• 検索結果がありません。

CD206+ tumor-associated macrophages promote proliferation and invasion in oral squamous cell carcinoma via EGF production

N/A
N/A
Protected

Academic year: 2021

シェア "CD206+ tumor-associated macrophages promote proliferation and invasion in oral squamous cell carcinoma via EGF production"

Copied!
2
0
0

読み込み中.... (全文を見る)

全文

(1)

九州大学学術情報リポジトリ

Kyushu University Institutional Repository

CD206+ tumor-associated macrophages promote proliferation and invasion in oral squamous cell carcinoma via EGF production

エーエスエム, ラフィウル, ハクエ

https://doi.org/10.15017/4060078

出版情報:九州大学, 2019, 博士(歯学), 課程博士 バージョン:

権利関係:© The Author(s) 2019 This article is licensed under a Creative Commons Attribution 4.0 International License

(2)

(様式6-2)

氏 名 A S M Rafiul Haque

論 文 名 CD206+ tumor-associated macrophages promote proliferation and invasion in oral squamous cell carcinoma via EGF production

(CD206陽性腫瘍随伴性マクロファージはEGF産生を介して口腔扁平 上皮癌の増殖と浸潤に関与する)

論文調査委員 主 査 九州大学 教授 森 悦秀 副 査 九州大学 教授 自見 英治郎 副 査 九州大学 准教授 熱田 生

論 文 審 査 の 結 果 の 要 旨

Tumor-associated macrophages (TAMs) promote tumor progression and inhibit anti-tumor immune response by producing various mediators, such as IL-6, IL-8, IL-10, EGF and VEGF, and preferentially express CD163, CD204 and CD206. However, the role of these TAM subsets in oral squamous cell carcinoma (OSCC) remains unclear. Thus, the candidate examined the expression and role of TAM subsets in OSCC, especially in cancer cell proliferation.

Forty-four patients with OSCC were analyzed for the expression of TAM markers and EGF, one of the proliferation potency cytokines by immunohistochemistry. EGF production of TAM subsets isolated from the OSCC patients was assessed by flow cytometry. Effects of conditioned medium from TAM subsets on the proliferation of OSCC cells were also examined.

CD163+ cells were detected diffusely all over the tumor and connective tissue area, while CD204+ and CD206+ cells were mainly detected in/around the tumors. Flow cytometric analysis revealed that CD206+ TAMs strongly produced EGF compared with CD163+ and/or CD204+ TAMs. Proliferation and invasion of OSCC cells cultured with conditioned medium of CD206+ TAMs were strongly enhanced and inhibited by anti-EGFR. The number of CD206+ TAMs positively correlated with the clinical stage and T classification. In addition, the ROC curve shows higher sensitivity and specificity of CD206+ expression for adverse prognosis.

The results revealed differences in localization and EGF production among these TAM subsets.

CD206+ TAM was suggested to play a key role in the proliferation and invasion of OSCC via EGF production.

They were considered new findings that suggest a possibility to control the OSCC’s growth and invasion. Thus, the present study was deemed to be worth awarded a degree of PhD (Dental Science).

参照

関連したドキュメント

In this study, we investigated whether tranilast inhibits the effects of TGF-b-induced EMT in HPMCs by inhibiting the TGF-b/Smad pathway, and whether fibrosis can be attenuated in

PD-L1 expression was upregulated in macrophages and dendritic cells (DCs) in high-grade invasive human OSCC tissues or co-cultured with mesenchymal-phenotype OSCC cells in

In this study, PD-L1 protein extracted from the cell membrane was found to be downregulated in OSC-20 cells compared with OSC-19 cells, despite a higher PD-L1 expression in the

(24) Similarly, T26 inhibited both Pim-3 kinase activity in a cell-free system and in vitro cell proliferation of human pancreatic cancer cell lines at micromo- lar

NELL1 (a) and NELL2 (b) mRNA expression levels in renal cell carcinoma cell lines OS-RC-2, VMRC-RCW, and TUHR14TKB and control HEK293T cells were analyzed using quantitative

Western blotting: The increased expression of EZH2 in carcinoma cells (TGBC2TKB) was reduced by SAHA (1.0 lM for 48 h) and EZH2 siRNA treatment, whereas EZH2 was not expressed in

Accordingly, our results showed that kahweol acetate and cafestol inhibited the proliferation and migration of both ACHN and Caki-1 cells, with their synergistic effects apparent

In immunostaining of cytokeratin using monoclonal antibodies, the gold particles were scattered in the cytoplasm of the hepatocytes and biliary epithelial cells