Radiation Biology Research Communications
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Transcriptional alteration and radiation effect in human induced pluripotent stem cells (iPSCs) and ectoderm tissues derived from iPSCs
Laboratory for Advanced Nuclear Energy, Institute of Innovative Research, Tokyo Institute of Technology,
Mikio Shimada*, Kaima Tsukada, Tomoko Miyake (Accepted for publication 23 December 2019)
Pluripotent Stem Cells: PSCs R N
Embryonic Stem Cells: ESCs Induced Pluripotent Stem Cells:
iPSCs C R N S i
S C 9 S C 9 C NS C 9
C N C 9 S R
PSCs C S N R DNA R
R D 9 S 9 9 9 P
S 9 PS
R D N
C D9 C N PSCs
Sw iPSCs9 A Sw iPSCs
A S R
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N1-30 2-12-1 Ookayama, Meguro-ku, Tokyo 152-8550 Japan
TEL: +81-3-5374-3703, FAX: +81-3-5734-3703, E-mail: [email protected]
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e iPS 9 9 9
Pluripotent stem cells (PSCs), such as embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), have potential to differentiate to the all organs. In general, radio-sensitivity is high in “cells have a greater reproductive activity”, “their karyokinetic fate is longer”, “their morphology and function are at least definitively fixed” as the low of Bergonié and Tribondeau. This rule applies very well to PSCs which have high radio-sensitivity and apoptosis activity, although DNA repair activity is also high. Mammalian organs separate two types, cell regeneration system (hematopoiesis, germline, epithelial, the crystalline lens) and cell non regeneration system (muscle, neural system). As similar with PSCs, organs of cell regeneration system are high radio-sensitivity compared with organs of cell non regenerative system. In this article, we discuss about relationships of differentiation dependent radio-sensitivity using our recent analysis with human iPSCs and derived neural stem cells and keratinocyte stem cells.
Key words: iPS cells, neural stem cells, keratinocytes, transcriptional alteration
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Pluripotent Stem Cells: PSCs
Embryonic Stem Cells: ESCs Induced Pluripotent Stem Cells:
iPSCs 4 (1, 2) PSCs
4 ESCs
ESCs ESCs 4 ESCs
leukemia inhibitory factor: LIF 9 4 ESCs
LIF 6 basic fibroblast growth factor: bFGF 9
3 9 6
09 4 3 5 ESCs
3.5 3 4.5 3 4 5.5 3 6.5
3 ESCs 9 4 5 9 EpiESCs Epiblast derived ESCs
9 EpiESCs LIF 6bFGF
9 3 ES EpiESCs 1 (3) 9 0
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4 2 PSCs 4 3
