(Summary) ) Author(s) Shaoxia PU Report No.(Doctoral Degree) 博士(獣医学) 甲第521号 Issue Date 2019-03-13 Type 博士論文 Version none URL http://hdl.handle.net/20.500.12099/77952 ※この資料の著作権は、各資料の著者・学協会・出版社等に帰属します。
Ặྡ㸦ᮏ㸦ᅜ㸧⡠㸧 6KDR[LD38㸦୰⳹ேẸඹᅜ㸧 ᣦ ᑟ ᩍ ဨ Ặ ྡ ᮾி㎰ᕤᏛ ᩍᤵ Ώ ㎶ ඖ Ꮫ ࡢ ✀ 㢮 ༤ኈ㸦⋇་Ꮫ㸧 Ꮫ グ ␒ ྕ ⋇་༤⏥➨㸳㸰㸯ྕ Ꮫ ᤵ ᖺ ᭶ ᪥ ᖹᡂ㸱㸯ᖺ㸱᭶㸯㸱᪥ Ꮫ ᤵ ࡢ せ ௳ Ꮫつ๎➨㸲᮲➨㸯㡯ヱᙜ ◊ ✲ ⛉ ཬ ࡧ ᑓ ᨷ 㐃ྜ⋇་Ꮫ◊✲⛉ ⋇་Ꮫᑓᨷ ◊✲ᣦᑟࢆཷࡅࡓᏛ ᮾி㎰ᕤᏛ Ꮫ ㄽ ᩥ 㢟 ┠ 7KH,QIOXHQFHRI+LJK7HPSHUDWXUHRQ5HSURGXFWLRQ DQG(IIHFWRI6XJDU&DQH([WUDFWLQWKH-DSDQHVH 4XDLO ࢽ࣍ࣥ࢘ࢬࣛࡢ⦾Ṫᑐࡍࡿᬬ⇕ࡢᙳ㡪ࢧࢺ࢘࢟ࣅ ࢚࢟ࢫࡢຠᯝ㸧 ᑂ ᰝ ጤ ဨ ᰝ ᮾி㎰ᕤᏛ ᩍᤵ బࠎᮌ ୍ ᰝ ᖏᗈ␆⏘Ꮫ ᩍᤵ ᐊ ႐ ᬒ ᰝ ᒾ ᡭ Ꮫ ᩍ ᤵ 㧗 ᶫ ㏱ ᰝ ᮾி㎰ᕤᏛ ᩍ ᤵ Ώ ㎶ ඖ ᰝ ᒱ 㜧 Ꮫ ᩍᤵ ᳝ ྡ ㈗ ᙪ Ꮫㄽᩥࡢෆᐜࡢせ᪨ ኟᏘࡢᬬ⇕ࢫࢺࣞࢫࡼࡗ࡚⦾Ṫ㞀ᐖࡀ㉳ࡁࡿࡇࡀࡼࡃ▱ࡽࢀ࡚࠸ࡿࡀ㸪ࡑࡢ࣓࢝ࢽ ࢬ࣒ࡘ࠸࡚ࡣ᫂࡞㒊ศࡀከ࠸ࠋᮏ◊✲࡛ࡣ㸪ᬬ⇕ࡼࡿ༸⬊Ⓨ⫱ᢚไࡢ࣓࢝ࢽࢬ࣒ࢆ ෆศἪᏛⓗ㸪࠾ࡼࡧᰤ㣴Ꮫⓗゎᯒࡋࡓࠋ ࢧࢺ࢘࢟ࣅ࢚࢟ࢫ㸦SCE㸧ࡣ, ࢧࢺ࢘࢟ࣅࡢ⤠ࡾỒࡽࣈࢻ࢘⢾, ᯝ⢾, ࢩࣙ⢾ࢆศ㞳ࡋ ࡓṧࡾ࡛, ᢠ⅖ཬࡧᢠ㓟⏕≀άᛶ≀㉁ࢆྵࡳ, ࡍ࡛ື≀ࡢ㣫ᩱῧຍ≀ࡋ࡚ࢃࢀ ࡚࠸ࡿࠋᮏ◊✲࡛ࡣ, 㞤㞝ࡢᡂ⇍࢘ࢬࣛࢆ⏝࠸࡚, ࣍ࣝࣔࣥ⃰ᗘ, ᛶ⭢ࡢࢫࢸࣟࢻྜᡂ ᑐࡍࡿᬬ⇕ࡢᙳ㡪ࡘ࠸࡚SCE ᢞࡢຠᯝࢆゎᯒࡋࡓࠋ ➨2 ❶࡛ࡣ, ᡂ⇍㞤࢘ࢬࣛࢆ 10 ᪥㛫㐃⥆ࡋ࡚㧗 ᭀ㟢ࡋ㸪༸ࡢ㉁㛵ࡍࡿᙳ㡪ࢆゎ ᯒࡋࡓࠋࡑࡢ⤖ᯝ㸪ᬬ⇕ฎ⌮⩌࡛ࡣ༸㔜㔞ࡀ᪥ẖᚎࠎῶᑡࡋ㸪⏘༸⋡ࡶ᭷ពῶᑡࡋ ࡓࠋᬬ⇕ฎ⌮⩌࡛ࡣ༸ᕢ㔜㔞ཬࡧ༸⟶㔜㔞ࡀ᭷ពῶᑡࡋࡓࠋࡲࡓ㸪㯤Ⰽ༸⬊ࡢᩘ༸ᕢ 㔜㔞ࡶῶᑡࡋࡓࠋ୍᪉㸪⾑ᾮ୰ࢥࣝࢳࢥࢫࢸࣟࣥ⃰ᗘࡣᬬ⇕ฎ⌮ࡼࡗ࡚᭷ពቑຍࡋ㸪 ༸㯤୰ࢥࣝࢳࢥࢫࢸࣟࣥ⃰ᗘࡶ㯤Ⰽ༸⬊㸦F1, F2, F3㸧࡛᭷ពቑຍࡋࡓࠋࢫࢸࣟࢻྜ ᡂ㓝⣲ࡢ୍ࡘ࡛࠶ࡿ17ș-HSD 㑇ఏᏊࡢ༸ᕢ࡛ࡢⓎ⌧ࡣኚࡀぢࡽࢀ࡞ࡗࡓࡀ㸪⭈ ࡛ࡣ᭷ពቑຍࡋࡓࠋ௨ୖࡢ⤖ᯝࡣ㸪ᬬ⇕ฎ⌮ࡼࡾ⭈࠾࠸࡚ࢫࢸࣟࢻྜᡂ㓝⣲ࡢ 㑇ఏᏊⓎ⌧ࡀቑຍࡋ㸪ࡑࡢ⤖ᯝ༸㯤ࡢࢥࣝࢳࢥࢫࢸࣟࣥࡢ✚ࡀቑຍࡋࡓࡇࢆ♧၀ࡋ ࡓࠋ ➨3 ❶࡛ࡣ㸪ᬬ⇕ฎ⌮ᙳ㡪ࡢ࣓࢝ࢽࢬ࣒ࢆ᫂ࡽࡍࡿࡓࡵ㸪⾑Ύ୰௦ㅰ≀ྵ᭷㔞ࢆ ゎᯒࡋࡓࠋ⾑Ύ୰165 ࡢ௦ㅰ≀ࡀ᳨ฟࡉࢀ㸪ࡑࡢෆࡢ 7 ࡘࡘ࠸࡚ᑐ↷⩌ᬬ⇕ฎ⌮⩌ 㸦㸲㸧
ࡢ㛫࡛᭷ព࡞ᕪࡀㄆࡵࡽࢀࡓࠋゎᯒࢯࣇࢺMetaboAnalyst ࡼࡗ࡚ゎᯒࡋࡓࡇࢁ㸪ᬬ⇕
ฎ⌮ࡀbutanoate, propanoate, ࠾ࡼࡧ pyrimidine ࡢ௦ㅰ㸪ࢣࢺࣥయࡢྜᡂศゎ㸪
cyanoamino acid ࡢ௦ㅰᙳ㡪ࢆ࠼࡚࠸ࡓࡇࡀ♧ࡉࢀࡓࠋࡉࡽ㸪ᑠ⭠⫢⮚ࢆ⤌⧊Ꮫ ⓗゎᯒࡋࡓ⤖ᯝ㸪༑ᣦ⭠㸪✵⭠,ᅇ⭠ࡢ⤧ẟࡢ㧗ࡉࡀῶᑡࡍࡿࡇࢆ᫂ࡽࡋࡓࠋ ⫢⮚ࡢ୰ᛶ⬡⫫, ࢥࣞࢫࢸ࣮ࣟࣝྵ㔞ࡀቑຍࡋࡓࡀ㸪⾑ᾮ୰ࢥࣞࢫࢸ࣮ࣟࣝࣞ࣋ࣝࡣపୗ ࡋࡓࠋ⫢⮚࠾ࡅࡿ⬡㉁௦ㅰ㛵㐃ࡋࡓ㑇ఏᏊࡢⓎ⌧㔞ࡀ᭷ពኚࡋࡓࠋ௨ୖࡢ⤖ᯝ ࡽ㸪ᬬ⇕ฎ⌮ࡀᑠ⭠㸪⫢⮚ࡢ㞀ᐖࢆ㉳ࡇࡋࡓ⤖ᯝࡋ࡚㸪⬡㉁௦ㅰࡀᙳ㡪ࢆཷࡅࡓ⪃࠼ ࡽࢀࡓࠋ ➨4 ❶࡛ࡣ㸪20 ᪥㛫ࡢᬬ⇕ฎ⌮ࡢᙳ㡪ࡘ࠸࡚ゎᯒࡋࡓࠋᡂ⇍ࡋࡓ㞤࢘ࢬࣛࢆ⏝࠸㸪 ⏕Ṫᶵ⬟ࡢᙳ㡪ࢆゎᯒࡋࡓ⤖ᯝ㸪ᬬ⇕ฎ⌮ࡼࡗ࡚㸪༸ᕢ㸪༸⟶㔜㔞ࡶ᭷ពῶᑡ ࡋ㸪㯤Ⰽ༸⬊ᩘ㸪㔜㔞ඹῶᑡࡋ, ༸㔜㔞ࡀῶᑡࡋࡓࠋཷ⢭⋡ࡣኚࡀ࡞ࡗࡓࡀ, ᬬ ⇕ฎ⌮⩌࡛, ึ⏕㞮ࡢయ㔜ࡀ᭷ពῶᑡࡋࡓࠋ⾑୰ࢥࣝࢳࢥࢫࢸࣟࣥ⃰ᗘ㸪࢚ࢫࢺࣛࢪ࢜ ࣮ࣝ -17ș⃰ᗘࡀࡕࡽࡶቑຍࡋࡓࠋ༸㯤୰ࡢࢥࣝࢳࢥࢫࢸࣟࣥ⃰ᗘ㸪࢚ࢫࢺࣛࢪ࣮࢜ࣝ-17ș⃰ᗘࡶྠᵝࡢഴྥࢆ♧ࡋࡓࠋ༸ᕢࢫࢸࣟࢻྜᡂ㓝⣲ P450scc, ࢚ࢫࢺࣟࢪ࢙ࣥ ࣞࢭࣉࢱ࣮(ER)ࡢ㑇ఏᏊⓎ⌧ࡀୖ᪼ࡋࡓࠋࡋࡋ࡞ࡀࡽ㸪FSH ࡢࣞࢭࣉࢱ࣮ࡢ㑇ఏᏊⓎ⌧ ࡣῶᑡࡋࡓࠋ௦ㅰゎᯒࡢ⤖ᯝ, ⾑Ύ୰ 165 ࡢ௦ㅰ≀ࡀ᳨ฟࡉࢀ, 8 ࡘࡢ௦ㅰ≀᭷ព࡞ᕪ ࡀㄆࡵࡽࢀࡓࠋMetaboAnalyst ࡛ࡢศᯒ⤖ᯝ, propanoate ௦ㅰ, beta-alanine ௦ㅰ, aspartate histidine ௦ㅰࡶᙳ㡪ࡍࡿࡇࡀ᫂ࡽ࡞ࡗࡓࠋ⫢⮚ࡢ⤌⧊ゎᯒࡢ⤖ᯝࡣᬬ⇕ฎ⌮ࡀ⫢
⮚ࡢ⬡㉁௦ㅰ␗ᖖࢆ㉳ࡇࡍࡇࢆ♧ࡋࡓࠋ୰ᛶ⬡⫫, ࢥࣞࢫࢸ࣮ࣟࣝࡀ⫢⮚࡛ቑຍࡋ,
AGPAT5 mRNA ࡀቑຍࡋ࡚࠸ࡓࠋࡉࡽ, ⫢⮚࡛࣏ࢺ࣮ࢩࢫ㛵㐃ࡋࡓ caspase3 ࡢ㑇ఏ ᏊⓎ⌧, ࢧࢺ࢝ࣥࡢ IL-6 TLR4 ࡢ㑇ఏᏊⓎ⌧ࡀቑຍࡋ࡚࠸ࡓࠋNF-ȡB ࡢ㑇ఏᏊ Ⓨ⌧ࡣῶᑡࡋ࡚࠸ࡓࠋ௨ୖࡢ⤖ᯝࡽ㸪ᬬ⇕ฎ⌮ࡀ⫢⮚ࢆയᐖࡋ, ⬡㉁௦ㅰࡀᙳ㡪ࢆཷ ࡅ㸪apoptosis ⅖ࡀ⏕ࡌࡓุ᩿ࡉࢀࡓࠋ⾑ᾮ୰࢚ࢫࢺࣛࢪ࣮࢜ࣝ-17șࡢ⫢⮚࡛ࡢ௦ ㅰࡶയᐖࡉࢀ, ༸ᕢࡢᶵ⬟, 㯤Ⰽ༸⬊ࡢῶᑡ, 㔜㔞ῶᑡ, ༸㔜㔞,ึ⏕㞮య㔜ࡢῶᑡ࡞ ࢆᘬࡁ㉳ࡇࡋࡓ⪃࠼ࡽࢀࡓࠋ ➨5 ❶࡛ࡣ, ᬬ⇕ฎ⌮ᑐࡍࡿ SCE ࡢຠᯝࢆ㸪㞤㞝࢘ࢬࣛࢆ⏝࠸࡚ゎᯒࡋࡓࠋ㣵ᩱ SCE ࢆῧຍࡋࡓ⤖ᯝ, 㞤࡛ࡣᬬ⇕ฎ⌮ࡼࡿ༸㔜㔞ࡢῶᑡࢆࢃࡎ⦆ࡋࡓࠋ༸ᕢ, ༸ ⟶㔜㔞ࡀ6 ୰ 2 ࡛ῶᑡࡋࡓࠋࡑࡢ⾑୰ࢥࣝࢳࢥࢫࢸ࢚ࣟࣥࢫࢺࣛࢪ࣮࢜ࣝ 17ș⃰ ᗘࡀῶᑡࡋࡓࠋ୍᪉㸪༸ᕢ࡛ࡢࢫࢸࣟࢻྜᡂ㓝⣲P450scc ࡢ㑇ఏᏊⓎ⌧ࡀᬬ⇕ฎ⌮ᚋ ቑຍࡋࡓࠋ0.02㸣ࡢ SCE 㣫ᩱῧຍࡣ༸⏕⏘ࢆቑຍࡋࡓࡀ, P450scc ࡢ㑇ఏᏊⓎ⌧ࡶᬬ⇕ฎ ⌮ࡼࡗ࡚ቑຍࡋࡓࠋᅇ⏝ࡋࡓ⃰ᗘࡢSCE ᦤྲྀࡢᬬ⇕ฎ⌮ᑐࡍࡿຠᯝࡣ㝈ᐃⓗ࡛, ࡉࡽ࡞ࡿ᳨ウࡀᚲせ࡛࠶ࡿ⪃࠼ࡽࢀࡓࠋSCE ᢞࡀ㞝࢘ࢬࣛࡢᛶ⾜ື⢭ᕢᶵ⬟࠼ ࡿస⏝ࢆ᳨ウࡋࡓ⤖ᯝ, ⾑୰ࢸࢫࢺࢫࢸࣟࣥ⃰ᗘࡀῶᑡࡋ㸪ᑿ⾜ືࡀῶᑡࡋࡓࠋ⢭ᕢࡢ ࢫࢸࣟࢻྜᡂ㓝⣲P450c17, 17șHSD, P450scc, 3șHSD ࡢ㑇ఏᏊⓎ⌧ࢆゎᯒࡋࡓ⤖ᯝ, ࠸ ࡎࢀࡶῶᑡࡋࡓࠋ⺯ගච⤌⧊ᏛᰁⰍ࢚࢘ࢫࢱࣥࣈࣟࢵࢸࣥࢢἲ࡛⢭ᕢࡢ3șѸHSD ࢆゎᯒࡋࡓ⤖ᯝ, SCE ᢞ࡛࠸ࡎࢀࡶῶᑡࡋ࡚࠸ࡓࠋ⢭ᕢࡢ㛫㉁⣽⬊ࢆᇵ㣴ࡋ, SCE ࢆ ࣄࢶࢪLH ࡶᇵ㣴ᾮῧຍࡋࡓ⤖ᯝ, ࢸࢫࢺࢫࢸࣟࣥศἪ, 3șHSD ࡢ㑇ఏᏊⓎ⌧ ࡶ, ῶᑡࡋࡓࠋSCE ࡣ┤᥋⢭ᕢࡢ㛫㉁⣽⬊స⏝ࡋ, 3șHSD ࡢⓎ⌧ࢆᢚไࡋ࡚, ⢭ᕢᶵ ⬟ࢆᢚไࡍࡿ⪃࠼ࡽࢀࡓࠋ ᬬ⇕ࢫࢺࣞࢫࡼࡗ࡚ᐙ⚺ࡢ⦾Ṫ㞀ᐖࡀ㉳ࡁࡿ࣓࢝ࢽࢬ࣒ࡘ࠸࡚, ࢘ࢬࣛࢆࣔࢹࣝ ࡋ࡚ᬬ⇕ࡼࡿᙳ㡪ࢆෆศἪᏛⓗゎᯒࡋࡓ⤖ᯝ, ༸㯤ࡢࢥࣝࢳࢥࢫࢸࣟࣥࡢ✚ࡀቑ ຍࡍࡿࡇ, ᬬ⇕ฎ⌮ࡀᑠ⭠㸪⫢⮚ࡢ㞀ᐖࢆ㉳ࡇࡋࡓ⤖ᯝ㸪⬡㉁௦ㅰᙳ㡪ࢆ࠼, ༸ᕢ ࡢᶵ⬟, 㯤Ⰽ༸⬊ࡢῶᑡ, 㔜㔞ῶᑡ, ༸㔜㔞, ึ⏕㞮య㔜ࡢῶᑡ࡞ࢆᘬࡁ㉳ࡇࡋࡓࡇ 㸪⭈ᛶ⭢࡛ࢫࢸࣟࢻ⏕ᡂ㓝⣲ࡢⓎ⌧ㄪ⠇SCE ࡀ␗࡞ࡿస⏝ࢆ♧ࡍࡇࡀ᫂ࡽ ࡞ࡗࡓࠋ
ᑂ ᰝ ⤖ ᯝ ࡢ せ ᪨ ᮏ◊✲࡛ࡣ㸪ᬬ⇕ࢫࢺࣞࢫࡼࡗ࡚ᐙ⚺ࡢ⦾Ṫ㞀ᐖࡀ㉳ࡁࡿ࣓࢝ࢽࢬ࣒ࡘ࠸࡚, ࢘ࢬ ࣛࢆࣔࢹࣝࡋ࡚⏝࠸㸪ᬬ⇕ࡼࡿ༸⬊Ⓨ⫱ᢚไࢆෆศἪᏛⓗゎᯒࡋࡓࠋ ࡲࡎ, ᡂ⇍㞤࢘ࢬࣛ 10 ᪥㛫㐃⥆ࡋ࡚㧗 ᭀ㟢ࡋ㸪༸ࡢ㉁㛵ࡍࡿᙳ㡪ࢆゎᯒࡋ ࡓࠋࡑࡢ⤖ᯝ㸪ᬬ⇕ฎ⌮⩌࡛ࡣ༸㔜㔞ࡀ᪥ẖᚎࠎῶᑡࡋ㸪᭱ᚋࡢ2 ᪥㛫࡛ࡣ᭷ពῶ ᑡࢆ♧ࡋࡓࠋ⏘༸⋡ࡣ⩌࠾࠸࡚᭷ពᕪࡀㄆࡵࡽࢀ࡞ࡗࡓࡶࡢࡢ㸪ᬬ⇕ฎ⌮⩌࡛ࡣ༸ ᕢ㔜㔞ཬࡧ༸⟶㔜㔞ࡀ㍍㔞࡛࠶ࡾ㸪㯤Ⰽ༸⬊ࡢᩘ༸ᕢ㔜㔞ࡀῶᑡࡋࡓࠋ⾑ᾮ୰ࢥࣝࢳࢥ ࢫࢸࣟࣥ⃰ᗘࡀ㸪ᬬ⇕ฎ⌮ࡼࡗ࡚᭷ពቑຍࡋ, ༸㯤୰⃰ᗘࡀ㯤Ⰽ༸⬊㸦F1, F2, F3㸧࡛ ᭷ពቑຍࡋࡓࠋᬬ⇕ฎ⌮ࡼࡾ⭈࠾࠸࡚ࢫࢸࣟࢻྜᡂ㓝⣲ࡢ㑇ఏᏊⓎ⌧ࡀቑຍ ࡋ㸪ࡑࡢ⤖ᯝ༸㯤ࡢࢥࣝࢳࢥࢫࢸࣟࣥࡢ✚ࡀቑຍࡍࡿࡇࡀุ᫂ࡋࡓࠋ ḟ㸪ᬬ⇕ฎ⌮ᙳ㡪ࡢ࣓࢝ࢽࢬ࣒ࢆ᫂ࡽࡍࡿࡓࡵ㸪⾑Ύ୰௦ㅰ≀ྵ᭷㔞ࢆゎᯒࡋ ࡓࠋ⾑Ύ୰165 ࡢ௦ㅰ≀ࡀ᳨ฟࡉࢀ㸪ࡑࡢෆࡢ 7 ࡘࡘ࠸࡚ᑐ↷⩌ᬬ⇕ฎ⌮⩌ࡢ㛫࡛
᭷ព࡞ᕪࡀㄆࡵࡽࢀ, butanoate, propanoate,࠾ࡼࡧ pyrimidine ࡢ௦ㅰ㸪ࢣࢺࣥయࡢྜᡂศ
ゎ㸪cyanoamino acid ࡢ௦ㅰᙳ㡪ࢆ࠼࡚࠸ࡿࡇࡀ♧ࡉࢀࡓࠋࡉࡽ㸪ᑠ⭠⫢⮚ࢆ⤌ ⧊Ꮫⓗゎᯒࡋࡓ⤖ᯝ㸪ᬬ⇕ฎ⌮ࡀ༑ᣦ⭠㸪✵⭠, ᅇ⭠ࡢ⤧ẟࡢ㧗ࡉࢆῶᑡࡉࡏࡿࡇ ࢆ᫂ࡽࡋࡓࠋ௨ୖࡢ⤖ᯝࡣ㸪ᬬ⇕ฎ⌮ࡀᑠ⭠㸪⫢⮚ࡢ㞀ᐖࢆ㉳ࡇࡋࡓ⤖ᯝ㸪⬡㉁௦ㅰ ᙳ㡪ࢆ࠼ࡓࡇࢆ♧၀ࡋࡓࠋᬬ⇕ୗ࡛⫢⮚ࢆಖㆤࡍࡿࡇࡼࡾ㸪ኟᏘࡢ㧗 ᑐᢠ ࡛ࡁࡿྍ⬟ᛶࢆ♧ࡋࡓࠋ ࡉࡽ㛗࠸ᮇ㛫㸪20 ᪥㛫㸪ࡢᙳ㡪ࡘ࠸࡚㸪ᡂ⇍ࡋࡓ㞤࢘ࢬࣛࢆ⏝࠸㸪⏕Ṫᶵ⬟ࡢ ᙳ㡪ࢆゎᯒࡋࡓࠋᬬ⇕ฎ⌮ࡼࡗ࡚㸪༸ᕢ㸪༸⟶㔜㔞ࡶ᭷ពῶᑡࡋ㸪㯤Ⰽ༸⬊ᩘ㸪 㔜㔞ඹῶᑡࡋ, ༸㔜㔞ࡀῶᑡࡋࡓࠋཷ⢭⋡ࡣኚࡀ࡞ࡗࡓࡀ, ᬬ⇕ฎ⌮⩌࡛, ึ⏕ 㞮ࡢయ㔜ࡀ᭷ពῶᑡࡋࡓࠋ⫢⮚ࡢ⤌⧊ゎᯒࡢ⤖ᯝࡣᬬ⇕ฎ⌮ࡀ⫢⮚ࡢ⬡㉁௦ㅰ␗ᖖࢆ㉳ ࡇࡍࡇࢆ♧ࡋࡓࠋ⾑ᾮ୰࢚ࢫࢺࣛࢪ࣮࢜ࣝ-17șࡢ⫢⮚࡛ࡢ௦ㅰࡶയᐖࡉࢀ, ༸ᕢࡢᶵ⬟ , 㯤Ⰽ༸⬊ࡢῶᑡ, 㔜㔞ῶᑡ, ༸㔜㔞, ึ⏕㞮య㔜ࡢῶᑡ࡞ࢆᘬࡁ㉳ࡇࡋࡓ⪃࠼ࡽ ࢀࡓࠋ ᬬ⇕ฎ⌮ᑐࡍࡿࢧࢺ࢘࢟ࣅ࢚࢟ࢫ㸦SCE㸧ࡢຠᯝࢆ, 㞤࢘ࢬࣛࢆ⏝࠸᳨࡚ウࡋࡓࠋヨ ᩱῧຍࡋࡓ⤖ᯝ, ᬬ⇕ฎ⌮ࡼࡿ༸㔜㔞ࡢῶᑡࢆࢃࡎ⦆ࡋࡓࠋࡋࡋ࡞ࡀࡽ SCE ᦤྲྀࡢᬬ⇕ฎ⌮ᑐࡍࡿຠᯝࡣ㝈ᐃⓗ࡛࠶ࡗࡓࠋ 㞝࢘ࢬࣛࢆ⏝࠸࡚SCE ᦤྲྀࡢຠᯝࢆ᳨ウࡍࡿࡓࡵ㸪㞝࢘ࢬࣛࡢᛶ⾜ື⢭ᕢᶵ⬟ ࠼ࡿస⏝ࢆ᳨ウࡋࡓ⤖ᯝ, ᑿ⾜ື㸪⾑୰ࢸࢫࢺࢫࢸࣟࣥ⃰ᗘࡀῶᑡࡋࡓࠋSCE ࡣ┤᥋⢭ ᕢࡢ㛫㉁⣽⬊స⏝ࡋ, 3șHSD ࡢⓎ⌧ࢆᢚไࡋ࡚⢭ᕢᶵ⬟ࢆᢚไࡍࡿ⪃࠼ࡽࢀࡓࠋ ୖグࡢࡼ࠺⏦ㄳ⪅ࡣ, ᬬ⇕ࢫࢺࣞࢫࡼࡗ࡚ᐙ⚺ࡢ⦾Ṫ㞀ᐖࡀ㉳ࡁࡿ࣓࢝ࢽࢬ࣒ࡘ ࠸࡚, ࢘ࢬࣛࢆࣔࢹࣝࡋ࡚ᬬ⇕ࡼࡿ༸⬊Ⓨ⫱ᢚไࢆෆศἪᏛⓗゎᯒࡋ, ༸㯤ࡢࢥ ࣝࢳࢥࢫࢸࣟࣥࡢ✚ࡀቑຍࡍࡿࡇ, ᬬ⇕ฎ⌮ࡀᑠ⭠㸪⫢⮚ࡢ㞀ᐖࢆ㉳ࡇࡋࡓ⤖ᯝ㸪⬡ ㉁௦ㅰᙳ㡪ࢆ࠼, ༸ᕢࡢᶵ⬟, 㯤Ⰽ༸⬊ࡢῶᑡ, 㔜㔞ῶᑡ, ༸㔜㔞, ึ⏕㞮య㔜ࡢ ῶᑡ࡞ࢆᘬࡁ㉳ࡇࡋࡓࡇࢆ♧၀ࡋ, 㠀ᖖព⩏ࡀ࠶ࡿࡶࡢㄆࡵࡿࠋ ௨ୖࡘ࠸࡚, ᑂᰝጤဨဨ୍⮴࡛ᮏㄽᩥࡀᒱ㜧ᏛᏛ㝔㐃ྜ⋇་Ꮫ◊✲⛉ࡢᏛㄽ ᩥࡋ࡚ศ౯್ࡀ࠶ࡿㄆࡵࡓࠋ ᇶ♏࡞ࡿᏛ⾡ㄽᩥ 㸯㸧㢟 ┠㸸+HDWFKDOOHQJHLQIOXHQFHVVHUXPPHWDEROLWHVFRQFHQWUDWLRQVDQG OLYHUOLSLGPHWDEROLVPLQ-DSDQHVHTXDLO&RWXUQL[MDSRQLFD
ⴭ ⪅ ྡ㸸3868VXGD.1DJDRND.DQG:DWDQDEH* Ꮫ⾡㞧ㄅྡ㸸7KH-RXUQDORI9HWHULQDU\0HGLFDO6FLHQFH ᕳ࣭ྕ࣭㡫࣭Ⓨ⾜ᖺ㸸,Q3UHVV ᪤Ⓨ⾲Ꮫ⾡ㄽᩥ 㸯㸧㢟 ┠㸸7HUWEXW\OK\GURTXLQRQHDWWHQXDWHVVFURWDOKHDWLQGXFHGGDPDJH E\UHJXODWLQJ1UIDQWLR[LGDQWV\VWHPLQWKHPRXVHWHVWLV ⴭ ⪅ ྡ㸸<DQVHQ/<XQ&)HL:6KDR[LD3<RQJKXL=DQG &KXQ0HL/ Ꮫ⾡㞧ㄅྡ㸸*HQHUDODQG&RPSDUDWLYH(QGRFULQRORJ\ ᕳ࣭ྕ࣭㡫࣭Ⓨ⾜ᖺ㸸 㸰㸧㢟 ┠㸸6XSSOHPHQWDOGLHWDU\SK\WRVWHULQSURWHFWVDJDLQVW QLWURSKHQROLQGXFHGR[LGDWLYHVWUHVVDQGDSRSWRVLVLQUDW WHVWHV ⴭ ⪅ ྡ㸸<RQJKXL=0HL\DQ6;LDROL56KDR[LD3<DQVHQ/ DQG&KXQ0HL/ Ꮫ⾡㞧ㄅྡ㸸7R[LFRORJ\5HSRUWV ᕳ࣭ྕ࣭㡫࣭Ⓨ⾜ᖺ㸸 㸱㸧㢟 ┠㸸([SUHVVLRQRIQHUYHJURZWKIDFWRU1*)DQGLWVUHFHSWRUV7UN$ DQGSLQWKHUHSURGXFWLYHRUJDQVRIOD\LQJKHQV ⴭ ⪅ ྡ㸸6KDR[LD3&KDQJZHL4=KL/<DQVHQ/DQG&KXQPHL/ Ꮫ⾡㞧ㄅྡ㸸%UD]LOLDQ-RXUQDORI3RXOWU\6FLHQFH ᕳ࣭ྕ࣭㡫࣭Ⓨ⾜ᖺ㸸