田 t s f d a d i e t肋" ( c o n m l ) a n d p a i ﾄ f b d g m u p s ､ T i l m o r ‑ b e a r i n g 恥ｗｅ配 i m p l a n t e d w i t h 5 × l O 6 S L C c e l l s a n d m a i n t a i n e d f b r 2 1 d a y s ､ S e m m

(1)

学術論文

岩手県立大学盛岡短期大学部研究論集第１５号２０１３年３月

BullelinofMol･iokaJuniorCollegelwatePre従cturalUniversity,No.l5

1‑6MarchZOl3

E従ctsofnmorBearingonSerumandLiverLipidLevelsinSatoLungCarcinomａ

（SLC)‑ImplantedRats

佐藤肺癌移植ﾗｯﾄにおける血青および肝臓脂質レベルに対する担癌の影響

MasashiKAWALSAKrl 川崎雅志

T11ee碇ctsoftumor造beaIingonserumandliverlipidlevelsweneinvestigatedinSatolLmgca1℃inoma(SLC｝implamedl3tsb6/compalinglonomlal

田 t s f d a d i e t肋" ( c o n m l ) a n d p a i ﾄ f b d g m u p s ､ T i l m o r ‑ b e a r i n g 恥ｗｅ配 i m p l a n t e d w i t h 5 × l O 6 S L C c e l l s a n d m a i n t a i n e d f b r 2 1 d a y s ､ S e m m

totalcholeste1℃l,andespeciallyhigh‑densiwlilpopmtein(HDL)‑cholesterolandesteriiiedcholesterolconcemanons,wercsiglificanllydecreased

f b l l o w i n g t u m o r i m p l a n t a t i o n a s c o m p a l 1 e d t o t h e c o n t r o l g r o u p ､ S e r u m H D L ‑ c h o l e s t e r o l a n d e s t e l f i f i e d c h o l e s t e r o l c o n c e n n a t i o n s i n t h c p a i ﾄ舵 d g r o u p w e I E a I s o s i g l i f i c a n t l y d e c 唾 a s e d a s c o m p a I E d t o t h e c o n t r o l g D u p ､ T h e s e m e s u l t s s u g g e s t t h a t i n S L C ‑ i m p l a n t e d I a t s , s e m m c h o l e s t e m l

conccntmtionsundeIgochange,resultinginabnomlallipidmetabolism,andthattheseserumcholesterolconcentmnon‑IEductiveactionsmightbe due,atleastinpaIt,tolhedecIcaseiI1theibodintakeaccompaniedbythetumoILbealingslaleinaddiljontothetumo雁bearinge雌ctitself

K匂ﾉ｡､h:cα雁ersα" 航/jip/q('1,〃(ﾉﾉ迄bBf"･"１９癌，血清および肝臓脂質，担癌

lNTRODUCTlON

ThetumorccllhasavelydistinctivemetabolismSevemlpにvious studieshaveconsideIEdthemetabolicchangCsthattakeplaceinthe hostaftertumorimplantanonMetabolicabnomlaliticsarisingfmm malnutritionandmalignancyameseeninanimalsorPatientswithcancer

c a c h c x i a 、 V h r i o u s c a n c e I s a H b c t t h c l i p i d m e t a b o l i s m . '7 ） R a t s

subcutaneouslyimplantedwiththeasciteshepatomacelllincAHlO9A showhyperlipidemiawithanotabledec1℃aseinthehigh‑densiw lipopmtein（HDL）hHctionandanenoImousincI℃aseinthe very‑low‑densitylipOpmtein（VLDL）pluslow‑densiwlipopIutein

( L D L ) 症 l c t i o n s s , 7 ) d u I i n g g D w t h o f t h e s o l i d t u m o I : 5 ) A H 1 0 9 A i s a

hepatomaandoIiginates丘omthelivemThelivercamcsoutmaiorIipid metabolism，includingcholesterol，fltWacid，andtriglyceIide biosynlheses,aswellasVLDLproductionltisthemelblEconsidelEd thathcpatomacellsmayailもctlipidmelabolisminlhchostbodyfmd thatlhecancerstateafiもctslipidmelabolism・

Salollmgca1℃inoma(SLC）isamnsplamahlesolidpulmonaly lumoI・ｉｎｄｕｃｅｄｉｎａｍａｌｅＤｏｎｌｙｕｓｌｍｌｎｍｔｗｉｌh4‑nihDquinoline l‑oxide､SLCcellsinjectedimavenouslyhavebeenfbLuldtopmduce metastaticibcionlyinthelungowithalliniectedratsdyingof

p u l m o n a I y m e t a s t a S e S ､ 8 9 ）

ｌｎｔｈｅｐ1℃sentstudyうtoinvestigatethee舵ctsoftheSLC implantationonlipidmetabolismchangesinlipidlevelsinserumand liverwe肥examinedinSLC‑implantedrats,TheSLCisalungcancer andol･iginalEslmmthelungtheIEfb1℃,theeHbctsofthcimplanted tLlmorcellsonlipidmetabolismofSLCmaybesmallascomparedto

t h e h e p a l o m a 品 7 ) w h i c h o r i g i n a t e s 6 ' o m t h e l i v e I ･ a n d i s a c t i v e i n v a n o u s

typesoflipidmetabolismlnaddition，ｉｎｔｈｅｔｕｍｏ雁bearingstate， decmeasesinlbodintakeandbodyweightalEseenasaIEsultofa canceHnducedcachexiaTherefbre，apail湾fもedingstudywas conductcdtoinvcstigatethee舵ctsoffbodrestlictiononUlmoI達bealing imSLC‑implantcdl別ts．

ＭＡＴＥＲＩＡＬＳＡＮＤＭＥＴＨＯＤＳ

Ａﾉ7αAαﾉ7〔/ｄＩｾKs・Thisanimalexpemnentwasconducted wilhthcappmvalofthclwatePIEfectumlUniveIsityResea1℃h

EthicsCommittee・

MaleDonIyumts(1burweeksofage,JapanSLClnc.,ShizuokzL Japan）ｗｅ1℃individuallyhousedinstainlesssteelcageswithwire boUomsinanaiI迄conditioI1edlwoomwhhatemperaUlreof22±2℃,a ldativehumidilyoI､55±5％,ａｎｄａｎ８:Ｏ０ａｍ・ｔｏ８:００ｐ．ｍ．lightcycle andwelEkeptonastockpelletdietOV征;OIientalYeastCo.,1bkyo，

J a p a n ) ， f b l l o w e d b y a b a s a l d i e t c o n t a i n i n g ２０％ c a s c i n ､ ' 0 ） T 1 1 e

compositionol､lhebasaldietwasasfbllows:２０％caseｉｎ(Oriental YeastCo.)、13.2％a‑comstarch（O1ientalY1eastCo.)，39.75％

comsta１℃ｈ（OrientalYeastCo.)，１０％ｓｕｃｍｓｅ（NissinSugar ManufncturingCo.､Tbkyo,Japan),５％cellulosepowder(Oriental YeastCo.).7％soybeanoil(OrientalYeastCo.),3.5％mineIalmixtulE

( A I N 9 3 G c o m p o s i l i o n ) ' 0 ) ( O I i e n t a l Y e a s t C o . ) , １％ v i t a m i n m i x t u m e ( A l N 9 3 c o m p o s i t i o n ) ' ０ ) ( O r i e n t a l Y e a s t C o . ) , 0 . 2 5 ％ c h o l i n c b i t a l l m t c

(WtlkoPulcChcmicalIndustries,Osaka,Japan),and0.3％L‑cystine (WakoPuI･eChcmicallndustries).AfterplBliminalyibeding,thcllats

*lFoodandNuuitionMaiol;ScienceofLivingDepallment・

A b b 唾 v i a t i o n s : H D L h i g j l ‑ d e n s i W l i p o p m に i n ; L C A m l e c i t h i n ‑ c h o l e s t e m l a c y l t m n s f e m s e ; L D L , I o w ‑ d e n s i M i p O p m t e i n ; P B S ( ‑ ) , p h o s p h a t e ‑ b u 碇 I 迄

edsaline;TBARS,thiobaIbituIicacid‑IEactivesubstance;ＶＬＤＬ,vely‑low‑densiWlipoprotcin．

− １ −

田 t s f d a d i e t肋" ( c o n m l ) a n d p a i ﾄ f b d g m u p s ､ T i l m o r ‑ b e a r i n g 恥 ｗ ｅ 配 i m p l a n t e d w i t h 5 × l O 6 S L C c e l l s a n d m a i n t a i n e d f b r 2 1 d a y s ､ S e m m

学術論文

岩手県立大学盛岡短期大学部研究論集第１５号２０１３年３月

佐藤肺癌移植ﾗｯﾄにおける血青および肝臓脂質レベルに対する担癌の影響

MasashiKAWALSAKrl 川 崎 雅 志

田 t s f d a d i e t肋" ( c o n m l ) a n d p a i ﾄ f b d g m u p s ､ T i l m o r ‑ b e a r i n g 恥 ｗ ｅ 配 i m p l a n t e d w i t h 5 × l O 6 S L C c e l l s a n d m a i n t a i n e d f b r 2 1 d a y s ､ S e m m

c a c h c x i a 、 V h r i o u s c a n c e I s a H b c t t h c l i p i d m e t a b o l i s m . '7 ） R a t s

( L D L ) 症 l c t i o n s s , 7 ) d u I i n g g D w t h o f t h e s o l i d t u m o I : 5 ) A H 1 0 9 A i s a

p u l m o n a I y m e t a s t a S e S ､ 8 9 ）

t h e h e p a l o m a 品 7 ) w h i c h o r i g i n a t e s 6 ' o m t h e l i v e I ･ a n d i s a c t i v e i n v a n o u s

EthicsCommittee・

J a p a n ) ， f b l l o w e d b y a b a s a l d i e t c o n t a i n i n g ２ ０ ％ c a s c i n ､ ' 0 ） T 1 1 e

( A I N 9 3 G c o m p o s i l i o n ) ' 0 ) ( O I i e n t a l Y e a s t C o . ) , １ ％ v i t a m i n m i x t u m e ( A l N 9 3 c o m p o s i t i o n ) ' ０ ) ( O r i e n t a l Y e a s t C o . ) , 0 . 2 5 ％ c h o l i n c b i t a l l m t c

A b b 唾 v i a t i o n s : H D L h i g j l ‑ d e n s i W l i p o p m に i n ; L C A m l e c i t h i n ‑ c h o l e s t e m l a c y l t m n s f e m s e ; L D L , I o w ‑ d e n s i M i p O p m t e i n ; P B S ( ‑ ) , p h o s p h a t e ‑ b u 碇 I 迄

田 t s f d a d i e t肋" ( c o n m l ) a n d p a i ﾄ f b d g m u p s ､ T i l m o r ‑ b e a r i n g 恥ｗｅ配 i m p l a n t e d w i t h 5 × l O 6 S L C c e l l s a n d m a i n t a i n e d f b r 2 1 d a y s ､ S e m m

MasashiKAWALSAKrl 川崎雅志

田 t s f d a d i e t肋" ( c o n m l ) a n d p a i ﾄ f b d g m u p s ､ T i l m o r ‑ b e a r i n g 恥ｗｅ配 i m p l a n t e d w i t h 5 × l O 6 S L C c e l l s a n d m a i n t a i n e d f b r 2 1 d a y s ､ S e m m

J a p a n ) ， f b l l o w e d b y a b a s a l d i e t c o n t a i n i n g ２０％ c a s c i n ､ ' 0 ） T 1 1 e

( A I N 9 3 G c o m p o s i l i o n ) ' 0 ) ( O I i e n t a l Y e a s t C o . ) , １％ v i t a m i n m i x t u m e ( A l N 9 3 c o m p o s i t i o n ) ' ０ ) ( O r i e n t a l Y e a s t C o . ) , 0 . 2 5 ％ c h o l i n c b i t a l l m t c