200 Nippon Shokuhin Kagaku Kogaku Kaishi Vol. /0, No..,,**,*2 (,**3) 8 Salmonella Enteritidis Inhibitors of Adhesion Ability of Salmonella Enteritidis

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(1)200. Nippon Shokuhin Kagaku Kogaku Kaishi Vol. /0, No. ., ,**,*2 (,**3) . 8 . Salmonella Enteritidis ῌῑῐ῏῍ῒ῎ ³T´µ U¶ · ^VW¸ ¹º»XY T¼½y ! "#!$% &'()* +,&-. . Inhibitors of Adhesion Ability of Salmonella Enteritidis Takahisa Miyamoto, Joh Kawaguchi, Satoshi Shimotsu, Johtaro Kawagishi and Ken-Ichi Honjoh Laboratory of Food Hygienic Chemistry, Division of Food Biotechnology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 0ῌ+*ῌ+, Hakozaki, Higashi-ku, Fukuoka, 2+,ῌ2/2+ ῍῍῍῍῍῍῍῍῍῍῍῍῍῍῍῍ Inhibitors of Salmonella Enteritidis adhesion to microplates were screened among -3 additives including natural and food additives known to be highly safe. S. Enteritidis adhered strongly to microplates after incubation in *.+ῌ Bacto-soytone for ,. h. Adhesion was inhibited in the presence of pigments (Annatto pigment, Gardenia yellow, and Monascus pigment), food coloring products (Annatto AN, San-brown AC, San-yellow No. ,AU, San-red YM, San-red RCFU, and San-beet L), food additives (protamine, Chili extract, sucrose fatty acid esters, and monoglycerine fatty acid esters), and a flavonoid (Kaempferol). Among them, Annatto AN, Gardenia yellow, Monascus pigment, and sugar fatty acid esters inhibited adhesion at concentrations below antibacterial activity. In the case of sugar fatty acid esters of C2 to C+2, the longer the fatty acid chain the stronger the inhibitory e#ects. Monascus pigment and sucrose monostearate were the strongest inhibitors of S. Enteritidis adhesion, even at *.*+ῌ without antibacterial activity. (Received Sep. 2, ,**2 ; Accepted Dec. ,., ,**2) Keywords : Salmonella, adhesion, inhibitor, sugar fatty acid ester, Monascus pigment. ῍ῌ῏ῌ῎ :

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(78) +1 /1 3 22ῌ+1. ,**0 , +./.- Description of the dose-response relationship In “Microbiological Risk Assessment Series +, Risk Assessments of Salmonella in Eggs and Broiler Chickens”, eds World Health Organization, Food and Agriculture Organization of the United Nations, (FAO/WHO), pp. --ῌ -/ (,**,). - ,3 +*1ῌ++. ,**+ . Solano, C., Garcia, B., Valle, J., Berasain, C., Ghigo, J.M., Gamazo, C. and Lasa, I., Genetic analysis of Salmonella enteritidis biofilm formation : critical role of cellulose. Mol. Microbiol., .-, 13-ῌ2*2 (,**,). / Gerstel, U. and Romling, U., The csgD promoter, a control unit for biofilm formation in Salmonella typhimurium. Research in Microbiology, +/., 0/3ῌ001 (,**-). 0 Collinson, S.K., Emody, L., Trust, T. J. and Kay, W.W., Thin aggregative fimbriae from diarrheagenic Escherichia coli. J. Bacteriol., +1., ..3*ῌ..3/ (+33,). 1 Prigent-Combaret, C., Brombacher, E., Vidal, O., Ambert, A., Lejeune, P., Landini, P. and Dorel, A., Complex Regulatory Network Controls Initial Adhesion and Biofilm Formation in Escherichia coli via Regulation of the csgD Gene. J. Bacteriol., +2-, 1,+-ῌ1,,- (,**+). 2 ! !"#$%& "'( !"#$)# *$+% NTS &, pp. +,-ῌ+-, ,**2 3 Medina, M.B. and Fratamico, P.M., Binding interactions of collagen I, laminin and fibronectin with immobilized Escherichia coli O+/1 : H1 using a surface plasmon resonance biosensor. Biotechnology Techniques, +,, ,-/ῌ,.* (+332). +* Fratamico, P.M., Schultz, F. J., Benedict, R.C., Buchanan, R.L. and Cooke, P.H., Factors influencing attachment of Escherichia coli O+/1 : H1 to beef tissues and removal using selected sanitizing rinses. Journal of Food Protection, /3, ./-ῌ./3 (+330). ++ Miyamoto,T., Shimizu, Y., Kobayashi, H., Honjoh, K. and Iio, M., Studies of collagen binding with immobilized Salmonella enteritidis and inhibition with synthetic and naturally occurring food additives by a surface plasmon resonance biosensor. Sensors and Materials, +/, ./-ῌ.00 (,**-). +, Mireles, II, J., Toguchi, A. and Harshey, R.M., Salmonella enterica serovar Typhimurium swarming mutants with altered biofilm-forming abilities : Surfactin inhibits biofilm formation. J. Bacteriol., +2-, /2./ῌ/2/. (,**+). +- Jonas, K., Tomenius, H., Kader, A., ,, Normark, S., R¨ omling, U., Belova, L.M. and Melefors, » O., Roles of curli,.

(79) 208. +. +/. +0. +1. +2. =>)() /0 . ,**3 . cellulose and BapA in Salmonella biofilm morphology studied by atomic force microscopy, BMC Microbiology, 1 : 1* doi : +*.++20/+.1+-,+2*-1-1* (,**1). This article is available from : http : //www.biomedcentral.com/+.1+,+2*/1/1*. *+,- .

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