Regulatory Roles of Extracellular Matrix Metalloproteinase Inducer in the Bovine Endometrial Function during Gestation

(1)

Title

Regulatory Roles of Extracellular Matrix Metalloproteinase

Inducer in the Bovine Endometrial Function during Gestation( 内

容の要旨(Summary) )

Author(s)

MISHRA, Birendra

Report No.(Doctoral

Degree)

博士(獣医学) 甲第320号

Issue Date

2011-03-14

Type

博士論文

Version

none

URL

http://hdl.handle.net/20.500.12099/36605

※この資料の著作権は、各資料の著者・学協会・出版社等に帰属します。

(2)

fEB (i (Eg) f5) j= fE _* _* A _3B S Jk ∼ * * JBS Ik %E * i JB3?Iki2 _{J5. *} ,q B JB3?I%i Ji. a) i 1* WRS+&tFjS* #gEfB* %5% 81f=*JB3? &it lk i& i Rg P % jE i A

MISIRA, Birendra _{(%,/i-}t,3*fBf%j=jf*DB)}

jESj(JB3? #]2 1# JTt - 3E; F= t*i (5EB;) #B;i*EP#3 2 _0% 5FBE23*3Jq14B '9+IkRRu# 3 _A# 1 _q!#j 3*%# BE*#gES+ 5E B;_JB3?*4c jE S jc JB3?

Regulatory Roles of Extracellular Matrix

Metalloproteinase Inducer in the Bovine Endon)etrial

Function during Gestation

(=>1 U ya) _{9 ->#hE+7gFqBS*BE&1#t=Bg+8m#E)} j=i jE S j( _JB3? 6uZ& #LESiE*JB3? 6ui i S jc $ 6ui _3E#B%I*J$3? 6ui _{d& ji} jc _JBS ･# a ･# & # # # & # 32 EiE# ai FE %A i 5: _J% 4# JR - ig; I iln75# 3S je i A i ie ;a 3t 0) _Fq * Q) S B

+gPqBSo),%R#&U %fhu >g>)bS5%BeB= _{i J3TifThb8} I _{i 8i= 5 i:-Chbt2Ul.} _5EBeBS

eB&4gn3Bt, _{JEDikhF$8=h-U,-C+7gPqB#8=Ej:4&#Ft:B9,21t:bS}i f= 6 gn, 3: f=i#iiEBb8= b@lB9 ti'21EbS& t= 8. *fhE8=L# 5 +iFfqBSnF'j + _{-I.y 9} tj:#R51%u %jlh) U >9x13:#F]B&9[? h u

y 9 A (ECM) _{0)/21E8=&-j%,} ECM _{L^ij5Sjs,it):i5f[V5#0)#*j;t?/<7>RbS+gPqB#e)1#}

Retb9'21Eh13:tFS#if4&BE3Egat@.4fC=F*lRf LTU,a i %k 6nao =0) i 5 7tf ECM 0)1i;

atmfFEi7 f, U y?R} F t=7pDf7-ifh (MMP) e%n?xqiifPg+L=i:-3TSIJ4#5n, 5

68=+%F5BS ECM 8=8j:%qO)+j I,A/ _{>%te#Eq+#75S,a?#5hTU,a} I ti),6, ECM

ek'2F$0) =h E,Eq+Q)iKP#tj:+gPqBS#.PB&0) MMP _{#fjab%bf=i%#6q,21bk} b f= 6+ i 5%k6h8o

ExtraceIlular _matrix _{metaIloproteinase} inducer (EMMPRIN) Ej:j5?i9h-p 7- ₎ >X-/i-7 7 -I 9 C=BS+8#F]B&B##i5F >/<9 #7?Ab 9, #Jk a),WB&8=3blU'T MMP nFA4=kfe2hpS%a

I _tbS*p _6h-CU,8. _Lb,Lt?bS _6, _{9->jlgPqB#8=b'818} _EMMPRIN _{0)#%h'J=t>'42%I]}

C=-3U'Tlj:82 2=_{AJt'1*-i45hTU't2U1.}

*Wql.T13:, 9 _{t>+%PqBSt=b-818} EMMPRIN _{0)4&#ibb4k%1jkPB bi),8=+a} I i & E]b9 i L,, nJfO) 3 _{I/q8=-)V,T1*EELf=o}

(3)

1. _A,r#Ja3g]jbl

cttF5EBe5Fiii+7EPqESC=jb'Ll a EMMPRIN a)42Su

A-*c=ib'U ,TGj:, A.r#Ja,q3b'i:Ui>5EB;5?f6+7EPqBSC=h181 a EMMPRIN 0)#ga#ShEl ja7Ei:7.y!)]-<ai,ifC=, EMMPRIN 8%iEo)fB+,4!t L,T MMP # (MMP-1, _-2, _-7, _-9, _-13

is-cttFL14) i ADAMTS-1 0)3E%8=-?U,T;a7.7<, +fPqD,qC=h'Cla EMMPRIN a)&JL%Tjt=-?ul T5%* LT=o EMMPRIN _{8i9,J+EPS)Jg8=3e%} L, #t#Jaf9]i=TET,C,T%0)&SLlqb';#iKlb L,,

3gJr#qj,q8=F%U,#fRk,it Lf=o _{55Be,qPqBStjk#tuD&-ehO)} EMMPRIN 3e%Ej:1&T L,, MMP

#jb'itJ: ADAMTS-1 _{0)#%b5GtiLL.i} _-a Lf=o _-i, %B:E5S5>3gO)Su&F3i4h8=jb'UlrC

EMMPRIN _{0)5&V ,5EfRk=#hbf=o} _=h _6o)#Ab, 6 , 5EBeFS#n+gPqE*EMMPRJN Ei MMP

#jt;i: Ut' ADAMTS-1 _0)F3i4=S[J4#ki5 LT+gPqB#0)Eb,2%j=*+8 I i bS,j%P&5 hf=o 3: f=, _{55Be5?i50)#i(iAS-Chn5&V} _,#%b, ₆ , %BeF$0)#FeB##PB& i +7EPqB#tjk,*PB&0)4#5E,

&%8=BgJi+8 t*k 6YLf=.

2. _{*fW{+EEihPq)]*8=3b'Ll}a EMMPRJN 0)42%[l #=* 8=3b' U _,TEj:, *ih&3g]o)+7EPqBSh.

ettJi>#1iiLinSt=h'L18 EMMPRIN 0)4&iEB912%u k +gPq)]R B&SJgB$4=5e%-fa MMP #, ADAMTS-1 h'iUVj<o f.-->RF*li*Eq+0)#fiLEtk

i5 L _T*Ejf L 7T=o EMMPRIN _{0)#%8ij=8=+i/NiO)tjk#P]B&jb'} i:_{U<=#,*F]B&k%b##} B*#F]B&8=ia¥hbE,a, MMP-14 i ADAMTS-1 _{0)#%bF]4#ifJaqj=k,j%} L,FIBS, MMP-2 0)3E giG3:+i,JlftO)Fpqgkgh&8=5E%&-iBhbf=. 5 6 8=, EMMPRIN _{0)#%Ei*ftuEi&,qO)B&4ggF}

veh#%8=teun L,, MMP-14 3a-i:t>+ADAMTS-1 b W#tf3e%&%tL.T>bJ3 f=. _=h60)J#Abl 6 , EMMPRIN bSiftuE4&no)B&agB58=ib,V,T MMP-14 3b-i Ui+ ADAMTS-1 a)BE4=k1&L#, J{Di

&niW.8=F*lJ5-+a I i

_ASq:lit:.

*fhE1&m7:hO) BAX, Fas, FasL j3,iUth TNFcLif i.7*o h -.JR1&L#Ba+a)#ZRbS*fhE@;gO)B&.SRgF8=]a,U,-C#%8=&15k* Lf=. =31 6n#Atj:,

EMMPRIN ti MMP #, ADAMTS-1 _jb'iUV*o _{f,-->X1&L#Eq+i:} FxlL*L,I, *fARO),ftBfS

jb'_{itF>Lji&i*&8=isU,T} _{b %ft3:12%(i} _k3Rf=+I _i _&,ii@i-8o

3. _+%PW# _MMP

0)3E%*TJ4*L=3b.B18 EMMPRIN _{0)8R#E:bb4k%r)0)Pi7'fiF}

#=*81h'u _,TEA EMMPR]N _0)#%$lJ4#3S i tF EMMPRIN t= i 8 MMP _# i ADAMTS-1

#Ro)Su4#8%i#C=-)U,Tag/<a FIB, _{9.>+%PqB*B*tBt#,mB&ib'} i _{tFFRq#,*PB&kj]]}ulT=

in vitro rehO)A5$8fk#*f=. i:%iXi+%PqBypq##.mB&C=7pT= -}I Xj= u _y%kk]5! Lf= i I a,

EMMPRIN _{n#fRE3:i%8=t*hR} _Lf=bS, _IR _h

t=.}I yE3:%B?.#i-!i:&82h5tfb,-?f=. -jf,

MMP-2 h'it>.-14 _{0)3E%Ej:7pT].}IXjlt=} _>h'iUIX h t=

-}I>0)RTL358=i D '21k&ip.i a+fb,J3f=o ADAMTS-1 _{0)3EfRij:7t=,}IXjl} t= _JklTL%8=i _D _fCmnLT=o EMMPR]N ASi5 3Ega+8 +%PqDLEtjk*P]B& i 82 i A, ttt#ga L,+i U_{,Fpq5E*.JuB&0)jt-Lg4:$9S*Thij:,}

jit-f%iXi6=i D _{Fpqff,*F]B&Tke)}MMP-2 A, iV'-14 _0)3Egab;fun Lf=. i,;<laAEiEMMPRIN btt4=8=i n'b'9e Lf== i i),6, tjk,1:mB&TChS3E%18 EMMPRIN bSFRqflk:mB&Jt-d13fb98=1fB]LT MMP # 0)3EfRktuDS%a t#t&]J 5hf=. 5 68= EMMPRIN LAL+q,a)5:mB&gH<1'} _JI y I _0)%BkJ<

7p91 flk}Tt-f%.1XiZ;i=t#hpLf= i I a, MMP-14 _{a)3Egateu&Z)1;nSu} ghT== _{i 75,6, tjk#tP} B&&# EMMPRIN Gj:7pT= _-}= A i t= _{>bSfSluSk,j%+3R#1#f8=h,V,I,} _{%n5ii+*o),*F]B&9L}

f:}

JJ> I k* LTFpqf%,1:mD5JO)MMP #0)#%k.'l;-!J4#L,TU,a t##rJ 53tf=.

i;A_Eo)i: 5 8=*mgET13:, 1_{) 9.J+gPqJILEt&k:mD&t=} EMMPRIN bS3Efji+8 I i, 2 ) EMMPRIN Ej:7pT= _-}I Xjl t= _{yJ&G=fbb{i*1#bW3:#%d7lMLE.k'j%+I} i

, 3 ) EMMPRIN 8j:,

ECM _L^YVF5*-A:-_{PxqiiE_Eq}₊ ₀₎ _MMP _e _ADAMTS-1 tf _i.1_{0)3efRSTJ1#kn-} L T _{ll.BeJPif[niO)Bkit} ,

(4)

/ > I _E=i&rf-fa I _{has,j%PA*ghf=o} _=h6nCAEL EMMPRIN k*,i,i Lf=, 5%Dbe*I hE,1tC3$8=isti+_a+7gPqDbM fjlt. U >9>>WgBO)#Rmk*..< b.a)-Chb 9 , 9 ->7e=11Thti < fb

0)m4b i i b T ,

-?-nn65Eth&%L^iubiSkjfti2ftO)PIJ"F.ASfmR3EJ,tO)#b]RJi:iBBf+8o

* 2E a 3R 0) S a

gA %a Eh /2EEq + 0)

-fG 7?.A 8 Extracellular _matrix _{metalloproteinase} inducer

(EMMPRIN) _{0)9,>+7EPW*t=jbt81a&$lJkPA} _6A,8=+8= i kPB9t L, njf

0) 3 I/qk4*;a L, EMMPruN ∼ 9 _{,>+7EPqnST.0)#RD8j%%b[:kPB} 6b,8= Lf=.

1. #Jr#.Jq3gh' i Ulh%BR5?i5+%PqBEiE8=3b, 81a EMMPRIN _0)1%%[j

#-i?3t7?1j:, #Jr-5-Jq3g3si Ui>S-BR3gj17g PqBkfC=js G18 EMMPRJN n#fR#:R-uE. k*

fa Lf=. +gPqB*ThO) EMMPNN _{0)#fREi#.r6iqj3g8=S} _< , %BR#TChEj:+i_Eji#.m

B&vC>#fR75S1&f, ##BSk:M&-C.S#% L,, MMP *3s

cEUi. ADAMTS-1 n#fRtj:

EMMPRIN _n5EfRt-&Lf=. _{=ilbe),##c75,6,} _%BeF$3mo) EMMPRIN tj:MMP

#h'iVh ADAMTS-1 _iE4:ki5 _{LT+%PqB$0)ek,2kj=*} L, #*BSk:PB& i +EPS

BStBE#tmB&0)4*5E, A*%8=rqji+a i Twi5fWAL,flo

2. *IhR+7EPqB#8=b181 8 EMMPRIN _e)&%rJ

#=*TIL *IhR3g+7gPqB*8=h'618 EMMPRIN _4#iEk EMMPRIN, MMP *,

ADJMTS-1 _jbli _U!7j{ _I,_-.>R _{BgLfEq+0)#kBki8} _L,T*ai _Lf=o _EMMPRIN _Ej:+

7EPqBSt&#mB&3a' i _{tF=1##PB&8==#hb,} MMP-2,

-14 i ADAMTS-1 i R#t2ja7Ek

･j%Lf=. 3: f=, EMMPRIN Ej:*fhE%#nB&&fB-chiBhD L, MMP-14 A,i:tF'ADAMTS-1

b W4S+2#fR7fhb-' f=. hnkT, _1NFcL% FasL t2 _i.a)7*o _{I,->xF*qi*W+D*5iE}

A,E, EMMPfuN, MMP #, ADAMTS-1 A,itF7*io T,-->REq+tj:, ifh&o),##h,

i _{t>.L^iihi*&8=3Sft?&SrJkBu=+} _i _#i& _Lf=o

3. _j1%PqBS MMP _{a)#fRSu4#8=ja,} _L1₈ _EMMPRIN _{n1#%EBb4kJLSTJe)*W}

#=*T13:, _{9 ->+gPqBSB]} _#tjk#,ph&3b, _i _{U(FRqE#PB&%jq} v_,f= _{in vitro} ASiFT# 8=

a: 9 _{%n8jiSEASiFi:#*f=o} _7pT] _,}I A jlT= _{yt3:+gPgB#Fpq#,*mB&T.n} EMMPNN

0)#fRk1&L# Lf=bS, =R _{h t].}= >Ei,#*i*k2iE2h5} _tfb,-3f=. _{+7EFqB#t&#mB&}

i _{Fp5#,*mB&o)jf*#jSd5&Tlj:,} _F,q##mB&Tehn _MMP-2 _{3a,i U(14} _#%bStun _Lf=. _i

5iiaAGiEMMPNN _bti*8= _{i 9} _{1t#9e, 3:}_f= _EMMPRIN _n#M&9H{t.} _/ y I _D%BkJ<7p 51 rITEL MMP-14 _{0)#fjifB5&k*$lJLf=o} _=YL6n= _{i 75,6,} _tjk#MS]eiEb# EMMPRJN _tj:#tPB&gHI}

Jly I i:AtLTF,qE#B]h&n MMP #n#fRkSfJ4#+8 i a #L,flo

*WgEEj:, EMMPRIN _{Ej:5tB&e*fhRk##8=j3L1} _8+gPqBS _U _%fth _u _vo)j=*fBa

TChb8= i &#bbTP^6b,8= Lf=.

nJt8=-j U 1_T

, *i45A4iA-%7:h*5a3cbSu*jijcJB3?j<JB3?R3ELf%igAEEJgSW#jC*4o)

(5)

aEi5 i tf _a _J*#i-6[nAI

1) AiB E]

% a _f%

JB3?81ffi# --i7Ef% *.%.AT

Expression _{of extracellular} _matrix _{metalloproteinase} inducer _(EMMPRIN) and its related extracellular matrix degrading enzymes in the endometrium during estrous cycle and early gestation in cattle

Mishra, B., Kizaki, K., Koshi, K., Ushizawa, K., Takahashi, T., Hosoe, M., Sato, T.,It6,_{A. and Hashizume,} K.

Reproductive Biology _and Endocrinology