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(1)Kekkaku Vol. 93, No. 4, 2018. 001. 285. K¨à¯¿Gb`ç¢E in vitro Bì 8P. 002. ßøCo¯¿3)E.3P superoxide dis! mutase A&sodA'm+À\%YXi\iZF ]_à. , \1,2'!,. ä9 ^À1'!eæ îg2'!W, â®3'! G I²À2'!íò hÇ4'!ÔX ó2' þ¼Áeþþqn¤vLù.µ¾¡~¤Ï ! ÜþþqnÄô²¤v¹N[2'! ³eKq°.µ¾Kq3'! Æānivþqþ4' 1'. 81'!#. ¦1'. /ÐÀnvþìÕv1'! 2' W{4þKq9vI&«9vN['. K¨à¯¿G!@eYhcU%]L II @UÖF¹ (G7JE)ßøCo¯¿BG!16SrRNA m+ÀAk ÿ7FEBG3)0´:B*P4C0!NQA+P UEì6!¢sFfQEF?AfiaêL;FûïÝ FðÙEì6!?ËàFìÕS÷48"Ã=>G! =JjÓFª×BG house keeping gene B*P rpoB m+ÀC hsp65 m+ÀF\%YXi^zåS_+A Mycobacterium avium complex&MAC'0ç¢C Ë3)0±RQP4C0ü+"6/6=!lateral gene »6A+P4CS MAC ÊFèÑ@L MAC Sì transfer EMP rpoB m+ÀðI-Fu>àKÂ*5 5:=eW^F@èPFKbðÙB¥Î6=";4Bç QA.OÖ 2 m+ÀF\%YXi^zåF¡w0p ¢C»6A+P4C0K¨àCR?A+P/£ $:9Ë03)B1D+[%^Kú»8P"4F=J 26="H9!MAC 0b`ç¢Eé%6!/@Û; x$G superoxide dismutase A&sodA'm+À\%YX 8P4C S - À § H B < 6 ="Mycobacterium i\iZS±+!ßøCo¯¿FË3)E.3P] avium subsp. hominissuis&MAH'0ç¢Eé%8P _àS£26=" (ST)1'jÓ£ýMONc5Q=ß 4CGRýFú»B÷Þ5Q!¯Rý &CR1' ¯ýEM? øCo¯¿&288 'E@+A hsp65 m+À!rpoB Añ}5Q="ç¢F¹UYJZF\Vg¿SÑ8 m+À!sodA m+ÀF\%YXi^zåS±+!;Q< PCé%0]kE©Ò6="ç¢0B"&Eú»8P QFm+ÀE@+A 1ÌS½á6Ë3)S±?= C!MAC L¡~G'62õØ6="4FõØG(é ,-B!m+ÀFËp$d&M. abscessus. subsp é%6=ÅE<JNQ!ç¢F|FSÑ|8PC÷ GjËHB'SE6="2'rpoB m+À!hsp65 m+ 1D/?="ç¢Eé%6=¯¿G THP!1 ^è ÀFBlD?=ËC3)5Q=E@+A£2 YhcU%]EM2nÚ5Q="4QNF¡w/N!K S±?="(¡w) ¸Í3)BG M.abscessus 0 67.0%# ¨à¯¿Gb`ç¢E CR1 L\Vh0_iaYÉ M.fortuitum 0 14.6%#M.chelonae 0 10.1%#;Fû0 Sy6Aì8P4C0R/?="b`ç¢G¢fB 8.3% SëJ="rpoB m+ÀC hsp65 m+ÀBË0p CR1 Sy6AHãMLlMSQH-!;QNSìV ERPöLmöErTB+P4C0!NQA+P" $6D/?=FG 8.7% B*?="rpoB m+ÀC hsp65 m+ÀBp$6D/?=F,> rpoB m+ÀF\% EK//RN9Ã=>05=!¥G!K¨à¯¿0 YXi^zåE.+A M. fortuitum C M. houstonense ìV°Sa_6A?ËàìS÷46A+Pu> DO0´:B*?=Zg%dC!hsp65 m+ÀF\%Y àSÈ¶6A+P" Xi^zåE.+A M. septicum CtËFDO0 ´:B*?=Zg%dB sodA m+À\%YXi^z åEMPDO0u>B*?=" (¡l) 3 @Fm+ÀF+ 9QFðI²R:BKË3)p$dG 90% í¬Cþ 1D·S<JD/?="6E M. fortuitum L M. septi! cum S3)8PºEG sod A m+À\%YXi^Fz å0]_CDPu>à0*P".

(2) Ä£ N 93 ª N 4 Ù 2018 r 4 Æ. 286. 003. Ó¼srjn LL!37 D MAC ¼CK7PÓ ¼§6C=->. 004. r~kZw^dCMP&HãL6'Ä£)! .MF Mycobacterium avium complex )p ûDë|¤¥aā9. é 1'!g +1,2'!³T £3'! &= Ëo4'!\O= ]ü5'!ª Ûā2'! gn U6'!Ce kÖ7'!c '8'!-ô 'S1'. 4Ð /I¬1'!. Õè2'!´b »3'! d zmë1'!( z1'!v: ð3'! Ce kÖ2'!Á\ ]11,4'. Íì¥M¥Îµ¯$÷$V}|]%n¥1'! ½²Y¹ò«eh%2'! -¦M¥Îµ¯$¬>)n3'! Ý¥|±ÔfÍ|4'! ÁM¥¥¬>5Ñeh%5'! =¦eh%6'! þï|7'! fM¥8'. Ä£Ä£É¶$8JÑ1'! Ä£þï|Îµ¯n2'! Ä£þï|Îµ¯¢3'! Ä£Ä£É¶$4'. *a+Ä£)&Ä£'.MF Mycobacterium avium complex&MAC')D¬>B,C.->!pû0À7 5QP"pûD|¤¥aBj[C=->!4QH?C H2DÜ0B5Q>-P"6/6!¬>B,SÔW7 *tÂ$ a+p m Ó ¼ s r j n ? , P cathelicidins Pho^óC6>EG@TAÜ5Q>-B-" &LL!37'Exâ¼CK7PÓ¼§6LÓ¼@DE É¶C.->!HãL6Ä£.MFu MAC )C.3P å 0 Ü 5 Q > - P 0 Mycobacterium avium ÷ð¦Lÿøho^óS{a@6;¥¦D complex&MAC'¼CK6>E~?,P"Þ MAC ¡wSí6!r~kZw^dā9CM<>!¬>B, ¼CK6>DÈhS.4B<;" *+u MAC )«õ ?wÌ0F6>-Pho^óSQæ6;" *+ù ¡D 22 ¨&M.avium 12 ¨!M.intracellulare 10 ¨'S "<%6;&Hã L 6'Ä £&5 ©'. M F u MAC -Ó¼@ LL!37 D°ö«SBIÚR8; 96 Ãr )&4 ©'D¬>B,Dt|v{ÏòzqW÷ }%mSå7"LL! 37 DGä?D MIC D S¤q {/N!}%a%vX^~iXe^byCM<> 7P"Þ-PÓ¼E CAM#RFP#EB#SM#MFLX pûB,S<O!6;"jC!©¢ îD@9Dý D 5 ãC.->Èh7P4@@6;"*Ä+LL! 37 D?6;âDS¾6>6;"<O!6;B P l ? E @ 22 ¨ C . - > MIC E 20µg/ml * ? , O!Ó¼§6E÷-4@0ñß5Q;"6/6B0N! ,/Nho^óSZ!6>!m{rb#¤SÖ -!J^~vm_zqW%ó§9¯? aho ÞÈhSÖ<; 5 =Dã@>C.-> LL! 37 S^ 7P4@CMO B MIC D_SqJ;"*Ä+ ^ói`SÖ<;"*Ä+9Q:QD¬>B,/N 2000 *Dho^óSi`7P4@0?1;"Ä£ LL!37 D MAC ¼CK7PÓ¼§6E ÷ - 0!MAC pû©¢ îD?E plasminogen LJBADÅ® )ð¦C-PãDÓ¼§6SF¸7På0qJ ho^ó0X;6>-P4@0N/CB<;"H NQ;" ;!NADPH Z\bi%fL v!ATPase![k{bc LlWqYbBADç¼ho^ó@-<;qU `g%xCºä7Pho^óKX;6>-;"Ä£p ûâD?E{ug%xSÔW7Pho^óL{ gg%xC®HQP2āØA0X;6>-;" MAC )pûC.3P©¢ îD.MFâDC. 3PjaBho^ó¿E!Ä£C.3Pho^ó ¿@N/BàEÊNQB/<;"9Q:QD¬>j aho^óSÈæ6;Ä!MAC )C.3P©¢ îDC.->3ú¨Dho^óSÊ!74@0?1 ;" *Ä+ *DÄE!Ä£.MF MAC )pûD r~kZw^dā9CM<>!Ä£.MFu MAC )¬ >B,?jaCwÌ6>-Pëv%[%Si`7 P4@0s?,P4@Sñß7P" (y·iÉ¶ õ)b r&}(M¥ÇÒ¥×á' !³Vê &Ä£þï||¤0R' !:=&0TÉ ¶0TÉ¶$|¤'.

(3) Kekkaku Vol. 93, No. 4, 2018. 005. lu|Pi÷

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(12) ° ) 93 ) 4 Ã 2018 E 4 ±. 288. 007. fk'j2 Hµx§ Mycobacterium ul! cerans subsp. shinshuense HN]ch û. 008. °§¼µ T cell epitope |uH HLA class II °Äû. 8 »M!øn ñQ. øn ñQ1)!zt {i2)!Í ó3). Æyýè²¡ãýõºY. Æyýè²¡ãýõºY1)! ÆyUy¾;¢Uy2)! °qdZÞÔUy3). fk'j2 I!úXej[1QJZ'_aijXW. îGLù:B/TPXU]WÚèíD;TýèC. S!WHO GQS*¸MRUF/D'U+Hv@D:B õnlRG.7RUB/T" fk'j2 Hµx§I! gYî\amÎù¼Ï§HvÜC.T Mycobacte! rium ulcerans C.S!ebG1/BO 1980 EG0n³ CâNB^Å9U>"PXU]2R*x9U>§I! DNA hybridization `GQS M. ulcerans G¦NB¨/ 3!/5@2HöíGiFq/3´RU>>N!M. ulcerans subsp. shinshuense(o!M. shunshuense )D hW6RU!Rá÷ ShT! P D:B99U>"¥fï/ 8DG!ÉâHL´2RÇBLC!ebG16Tfk' j2 Ù2R*x9U>HI;KB M. shunshuense C.?>"=8C&I!M. shunshuense HA/Wß :!=H7G®WiR2G;T>NG!ShT! P 1QJ þÆQS*x9U> 26 H M. shunshuense ~ç\x÷ H]chì^WÛ?:!$HgYî\amÎù¼Ï§ DHN]chûWÁ?>" L<!ShT!P Hþ]chW¯3:!$HgYî\a mÎù¼Ï§D Average Nucleotide Identity `GQT NW18F?>D8V!99% oëHÂ/ÿ>öW×: >"ÖG!ShT! P HþBH`md\Ø^'bJ(CDS) W$HgYî\amÎù¼Ï§H CDS DN:>D 8V!96 ·3 M. shunshuense Ap5GMRU!=H. GI ESX! 1 \OP MCE `md\ØFEUµöG r;TxÒ3LUB/>"L>!M. shunshuense ~ç \x÷Hv~%«GA5=û2RI!1)M. shunshuense I M. ulcerans DpFT*=ªW¬÷ :!2) ebH 5 @H,uG16Tl'[kFý$7G 34B/TFö3×È9U>" w£>²¡Ù% e oÐ1#æg à2# } 6m2#üt !ā1 1 Æyýè²¡ã ýõºY#2 ©Ý((y s ²¡y Ê§\n. ,l5-°§G&;Tj|<GI§¼µG&;T CD4 vö T cell 3r;T"=HéÊFäD HLA r7Ò«DHWiR2G;T>N!I°èÙW &êD:> HLA class II typing JÊaj|ûWÁ /!°§¼µH T Êa epitope |uWò#:LS: >"e²¡CI! T cell epitope H HLA ½"öWiR 2G;T8DWl5D:B!ÙªGÂV:C´RUT class II haplotype ëH HLA!DRB1#DRB3#4#5#DQ allele Î[G@/B¼µ T cell epitope |uDHÿ¹Ë t¤:Wû:>" ,_`-&3GLS:>_`W}t:!HLA L¶{ WÓRD:>ûWt/B!HLA class II D peptide DHÿ¹ËtH¤9Wò#:>"8HûCI!HLA class II D¼µ peptide JDWpÄ`md\ØD:B KwÊa÷GSkL¶:!ÊaSkH HLA L¶{W flowcytometer C3{;T8DGQS!HLA D pep! tide DHÿ¹Ët¤:WT;T" ,° $ÀÌ-I°èÙªGÂV:GCNRU> HLA!DR!DQ haplotype 3^'b;T HLA!DR(14 Ü) !HLA! DQ(7 Ü)G@/B!ÚuF T cell epi! tope 9 |uDHÿ¹ËtH¤9Wò#:>"8H° W G HLA!DR!DQ haplotype G1/B!T cell epi! tope W¤54×:0T HLA! DR#DQ XjkWò#: >"CFP!10 H C +¨c|uIZôH HLA! DQ allele Î[G¤54×9UT8D2R!%sF HLA haplotype £1H T cell epitope C.TD×È9U>"eLSCI 8ULCG?RU>° HuW^Å;T" ,w²¡Ù- Õ¿Ò(-H(ssz) !@z åÑ(;¢(ðr7).

(13) Kekkaku Vol. 93, No. 4, 2018. 009. 289. 0J :

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(17) ^_`.4 5g}R) ¡56//5Un3 N<. aI? 'v#Z¢c$%e)Nguyen Thi Le HangDo Bang TamPham Thu AnhVu Cao CuongPham Huu Thuong. 010. ^¦. c]£CqG3 ¤ V02 ] ~. §¨1©. S2¨^. /m3. Kªa«o;¬t$%!w5|{¤®1 -!`¯°a$%!9&{2 V±KkH$%!X²a}3. '+³´.)KªCqG3 Q÷}0 ā CqG3 ¤¶·D¸4N=CqG3 ¤ 12¹ º' 8´K>c]£*ºV02 qL/ ?V02 .?I 15 ¶ ¤ ;»_ ]6/gu¼.9½¥B]£ u¼. V02 ¶][e3 j· D¾I? ' ) Illumina HiSeq . Pair! end D»_]p¤ d ?P¶»_]p¤ ¤y¿¤ ¶ H37Rv .}ÀÁ/d<= ~C u¼ I9.V02 ¶ÂDÃ¬~*SNP UÄ9½¥ UÅ/ ?V02 15 ¶v V02 ¤ 11 ¶.R 5;»_] SNPs u¼/V02 .·¢X.·<= SNPs*V02 e[~ eZ/ ? ' āÆÇ)○V02 ¶ÂD»_]u¼É 15 ¶+ 2 F!~÷.K>u 2 R.}F= ?V02 ¶8 3 ¶+|_Ê3¶DVy :9=< 3 ¶+|_G3Ë]~ÌÍD uB SNPs s|_G3 V02 12 ¶+ 9:}Fy ?|_Ê3¶ºÎ 12 ¶+N< . 4 Á.}F= ?V02 ¶ 15 ¶Â SNPs + 51 F!DVV02 ¶+¢X9ÏF<]s/ sÐÑN=}0UÅF<Ò = 4Ó. 93 ÷I¤¶DVÆÔ<= ?○V02 ¶ B]£]u¼ÉV02 ¶+ 113 F! V02 e[vcÕ[~ Öy5 ?V02 e [vcÕ[~V].R 5_ØÙ K[ UniProtKB .5Ún UÄ ā53 .R. 5Ún:<F.y ?53 8 ¤=+ .ËI]: 16 V8 4 :zÛ3 .ËIÚn ÷/5 ?4 zÛ3Ë] 8 pknI ] · : ¤ = + negative regulation Ún Öy54pknI ]~. Ø3*ID ¤=+=rgÜÝg D ¤=+=r0YÞ.FI hy! pervirlence p:¾N=5 ?9=< āF<V 02 e[~8 pknI ]~: V02 ¶ 93.ß/5 Un3:VÆÔ<= ?.

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