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Effect of Daylength on the Flower Bud Differentiation and Development in Coriander (Coriandrum sativum L.)

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E#ect of Daylength on the Flower Bud

Di#erentiation and Development in

Coriander (Coriandrum sativum L.)

By

Yaichibe T

OMITAKA

*, Atsukiyo K

ARIMATA

* and Arisa N

OGUCHI

**

(Received May -*, ,**+/Accepted September ,*, ,**+)

Summary : This study examined the influence of daylength on the flower bud di#erentiation and development of coriander. Daylength of 2, +,, +., +0-h and natural daylength were a#orded the plants. The plant height of longer daylength was greater, while daylength had little influence on the number of leaves. The number of days from sowing to flower bud di#erentiation was the smallest for the +0-h daylength (.3 days) and the greatest for the 2-h daylength (1- days). On the other hand, the longer the daylength, the smaller the number of days from flower bud di#erentiation to flowering. The number of expanded leaves was smaller for longer daylength. Flower bud di#erentiation was observed even in the 2-h daylength, although it was earlier in the longer daylength. Therefore, coriander can be regarded as a quantitative long day plant as regards the flower bud di#erentiation. The stage of flower bud development can be classified into the following nine stages :

+) vegetative stage ; ,) predi#erentiation stage ; -) cluster di#erentiation stage ; .) lateral cluster di#erentiation stage ; /) early stage of floret formation ; 0) later stage of floret formation ; 1) petal development stage ; 2) stamen development stage ; 3) carpel development stage.

Key Words : Coriander, Daylength, Development, Flower bud di#erentiation, Quantitative long day plant

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Introduction

Coriander is an umbelliferous annual plant originat-ing in the eastern Mediterranean region. The fruits and young leaves of the plant are commonly used as a condiment herb for their flavor. The fruits are used as an ingredient to prepare curry dishes, or ground to put in various meat dishes and cakes. The leaves are used in a variety of dishes such as soup and meat+῎

. In recent years, an increasing number of coriander plants have been grown and supplied to the market. They have become so popular that production is in demand through the year. Since coriander is both heat and cold tolerant, it can be grown through the year. As prema-ture bolting has often been a problem in summer plant-ing, it is very important to clarify the physiology of flowering.

With regard to the flower bud di#erentiation on umbellifers, it has been found that the flower bud

di#erentiation of carrots and celeries is hastened by low temperature, and that of dills and fennels is stimulated by long day. However, little research has been done on the flower bud di#erentiation on corian-der. The objective of this study was to determine the e#ect of daylength on the flower bud di#erentiation and development in coriander.

Materials and Methods

Seeds were sown on ,0 September +33-, in plastic flats. Plants were transplanted at three true leaf stage in +/ cm plastic pots containing a mixture of equal portions of soil and bark manure.

The plants were given daylength of 2, +,, +., +0 hours and natural daylength. The 2-h treatment plants were exposed to sunlight from 2 : ** am to . : ** pm and then covered with *.+ mm thick silver polyto curtain. The +,, +. and +0-h treatment were covered likewise, and then exposed to 0*-Watt incandescent lamps, to receive

* **

Department of Agriculture, Faculty of Agriculture, Tokyo University of Agriculture

Department of Agricultural Science, Graduate School of Agriculture, Tokyo University of Agriculture

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supplemental light of ., 0 and 2-h respectively. The experiment was conducted in a greenhouse in which the minimum night temperature was maintained at +*ῌ.

Samples of three plants from each treatment were collected every six days from ,+ October, and the flower bud di#erentiation and development were ob-served using a stereoscopic microscope and a scanning electron microscope (SEM). For SEM, shoot apex samples were prepared, being fixed in ,ῌ glutaral-dehyde and osmiumic acid, serially dehydrated in eth-anol and critically point dried after substitution with isoamyl acetate, then vapor-deposited with Au-pd. A JSM-T,* SEM was used for observation.

Results

+. Vegetative growth

The e#ects of daylength on plant height are shown in Fig. +. No significant di#erences in the plant were found between the treatments of daylengths for /* to 0* days after sowing. Thereafter, the +0 and +.-h daylengths showed a marked e#ect on the height. In 2/ days after sowing, plant height on the +0-h daylength was the tallest, followed by the +.-h, +,-h, natural and 2 -h daylength in that order. The di#erence between the +0-h and +.-h daylength was small. The +0-h daylength was almost twice as tall as the 2-h daylength. The e#ects of daylength on the number of leaves are shown in Fig. ,. The number of leaves increased with the growth of plants regardless of daylength and had almost the same tendencies in each treatment. From

the above results, it was found that daylength greatly a#ects the plant height, while it has little influence on the number of leaves.

,. Daylength and Flower Bud Di#erentiation Table + and , shows the relationship between the daylength treatment and the di#erentiation and devel-opment of the flower buds. The influence of daylength on flower bud di#erentiation was obvious. The greater the given daylength was, the smaller the number of days from sowing to flower bud di#erentiation, result-ing in earlier flower bud di#erentiation. At -1 days from sowing, the +0-h daylength showed no flower bud di#erentiation. At .- days, however, it attained the predi#erentiation stage and the buds were already di#erentiated at .3 days. The +.-h daylength reached the flower bud di#erentiation stage at // days, the +,-h and natural daylength at 0+ days and 2-h daylength at 1-days. The plants under the +0-h were ,. days earlier than the plants under the 2-h.

The plant height at the flower bud di#erentiation stage was also compared. It was found that shorter daylength resulted in greater plant height at the di#er-entiation stage. The +0-h daylength showed flower bud di#erentiation when the height was +/ cm, while the 2 -h daylength was already ,0 cm tall when it reached the development stage. On the other hand, the plant height at the flowering stage was greater for longer daylength. The +0-h daylength was twice as tall as the 2-h daylength at the flowering stage. The number of leaves at the flower bud di#erentiation decreased as Fig. + E#ect of daylength on plant height of

coriander

Fig. , E#ect of daylength on number of leaves of coriander

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daylength increased. The +0-h daylength showed the smallest number of expanded leaves, followed by the +. -h, +,-h and natural, and 2-h daylength in that order. The +0-h and +.-h daylength showed similar number of leaves. The di#erentiation between the +0-h and 2-h daylength was ten leaves.

Similarly, the +0-h and +.-h daylength showed the smallest number of leaves at the flowering stage as well. The 2-h daylength showed the greatest number of leaves and the +,-h and natural daylength were an intermediate response. Daylength also influenced the growth of the plants after flower bud di#erentiation and longer daylength hastened bolting and flowering. The period from flower bud di#erentiation to flowering was the shortest for the +0-h daylength (,. days), followed by the +.-h daylength (,0 days), +,-h daylength (-* days) and 2-h daylength (,3 days). The greatest di#erence, however, between the treatments was as small as 0 days.

As the di#erence of period from flower bud di#eren-tiation to flowering was not considerable, it can be said that the di#erence of flowering date is mainly due to

the di#erence of the number of days from sowing to flower bud di#erentiation.

-. Development of Flower Buds

The development stage of flower buds is illustrated in Fig. - to ++. The shoot apices showed an apical dome shape. This stage is called the vegetative stage (Fig. -). After this stage, the apices were thickened and rounded up. This stage is the predi#erentiation period (Fig. .). The apices became flat and then both ends rose to develop two protuberances. Protuberances were also found in the axils. This stage is the cluster di#er-entiation stage (Fig. /). As clusters increased, lateral clusters in the axils also developed (Fig. 0). Clusters on the outer side of the axis developed faster compared to those on the inner side, some of which did not fully develop eventually. Then protuberances appeared on the top of the clusters. This is floret formation (Fig. 1). While floret increased, peduncles began to grow (Fig. 2). With regard to the development of florets, when the pedicels of florets had slightly grown, primary protu-berances of sepals were observed. Then inside the Table , E#ects of daylength on flower bud di#erentiation, bolting and flowering in coriander

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sepals, / primary protuberances of petals appeared (Fig. 3). At this stage, three of the five petals developed greatly while the growth of the remaining two slowed down. Later, five stamens appeared inside the petals (Fig. +*). At the same time, the center of the florets receptacles became flat and later developed two protu-berances. This is the di#erentiation of carpels (Fig. ++). When petals had fully developed, the protuberances in the floret center thickened and the carpels could be clearly identified.

The developmental scale of flower bud di#erentia-tion was referred after that in the celery by IWAMIet

al,ῌ

and ROELOFSEet al-ῌ, and the celeriac by BOOIJet al.ῌ.

The stage of flower bud development were classified as follows : +) vegetative stage ; ,) predi#erentiation stage ; -) cluster di#erentiation stage ; .) lateral cluster di#erentiation stage ; /) early stage of floret formation ; 0) later stage of floret formation ; 1) petal development stage ; 2) stamen development stage ; 3) carpel develop-ment stage.

Discussion

It was found that the growth and flower bud di#er-entiation are accelerated by long daylength and retarded by short daylength. Daylength also a#ected the growth after flower bud di#erentiation. Bolting, budding and flowering were also hastened by longer daylength. PUTIEVSKY/ῌ

conducted a study on the infl-uence of daylength on the growth and yield of corian-der and reported that longer daylength resulted in faster growth, greater yield and earlier flowering and maturing. In our study, where the minimum night temperature was maintained at +*῍, the results showed tendencies similar to those found in PUTIEVSKY’s observation.

With regard to the flower bud di#erentiation of umbellifers such as carrots and celeries, it has been known that flower buds are induced in plants that have reached a certain size and been exposed to low temper-ature for a certain period 0, 1, 2ῌ

. HAMNER and NAYLOR3ῌ

reported that dill was a typical long day plant which did not flower under daylength of ++-h or less but did flower under daylength +.-h or more. Also, NAYLOR+*ῌ

reported that the flower bud di#erentiation and bolting of dill were accelerated by long daylength. PUTIEVSKY/ῌ

found that the flowering and maturing of dill were hastened by high temperature, with the daylength con-dition maintained equal. KAWAI++ῌ

conducted a study on the flower bud di#erentiation and growth of Flor-ence fennel and reported that the longer the daylength was, the more accelerated the flower bud di#erentia-tion. Especially, the acceleration of flower bud

di#er-entiation was notable when the daylength was more than +, hours. Umbellifers can be classified into two types. For carrot and celery, flower bud di#erentiation is accelerated by exposure to low temperature. For dill and fennel, flower bud di#erentiation is accelerated by long daylength. Apparently, coriander belongs to the latter type. Although the flower bud di#erentiation was a#ected by day length, however, flowering occurred even under 2-h daylength. Therefore, corian-der can be consicorian-dered as a quantitative long day plant. Bolting of coriander tends to occur in high temperature and this is often a problem. Therefore, it can be as-sumed that temperature also a#ects flower bud di#er-entiation. IWAMIet al,ῌfound that the flower bud

di#er-entiation and bolting of celery di#ered among varieties. Therefore, di#erences among varieties must also be examined.

The stages of flower bud development were morphologically divided into nine stages. These stages were divided according to the results of studies con-ducted on celery-ῌ

and celeriac.ῌ

. Bracts did not devel-op, however, in the proximal of the pedicels and five small bracts were observed in the proximal on the florets. These bracts developed with the florets. Among the five petals, only three developed steadily and the development on the remaining two slowed down. These findings di#ered greatly from flower bud development of other umbellifers.

References

+ῌ PURSEGLOVE, J.W., BROWN, E.G., GREEN, C.L. and ROBBINS,

S.R. J., +32+. Spices. Vol. , : Longman. London. PP. ..1ῌ 2+-.

,ῌ IWAMI, T., KOONO, S. and TAKAHASHI, Y., +300. On develop-ment of flower bud and the time of flower bud di#eren-tiation and bolting among varieties of celeries. Abstr. Japan. Sci. Hort. Sci. Autumn Meet, +--ῌ+-..

-ῌ ROELOFSE, E.W., HAND, D.W. and HALL R.L., +323. The e#ect of daylength on the development of glasshouse celery. J. Hort. Sci. 0., ,2-ῌ,3,.

.ῌ BOOIJ, A., MEURS, E. J. J., THIEL, F. and BOESTEIN, A., +33,.

Cryo-scanning electron microscopy of the apex of celeriac (Apium graveolens L. var. rapaceum (Mill.) DC.) during initiation of the inflorescence. Scientia Hortic. /+, -*3ῌ-,*.

/ῌ PUTIEVSKY, E., +32-. E#ects of daylength and tempera-ture on growth and yield components of three seed spices. J. Hort. Sci. /2, ,1+ῌ,1/.

0ῌ INDEN, T., +3/*. Study on the flower bud di#erentiation

in carrot. Bull. Faculty Agr., Mie Univ. +, +.ῌ,+. 1ῌ THOMPSON, H.C., +3.*. Temperature in relation to

vegeta-tive and reproducvegeta-tive development. Proc. Amer. Soc. Hort. Sci. -1, 01,ῌ013.

2ῌ TSUKAMOTO, Y. and KUMAKI, Y., +3/1. Study on the flower bud di#erentiation in carrot. Bull. Res. Inst. Food Sci. Kyoto Univ. ,*, +-ῌ,-.

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responses of dill, a very sensitive long day plant. Bot. Gaz. +**, 2/-ῌ20+.

+*ῑ NAYLOR, A.W., +3.+. E#ects of some environmental factors on photoperiodic induction of beet and dill. Bot. Gaz. +*,, //1ῌ/1/.

++ῑ KAWAI, T. and ICHIKAWA, H., +322. Study on the cultural procedure in Florence fennel. II. On the factors and prevention of bolting. Abstr. Japan. Sci. Hort. Sci. Autumn Meet, +1*ῌ+1+.

コリアンダ

῏の花芽分化と発育に及ぼす日長の影響

富高弥一平*

ῌ狩俣貴清*ῌ野口有里紗**

ῐ平成 +- 年 / 月 -* 日受付ῌ平成 +- 年 3 月 ,* 日受理ῑ 要約 : コリアンダ῏の花芽分化と発育に及ぼす日長の影響について検討したῌ 日長は 2῍ +,῍ +.῍ +0 時間及び 自然日長とした草丈は長日ほど大きくなったが῍ 葉数は日長の影響をあまり受けなかったῌ 播種から花芽分化までの所要 日数は῍ +0 時間日長が最も短く .3 日であったῌ 一方῍ 2 時間日長は最も長く 1- 日であったῌ また῍ 花芽分 化から開花までの所要日数は長日ほど短くなったῌ 花芽分化時の展開葉数は長日ほど少なかったῌ 花芽分化は 2 時間日長でも見られたが῍ 長日ほど早くなかったῌ したがって῍ コリアンダ῏は花芽分化に 関しては῍ 量的長日植物と考えられるῌ 花芽の発育過程は次のように分けることができるῌ +῎ 未分化῍ ,῎ 分化初期῍ -῎ 花房分化期῍ .῎ 側花房分化期῍ /῎ 小花形成期῍ 0῎ 小花増加期῍ 1῎ 花弁形 成期῍ 2῎ 雄ずい形成期῍ 3῎ 雌ずい形成期ῌワῌド : コリアンダ῏῍ 日長῍ 発育῍ 花芽分化῍ 量的長日植物 * ** 東京農業大学農学部農学科 東京農業大学大学院農学研究科農学専攻

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