s o t a S . C gemmifer ， T.Maeda and Uchino (

Mo l c a i g l o o p h r a n d chromosomal s e r u t a e f a n f o e w s e s i c e p ， S

c h o e n o p l e c t u

s o t a S . C gemmifer ， T.Maeda and Uchino (

C y p e r a c e a e

) .

March 2004

T e t s u y

a Maeda

G r a d u a t

e o l o c h S S f o e c n e i c a n d o g y h n o l T e c

KUMAMOTO UNIVERSITY

Chapter 3

V a r i a t i o

n S f o s u t c e l p o n e o h c gemmifer n i m

o r p h o l o g i c a

l comparison h t i w

S

. u s r o n a t m u c . r a v m u c r o n a t u s and S

. s u t a l u g n a i r t

3 .

1 n o i t c u d o r t n I

S c h o e n o p l e c t u

s ) b . h c R ( a l l a P s i one f t o e h d e a t g e g r e s r a e n e g from s u p r i c S . L . s L Cyperaceae a l l a P ( 1888a 、 b ， Goetghebeur . ) 8 9 9 1 r u o f - t y r i h T s e i c e p s and e e r h t s i d r b y h e r a known t n i e h s g e n u f o Ea t s Asia and North Am n a c i r e s n o i g c r (Hayasaka and s h i O h a α 2 a ) ( )

Smith and Hayasaka 2 ∞ ^{. 1 )}

S a t

o and Imae 0 ) 9 9 ( 1 found n unknown a t n a l p o f s t u c l e p n o e h o S c n i Aso Kyushu f o ， and d e t r o p e r t i s a “ " a r e i - K a n g a t a b e H s a e h t e s e a p a n J name. s h i T t n a l p d e r e f f i d from S

. n a t u s m u c r o . r a v t u s r o n a m u c and . S s u t a l u g n a i r t p n i s t n i o o f p s l i t s i

g v i n h a two d a e t s n i o f

28

t h r e

e . s a m g i t s t u b was d e t a l e r t o t e h two s e i c e p s n i e h t d e t f u t s u o r t e u q i r t c u l m s a n d s u o r t e u q i r t b

r a c t s

. The t n a l p was e t a i d e m r e t n i e n b e t w e e h t two s e i c e p s n i e h t a l c i o g l h o p o r m s r e t c a r a h c 、

s u c

h t s a e h h d t w i c f o u l m s ， h t g n e l a n d h d t w i s f o . s t e l e k i p h t g n e l a o f s r e h t n ， e d l k n i r w e c a f r u s o

f

n s t e l t u and e v i t a l e r h t g n e l p f o h t n a i r e n t e g m e s o t e l t u n . t However ， a l c g i o l h o p o r m - y l a n a s

e

s were t o n m e d f o r p e r n i i a t e d . l t o a S t e . l a ) 0 4 0 2 ( d e z n i g o e c r s i h t t n a l p a new s a s e i c e p s ， S

. e l i f e m m g ¥in s t n i o p p f o l i t s i h i t w two s a m g i t s ， r a e n i l f a e l e d a l b and . n o i t a r e f i l o r p The l

a t t e

r two s r e t c a r a h c ， however

d n e t d o t p l o e v e n t o e h s l a u d i v i d n i o w i n g g r n i i r 1 l s a n d s m a r e t s b

u

t do n t o a r e p a p y l t n e u q e r f n i l l i t s w a t 侃 Thus s l a ， u d i v i d n i S f o . m i f e r g e m t o u h t i w r a e n i l l

e a

f s e d a l b d a n n o i t a r e f i l o r p were c d u s n f c o h t i w c s o h t S o f . o n a t u s m u c r . r a v n a t u s u c r o m and S

. . s u t a l u g n a i r t I

n r e d r o o t c o n 白 nn t t a h . S gemmifer s r e f f i d from e h t r e h t o s e i c e p s n i a l c g i l o o p h r m o c

h a r a c t e r i s t i c

s a n i n o i t i d d t o t e h r a e n i l f a e l e d a l b and n o i t a r e f i l o r p ， we compared c 2 1 s r e t c a r a h b

y

means t f o e h l a c i t s i t a t s and e h t p i c n i r p I a component a n I a . s e s y

3 .

2 s l a i r e t a M and Methods

3 . 2 .

1 s l a i r e t a M

T h r e

e s e i c e p s t f o e h u s g e n s u t e c l o p n o e h c S ) a e e a c r p e C y ( ， . S gemm 件r . C o t a S ‘ T.Maeda

and Uchino ， . S

s u t a n r o c u ( し) a l l a P . r a v s n a t u u c r o m a n d . S s u t a l u g n a i r t ) . b x o R ( S吋 ι

were d s e u r o f e h t a l c g i o l h o p r m o . n t s m e r e s u m e a . S t u s m u c r o n a s a h e e r h t s e i t e i r a v h t i w - i r t q

u e t r o u

s m s c u l (Kohno t e u . l 200 . ) 1 The l a c i p y t y t e i r a v ， . r a v . u s t n a o c r m u s i t n e r e f f i d from v

a r

. s u n a r a t a t and . r a v e w a a z i h s i h n i g i n a v d w i n g e n o - s e l g n a t f o e h . m s l u c s l a i r e t a M were c

o l l e c t e

d from e h t n r e s l e w t r a p J f o a n a p e l b a T ( 1 . 3 a n d . g i F . ) 1 . 3 Some t f o r i e h s l a u d i v i d n i

were d e t a v i t l u c r o f more n a h t o n e r a e y n i e h t l a t n e m i r e p x e n d e r g a t a e h t y t l u c a F S f o e n c e c i ，

2

9

h a

d y l e s r e v s n a r t d l e n k i w r s e c a f r u s t ， l l b e s o h t S f o . s u t a l u g n a i r t h a d y l s l l o u c i p s n o c n i d e k l i n r w o

n e s .

3 . 3 .

2 s e s y l a n A t n i e h s l i t s i p

The number s f o s m a g i t (NST) d e i r a v f r o m one o t r u o f among . s l i t s i p s l i t s i P were e l b a t

o

be c d e i f i s s a l o t n i t h g i e s e p y t by t e h number d a n h t g n e l t f o e h s a m g i s t

t a h t s i ， one e p y t (M) i

n s l i t s i p t h i w a g m t i s e - o n ， two s e p y t . 0 ( and ) 2 0 n i a s g m i s t o - w t ， u r o f s e p y t . T ( o t

t n i - e e r h s

t i g m a

s a n d o n e e p y t ) e T ( f n i s a m g i t s - u r o . g i F ( . ) 5 . 3 The e c n e c s e r o l f n i f o s u c t e p l o n o e h S c was made from l a r e v e s s t e l e k i p s g v i n h a many s r e w o t f which d e r e f f i d n i e h t NST and l i t s i p e p y t from h a c e . r e w o t f s s l e e h t r e v e N ， e h t mode S f o . gemmifer was e p y t 1 0 8 . 4 8 ( %) g i n a v h two s

t i g m a

s s o f r a l i n r i h t g n e l e t i p s e d e h t e c e n r r c u c o s f o n e e v s e p y t ， t p e c x e r o f e h t s a m g i t s - r u o f

Te (

T a b l

e . 3 ) 3 t n I e h d e t a v i t l u c n o i t i d n o c ， y n l o e e r h t l i t s i p s e p y t S f o . gemm 伶r were d v e e r b s o and e p y t O . ( 7 . 2 9 %) was o s l a a n a . e g a t n v a d n [ t s a r t n o c ， . S a t u s u c r o n m a n d . S s u t a l u g n a i r t

were y t l a n i n o m d r e p e p y t T ， 8 ( 9 3 . % a n d . 4 5 8 ， % ) y l e v i t c e p s e r n i which e h t e e r h t s a m g i s t were o

f

s r a l i n r i . h t g n e l Types M and 02 were t o n n d f o u and e p y t 0 1 was d f o u n y I I a n o i t p e c x e n i a few s r e w o t f S f o . s u t a l u g n a i r t ・ from e h t n o i t a l l l p o p . T d i s T h was e l l r t r d e u n t h o b w I i d ( 8 . 0

%) a n d d e t a v i t l u c . 1 ( 1 %) . s n o i t i d n o c . u s T h . S gemm 俳r d d e e n t h o t a v e e p y t 1 0 s l i t s i p h i t w tw - o a s m g t i s t u b e h t r e h t o two s e i c e p s had e p y t w . T h i t . s a m g i t s - e e r h t

3 . 3 .

3 s e s y l a n A t n i e h h t n a i r e p s t n m e g e s

The s t l u s e r measurement f o o f NPS e r a shown n i l e b T a 3 . 4 . The s t e l t u n S f o . gemm 俳 r had x i s 9 . 6 4 ( %) s r o n e e v 5 . 5 4 ( %) h t n a i r e p t s m e n s e g n i e h t d l i w . n o i t i d n o c The modes h e o f t p

o p u l a t i o n

s Ng a n d Hb were x i s t u b e s o h t t o f e h s n o i t a l u p o p Zz and Sm were . n e v e s However ， i

n e h t d e t a v i t l u c n o i t i d n o c ， e h t modes a f o l l r u o f s n o i a t l u p o p were n v e e s h t n a i r e p s m e n t s e g

3

3

3 .

5 Acknowledgements

We w h i s e o t s s e r p x r u o s k n a h t o t o P r . f . r D i j u k a T o . i n s h H o The Okayama y t i s r e v i n U f o S

c i e n c

e ， r . M i h a s k a T u j i m a Y a s a n d r . M y a u t s T a Noguch . i members The f o e e s n a a p J y t e i c o S o

f

C . y o g l r o p e y r o f e h t g n i r e f f o m f o l a i r e t a . s t n a l p The t n e s e r p y d u t s was d t e r o p p u s n i p 訂 t by G d A i - n i - n t a r r o f c i f i t n e i c S r c h e a R e s Program ) c ( N o . 11640699 r o f A. Uchino from e h t J

a p a n e s

e y r t s i n i M f o Ed n o i t a c u ， e c e n i S c ， s t r o p S ， e r u t l u C and . g y o o l h n c T e

3

8

Chapter 4

S t a b i l i t

y and y t i l i b a i r a v chromosome f o

numbers n i s u t c e l p o n e o h c S gemm 約九

s

. a t u s r o n m u c . r a v mucron α s u t and

s . . s u t a l u g n a i r t

4 .

1 n o i t c u d o r t n I

The u s g e n s u c t e p l o e n o h S c ) . b h c R ( a l l a P s i o n e f o e h t d e t a g e r g e s a r e e n g from e h t polymo 中 c i h a n d c i t e l y h p y l o p e n u s g s u p r i c S . L . ¥ . s a n d s s e r i m p c o t u o b a 50 s e i c e p s w

i d e l

y d e t u b i r t s i d n i c h t l d w o r a y a s a k a ( H a n d u O h a s r 20 ∞ ^{. ) a . y l t n e c e R 出} e taxonomy w

i t h i

n s t u c l e p n o e o c h S was y l e v i t c a d e t a g i t s e v n i by Hayasaka a n d i h a s h O . p o ( ) . t i c a n d Smith a n d Hayasaka 1 0 2 0 ( ， 2 ∞ ^{. ) 2 They e d z i n g o e c r u r o f . s n o i t c e s c e s . t o p l h o e n S c}

s e

c . t o n a c o g e t M a l ( O h w i ) i t h . S m S . G a n d H a y a s a k a ， c e s . t n g e t o c t a e o A ) . b h c R ( l a n y a R . J and s

e

c . t i n i p u S . ) m e r C h ( l a n y a R . J ， n i e h t u s g e n . s u t c e l p o n e o h c S Moreover ， e h t new n o x t a n i 出 e

54

Acknowledgements

1 would e k i l o t s e s r p x e my s e r e c n i and d e e p e d u t i t a r g o t my s n g i s i v r e p u r o s s f e o r P . r D A

k i n o r

i Uchino r o f s i h t r p o p s u and s c e i v a d t u o h t g u r o h t my s . s e i d u t 1 am g l u f e t a r o t 出 e e

v a l u a t i o

n committe members: . f o r P . r D Akio a d i h s I ， r P . f o . r D Tadao k a . u s a a t s M . f o r P . r D o a s I Sanemasa and P r o . f . r D o r i j i n K u Yoshitama r o f r i e h t l a c i t i r c n t s m e s e s a s f my d o . n o i t a t r e s s i

My g e d u t i t a r s g o e o s l a D o t . r j i K a n Ono. . r D Masayuki Takamiya and r . M i s h y o h i C t o S a f

o

r r i e h t t r o p p u s n d a . e c i v d a J would e k i l acknowledge o t . f o r P . r D Y o s h i k a z I l Takada r o f s i h a

d v i c e

s t o u a b e h t l a c i t s i t a t s and e h t l a p i c n i r p component . s e s y l a n a

1 would o s l a e k i l o t acknowledge 1 a 1 o t f e h s r e h c a e t and members s o f f f a t t o f e h De- p

a r t m e n

t B f o l a c i g o l o i e e n c c i S ， e h t t e n n t r r p a e D f E o n t a l r o n m e n v i e n c i e S c and e h t G r a d u a t e S

c h o o

l S f o c e n i e c and Technology ， Kumamoto y t i s r e i v n U ， r o f r i e h t p l e h and . t r o p p u s

1 would e k i l acknowledge o t . f o r P . r D i j u k a T Hoshino ‘ The Okayama y t i s r e v i n U S f o - i c e

n c e

. M r . i s h k a T a Yas 吋 ima ‘ M r . Ta

uya Noguchi and . M r h i s o r i H . s a k i M a members f The o J

a p a n e s

e y t i e c o S f C o . o g y o l e r y p r o f e h t g n i r e f f o m f o s l a i r e t a and . M r h i s o r i H . i h u c i r o H e h t member f The o c s c a n a p J y e t i c o S o f Cyperology ， r o f s i h u s o i e c r p . s c c i v d a

1 wish a o t e k n o l e d g c . r D Yumi Kudo 、 e h t t h a l H e Care n t e r C e ， Kumamoto y i t s r e v i n U 、 r o f h

e

r c i r t a i h c y s p and l c a e d i m . t r o p p u s

1 would e k i l o t acknowledge e h t e t a l . r M Yasumitsu Maeda ， my g r e h t a f d n a r ， and e h t l

a t

e Mr. Yutaka Bekki ， e r h t a f d a n r g f my w o 江 e， Ryoko ， r o f r i e h t l l o i t c e f f a and g n i d n a t s r e d l l u

78