(A) (B)
(C)
Fig. 1
0 2 4 6 8
0 2 5 10 30 60
Time (min) ERK1/2 activity (fold increase)
AII 0 2 5 10 30 60 (min) ERK1/2
activity
Time (min) JNK activity (fold increase)
JNK activity
Src kinase activity
Time (min) Srckinaseactivity (fold increase)
AII 0 2 5 10 30 60 (min)
AII 0 2 5 10 30 60 (min)
0 10 20 30 40 50
0 2 5 10 30 60
0 10 20 30 40 50
0 2 5 10 30 60
IB: Phospho-Src
0 1 2 3
0 2 5 10 30 60
IB: Phospho-Src
0 1 2 3
0 2 5 10 30 60
Fig. 2
(A) (B)
(C) 0 2 4 6 8
0 0.01 0.1 1 10 100 1000 ERK1/2 activity (fold increase)
Concentration (nM) ERK1/2
activity
AII 0 0.01 0.1 1 10 100 1000 (nM)
0 20 40 50
0 0.01 0.1 1 10 100 1000 JNK activity (fold increase)
Concentration (nM) JNK
activity
AII 0 0.01 0.1 1 10 100 1000 (nM)
AII 0 0.01 0.1 1 10 100 1000 (nM) Src kinase
activity
0 1 2
0 0.01 0.1 1 10 100 1000 Srckinaseactivity (fold increase)
Concentration (nM)
30
10
3
Fig. 3
(A)
(B)
ERK1/2 activityOlm 0 100 0 0.1 1 10 100 (nM) AII (10 nM)
control
E R K 1/ 2 act iv it y (f o ld i n cr e ase)
AII (10 nM) Olm
control (nM)
0 2 4 6 8
100 0 0.1 1 10 100
0
** **
JNK activity
Olm 0 100 0 0.1 1 10 100 (nM) control AII (10 nM)
JN K act iv it y (f o ld i n cr e ase)
AII (10 nM) Olm
control (nM)
0 10 20 30 40 50
100 0 0.1 1 10 100
0
** **
(A)
(B)
ERK1/2 activitycontrol AII Olm PP2
AII
E R K 1/ 2 act iv it y (f o ld i n cr e ase) 0 2 4 6 8
Contol
AII
Olm PP2 AII Olm PP2
Olm PP2
**
**
JNK activity
JN K act iv it y (f o ld i n cr e ase) 0 10 20 30 40 50
Cont
AII
Olm PP2 AII Olm PP2
control AII Olm PP2
AII
Olm PP2
**
**
Fig. 5
control AII AII
+
Olm (100 µM)
C e ll m igr a tion (f o ld i n cr e ase)
AII (100 nM) (µM)
control 0
0.5 1 1.5 2 2.5 3
0 0.1 1 10 100
Olm 0
**
** **
*
AII (100 nM) (µM)
control 0
0.5 1 1.5 2 2.5 3
0 0.1 1 10 100
Olm 0
**
** **
*
c ont rol AI I Ol m U SP PP2 Ol m U SP PP2
AI I
Cell migration (fold increase)
** ** ** ** ** ** ** **
0 0.5 1 1.5 2 2.5 3