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We tried to find out the kinase which phosphorylated the cdc2 kinase and inhibited the activity

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新しいサイクリン依存性キナーゼ(cdk)による細胞 周期の制御機構の解析

著者 安田 秀世

著者別表示 Yasuda Hideyo

雑誌名 平成5(1993)年度 科学研究費補助金 一般研究(C)  研究成果報告書概要

巻 1992   1993

ページ 2p.

発行年 1995‑03‑26

URL http://doi.org/10.24517/00066900

Creative Commons : 表示 ‑ 非営利 ‑ 改変禁止 http://creativecommons.org/licenses/by‑nc‑nd/3.0/deed.ja

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1993 Fiscal Year Final Research Report Summary

Mechanism of Regulation of Cdks's Activity

Research Project

Project/Area Number

04833008

Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation Type

Single-year Grants

Research Field

分⼦細胞⽣物学

Research Institution

Kanazawa University

Principal Investigator

YASUDA Hideyo Kanazawa University, Faculty of Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 (40111554)

Project Period (FY)

1992 – 1993

Keywords

cdk / cyclin / cdc2 kinase / phosphorylation / cell cycle

Research Abstract

The activity of cdc2 kinase is regulated by the phosphorylation and dephosphorylation of its own molecule. The phosphorylation of Thr-14 and/or Tyr-15 inhibits the kinase activity, whereas the phosphorylation of Thr-161 is necessary for the activity of this kinase. We tried to find out the kinase which phosphorylated the cdc2 kinase and inhibited the activity. Human weel kinase had such kind of activity. The kinase could phosphorylate Try-15 of the cdc2 molecule but not do Thr-14. The kinase which could phosphorylate Thr-14 now to be elucidated. The enzyme which phosphorylates this Trh-161 is named CAK(cyclin dependent kinase activating kinase). Recent work revealed Xenopus laevis p40MO15 kinase could phosphorylate this residue and activate the kinase. The DNA sequence of this kinase is homologus to that of R-2 protein which was isolated from rice cells as one of the cdks. We tried to clarify whether this R-2 protein has kinase activity to activate the cdc2 kinase. We found the R-2 kinase, which was produced in E.coli, could activate the HeLa cdc2 kinase in the presence of cyclin B.Also we isolated the mouse homologue of p40MO15. These kinases had NXTALRE sequence in stead of PSTAIRE of cdc2 kinase.

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(3)

Published: 1995-03-26

Research Products

(11 results)

All Other All Publications (11 results)

URL: https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-04833008/048330081993kenkyu_seika_hokoku_

[Publications] Honda,R.: "Dephosphorylation of human p34^<cdc2> Kinase on both Thr-14 and Tyr-15 by human cdc25B phosphatase" FEBS

Lett.318. 331-334 (1993) 

[Publications] Kitagawa,M.: "cdc2-like Kinase is associated with the retinoblastowa protein." Oncogene. 7. 1067-1074 (1993)  [Publications] Kitagawa,M.: "Butyrolactone I,aselective inhibitor of cdk2 and cdc2 Kinase" Oncogene. 8. 2425-2432 (1993)  [Publications] Yasuda,H.: "Cyclin dependent Kinase(cdk2)in the murine cdc2 Kinase ts mutant" Somatic Cell and Mol.Genet.18. 403-408 (1992) 

[Publications] Kitagawa, M., Okabe, T., Ogino, H., Matsumoto, H., Suzuki-Takahashi, I., Kokubo, T., Higashi, H., Saitoh, S., Taya, Y., Yasuda, H., Ohba, Y., Nishimura, S., Tanaka, N., and Okumura, A.: "Butyrolactone I, a selective inhibitor of cdk2 and cdc2 kinase." Oncogene. 8. 2425-2432 (1993) 

[Publications] Kusubata, M., Matsuoka, Y., Tsujimura, K., Ito, H., Ando, M., Yasuda, H., Kamijo, M., Ohba, Y., Okumura, E., Kishomoto, T., and Inagaki, M.: "Cdc2 kinase phosphorylation of desmin at three serine/threonine residues in the amino-terminal head domain."

Biochem.Biophys.Res.Commun.190. 927-934 (1993)

[Publications] Honda, R., Ohba, Y., A.Nagata, Okayama, H., and Yasuda, H.: "Dephosphorylation of human p34^<cdc2> kinase on both Trh-14 and

Tyr-15 by human cdc25 B phosphatase." FEBS Lett.318. 331-334 (1993) 

[Publications] Kitagawa, M., Saitoh, S., Ogino, H., Okabe, T., Matsumoto, H., Okumura, A., Tamai, K., Ohba, Y., Yasuda, H., Nishimura, S., and Taya, Y.: "Cdc2-like kinase is associated with the retinoblastoma protein." Oncogene. 7. 1067-1074 (1993) 

[Publications] Yamashita, M., Fukuda, S., Yoshikuni, M., Bulet, P., Hirai, T., Yamaguchi, A., Yasuda, H., Ohba, Y.and Nagahama, Y.: "M-phase-specific histone H1 kinase from fish oocytes : its purification, components and biochemical properties." Eurp.J.Biochem.205. 537-543 (1992) 

[Publications] Honda, R., Ohba, Y.and Yasuda, H.: "The cell cycle regulator, human p50^<weel>, is a tyrosine kinase and not a serine/threonine

kinase." Biochem.Biophys.Res.Commun.186. 1333-1338 (1992) 

[Publications] Yasuda, H., Nakata, T., Kamijo, M., Honda, R., Nakamura, M., Ninomiya-Tsuji, J., Yamashita, M., Nagahama, Y.and Ohba, Y.: "Cyclin dependent kinase (cdk2) in the murine cdc2 kinase ts mutant." Somaitc Cell and Mol.Genet.18. 403-408 (1992) 

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