Title
Combination Therapy of 5-Fluorouracil with Rapamycin for
Hormone Receptor-negative Human Breast Cancer( 内容の要旨
(Summary) )
Author(s)
細野, 芳樹
Report No.(Doctoral
Degree)
博士(医学) 乙第1448号
Issue Date
2010-11-17
Type
博士論文
Version
none
URL
http://hdl.handle.net/20.500.12099/36537
※この資料の著作権は、各資料の著者・学協会・出版社等に帰属します。
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Combination Therapy of 5-FLuorouracil with Rapamycin for Hormone Receptor-negative Human Breast Cancer
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4. Westernblotting :4}#BE(0. l-lOmM)5FU i RapamycinlOp M.#hn, Lift:/t&8=RIPAbuffer rC>*AS,
iETRf7*&/t&8= PVDF BS-0) transfer kWEt=T, -yj:btI*(mTOR, ERR, ART, p70S6K, caspase3,
cyclineBl, TS, OPRT), =yj:rnI4=n)fTyq8=blotting L,f=.
[#A]
l. Rapamycin 8=i 9 DNA %Bk8=b'V'TB;5St3:##Thb 9 , A,-3 5FU 0)##u]&rnJr&8=75,A,ba TS
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3: f= cyclin Bl 0)#gaEj: Rapamycin 0)ieL5i&#.r&8=tm& L,TU lf=.
2. Rapamycin 8=ct D ERR(Extracellular Signal-regulated Kinase) i ART 0) u y#1EbS#fRLf=.
bt&hlE#Hcatalase) *ilP8=i 9 ERR U y#1EEj:nS[J Snf=bS ART Ej:W$lJ5a17bA'-3f=o 3:f= ERR
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8j:5FU *i&(5. lj=0. 2 mM)8=tE L,T Rapamycin iqShp#(o. 25j=0. 03 mM) tj:#%8= (p4. 004)1ff:1#pC>b
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7iJ(E-7iet5nf=(^popticIndex : 0. 018 vs 0. 053). Rapamaycin ilfiW.T.Ej:caspase-3 0)7EJr&1Ek;;Ehb
tLL>fpJ3flo L,fp L,5FU i f(apamycin fJl)i..m1-7L)i 5r.'Uili4d]"iV)1tkf2]'iJit-(lmM i=W L/C 0.1mM)vC. caspasc3 A'it7-FfJrgilE:L, f=.
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mTOR kf#Bk+8 TORC2 b1 6 AKT I-tn 7 -I
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YOSHIKI HOSONO, SHINJI OSADA, MASAHITO NAWA, TAKAO TAKAHASHI, KAZUYA YAMAGUCHI, YOSHIHIRO
KAWAGUCHI and KAZUHIRO YOSHIDA : Combination Therapy of 5-Fluorouracil with Rapamycin
for Hormone Receptornegative Human Breast Cancer
Anticancer Research 30, 2625-2630 (2010)