㻴㻲㻦㻌㼔㼕㼓㼔㻌㼒㼍㼠㻌㼐㼕㼑㼠
㼚㻙㻥
㻯㼍㼟㼑㼕㼚 㻿㼛㼥
㻺 㻴㻲
㻺 㻴㻲
㼚㻙㻟
㻯㼍㼟㼑㼕㼚 㻿㼛㼥
㻺 㻴㻲
㻺 㻴㻲
㻔㻞㻣㻕
⬡㉁㐣㣗䛾䝷䝑䝖䛾⮬Ⓨ㐠ື䛸Ᏻ⾜ື䛻䛚䛡䜛ື≀ᛶ䛯䜣䜁䛟㉁䛸᳜≀ᛶ䛯䜣䜁䛟㉁䛾ẚ㍑
ᐇ㦂㣫ᩱ㸸ື≀ᛶࡓࢇࡥࡃ㉁ࡋ࡚࢝ࢮࣥ 㻔㼣㼍㼗㼛㻕㸪
᳜≀ᛶࡓࢇࡥࡃ㉁ࡋ࡚㇋ࡓࢇࡥࡃ㉁ 㻔㼣㼍㼗㼛㻕㻌 ࢆ
⏝ࡋ㸪ࡑࢀࡒࢀࡢࡓࢇࡥࡃ㉁⩌ࢆ 㼚㻙㻥㻌⣔࢜ࣞࣥ㓟
ྵ᭷࣮࢜ࣜࣈἜ㸦㼣㼍㼗㼛㸧 㼚㻙㻟㻌 ⣔ࡢȘ 㻙 ࣜࣀࣞࣥ㓟
ྵ᭷࢚ࢦ࣐Ἔ㸦ᮅ᪥ 㻿㼀㸧ศࡅ㸪᭦ྛ⬡㉁⩌ࢆᶆ
‽㣗㸦㼚㼛㼞㼙㼍㼘㻌 ⬡㉁㐣㣗㸦㻴㻲㻰㸧ศࡅ࡚⾜ࡗ ࡓ㸦㼀㼍㼎㼘㼑㻌㻝㸧 㻌ࠋࡑࡢࡢ㣫ᩱࡢᡂศࡣࢥ࣮ࣥࢫࢱ࣮ࢳ 㸦᪥ᮏ⃦⢊ᰴ㸧 㸪ࢩࣗࢡ࣮ࣟࢫ㸦㼣㼍㼗㼛㸧 㸪ࢭ࣮ࣝࣟࢫ㸦ࢼ
࢝ࣛᰴ㸧 㸪 ࣑ࢿ࣑ࣛࣝࢵࢡࢫ㸦࢚࢜ࣜࣥࢱࣝ㓝ẕᰴ㸧 㸪
ࣅࢱ࣑࣑ࣥࢵࢡࢫ㸦㻿㻸㻯 ᰴ㸧 㸪 ࢥࣜࣥ㸦㼣㼍㼗㼛㸧࡛࠶ࡗࡓࠋ
⢾㉁࣭ࡓࢇࡥࡃ㉁࣭⬡㉁ࡢ࢚ࢿࣝࢠ࣮ 㻑 ࡣᶆ‽㣗ࡀ 㻡㻤㻑 ࣭ 㻝㻥㻑 ࣭ 㻞㻟㻑 ࡋ㸪㻴㻲㻰 ࡣ 㻞㻢㻑 ࣭ 㻞㻥㻑 ࣭ 㻠㻡㻑 ࡛⾜ࡗࡓࠋ ᐇ㦂㣫ᩱࡣྛ⩌ࡶ࣮࢝ࣟࣜ㔞ࢆྠࡌࡋ࡚ᢞࡋ㸪
⩣᪥ṧ㔞ࢆ ᐃࡋ࡚ᦤྲྀ㔞ࢆグ㘓ࡋࡓࠋỈࡣ⮬⏤ᦤ
ྲྀ࡛⾜ࡗࡓࠋ
⾜ືᐇ㦂㸸⮬Ⓨ㐠ືࡣࣛࢵࢺ⏝ᅇ㌿ᘧ㐠ື ᐃ⨨
㸦㍯ᅇࡋ✵㛫㸸┤ᚄ 㻟㻤 㸪 ᖜ 㻥㻚㻡 㸧 㸦㻲㼕㼓㻚㻞㸧 ࢆ⏝ࡋ࡚㸪 㻡 ศ㛫ࡢ⮬Ⓨ㐠ື࠾ࡅࡿᅇ㌿㍯ෆࡢᅾ㛫㸪ᅇ㌿
ᩘ࠾ࡼࡧᅇ㌿㍯๓ᐊ㛫ࡢ⛣ືᅇᩘ㸪ẟ࡙ࡃࢁ࠸
㛫࡞ࡢグ㘓ࢆ⾜ࡗࡓ 㻔㻲㼕㼓㻚㻞㻕ࠋᏳ⾜ືࡣ㧗ᯫᘧ༑
Ꮠ㏞㊰ヨ㦂ࢆ᪤ሗ
㻞㸧ྠᵝ⾜ࡗࡓࠋ
ᑦ㸪ື≀ᐇ㦂ࡣ㮵ඣᓥ⣧ᚰዪᏊᏛື≀ᐇ㦂ᣦ㔪
ᚑࡗ࡚⾜ࢃࢀࡓࠋ
ࠫȁض
⮬Ⓨ㐠ື㸸㍯ᅇࡋ⾜ືࡢᅇ㌿ᩘࡣ㸪࢝ࢮࣥ㇋
ࡓࢇࡥࡃ㉁ࡶ㸪࢚ࢦ࣐Ἔࡢ⤌ࡳྜࢃࡏࡽࡣ 㻴㻲㻰 ࡢ᪉ࡀᶆ‽㣗ࡼࡾࡶከ࠸ഴྥࡀࡳࡽࢀ㸪≉
㇋ࡓࢇࡥࡃ㉁࡛㢧ⴭ࡛࠶ࡗࡓ㸦㻲㼕㼓㻚㻟㻭㻚㸧 ࠋ୍᪉㸪 ࣮࢜ࣜ
㼀㼍㼎㼘㼑㻌㻝ࠉ㻱㼤㼜㼑㼞㼕㼙㼑㼚㼠㼍㼘㻌㻰㼕㼑㼠㼟
㸦㼓㸧㼓㼞㼛㼡㼜 Ϩ ϩ Ϫ ϫ Ϭ ϭ Ϯ ϯ
㼜㼞㼛㼠㼑㼕㼚 㼏㼍㼟㼑㼕㼚 㼏㼍㼟㼑㼕㼚 㼟㼛㼥 㼟㼛㼥 㼏㼍㼟㼑㼕㼚 㼏㼍㼟㼑㼕㼚 㼟㼛㼥 㼟㼛㼥
㼘㼕㼜㼕㼐 㻻㼘㼕㼢㼑
㻺
㻻㼘㼕㼢㼑 㻴㻲㻰
㻻㼘㼕㼢㼑 㻺
㻻㼘㼕㼢㼑 㻴㻲㻰
㻱㼓㼛㼙㼍 㻺
㻱㼓㼛㼙㼍 㻴㻲㻰
㻱㼓㼛㼙㼍㻌 㻺
㻱㼓㼛㼙㼍 㻴㻲㻰
㻯㼛㼞㼚㼟㼠㼍㼞㼏㼔 㻞㻡㻣 㻤㻤 㻞㻡㻣 㻤㻤 㻞㻡㻣 㻤㻤 㻞㻡㻣 㻤㻤
㼏㼍㼟㼑㼕㼚 㻝㻜㻜 㻝㻡㻠 㻜 㻜 㻝㻜㻜 㻝㻡㻠 㻜 㻜
㼟㼛㼥㼎㼑㼍㼚 㻜 㻜 㻝㻜㻜 㻝㻡㻠 㻜 㻜 㻝㻜㻜 㻝㻡㻠
㻻㼘㼕㼢㼑㻌㼛㼕㼘 㻡㻡 㻝㻜㻢 㻡㻡 㻝㻜㻢 㻜 㻜 㻜 㻜
㻱㼓㼛㼙㼍㻌㼛㼕㼘 㻜 㻜 㻜 㻜 㻡㻡 㻝㻜㻢 㻡㻡 㻝㻜㻢㻌
Entrance room
Hight: 15cm, length 38cm, Wide 13cm
Diameter: 38cm Wide: 9.5cm
㻲㼕㼓㻚㻞㻌㻌㻭㼏㼠㼕㼢㼕㼠㼥㻙㼣㼔㼑㼑㼘㼟㻌㼛㼒㻌㼞㼍㼠㼟㻚㻌
A B
30 35
20 140 160
20 25
60 80 100 120
5 10 15
0 20 40
N HF N HF N HF N HF
0 N HF N HF N HF N HF
casein soy casein soy Casein Soy Casein Soy
olive olive egoma egoma
㻲㼕㼓㻚㻟㻌㻌㻭㻦㻌㻿㼜㼛㼚㼠㼍㼚㼑㼛㼡㼟㻌㼍㼏㼠㼕㼢㼕㼠㼥㻌㼛㼚㻌㼣㼔㼑㼑㼘㻌㼞㼡㼚㼚㼕㼚㼓㻌㼕㼚㻌㼞㼍㼠㼟㻌㼒㼑㼐㻌㼑㼍㼏㼔㻌㼑㼤㼜㼑㼞㼕㼙㼑㼚㼠㼍㼘㻌㼐㼕㼑㼠㻚 䚷䚷䚷㻌㻌㻮㻦㻌㼀㼔㼑㻌㼟㼠㼍㼥㼕㼚㼓㻌㼠㼕㼙㼑㻌㼛㼒㻌㼞㼍㼠㼟㻌㼍㼠㻌㼠㼔㼑㻌㼣㼔㼑㼑㼘㻌㼜㼘㼍㼏㼑㻚
olive olive egoma egoma
(cycles) (sec)
㻔㻞㻤㻕 㮵ඣᓥ⣧ᚰዪᏊᏛ┳ㆤᰤ㣴Ꮫ㒊⣖せ䚷㻌㼂㼛㼘㻚㻞㻜㻘㻌㻞㻜㻝㻢
ࣈἜࡢ⤌ࡳྜࢃࡏ࡛ࡣ࢝ࢮࣥ㇋ࡓࢇࡥࡃ
ࡶᶆ‽㣗 㻴㻲㻰 ࡣྠࡌഴྥ࡛ᕪࡣㄆࡵࡽࢀ࡞ࡗࡓࠋ
ࡲࡓ㸪ᅇ㌿㍯ࡢᅾ㛫ࡣ㸪ྛ㣗㣵⩌㛫ᕪࡣㄆࡵ
ࡽࢀ࡞ࡗࡓ㸦㻲㼕㼓㻚㻟㻮㸧 ࠋ
Ᏻ⾜ື㸸㧗ᯫᘧ༑Ꮠ㏞㊰ヨ㦂࡛ࡢ࣮࢜ࣉ࣮࣒ࣥ
ࡢᮎ➃㸦㻱㼐㼓㼑㸧ᮎ➃㏆࠸㡿ᇦ㸦㻭 㡿ᇦ㸧ࡘ࠸
࡚ᅾ㛫㸪ᮎ➃ࡽୗࢆな࠸ࡓᅇᩘ㸪ᮎ➃࡛ࡢ㧨 ࡢືࡁࢆほᐹࡋ㸪Ᏻᵝ⾜ືࡢ ᐃ࣭ほᐹࢆ⾜ࡗࡓ 㸦㻲㼕㼓㻚㻠㸧 ࠋ࣮࢜ࣉ࣮࣒ࣥࡢᮎ➃ࡢᅾ㛫ࡣ㸪࢝ࢮ
ࣥࡢሙྜ 㻴㻲㻰 ࡢ࣮࢜ࣜࣈἜࡣᶆ‽㣗㸦㻺㼛㼞㼙㼍㼘㸸㻺㸧
ࡼࡾ㛗ࡃ㸪㏫࢚ࢦ࣐Ἔࡣ 㻺 ࡼࡾప࠸ഴྥ࡛࠶ࡗࡓ 㸦㻲㼕㼓㻚㻠㻌 Ϩ㸪ϩ㸧 ࠋ㇋ࡓࢇࡥࡃ㉁ࡢሙྜࡣ㸪࣮࢜ࣜࣈ
Ἔ࢚ࢦ࣐Ἔࡶ 㻴㻲㻰 ࡢ᪉ࡀ 㻺 ࡼࡾ㧗࠸ഴྥࢆࡋ
ࡵࡋࡓࠋ࣮࢜ࣉ࣮࣒ࣥࡢ 㻭 㡿ᇦࡢᅾ㛫ࡣ㸪࢝
ࢮࣥࡢሙྜ 㻴㻲㻰 ࡢ࣮࢜ࣜࣈἜࡣ 㻱㼐㼓㼑 ྠࡌࡃᶆ
‽㣗㸦㻺㼛㼞㼙㼍㼘㸸㻺㸧ࡼࡾ㧗࠸ഴྥࢆ♧ࡋࡓ㸦㻲㼕㼓㻚㻠㻌Ϫ㸧 ࠋ
୍᪉㸪࢚ࢦ࣐Ἔ࡛ࡣ 㻴㻲㻰 㻺 ࡣྠࡌࣞ࣋ࣝࢆ♧ࡋ ࡓ 㸦㻲㼕㼓㻚㻠㻌ϫ㸧 ࠋ㇋ࡓࢇࡥࡃ㉁ࡢሙྜࡣ 㻴㻲㻰 ࡢ࣮࢜ࣜ
ࣈἜ࢚ࢦ࣐Ἔࡣ 㻱㼐㼓㼑 ྠࡌࡃ 㻺 ࡼࡾ㧗࠸ഴྥࢆ♧
ࡋࡓࠋࡲࡓ㸪㻱㼐㼓㼑 㻭 㡿ᇦࡢᅾ㛫ࡣ㸪࢝ࢮࣥ
㇋ࡓࢇࡥࡃ㉁ࡢࡑࢀࡒࢀ࠾࠸࡚࢚ࢦ࣐Ἔࡢ᪉ ࡀ࣮࢜ࣜࣈἜࡼࡾࡶ㧗࠸ഴྥࢆ♧ࡋࡓࠋ
ḟᗋࡽ 㻢㻜 ࡢ㧗ࡉ࠶ࡿ࣮࢜ࣉ࣮࣒ࣥࡢᮎ
➃ࡽୗࢆなࡁ㎸ࡴ⾜ື࠾࠸࡚㸪㇋ࡓࢇࡥࡃ㉁
࡛ࡣ࣮࢜ࣜࣈἜ࢚ࢦ࣐Ἔࡶ 㻴㻲㻰 ࡢ᪉ࡀ 㻺 ࡼࡾ
ከ࠸ഴྥࢆ♧ࡋࡓ㸦㻲㼕㼓㻚㻡㸧 ࠋ࢝ࢮ࡛ࣥࡣ࢚ࢦ࣐Ἔࡢ ᪉ࡀ 㻴㻲㻰 ࡸ 㻺 ࡶ࣮࢜ࣜࣈἜࡼࡾࡶ㧗࠸ഴྥࢆ♧
ࡋࡓࠋ
䊠䠊㻻㼘㼕㼢㼑㻌㼛㼕㼘㻌 䊡䠊㻱㼓㼛㼙㼍㻌㼛㼕㼘
3 4 5 6
3 4 5 6
Edge
0 1 2
0 1 2
10
12
(sec) (sec)
(sec) (sec)
6 7 8 9 10
6 8 10
䊢
䊣
1 2 3 4 5
2 4
A area
0 0
Normal HFD Normal HFD
casein casein soy soy Normal HFD Normal HFD casein casein soy soy
㻲㼕㼓㻚㻠䚷㻭㼚㼤㼕㼑㼠㼥㻌㼎㼑㼔㼍㼢㼕㼛㼞㻌㼛㼒㻌㼞㼍㼠㼟㻌㼒㼑㼐㻌㼑㼕㼠㼔㼑㼞㻌㼏㼍㼟㼑㼕㼚㻌㼛㼞㻌㼟㼛㼥㻌㼜㼞㼛㼠㼑㼕㼚㻌㼍㼠㻌㻱㼐㼓㼑㻌䠄䊠䠈䊡䠅 䚷䚷䚷䚷㼍㼚㼐㻌㻭㻌㼍㼞㼑㼍䠄䊢䠈䊣䠅㻌㻌㼎㼥㻌㻱㼘㼑㼢㼍㼠㼑㼐㻌㼜㼘㼡㼟㻌㼙㼍㼦㼑㻌㼠㼑㼟㼠㻚
㻭㻦㻌㻻㼘㼕㼢㼑㻌㼛㼕㼘㻌 㻮㻦㻌㻱㼓㼛㼙㼍㻌㼛㼕㼘
;Ɵŵes) ;Ɵŵes)
6 7 8
6 7
8;Ɵŵes) ;Ɵŵes)
3 4 5
3 4 5
0 1 2
0 1 2
Normal HFD Normal HFD 0 Normal HFD Normal HFD
Casein Casein Soy Soy Casein Casein Soy Soy
㻲㼕㼓㻚㻡㻌㻌㻌㻲㼞㼑㼝㼡㼑㼚㼏㼥㻌㼛㼒㻌㼘㼛㼛㼗㼕㼚㼓㻌㼐㼛㼣㼚㻌㼒㼞㼛㼙㻌㼠㼔㼑㻌㼑㼐㼓㼑㻌㼛㼒㻌㼠㼔㼑㻌㼛㼜㼑㼚㻌㼍㼞㼙㻌㼍㼠㻌㻌
㻌㻌㻌㻌㻌㻌㻌㻌㻌㻌㻌㻌㻱㼘㼑㼢㼍㼠㼑㼐㻌㼜㼘㼡㼟㻌㼙㼍㼦㼑㻌㼠㼑㼟㼠㻚㻌㻭㻦㻌㼛㼘㼕㼢㼑㻌㼛㼕㼘㻌㼓㼞㼛㼡㼜㼟㻘㻌㻮㻦㻌㼑㼓㼛㼙㼍㻌㼛㼕㼘㻌㼓㼞㼛㼡㼜㼟㻚
㻔㻞㻥㻕
⬡㉁㐣㣗䛾䝷䝑䝖䛾⮬Ⓨ㐠ື䛸Ᏻ⾜ື䛻䛚䛡䜛ື≀ᛶ䛯䜣䜁䛟㉁䛸᳜≀ᛶ䛯䜣䜁䛟㉁䛾ẚ㍑
ࣉȁख़
ࡓࢇࡥࡃ㉁ࡢື≀ᛶ᳜≀ᛶࡢ㐪࠸ࡀࣛࢵࢺࡢ⮬
Ⓨ㐠ືࡸᏳ⾜ືཬࡰࡍᙳ㡪ࡘ࠸࡚㸪⬡㉁㐣
㣗ᶆ‽㣗ࢆࡑࢀࡒࢀྠ࣮࢝ࣟࣜ㸭᪥ᢞ᳨࡛ウࢆ
⾜ࡗࡓࠋ
㍯ᅇࡋ⾜ືࡣᴫ᪥ࣜࢬ࣒ࡢ⾜ືࡸ࣓ࢱ࣎ࣜࢵࢡ
ೃ⩌ࡢᨵၿ࡞࡛◊✲ࡉࢀ࡚࠸ࡿࡀ
㻝㻡㻙㻝㻣㻕㸪ᮏ◊✲࡛ࡣ
Ᏻ⾜ືࡢ㛵㐃ᛶࡘ࠸᳨࡚ウࢆ⾜ࡗࡓࠋ㍯ᅇࡋ
⾜ືࡢ⤖ᯝࡽࡳࡓ⮬Ⓨⓗ⾜ື࡛ࡣࡓࢇࡥࡃ㉁ࡸ⬡
㉁ࡢ㉁ࡢ㐪࠸㛵ࢃࡽࡎᙳ㡪ࡣᑡ࡞ࡗࡓࠋⴭ⪅ࡽ
ࡣඛ⾜◊✲࡛Ᏻ⾜ືࢆ࣮࢜ࣜࣈἜ࢚ࢦ࣐Ἔ࡛ẚ
㍑ࡋࡓࡣ୧⪅ࡢἜࡢ㉁ࡢ㐪࠸࡛⾜ືᙳ㡪ࡀࡳ
ࡽࢀࡓ
㻝㻙㻟㸧ࡢẚ࡚㸪⮬Ⓨ⾜ືࡣᏳ⾜ືࡣ␗࡞
ࡿ࣓࢝ࢽࢬ࣒ࡢ㛵ࡀ࠶ࡿ♧၀ࡉࢀࡓࠋ
Ᏻ⾜ື࠾࠸࡚ࡣ㸪ࡓࢇࡥࡃ㉁ࡢ㐪࠸ࡼࡾ⬡㉁
ࡢ㉁ࡢᙳ㡪ࡀࡁ࠸ࡇࡀ᫂ࡽ࡞ࡗࡓࠋ㻴㻲㻰 ࡢ
࣐࢘ࢫ࡛ࡣ㸪Ᏻ⾜ືࡀࢥࣥࢺ࣮࣐ࣟࣝ࢘ࢫࡼࡾప
ࡗࡓࡢሗ࿌
㻝㻠㻕㻌ࡀ࠶ࡿࡀ㸪ᮏ◊✲ࡢࣛࢵࢺ࡛ࡶ࢜
࣮ࣜࣈἜ⩌࡛ࡣ㸪㻴㻲㻰 ࡢ᪉ࡀᶆ‽㣗ࡼࡾ࣮࢜ࣉࣥ㡿 ᇦฟࡿ⾜ືࡀከ࠸ഴྥ࡛࠶ࡗࡓ㸦㻲㼕㼓㻚㻠㸧 ࠋ࢚ࢦ࣐
Ἔ⩌࡛ࡣ㸪㻴㻲㻰 ᶆ‽㣗ࡣࡶ࣮࢜ࣜࣈἜ⩌ࡼ
ࡾ࣮࢜ࣉࣥᮎ➃ࡸ 㻭 㡿ᇦከࡃฟࡿഴྥ࡛࠶ࡗࡓࠋ
࣮࢜ࣉࣥᮎ➃ࡽୗࢆなࡁ㎸ࡴᅇᩘࡀ㢧ⴭ࡞ࡢࡣ࢝
ࢮ࢚ࣥࢦ࣐Ἔࡢ⤌ྜࡏࡢᶆ‽㣗 㻴㻲㻰 ࡛࠶ࡗࡓ 㸦㻲㼕㼓㻚㻡㸧 ࠋ᭦㸪࣮࢜ࣉࣥᮎ➃࡛ࡢࣛࢵࢺࡢ㧨ࡢືࡁ
ࡽ࢚ࢦ࣐Ἔࡢ᪉ࡀ࢝ࢮࣥࡸ㇋ࡶⰋࡃືࡃ ഴྥ࡛࠶ࡗࡓࡢ࡛Ᏻឤࡣᑡ࡞ࡗࡓ⪃࠼ࡽࢀࡿࠋ ᮏ◊✲࡛ࡢᐇ㝿ࡢ࢚ࢦ࣐Ἔᦤྲྀࡢࣛࢵࢺࡢᮎ➃࡛ࡢ
⾜ືࡣᏳࡼࡾࡶ⯆ࢆࡑࡑࡿࡢ㸪࠶ࡿ࠸ࡣ༴㝤
ࡢឤᗘࡀ㕌࠸ࡢ㸪࠸ࡎࢀࡋࢁᏳឤ࠸࠺ࡼ
ࡾࣜࣛࢵࢡࢫࡋ࡚࠸ࡿẼ㓄ࡀࡳࡽࢀࡓࠋ࢚ࢦ࣐Ἔࡀ
࣮࢜ࣜࣈἜࡼࡾᏳឤࡀప࠸ࡢࡣ㸪㨶ἜࡀᏳ
≧ࢆ♧ࡉ࡞ࡗࡓ᪤ሗ
㻝㻤㸧ఝ࡚࠸ࡓࡇࡽᏳឤ
ࡣࡓࢇࡥࡃ㉁ࡼࡾ⬡㉁ࡢ㉁ࡢᙳ㡪ࡀࡁ࠸ࡇ
ࡀ♧၀ࡉࢀࡓࠋ
ࠫȁა
Ᏻ⾜ື
㸯㸬⬡㉁㐣㣗࠾ࡅࡿື≀ᛶࡓࢇࡥࡃ㉁᳜≀ᛶ ࡓࢇࡥࡃ㉁ࡀࣛࢵࢺࡢᏳ⾜ືཬࡰࡍᙳ㡪ࡣ㸪 㣗ྜࢃࡏࡢ⬡㉁ࡢ㉁ࡼࡗ࡚ኚືࡍࡿࡇࡀ᫂
ࡽ࡞ࡗࡓࠋ
㸰㸬⬡㉁㐣㣗ࡀᶆ‽㣗ྠࡌ࣮࢝ࣟࣜࢆᦤྲྀࡋࡓ ሙྜ㸪㼚㻙㻟 ⣔ࡢ࢚ࢦ࣐Ἔࡢ᪉ࡀ 㼚㻙㻥 ⣔ࡢ࣮࢜ࣜࣈἜ
ࡼࡾ࢝ࢮࣥ㇋ࡓࢇࡥࡃ㉁࠾࠸࡚Ᏻ⾜ື
ࡣపࡃࠊⴠࡕ╔࠸࡚࠸ࡓࡢࡀほᐹࡉࢀࡓࠋ
४ࣉࡃ
㻝㸧ᆏᜨᏊ㸪ᮎ⏣ࠉࠉ㸪⬡㉁㐣㣗ࡢ㐣ᦤྲྀ
ࡣࣛࢵࢺࡢᨷᧁ⾜ືࢆᢚไࡋࡓ㸬㮵ඣᓥ⣧ᚰዪᏊ
Ꮫ┳ㆤᰤ㣴Ꮫ㒊⣖せ㸪㻝㻥㸸㻝㻠㻙㻝㻥㸪㻞㻜㻝㻡
㻞㸧ᆏᜨᏊ㸪ஂ㔝▱⨾ࠉ㸪⬡㉁㐣㣗⢾㉁㐣
㣗ࡀࢲ࢚ࢵࢺࡢయ㔜࣭㌟㛗ኚືᨷᧁ࣭Ᏻ⾜
ືཬࡰࡍᙳ㡪㸬㮵ඣᓥ⣧ᚰዪᏊᏛ┳ㆤᰤ㣴Ꮫ 㒊⣖せ㸪㻝㻤㸸㻝㻥㻙㻞㻡㸪㻞㻜㻝㻠
㻟㸧ᆏᜨᏊ㸪୰ᑿ♩ዉࠉ㸪㇋ࡓࢇࡥࡃ㉁࣮࢜ࣜ
ࣈ࢜ࣝ࠾ࡼࡧ࢚ࢦ࣐Ἔࡀࢫࢺࣞࢫ㈇Ⲵࣛࢵࢺࡢ ᨷᧁᛶཬࡰࡍᙳ㡪㸬㮵ඣᓥ⣧ᚰዪᏊᏛ┳ㆤᰤ
࣮࢜ࣉ࣮࣒ࣥࡢᮎ➃࡛ࡢࣛࢵࢺࡢ㧨ࡢືࡁࢆࡳ
ࡿ㸪㇋ࡓࢇࡥࡃ㉁࣮࢜ࣜࣈἜࡢሙྜ㸪㻺 ࡣ㧨ࡢ
ືࡁࡀࡢ⩌ẚ࡚ᑡ࡞ࡗࡓ㸦㻲㼕㼓㻚㻢㸧 ࠋ࢝ࢮࣥ
㇋ࡓࢇࡥࡃ㉁ࡣ࢚ࢦ࣐Ἔࡢ᪉ࡀ 㻴㻲㻰 㻺 ࡶ
࣮࢜ࣜࣈἜࡼࡾ㧨ࡀⰋࡃືࡃഴྥࢆ♧ࡋࡓࠋ
20 25
10 15
0 5
㻭㻦㻌㻻㼘㼕㼢㼑㻌㼛㼕㼘㻌 㻮㻦㻌㻱㼓㼛㼙㼍㻌㼛㼕㼘
20 25
10 15
Whisker score
0 5
Normal HFD Normal HFD Normal HFD Normal HFD
Casein Casein Soy Soy Casein Casein Soy Soy
㻲㼕㼓㻚㻢䚷㼀㼔㼑㻌㼍㼏㼠㼕㼢㼕㼠㼥㻌㼟㼏㼛㼞㼑㼟㻌㼛㼒㻌㼣㼔㼕㼟㼗㼑㼞㼟㻌㼛㼒㻌㼞㼍㼠㼟㻌㼛㼚㻌㼠㼔㼑㻌㼑㼐㼓㼑㻌㼍㼠㻌㼠㼔㼑㻌㼛㼜㼑㼚㻌㼍㼞㼙㻌㼕㼚㻌㻌
㻌㻌㻌㻌㻌㻌㻌㻌㻌㻱㼘㼑㼢㼍㼠㼑㼐㻌㻌㼜㼘㼡㼟㻌㼙㼍㼦㼑㻌㼠㼑㼟㼠㻚㻌㻭㻦㻌㼛㼘㼕㼢㼑㻌㼛㼕㼘㻘㻌㻮㻦㻌㼑㼓㼛㼙㼍㻌㼛㼕㼘㻌㼓㼞㼛㼡㼜㼟㻚
㻔㻟㻜㻕 㮵ඣᓥ⣧ᚰዪᏊᏛ┳ㆤᰤ㣴Ꮫ㒊⣖せ䚷㻌㼂㼛㼘㻚㻞㻜㻘㻌㻞㻜㻝㻢
㣴Ꮫ㒊⣖せ㸪㻝㻢㸸㻣㻙㻝㻞㸪㻞㻜㻝㻞
㻠㸧㼀㼑㼟㼏㼔㼑㼙㼍㼏㼔㼑㼞㻌 㻴㻘㻌 㻻㼜㼕㼛㼕㼐㻌 㼞㼑㼏㼑㼜㼠㼛㼞㻌 㼘㼕㼓㼍㼚㼐㼟㻌 㼐㼑㼞㼕㼢㼑㼐㻌 㼒㼞㼛㼙㻌 㼒㼛㼛㼐㻌 㼜㼞㼛㼠㼑㼕㼚㼟㻚㻌 㻯㼡㼞㼞㻌 㻼㼔㼍㼞㼙㻌 㻰㼑㼟㻚㻌 㻥㸸㻝㻟㻟㻝㻙㻝㻟㻠㻠㸪 㻞㻜㻜㻟
㻡㸧㼅㼛㼟㼔㼕㼗㼍㼣㼍㻌 㻹㸪㼀㼍㼗㼍㼔㼍㼟㼔㼕ࠉ㻹 㸪㼅㼍㼚㼓ࠉ㻿㸪㻰㼑㼘㼠㼍㻌 㼛㼜㼕㼛㼕㼐㻌 㼜㼑㼜㼠㼕㼐㼑㼟㻌 㼐㼑㼞㼕㼢㼑㼐㻌 㼒㼞㼛㼙㻌 㼜㼘㼍㼚㼠㻌 㼜㼞㼛㼠㼑㼕㼚㼟㻚㻌 㻯㼡㼞㼞㻌 㻼㼔㼍㼞㼙㻌㻰㼑㼟㻘㻌㻥㸸㻝㻟㻞㻡㻙㻝㻟㻟㻜㸪㻞㻜㻜㻟
㻢㸧㻸㼕㼟㼠㼑㼞㻌 㻶㻘㻌 㻲㼘㼑㼠㼏㼔㼑㼞㻌 㻼㻶㻘㻌 㼑㼠㻌 㼍㼘㸸㻮㼑㼔㼍㼢㼕㼛㼞㼍㼘㻌 㼑㼒㼒㼑㼏㼠㼟㻌 㼛㼒㻌 㼒㼛㼛㼐㻙㼐㼑㼞㼕㼢㼑㼐㻌 㼛㼜㼕㼛㼕㼐㻙㼘㼕㼗㼑㻌 㼜㼑㼜㼠㼕㼐㼑㼟㻌 㼕㼚㻌 㼞㼛㼐㼑㼚㼠㼟㻚㻌 㻼㼔㼍㼞㼙㼍㼏㼛㼘㻌㻮㼕㼛㼏㼔㼑㼙㻌㻮㼑㼔㼍㼢㻚㻘㻌㻝㻟㻠㸸㻣㻜㻙㻣㻤㸪㻞㻜㻝㻡 㻣㸧㼀㼞㼕㼢㼑㼐㼕㻌 㻹㻿㻘㻌 㻿㼔㼍㼔㻌 㻶㻿㻘㻌 㼑㼠㻌 㼍㼘㸸㻲㼛㼛㼐㻙㼐㼑㼞㼕㼢㼑㼐㻌 㼛㼜㼕㼛㼕㼐㻌
㼜㼑㼜㼠㼕㼐㼑㼟㻌 㼕㼚㼔㼕㼎㼕㼠㻌 㼏㼥㼟㼠㼑㼕㼚㼑㻌 㼡㼜㼠㼍㼗㼑㻌 㼣㼕㼠㼔㻌 㼞㼑㼐㼛㼤㻌 㼍㼚㼐㻌 㼑㼜㼕㼓㼑㼚㼑㼠㼕㼏㻌 㼏㼛㼚㼟㼑㼝㼡㼑㼚㼏㼑㼟㻚㻌 㻶㻌 㻺㼡㼠㼞㻌 㻮㼕㼛㼏㼔㼑㼙㻘㻌 㻞㻡㸸㻝㻜㻝㻝㻙 㻝㻜㻝㻤㸪㻞㻜㻝㻠
㻤㸧㻿㼕㼙㼛㼚㻌 㻺㻳㻘㻌 㻷㼍㼜㼘㼍㼚㻌 㻶㻾㻘㻌 㼑㼠㻌 㼍㼘㸸㻵㼚㼏㼞㼑㼍㼟㼑㼐㻌 㼍㼓㼓㼞㼑㼟㼟㼕㼢㼑㻌 㼎㼑㼔㼍㼢㼕㼛㼞㻌 㼍㼚㼐㻌 㼐㼑㼏㼞㼑㼍㼟㼑㼐㻌 㼍㼒㼒㼕㼘㼕㼍㼠㼕㼢㼑㻌 㼎㼑㼔㼍㼢㼕㼛㼞㻌 㼕㼚㻌 㼍㼐㼡㼘㼠㻌 㼙㼍㼘㼑㻌 㼙㼛㼚㼗㼑㼥㼟㻌 㼍㼒㼠㼑㼞㻌 㼘㼛㼚㼓㻙㼠㼑㼞㼙㻌 㼏㼛㼚㼟㼡㼙㼜㼠㼕㼛㼚㻌 㼛㼒㻌 㼐㼕㼑㼠㼟㻌 㼞㼕㼏㼔㻌㼕㼚㻌㼟㼛㼥㻌㼜㼞㼛㼠㼑㼕㼚㻌㼍㼚㼐㻌㼕㼟㼛䟻㼍㼢㼛㼚㼑㼟㻚㻌㻴㼛㼞㼙㻌㻮㼑㼔㼍㼢㻘㻌㻠㻡㸸 㻞㻣㻤㻙㻞㻤㻠㸪㻞㻜㻜㻠
㻥㸧㻸㼑㼜㼔㼍㼞㼠㻌 㻱㻰㻘㻌 㼃㼑㼟㼠㻌 㼀㼃㻘㻌 㼑㼠㻌 㼍㼘㸸㻺㼑㼡㼞㼛㼎㼑㼔㼍㼢㼕㼛㼞㼍㼘㻌 㼑㼒㼒㼑㼏㼠㼟㻌 㼛㼒㻌 㼐㼕㼑㼠㼍㼞㼥㻌 㼟㼛㼥㻌 㼜㼔㼥㼠㼛㼑㼟㼠㼞㼛㼓㼑㼚㼟㻚㻌 㻺㼑㼡㼞㼛㼠㼛㼤㼕㼏㼛㼘㻌 㼀㼑㼞㼍㼠㼛㼘㻘㻌㻞㻠㸸㻡㻙㻝㻢㸪㻞㻜㻜㻞
㻝㻜㸧㻴㼑㼍㼠㼔㼑㼞㻌㻶㻌㻸㼑㼕㼐㼥㻘㻌㻯㼔㼑㼘㼟㼕㼑㻌㻮㻌㼀㼛㼐㼐㻘㻌㼑㼠㻌㼍㼘㸸㻯㼛㼚㼟㼡㼙㼕㼚㼓㻌 㼔㼕㼓㼔㻙㼜㼞㼛㼠㼑㼕㼚㻌 㼟㼛㼥㻌 㼟㼚㼍㼏㼗㼟㻌 㼍㼒㼒㼑㼏㼠㼟㻌 㼍㼜㼜㼑㼠㼕㼠㼑㻌 㼏㼛㼚㼠㼞㼛㼘㻘㻌 㼟㼍㼠㼕㼑㼠㼥㻘㻌㼍㼚㼐㻌㼐㼕㼑㼠㻌㼝㼡㼍㼘㼕㼠㼥㻌㼕㼚㻌㼥㼛㼡㼚㼓㻌㼜㼑㼛㼜㼘㼑㻌㼍㼚㼐㻌㼕㼚䟻㼡㼑㼚㼏㼑㼟㻌 㼟㼑㼘㼑㼏㼠㻌 㼍㼟㼜㼑㼏㼠㼟㻌 㼛㼒㻌 㼙㼛㼛㼐㻌 㼍㼚㼐㻌 㼏㼛㼓㼚㼕㼠㼕㼛㼚㻚㻌 㻶㻌 㻺㼡㼠㼞㻘㻌 㻝㻠㻡㸸 㻝㻢㻝㻠㻙㻝㻢㻞㻞㸪㻞㻜㻝㻡
㻝㻝㸧㻭㼚㼐㼞㼑㻌 㻯㻘㻌 㻰㼕㼚㼑㼘㻌 㻭㻸㻘㻌 㼑㼠㻌 㼍㼘㸸㻰㼕㼑㼠㻙㼕㼚㼐㼡㼏㼑㼐㻌 㼛㼎㼑㼟㼕㼠㼥㻌
㼜㼞㼛㼓㼞㼑㼟㼟㼕㼢㼑㼘㼥㻌 㼍㼘㼠㼑㼞㼟㻌 㼏㼛㼓㼚㼕㼠㼕㼛㼚㻘㻌 㼍㼚㼤㼕㼑㼠㼥㻙㼘㼕㼗㼑㻌 㼎㼑㼔㼍㼢㼕㼛㼞㻌 㼍㼚㼐㻌 㼘㼕㼜㼛㼜㼛㼘㼥㼟㼍㼏㼏㼔㼍㼞㼕㼐㼑㻙㼕㼚㼐㼡㼏㼑㼐㻌 㼐㼑㼜㼞㼑㼟㼟㼕㼢㼑㻙㼘㼕㼗㼑㻌 㼎㼑㼔㼍㼢㼕㼛㼞㻚㻌㻮㼞㼍㼕㼚㻌㻮㼑㼔㼍㼢㻌㻵㼙㼙㼡㼚㻘㻌㻠㻝㸸㻝㻜㻙㻞㻝㸪㻞㻜㻝㻠 㻝㻞㸧㻻㼔㼘㼍㼚㼐㻌㻯㻸㻘㻌㻼㼍㼚㼗㼕㼢㻌㻱㻘㻌㼑㼠㻌㼍㼘㸸㼃㼑㼟㼠㼑㼞㼚㻌㼐㼕㼑㼠㻙㼕㼚㼐㼡㼏㼑㼐㻌
㼍㼚㼤㼕㼛㼘㼥㼠㼕㼏㻌㼑㼒㼒㼑㼏㼠㼟㻌㼕㼚㻌㼙㼕㼏㼑㻌㼍㼞㼑㻌㼍㼟㼟㼛㼏㼕㼍㼠㼑㼐㻌㼣㼕㼠㼔㻌㼍㼘㼠㼑㼞㼍㼠㼕㼛㼚㼟㻌 㼕㼚㻌㼠㼞㼥㼜㼠㼛㼜㼔㼍㼚㻌㼙㼑㼠㼍㼎㼛㼘㼕㼟㼙㻚㻌㻺㼡㼠㼞㻌㻺㼑㼡㼞㼛㼟㼏㼕㻘㻌㻶㼡㼚㻌㻝㻤㻚㻱㼜㼡㼎㻚㻌 㻞㻜㻝㻡
㻝㻟㸧㻹㼡㼓㼚㼡㼟㼟㼛㼚㻌 㻷㻾㻘㻌 㻴㼍㼡㼏㼗㻌 㻸㻘㻌 㼑㼠㻌 㼍㼘㸸㻾㼑㼘㼍㼠㼕㼛㼚㼟㼔㼕㼜㼟㻌 㼎㼑㼠㼣㼑㼑㼚㻌 㼐㼕㼑㼠㻙㼞㼑㼘㼍㼠㼑㼐㻌 㼏㼔㼍㼚㼓㼑㼟㻌 㼕㼚㻌 㼠㼔㼑㻌 㼓㼡㼠㻌 㼙㼕㼏㼞㼛㼎㼕㼛㼙㼑㻌 㼍㼚㼐㻌㼏㼛㼓㼚㼕㼠㼕㼢㼑㻌䟻㼑㼤㼕㼎㼕㼘㼕㼠㼥㻚㻌㻺㼑㼡㼞㼛㼟㼏㼕㼑㼚㼏㼑㻘㻌㻟㻜㻜㸸㻝㻞㻤㻙㻝㻠㻜㸪 㻞㻜㻝㻡
㻝㻠㸧㻼㼥㼚㼐㼠㻌 㻶㼛㼞㼓㼑㼚㼟㼑㼚㻌 㻮㻘㻌 㻴㼍㼚㼟㼑㼚㻌 㻶㼀㻘㸸㻭㻌 㼜㼛㼟㼟㼕㼎㼘㼑㻌 㼘㼕㼚㼗㻌 㼎㼑㼠㼣㼑㼑㼚㻌㼒㼛㼛㼐㻌㼍㼚㼐㻌㼙㼛㼛㼐㻚㻌㻼㻸㼛㼟㻌㻻㼚㼑㻚㻌㻥㸸㼑㻝㻜㻟㻟㻥㻤㻘㻌㻞㻜㻝㻠㻌 㻝㻡㸧㻰㼑㻌㻹㼍㼠㼠㼑㼕㼟㻌㻾㻘㻌㻸㼡㼏㼑㼞㼠㼕㼚㼕㻌㻲㻘㻌㼑㼠㻌㼍㼘㻚㻘㻌㻱㼤㼑㼞㼏㼕㼟㼑㻌㼍㼟㻌㼍㻌㼚㼑㼣㻌 㼜㼔㼥㼟㼕㼛㼘㼛㼓㼕㼏㼍㼘㻌㼟㼠㼕㼙㼡㼘㼡㼟㻌㼒㼛㼞㻌㼎㼞㼛㼣㼚㻌㼍㼐㼕㼜㼛㼟㼑㻌㼠㼕㼟㼟㼡㼑㻌㼍㼏㼠㼕㼢㼕㼠㼥㻚㻌 㻺㼡㼠㼞㻌㻹㼑㼠㼍㼎㻌㻯㼍㼞㼐㼕㼛㼢㼍㼟㼏㻌㻰㼕㼟㻘㻌㻞㻟㻦㻡㻤㻞㻙㻡㻥㻜㻘㻌㻞㻜㻝㻟
㻝㻢㸧㼂㼛㼖㼠㻌 㼏㼔㻌 㻿㼗㼛㼜㻘㻌 㻴㼍㼚㼍㻌 㻹㼍㼘㼕㼚㼟㼗㼍㻘㻌 㼑㼠㻌 㼍㼘㻚㻘㻌 㻼㼛㼟㼕㼠㼕㼢㼑㻌 㼑㼒㼒㼑㼏㼠㼟㻌 㼛㼒㻌 㼢㼛㼘㼡㼚㼠㼍㼞㼥㻌 㼞㼡㼚㼚㼕㼚㼓㻌 㼛㼚㻌 㼙㼑㼠㼍㼎㼛㼘㼕㼏㻌 㼟㼥㼚㼐㼞㼛㼙㼑㻙 㼞 㼑 㼘 㼍 㼠 㼑 㼐 㻌 㼐 㼕 㼟 㼛 㼞 㼐 㼑 㼞 㼟 㻌 㼕 㼚 㻌 㼚 㼛 㼚 㻙 㼛 㼎 㼑 㼟 㼑 㻌 㼔 㼑 㼞 㼑 㼐 㼕 㼠 㼍 㼞 㼥㻌 㼔㼥㼜㼑㼞㼠㼞㼕㼍㼏㼥㼘㼓㼘㼥㼏㼑㼞㼛㼘㼑㼙㼕㼏㻌㼞㼍㼠㼟㻚㻌㻼㼘㼛㼟㻌㻻㼚㼑㻘㻌㻝㻜㻦㼑㻜㻝㻞㻞㻣㻢㻤㻘㻌 㻞㻜㻝㻡
㻝㻣㸧ྜྷ⏣㸪㼀㼟㼡㼗㼡㼎㼍 ື⣔ࣛࢵࢺࡢᴫ᪥ࣜࢬ࣒ࡢ
≉ᚩ㸸㍯ᅇࡋ⾜ືࡼࡿ᳨ウ㸬ோឡᏛ◊✲⣖せ 㻘㻌㻤㻦㻌 㻡㻟㻙㻡㻥㻘㻞㻜㻜㻥
㻝㻤㸧㻷㼡㼔㼚㻌 㻲㼀㻘㻌 㻾㼛㼢㼑㼞㼟㼕㻌 㻷㻘㻌 㼑㼠㻌 㼍㼘㻌 㸸㻵㼚㼒㼘㼡㼑㼚㼏㼑㻌 㼛㼒㻌 㼠㼞㼍㼚㼟㻌 㼒㼍㼠㻌
㼍㼚㼐㻌 㼛㼙㼑㼓㼍㻙㻟㻌 㼛㼚㻌 㼠㼔㼑㻌 㼜㼞㼑㼒㼑㼞㼑㼚㼏㼑㻌 㼛㼒㻌 㼜㼟㼥㼏㼔㼛㼟㼠㼕㼙㼡㼘㼍㼚㼠㻌
㼐㼞㼡㼓㼟㻌㼕㼚㻌㼠㼔㼑㻌䟺㼞㼟㼠㻌㼓㼑㼚㼑㼞㼍㼠㼕㼛㼚㻌㼛㼒㻌㼥㼛㼡㼚㼓㻌㼞㼍㼠㼟㻚㻌㻼㼔㼍㼞㼙㼍㼏㼛㼘㻌
㻮㼕㼛㼏㼔㼑㼙㻌㻮㼑㼔㼍㼢㻚㻌㻝㻝㻜㸸㻡㻤㻙㻢㻡㸪㻞㻜㻝㻟
㻔㻟㻝㻕 㼀㼔㼑㻌㼏㼛㼙㼜㼍㼞㼕㼟㼛㼚㻌㼛㼒㻌㼍㼚㼕㼙㼍㼘㻌㼜㼞㼛㼠㼑㼕㼚㻌㼍㼚㼐㻌㼜㼘㼍㼚㼠㻌㼜㼞㼛㼠㼑㼕㼚㻌㼛㼚㻌㼟㼜㼛㼚㼠㼍㼚㼑㼛㼡㼟㻌㼣㼔㼑㼑㼘㻌㼞㼡㼚㼚㼕㼚㼓㻌㼍㼚㼐㻌㼍㼚㼤㼕㼛㼡㼟㻌㼎㼑㼔㼍㼢㼕㼛㼞㻌㼛㼒㻌㼞㼍㼠㼟㻌㼒㼑㼐㻌㼔㼕㼓㼔㻌㼒㼍㼠㻌㼐㼕㼑㼠㻚
The comparison of animal protein and plant protein on spontaneous wheel running and anxious behavior of rats fed high fat diet.
Keiko Sakai, Kotomi Fukushige
Department of Health and Nutrition, Faculty of Nursing and Nutrition, Kagoshima Immaculate Heart University
Key words：high fat diet, spontaneous wheel running, anxious behavior, soy protein/soybean protein, fatty acid
Abstract
Purpose: the aim of this study was to investigate the difference between casein and soy protein with either olive oil (n-9 series fatty acid) or egoma oil (n-3 series fatty acid) on spontaneous exercise and anxious behavior of rats fed high fat diet.
Method: Sprague-Dawley rats fed either casein or soy protein with olive oil or egoma oil for twelve weeks.
Further, each diet group had both high fat diet (HFD) and Normal diet. Behavior experiment was conducted by Spontaneous Wheel Running and Elevated Plus Maze test. Animal experiment was approved by institutional committee.
Result: In this study, rats of each diet group was fed same amount of calories. The spontaneous activity was higher in HFD of egoma oil with casein and soy protein than Normal diet, especially soy protein with egoma oil. Anxious behavior of HFD was inÁuenced by quality of fatty acids rather than quality of protein. Egoma oil with casein and soy protein showed longer staying time at edge of open area on elevated plus maze than olive oil. However, the high activity of whiskers of egoma oil at the edge area seems to be curiosity rather than anxiety.
Conclusion: Spontaneous exercise and anxious behavior were influenced by both protein and fatty acid.
Anxious behavior was depend on quality of fatty acid rather than protein.
