Title Sphingosine 1-phosphate (S1P) suppresses the collagen-induced activation of human platelets via S1P4 receptor( 図(Figure) )
Author(s) 大沼, 隆史
Report No.(Doctoral
Degree) 博士(医学) 甲第1060号
Issue Date 2018-03-25
Type 博士論文
Version ETD
URL http://hdl.handle.net/20.500.12099/75197
Figure 1
Laser Scattering Intensity (x102 V)
Time (min) Time (min) Time (min) Time (min)
panel 1 2 3 4
S1P (μM) 0 10 20 30
collagen + + + +
transmittance (%) 94 93 56 34
Ratio (%)
Small 32 31 53 67
Medium 18 21 28 22
Large 50 48 19 11
Total 100 100 100 100
3 2 1 0
120
0
% transmittance
0 5
0 5 0 5 0 5
Figure 2A
3 2 1 0
120
0
% transmittance
0 5 0 5 Laser Scattering Intensity (x 102 V)
Time (min) Time (min)
panel 1 2
S1P pretreatment - +
collagen + +
transmittance (%) 70 43
Ratio (%)
Small 35 64
Medium 29 24
Large 36 12
Figure 2B
3 2 1 0
120
0
% transmittance
0 5 0 5 Laser Scattering Intensity (x 102 V)
Time (min) Time (min)
panel 1 2
agent collagen collagen + S1P
transmittance (%) 87 65
Ratio (%)
Small 29 37
Medium 26 30
Large 45 33
Total 100 100
Lane S1P (μM) collagen 0 25 50 75 100 125
1 2 3 4
PDGF-AB (% of control)
Figure 3A
*
*
1 2 3 4
0 10 20 30
Lane S1P (μM) collagen 0 25 50 75 100 125
1 2 3 4
sCD40L (% of control)
Figure 3B
1 2 3 4
0 10 20 30
+ + + +
*
*
Lane S1P collagen
1 2
- +
0 25 50 75 100 125
phospho-HSP27 (Ser-78) (% of control)
Figure 3C
*
0 1 2 3 4
1 2 3 4
Lane
Figure 4A
phospho-HSP27 (Ser-78) GAPDH Lane S1P collagen
* **
1 2 3 4
- - + +
- + - +
Fold increase
0 1 2 3
0 1 2 3
Lane
Figure 4B
phospho-p38 GAPDH Lane S1P collagen
* **
1 2 3 4
- - + +
- + - +
Fold increase
Figure 5A
1 2
- +
0 30
GTP-Gαi Gαi Lane S1P Time (sec)
Figure 5B
1 2
- +
0 30
GTP-Gα13 Gα13 Lane S1P Time (sec)
Figure 6A
3 2 1 0
120
0
% transmittance
0 5 0 5 Laser Scattering Intensity (x 102 V)
Time (min) Time (min)
panel 1 2
SEW2871 (μM) 0 10
collagen + +
transmittance (%) 86 84
Ratio (%)
Small 29 30
Medium 19 19
Large 52 51
Total 100 100
Figure 6B
3 2 1 0
120
0
% transmittance
0 5 0 5 Laser Scattering Intensity (x 102 V)
Time (min) Time (min)
panel 1 2
CYM50260 (μM) 0 25
collagen + +
transmittance (%) 81 62
Ratio (%)
Small 35 50
Medium 22 22
Large 43 28
1 2 3
- + -
- - +
+ + +
0 25 50 75 100 125
PDGF-AB (% of control)
Figure 7A
Lane SEW2871 CYM50260 collagen
N.S. *
Lane SEW2871 CYM50260
1 2 3
- + -
- - +
+ + +
0 25 50 75 100 125 150
sCD40L (% of control)
Figure 7B
N.S. *
Lane CYM50260 collagen
1 2
- +
+ +
0 25 50 75 100 125
phospho-HSP27 (Ser-78) (% of control)
Figure 7C
*
Lane 0 0.5 1.0 1.5
Fold increase
Figure 8A
1 2
- +
+ +
N.S.
phospho-HSP27 (Ser-78) GAPDH Lane SEW2871 collagen
Lane 0 0.5 1.0 1.5
1 2
Fold increase
Figure 8B
* phospho-HSP27 (Ser-78)
GAPDH Lane CYM50260 collagen
1 2
- +
+ +
0 1 2 3 4 5
Figure 9A
phospho-HSP27 (Ser-78) GAPDH Lane CYM50358 collagen
N.S.
1 2 3 4
- - + +
- + - +
Fold increase
Lane 0 2 4 6
1 2 3 4
Fold increase
Figure 9B
phospho-HSP27 (Ser-78) GAPDH Lane CYM50358 S1P collagen
1 2 3 4
- - - +
- - + +
- + + +
* **
N.S.
