634 Nippon Shokuhin Kagaku Kogaku Kaishi Vol. /-, No.+,, (,**0) 38 PCR Bienvenido O. Juliano* * Philippine Rice Research Institute Cultivar Id

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(1)634. Nippon Shokuhin Kagaku Kogaku Kaishi Vol. /-, No. +,, 0-.}0.- (,**0) »";¼. & 38 (. PCR

(2) . Bienvenido O. Juliano*

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(5) * Philippine Rice Research Institute. Cultivar Identification and Palatability Estimation of +. Typical Philippine Rice Cultivars by the PCR Method Sumiko Nakamura, Keitaro Suzuki, Kazutomo Haraguchi, Yoko Takemoto, Bienvenido O. Juliano* and Ken’ichi Ohtsubo National Food Research Institute, ,῍+῍+,, Kannondai, Tsukuba, Ibaraki, -*/῍20., *Philippine Rice Research Institute, Maligaya, Munoz, Nueva Ecija, Philippines ῍῍῍῍῍῍῍῍῍῍῍῍῍῍῍῍ We have developed five PCR-based DNA markers for rice blast resistance. All the DNA markers developed in the present study were shown to be located on the same chromosomes as the target blast resistance genes and are estimated to be closely linked with them. Using PCR for these primers, four typical Philippine rice cultivars were found to have di#erent DNA patterns. Furthermore, we have developed a novel primer closely related to soluble starch synthase (SSSῌ). This primer revealed marked di#erences between Japonica and Indica subspecies of rice. In addition, the results of PCR were highly correlated with the amylose content. This is due to the close location of granule-bound starch synthase (GBSS) and SSῌ on rice chromosome six at an approximate map distance of /cM. The PCR results for +. typical Philippine rice cultivars also showed high correlation for the amylose content. The present authors developed a formula for estimating the amylose content and gel consistency by multiple regression analyses based on the results of PCR as the explanatory variables. (Received Apr. ,+, ,**0 ; Accepted Sep. ,2, ,**0). !" 0 #$% &'( )*+ ,-,. ) G 1

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(152) ©ª± . QTL ²« + >w +C -2 >+333C +1C Fukuta, Y., Yanoria, M. J.T., Ebron, L.A., Mercado, D.M. and Khush G.S., Genetic and breeding analysis using molecular marker,,,.Reaction pattern of QTL for Philippines blast Isolate, Breeding Research, - (Suppl), .0 >,**+C +2C n³ ´¬µK2Z® ´¬µd ¯. .- ¶·- 00 ,*ῌ,/ >+332C +3C Baba, T., Nishihara, M., Mizuno, K., Kawasaki, T., Shimada, H., Kobayashi, E., Ohnish, S., Tanaka, K. and Arai, Y., Identification cDNA cloning and gene expression of soluble starch synthase in rice (Oryza sativa. L).

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(154) -3 0 /*2ῌ/+- +33, Williams, J.G.K., Kubelik, A.R., Livak, K. J. and Tingey, S.V., DNA polymorphisms amplified by arbitrary primers are useful as genetic markers, Nucleic Acids Research, +2, 0/-+ῌ0/-/ +33* ! "ῌ#$ % &'()*+,-./0123 4&'56789:;<=> Pii ?@ABCDE F GH I / J + +*2 ,**- Jia. Y., Wang, Z., Singh, P., Development of dominant rice blast Pi-ta resistance gene markers, Crop Science, .,, ,+./ῌ,+ ,**,. ,1. ,2. ,3. -*. -+ -,. 643. Hayashi, K., Hashimoto, N., Daigen, M., Ashikawa I., Development of PCRKbased SNP markers for rice blast resistance genes at the Piz locus, Theor Appl Genet, +*2, +,+,ῌ+,,* ,**. LM N #$OP QRS TUVW XYZ[ \ $] ^_`Gabcdefabcghijkl m DH nop0254qirlm QTL st FGH I .2 J , +33, Jiang, H.W., Dian, W.N and Wu, P., Oryza sativa (indica cultivar-group)putative soluble starch Synthase I gene, NCBI ACCESSION AY,33.*. ,**- Tanaka, K., Ohnishi, S., Kishimoto, N., Kawasaki, T. and Baba, T., Structure organization and chromosomal location of the gene encoding a form of rice soluble starch synthase, Plant Physiol, +*2, 011ῌ02- +33/ Muvw xeyz{ |}H~e H / , pp. +.2ῌ+/- +321 ! - &'5678i3 FG H I / +1 ,**-. +2 . ,+ +2 3 ,2 . ῍῍῍῍῍῍῍῍῍῍῍῍῍῍῍῍῍῍῍῍῍῍῍῍.

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