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(1)Studies. the. on. Composed. Crystalline. Mainly. MgN払PO4･6H20. of by. Substance. Collyb='a. Produced. (Ⅰ). q,elutipes By. Sh6ji WAKITA. The which. has studied on the author has a short aerial mycelium,. yellow. brown. When. in. 1.. Mycelia. varied. myceliumァas. 5o), and After. several for. its tendency, mycelium. Then. reached the. method･1). author That. repeated. every. of Collybia. in. white. its. young. was. years. a. its. maintained. one. uelutipes,. period,. the part. and. for. two about This short. medium.. mycelium. agar-agar. the as. (Ballg.. medium. author. it bad. been,. development. mushroom. under. As. for. months.. left. of the. 17c. mycelium).. extract. mycelium,. being. preserved months･. three. the. colour. and. malt. malt-extract. every. of culturing. maturity,. eighteen. a. normal. renewed. example,. is, itx was. on. mycelium･ on. of 15 to. cool place. (Fig. 1, normal. cultured. maintained. its culture. a. there. seen. were. Fig.. in. incubated. was. weeks,mushrooms. aerial. looking. its maturity.. latter. the. development. mushroom. to. even. when. becoming. following. mycelium mineral. the. came. culture. oil, and. its. of another. the. notice the tardv.. B口ELL,s. culture part. was. of. the.

(2) S. Wakita. 二2. it. mycelium, three. ･every. After into. was. in wood, 1, (Fig. varied a. 67 ". collybia. to. be. seen. :24o after. for. about. mycelium, ium. and. returned a. the. the. repeated. triclinic. Fig.. author was. which. the. normal. the. little. culture the. mycelium,. system. 2･. became. to. old, for血ng. composed. Crystalline. when. of. substances. seen. components. have. exuded. some a. about. of. it to. the. "Collybia. In the. phosphorus,. the. in. substance. in. of magnesium. velutiPes.. to. at the. 17o. varied potass-. medium. phosphates. in Collybia. in 15. at. there. as. differences. noticed. incubated. grow. oil. incubated. were. were. was. veluti-. of mineral. distinct. they. such into out. crystalline 99 percent. detected. few. a. develop. and. cover. variations. no. were. inorganic. found. or. and. mushrooms. contr早ry, were. were. is, when. That. mycelia･. the. =Hodagi‖. on. grow. mycelium. under. There. to. it after bring out. not. but. medium,. changed. even. and It did. seen. this. preserved. mycelium,. weeks,. agar were. named. one.”. normal two. the. the. natrium. into. The. one. or. have. to. seen. before.. as. liquid. a. was. mycelium,. aerial. mushrooms. maximum-growth,. two on. on. small. mycelium).. between. 一皿aXimum-growth. when its. like. being. medium,. mycelium. remained. colour. cultured tube,. velutiPes 67,. of. ･component. was. test. a. and. var･〃. .pes. its. long. a. possessed. it. it, this. of culturing. maturity,. when. -mushrooms ･or. years. which. full. reached. malt-extract. months. four. fungus. a. the. following. on. carried. after. the it･. medium. It looked. ammonium. X9･. the.

(3) Studies. In. little. a. -phosphate,. of. calcium. fungl,. certain. Crystalline. the. on. Substance. oxalate,. Crystalline. a. gave report -the. explanation been. not. a. medium. in. cal characters. 1･ in the. Some. the. of. mycelium. uelutil'es. of being. of. substance. magnesium. a. report the. and. as. cultured. on. and. pro-. days. is found. ammonium the. On. any. such. nature. formany. myceliumcultured. But. substance. several lost its orlglnal gradually. crystalline. for. the-. acid. pho&phate. and. physical of. mechanisms. chemi-. crystalliza-. theてOther. its. with mycelium in the mycelium. grew. and. chitin. in. grew. the. crystallization. inorganic the. phosphoric. broken. were. components. there. pH. of magnesium. rose,. which. ammonium. resulted phosphate.. in. resulted. also magnesium. normal. compounds. 1nOrganic. carbohydrates. mycelium,which of. phosphoric. contrary,. other. and. for the crystallization. organic. the. on. where,. :. mycelium. varied. and. materials,. the. of. a. of. destroyed,. cell. good condition 4. Oxalic acid. dition. years. HAMADA. mellea･5). crystalline. after. will glVe. nucleic. the. out. exuded 3･ a. of. of Collybia. author. were. Ammo中a. in. oxalzLte. composed. maximum-growth. tacid increased, 2. Some of and. Especially,. oxalate.. were. which. mycelium:the. After. the. the. of. in the. tion. mainly. substance following,. the. velutipes,. noticed,2-5). in Armillaria. mycelium. varied. often. 3. (Fig. 2).. of silica. were. disc?very. the. repeatedly, In the varied. one･. varied. a. Collybia. words,. crystalline. And. in. Composed. sodium. of calcium on. made. detected. author In other. duced na. detailed has. malt-extract. ･a. or. trace. a. and. substances. TepOrted′-短軸potassium. Mainly. a. ammonium. good. con-. phosphate,. MgNH4PO4･6H20. 5･. The. existence. 1ium. and the MgNH4PO4･6H皇0.. medium. Ij 1･. Acquisitior! Mycelium. the. of. Malt-. Incubation Formation. of the. promotion. 30. (mycelial. down･. 40. mass), of. half. About. days),. Projected was. ･crystalline. the. myce-. crystallization. of. Mycelium. a. was. day. scooped substance. of the. out. after,. by. a. from. npedle･ the. surface In mycelium. of. a. when. passing, dried. mycelium, it. became. was. up.. water,. tip. of. di氏cult. older.. the and. the. in. produced. After. in the. the. were. mycelium. substance:. washed. of the. maximum-growth. substances. the. as. crystalline it. the. crystalline. increasing. the. days,. 70. about. to. After. substance､:. Lair stance. the. in. (Balling 9o).. crystalline for. (cropping). substance. the. of. in. I)etected. salts. substance 67 ∀ar.. velutipes extract. mycelium. upside. the. of magnesium. 24o.. (incubated. incubated. to. SubstarLCe. Collybia. :. amount. crystalline. Collection 闇as. led. also. ㌔:. mycelium the. moderate. Crystalline. :. Medium. a. of. the. mycelium. dried. in. crystalline. crystalline to. up. scoop. subout. a.

(4) S. Wakita. 4. Re丘nement was a. to. added glass. were. got. rid. crystals. then,. By. temperature-70. to. 2.. Properties. (1) General. it by. mycelia. the. of the. there. (30. water. added. left. that,. noted. the. crystal,. crystaⅠ. these. treaト. water. when. it turns. dull. with. the. the. with. 40cき. to. intensely. stirred. little impurlty. was. to. crystalline. they. while,. it iTluSt be. way,. 80o-is. distilled were. sticking事tO and other江aterials decanting beaker･ When the of. means. times,. Several. line substance.. after. a. Warm. :. substance. and. the. of from. dolle. were. meat. the. and. rod,. the crystalline. of. crystal-. higher. of. instantly･. substance. properties. it was (Fig･ 2), and when and brittle substance semitransparent heated became it was at 120 to 130o im in air, its surface dull. When dried same in form the one remaining･ third in volume, air, it lessened,'into about In hot water, it dark it turned dull, and as it was, became gray. and when brittle (Fig. 3),. 5 minutes, it turned boiled at loo° for about white was and. lt. being. was. soluble. NaOH,. 3･. analyzed. were. substances. analysis:. 3 percent. When. acids･. the solution together. produced. readily. Crystalline. was. following. by ammonia,. phosphorus, acid. inorganic. in. treated. boiling. was. with. for. water. neutralized. with. ammonia･. 5. ×9･. minutes･. Analysis. Generま1 was. inーhot. precipitations. Fig･. (2). a. separated :. the. general. HCI method,. little calcium from. the. solution. and. crystalline. of. the. which. brought. oxalic. acid. substance. substance. crystalline out. much. (about l% and. or. con丘rmed. magnesium･,. less)･ 0Ⅹalic as. in. the.

(5) Studies. 2 percent. in. a. 30ml.. of. solution. H2SO4. diluted. 二and had. calcium. were a. to. ｡added. H2SO4. COnC･. dissolved. for. basi丘ed with. solution. bottle and left. Another. needle-. whitish. the. crystals. the. were. crystals,. Was. instead. of. from. the. did. the. fall. 100. was. it. when. to. was. lOIo, mixed. therefore, that the recrystallized confirmed, Ultraviolet : rays About absorption spectrum. added treated. one. the. gram.. ter皿OStatt. Of. of. crystals. hour. in. with. distilled. washed. was. solution. ･oxalic acid. O･390. by. a. water,. examined. the. with. when 20mg.. rays. ultraviolet. They 125o.. lOml.. whole percent. absorpt10n. led on. percent. of. solution. of. FeSO4. in. the. with. in. Was. When were re-. temperature. pure. oxalic. acid.. was. oxalic. acid･. distilled. of. it･. substance. this. and. the. above-. crystals. the. Of. to. concenト. the. fused. were. poured. which drying. substance. After. the. was. polnt. was. to. precipitates. then. and The melting. acid,. acetic. solution. produced. heated,. water even. a. Were. was. whitish. After one. a. washed. solution. with. night,. it,. about When. preclpltateS. dark.. distilled not. and. crystals. turning. acidほed that. whole in. obtained.. yellowish to. added. ･crystallized was kept and. was. substance. the. Ⅵ㌻as. ether. of the ether. crystals. weighed. the. into. made. solution. of. result. was. like. part. part. it. as. 5. was. The. NH40H,. the. added,. it.. One. with. Composed. substance. to. water. hours･. 48. Mainly. crystalline. distilled. water,. crystalline. -mentioned. the. ether. chloride. of. H2SO｡,. rated. land. of. Substance. the. of. in the. weighing. ･appearance. It. adding. with. produced. into. SOlution. by. circulation. distilled,. Crystalline. tne. on. a. glass. 甲ater tube. was. and. it was丘1tered to 90 ml” amounted. beingcooled, quantity. of. ammonium'oxalate The result SPeCtrum･. and is shown. in丘g.4.. 4.6. ∧ヘ. l.5. ＼鞄. ′￣￣●ヽ. I.尊. 娩';a3tl?.ll.◎ 4. 宅3. E･2. ､占. 0一/`■○ ′. ○′. @. ■. O. i.EI ヰ. Lc.. ＼ゝ｡. (曙. ヽ. a.好. ヽ. ＼ヽヽ. 色7. &oO. 2(1. }Q8. 3Ql. 2I占. .押0. Z&P Fig.. 4.. Ultraviolet substance. absorption in. spectrum aqueous. of solution.. the. crystalline.

(6) S. Wakita. 5. line. Bright. little calcium,. a. sium,. Quantitative two. The. spectrum:. These. stance.. twoりしWere. by. Components. of. crystalline. substance･. the. sub･ is result. The. the. 43. 70. 41. 94. Mg. 10. 24. 10.56. Ca. 0･43. C204E2. o.78. Molar. 7. 35%. the. 38. 71 9. 91.. O･48. l. of. ratios. MgNH4PO4･6王i20. 7. 07%. Pod. o.91. in. co皿pOユentS. the Mg. 1. PO4. crystalline. substances･ 1. C204王i2. Ca. Crystalline. 1.0. 1.2. 1.1. 1.0. 1.2. Powdery. 1.0. 1.3. 1,2. 1.0. 1.2. from. Judging there. the. be. will. little. a. substance. (3) Measurment Theory. water. in the. The. general. and. the. the. of for. NHS them. and. the. itself decide. weight. H20. is. is obtained. crystals. the. to. a. of. by. the. crystalline. for. measuring or. is. treatment. same. in. water. hour. an. its decrease. ammonium. way. the. separated. ammonia the. to. so,. and tin. repeated the. means. weight. of. crystalline. water. So. an. In. these. by. measuring丘rstly. heating. by. decreased. crystal by heating･. is decomposed. salt, which heating･. crystalline. and was. apparatus. secondly specially-. experiment･ at ■110o in the. oil is, heated issunk crystals. crushed. of. llOo. to. And. constant･. bean. Fresh the. the. from. possible of. weight. thus. is inapplicable. method. remainder. arranged. however,. crystal.. is. it. samples,. to. 105. at. weighed;. ･will. ammonia. separates. heated. be. will. is reduced. weight. in. Mg(NH4)4(PO4)2. as. such. of the. component. Pod/Mg>1,. of. substance the crystalline Generally, for the purpose. :. be. it is cooled,. after. Because. main. in. water. crystal. the. exper血ents,. MgNH4PO4･. substance. practice. the. crystal,. the. of and. be. will. these. of. result. substance. crystalline. the. into. noncrystalline. Crystalline. 忘蒜言+竺竺三三竺tN王i4. of. of. methods)･. 1. a. that. general. 6. 57%. NH4. 2.. divided. were. the. Povdery. Components. Table. substance･. crystalline. and. magne--. 2. 1.. Table. the. crystalline. and. phosphorus. substances. crystalline. analyzed. 1 and. much. in. silicon. of characteristic. in Tables. shown. The. :. analysis. part. parts-a. and. found. author. trace. a. passed in. the. over. On. H2SO｡.. is measured,. Theairof. in theoil･. and. crystals the. B-A. other is the. A. thermostatt･. and. (A). hand,. weight. about :. the. (B). :. bottle. llOo･. free. ammonia the. of water. containing. from. isolated. decreased separated. weight. from. coョ,㌔ fromofthe-.

(7) Studies. The. crystals･. a. The. :. of. 20ml.,. certain. time,. and. the. from. free. CO2.. In the line. The. corresponding. oxalate. the. crystallirle. As in. the. the. crystalline. of 471･4 the. Erlenmeyer･. ,ユan. heated. bath. llOo.. at. it. to. was. pipeted. mbO･･. (43,69%). After-. wiped. by. was. 205･9. mg･,. 471･4mg,. of. By was. out. made. constant,weight.. in. entered_. aask. difference. substance. Was. was. the. oil sticking. a. MgNH4PO4.. to. to. T. off,.. NaOH(N/50). follows:. as. was. the. in. (N/50). 265･5mg･,. Water. into. oxalate. H2SO4. mainly. in. Con°.. substance. that,. so. out,. H2SO4. result. crystalline. water,. taken. on. Composed. came. inanoil. sunken. wag-. co〇1ed harld,. other. (B). (471･4mg.). theflask､was. after. Mainly. till. on. crystal. the月･ask. weighed. On. crushed. and. Substanc3. is carried. experiment. Practice. flask. Crystrlline. the. on. the. little. so. that. taking. without. and. the. way,. was. the crystal-205.9mg., was,. existence. the. the. of. calcium.. calculation. existence. the. of. of. calcium. consideration. molecular. of MgNH｡PO｡. weight. 137.4, the. was. of MgNH｡PO逢. moles. was:. sample. 265･5/137A-1･93. is, the. That. weight. to. corresponding. in. H20. the. sample-. WaS:. 18.02 As. the. weight. led. of. be. to. proved all,. (molecular. to. the. the. in. water. the. constant. of. in. water. the. crystalline. MgNH4PO4･6HりO. that. result. (g.) water)×1.93-34.78 was sample 205.9(g,), 205.9g./34.8g.-5.92. of. weight. substance, the. was. was】. after. while,. ingredients･. principal. (chief elements). (4) X-r.ay diffraction Phosphorus,. of the. ponents. in. ammonia, crystalline. following,. the. seems. oxalate,. (about. a. and. abundantly. contained. effect. the. a. upon. concluded little. calcium. The. phosphate. The. is shown. crystalline. magnesium That. being. in. the. substance. of MgNH｡PO｡･6H,20. the. Fig.. is. of. mixed. calcium. approximately. crystalline and. a. crystal. com--. mentioned. in the. solution. of little. a. of. in. size. of. it7).. crystalline much. with with. each. substance, wel･e. oxalate. does From. is.. which have. not. any. this, it will.. all. substance. of. is.. component. material. ingredient. minor. the. the. of. the. containing. MgNH｡PO4･6H20. and. other. the. synthetic. experimented. ammonium respectively･. 5.. it is found. and. As. is composed. contairled. the. except. the. case,. phosphate, is,. The. diffractions. and. grows. formula. a. crystal･. which. which this. crystal wi[h. formula. X-ray. magnesium. Such. mixed. coincide will completely diffraction of the crystalline. X-ray. result. in the. oxalate. MgNH4PO4･6H20. a. mycelium,. the. are. calcium. MgNH｡PO｡･6H20.. a･nd. coincides. lattice that. for. and. in the. substance. crystal-mixed,. mixed. constant,. always. produced. crystalline. calcium oxalate lattice formula of. acid. oXalic. substance. the. l%). The. be. magnesium,. not. with. substance. little of. that,. X-ray. the. coincides. witil. calcium. oxalate.. was. calcium. a. mixed. oxalate.. diffraction the. formula. synthetic. crystal. of. the-. ammonium. containing. much.

(8) S. S. Wakita. CaちOF2ち0. h. …[ ヨ. J1. ･11 の. J3. ￠＋〉 f3 ”. h .P ･J 吟. 貞￠ヰ} 白 =. synthetic. crystalline. ∫.0. ”. nagne8itln. amnOnium. phosphate. the. from. stlbs七ance. mycelium. ＋}. ･J a) f= ￠. 0.5. .p 【王 =. 0. 0.8. ot. 1. I__⊥+ o.5. 0.6. 0.旦. q3. 0.%. o.. a. a .log Fig.. 5.. Diffraction. the. of. crystalline intensity. (Maximum. :. by. substance 1. 00). X-rays.. TerhPer&tureS. a80l. ￠. I. lbOO. I(0.. l如○. ○. I l I l l l. 3o ■′■ヽ. 1県. 如. ㌔_′. t. l l l. 0 ･r+ ヰJ a ”. 50. L. ⅣH乍 1-. 勺 q〉. ･1-←●--1f. 6o. ●----一-----○----I----一---I.---. -●--. --J?-I---*____.. 詔￠ ” O O. 7o. A. 8e ○-●-○-○-o-●. ?○. E&0 o--●-●---･● -. I. 1oQ O. 2O. .o. 3o. lRO. ljO. IW. -●-●-4-4. IBO. .So. flo. E80. E†O. acO. &)0. Hour8. Fig･. (5). Transformations lts. Lby. Destruction. 6･. the. measured.. crystalline. substance. in. The. result. order, is. (in air),. used. for the. Crushed. 140o. beating. air:. ln. that. by. substance. by heating substance Some have experiments. crystalline. as. arrangement. ･in the crystalline 100, 110 and r88, were. the. of beat-resisting. nature same. of. the. of. and given. crystals water in. of. and Fig.. quantitative. ammonia. 6.. analysis. 480.4皿g.. Were. delivered. been. Of. made water･. heated from. at them.

(9) stて1dies. It is natural, 28. percent. the. water. of. ammonia. and. the. from. was. in. it. ･was甲Ore. was. when the. than. notable. the. the. found. that. heated,. and. about Though. in. contained. the. synfrom am-. treatment･. same. from. substance. crystalline. that, liberation phosphate. magnesium. a. easily separated 46 percent of the. after. especially. both. were. substance. be. so. residue. hours,. remainder･. ammonia. not. the. ammonium. the. in result,. and,. with. in. 210. Of. crystalline. could. case,. still remaining. the. way,. phosphate. former. the. Brie月.y speaking, was feeble mycelium. found. in. contained in this heating. magnesium. seen. been. 9. Composed. treatment. a. after. even. ammonia. as. Mainly. Substance. have. should. it by. phosphate. ℃nonia. that,. therefore,. ･separated thetic ammonium this. Crystalline. the. on. of. the. ammonia syn-. chemically. thesized. Its nature 372mg.. of heat-resisting the. and. for. glass-tubes tubes then :and. for. distilled a. centrifuged,. then. thirdly is. ′The result. given. 3.. Table. ln. washed一丘rst dried ether-and Table. in. Destruction. of. were. in water. three. upo･n. an. t也es,. H2SO4. ilo. at. of into. entered. heated were which four for hours-in. two. other. substances. crystalline. lOml･. 6･5) of. separator,. the. and. were. (pH. The. :. Water. water. centrifugal. hour. one. ln. four. l15o-two. to. autocrave,. secondly lastly and. COnC.I. every. the. and. ln. ethanol, analyzed･. 3. the. crystalline. by. substance Times. beating treatment. for. taken. (in water)*･. Residue 1. 4. 1. 56. 15%. 43. 55%. NⅢ4. 54. 17. 4.17. PO4. 77. 36. 54, 52. Mg. 88. 07. 81. 22. Ca. 0.00. 0.00. C204Ⅲ2. 0.00. 0.00. Ⅵ｢eigbt. *110′∼･115o,. compared. with. .ammonia, .ammonia four_hour -and. is, when and. even seen. were. treatment. mainly. latter. in. the. case. of the former of. changes. as. red follows. found and. a. At. red. found. about. 60 percent. heat,. percent. found. residue. after. brittle with. :. 2Mg(NH｡)4(PO皇)2-2Mg(POB)2＋8NH3＋4H20 2Mg(POB)2-Mg2P207＋P205. of of the. substance,. the. result. of Mg3(Pod)2･ tetraammonium. in. stronger. 61. acid,. phosphoric. chemi-. about. comparatively. 2PO4/3Mg-2･60. to heat:. at. residue.. The. the was. magnesium. approximating. Transformation. of. hour,. one. treatment･. four. be left with. to. for hours. the. mycelium･. was. phosphate. heated. was. the. from. substance. magnesium. the. composed. po鐘/Mg-2.66,. 9diphosphate. crystalline. ammonium. really synthesized heat-resisting. That. the. magnesium. of.

(10) 10. s. wakita. And. normal. magnesium. ammonium. phosphate. changes. fouows. as. :. 2(MgNH4PO4･6H20)-Mg2P207＋2NH3＋13H20 In consequence ment･. of this, that. recognizing the. went. tube,. minutes,. and. then. to. tube. residual. ammonia the. the. substance. method. of. The. experiment. ammonium. synthesized. is glVen. A-a,. that. Table. 4･. from. Products. by. substance. the. Synthetic. mycelium comp〇und*. MgNH4PO4･6. And and. In. ingly was. 3.. the. the. out. CaC12. added. a. passing. into. the. and. the-. in. the-. the. air. the. N/50H2SO4,. same. (A). was. contained. above. between. the. weight. the. of out. carried. was. beat. crystalline. weight. the. N. the. of heated.. water. on. phosphate. analyzed. chemically. 5.39%).*. of也e. The. substance,. H20. NⅢ4. Ash. 47. 47%. 6. 04%. 45. 38%. 47. 58. 6.70. 45. 60. 47. 70. 6. 94. 45, 36. the. and. oxygen,. crystalline. magnesium. though. the. of one. about. ash. percent. were. of. analyzed. calcium. was､`. substance.. the. prlnClpal. this. (P205 26･17%,. red. phosphorus. turned. consequence, the. ingredient. prlnClpal. ingredient. of the. the. of. crystalline. Mg2P207.. was. ash. from. substance. the. Acconト myceliu皿_. MgNH｡PO4･6H弓0. Consideration The. in. rest. in. found. H20. then. the. CaC12-. the. of. 4.. Materials. Crystalline. wasノ. absorbed. mentioned. is the. also. 30J. into. into. difference. therefore,. heated. Weight. ammonia as. weight. magnesium. in Table. the. The. adop[ed･. for. the. H2SO4.. of. same. in. 130o. to. hour.. with. poured. was. passed. was. weightofwater. then,. under-. apparatus. 100. increasing. And. the. weight. the. of. usually. and calcium-tube Result: C･. result. the. an. at. one. combined. was. by. caught. in. The. ammonia. mycelium. H20. and. 600｡. experト. heat:. red. the ammonia. of. calcium-tube. was. hand,. to. the. following. the. heated. was. calcium-tube･. the. (a). other. crystalline･. of. through. up. weight. the. of this. weight. burning. by. some. it. between. the. and. passing･. the. On. difference. the. time,. lleated:. the. experiment,. CO∃, NHS. water. at. this. from. heated. of the. measurement. In. CaCl2･. same. gradually. from. for. the. at. Measurement. ascertained. little. while,. from. did. For. to. resort. substance latter. the. as. to. came. crystalline. the ､experiment: The air free. of. arranged･. specially burning. tube. the. author,. transformation. same. A･∼ Methods. B･. the. the. author old. has. made. mycelium And in this. medium･ large amount. of. an. analysis. of Collybia. velutipes. crystalline. ammonium･. Of. which. substance. phosphorus,. the. crystalline was. he. substance. cultured has. magnesium,. on. found a. a. produced. malt-extract,. a comparatively little calcium, and..

(11) studies. of. the. the. is, it. That the of. The. even. all. the. ratio. of. In the. in. an. Tbe. the. in. the. oxalate･ and. especially little of. oxalate. that. surmised. too,. able. ammonium. an. make. author. ammonium,. or. be. would. they. As. the. veigbt. a. in the. contained. Mg3(PO4)2, that. the. same. it. was. absorbed. 70Al%. respectively. 13･13%,. 17･70%. N=4,. of. its. will. main. little. than. powdery. more. equivalent of. some contain it is 1, also. sur-. be. in. phosphorus. and. will. component. crystal-. the. of Mg(NH4)4(PO4)28)I. magnesium. ammonium,. the. in MgNH4PO4･6H20･. will. larger. some. contain. it. that. found. was. a. of. case. 69･17%. to contain. it surmised ratio. substaヮce ratio. found. of. case. in the. and. and. magnesium. of ammonium, in the respectively. was. PO皇/Mg. substance,. crystalline. to. than. the. the. considering. the. ammonium-. magnesium. MgNH4PO4･6H20･. phosphate, what. relations. the. mentioned. above. lization. PO4. and as. oxalate. calcium. contents. 72･23%. substance. crystalline. and. when. magnesium,. containing. the. 17･73%･. approximated. Mg. of. that. unmovable. solution. 16･92%･. ll･87%･. and. been. substance,. lO･86%･. were. And. mised. could. the. was. substances. crystalline. magnesium. acid. therefore,. basic. a. crystalline. variety,. one,. composed. substance･. crystalline. phosphorus line. be. would. powdery,. the. found. oxalic. long. had. recognitions. In. crystals･ in produced. substances. (2･20) in calcium. ratio were. magnesium. precipitated. into. distinctive. so. substance･. crystalline. was. the. Though calcium,. either. or. Not. in different. Seen. crystalline. in. calcium. and. substances. with. of. oxalates. the. crystalline. to. oxalate･. cooperation. groups. that. said were. acid. oxalic. crystalline. compose. two. the. analyzed. groups-One. composition･. same. 1.88, approximating. 1.80 to. to. be safely if they. can. mycelium, almost. between. found. were. as. element. has. two. eye･. naked. an. author into. them. to the. powdery. other. the. ones･. dividing. be. would. materials. different by. sub′stances. and. crystalline. these. of. of the. or. the. tl. Composed. of silicon･ each. crystal. crystalline. differences. trace. a. whether. one. and. of. looking. of. sure. same. each. besides,. and,. oxalic acid To皿ake. Mainly. Substance. Crystalline. the. on. be. would. between Many. oxalates?. of magnesium. in. phosphate. ammonium. been. have. reports. a. the. On. glVen. crystal-. ammonium. contained. solution. and. phosphate. ammonium. magnesium. oxalate.. Some. of. these. are:-. reports. of MgN=4PO4･6=20. (1) Crystallization. was. oxalic. containing. greater. seen. magnesium impure,. As on. the. the. oxalate,. ammonium. and. acid. in amount phosphate. of. precipitants than. the. ammonia8,9) of. ,. theoretical. the. ammonium. author. also magnesium. of the. phosphate. were. phosphate I. ,. ammonium. same. solution. containing. solution. itlO,ll). of. weight. reached. the. magnesium. ammonium. in a solution crystallized it12,13) in acid oxalic. containing later described, occasion. excess. In. (2). ･. in the. on. carried. especially. results. (3) Ammonium oxalate. was. the. above. as. precipitating. in. the.

(12) `12. s. wakita. containing. ･solution. Judging a. mixed. oxalic. from. its. acid.. heat-resisting. crystal containing about X-ray has diffraction also. of the. the. nature, one. percent. crystalline. substance. of calcium. indicated. The. oxalate.. that. all. the. above. produced. in. the. mycelium. be. will. result is. mentioned. correct.. That. is･ the. uelutipes a. and. was. a. crystalline. mixed. percentage. small. Inorganic long. according. to. organisms the. in In. the. the. the. important. a. (1) Methods. of. medium:. the. of barley,. rating-dish,. the. result. which. the author. That. is,. microin. not,iced. process. its. of. inorganic. com-. medium. in. the. vary. the. medium. mycelium, take. will. an. acid,. 89%. of. way. the. author. has. the. 2･. Inorganic. growth. mushroom Detailed. made. 27%. by. turned. into. Bail-. was. in. taken. was. and. electric. The of. an. evapo-. little. a. ash. furnace.. the. general. theoretical. culturing. when 50%. of. method6).. in the HENNEBERG,s value Collybia uelutipes20),was illust-. the. a. compared. 50%. of ash,. potassium. to find. come. of. largeamountof. comparatively. except. magnesium,. it. mushroom. and. HENNEBERG,s. the. with of phosphorus,. 366%. and. a. contained. of. 5･ use. contain. nourishments. for. water-bath, an. It. normal method. in lOOml.. 600ml･. of. fro皿=Chikurin‥. prepared. reference.. to. calcium･. -the malt-extract. in Table. malt-extract,. found. was. the. experiment. for. the. a. analyzed. The. table. on. was. of the is glVen. by. of 172･5mg.. in. ash. was. farm. red-heat. (2). -inorganic. in. malt-extract. dried. Result. medium･. the. of. the. that. us. components. components. our. ash. The. condensed･. the. in. cropped. of ash-by --982.5 mg. AnalysIS : This. in. of. experiment The malt-extract. 9o, pH 5･8, containing preparation of ash:. rated. that. inorganic. these. of. in. tells. by. affected. contents. cognition. Malトextract. 工nedium. much. be. would. mycelium. This. media,. mycelium if these. Even. part.. variety. ing. the. of. the. a. of. differences. in. various are. components. some. components. mycelium. Fungus. themedium14-19).. of. other,. each. through. study. therefore･. to. inorganic on. carried. ･culture. inorganic. those. of. the. of. medium that. observed. similar of. Components. the. of. quantity. were. content. -pounds. been. the. oxalate.. Inorganic. components. It has. of Collybia of MgNH4PO｡･6H20. highpercentage. a. contains. of calcium. II･ l･. substance. crystal. percentage was. a. of. in. it･. sulfulic In this. magnesium.. magnesium. small. 8%. very. of. each. Though of. suitable. those. medium. components. mycelium. of. the. the. mycelium. before. and. after. development studies18･19). have. been. carried. on. the. inorganic. components. of.

(13) studies. 5,. Table. Inorganic. Components. ･. P205. 40. 80. CaO. 4. 57. 0. 0777. 0. 087. K20. 20, 61. 0. 3504. 1. 276. Na20. 4. 35. 0. 0740. MnO. 0.06. 0. 0010. MgO. 17. 67. 0. 3004. 0, 082. Total. 98. 82. 1. 6800. 3. 364. HENNEBERG,s. ;. the. and for. Erlenmeyerjヲask. an. the After. have. bring. mushrooms,. tbe且ask,. burned. the. ash. little and. to. mycelia. in the. sun･. a. of the result. nearly. distilled. taken. part. of. th･e. of the that. were. for The. :. little ash. hour. dried as. seen. and. mycelium. the. malt-extract. in. the. cases･. fungl. to. seemed and. to血ake on. cooled were. a. roughly. seemed. taken. in the. about. their. reached. from. these. And. water･. conc･. out. They. sun･. forming. have. was,. mat. Were. 〃mycelial. H2SO｡. place. cool. separated. distilled. with. a. and. others. the. when. mycelial. dried. the. the且ask. were. above. upheaved,. then All. washed. one. was. total. the. of water,. from. out. the. incubation,. of. transferred. was. mushrooms. They. at lO5o. of ash. third. their. these. all dried. preparation. was. days. certain. develop皿ent･. their. were. dried. The. a. one. the､ fun飢When. maximum-growth, And mycelia･. (2) Result. it. of. the･. studied. 24o･. 20. to. in. 80ml･. on. at. 17. days,. with. before. of. was. 17. about. washed. the皿yCelia. were. 200ml･. author. mycelium. cultured. maximum-growth,. 12 to. the. to produce mushrooms･ enough upheaved the drying: When mat and mycelial. out. thirds. was. After. development:. the. of丘1amentous have been made-. in them. studies. and. of. development. the. no. 2･Og･,. KNO3. Ill. water. malt-extract,. mushroom. the experiment Collybia velutipes 67. of :. sampling. were. of the. components. the. on. velutipes. or. 1･Og･,. distilled. after. and few. however,. mushrooms,. 50g.,. glucose. before. mycelium. Collybia. in. 2･ Og,,. KH2PO4. 0･5g･,. MgSO4･7E20. medium: NH4H2PO4. 0.1g･,. aS. to. then,. 0. 163. 0. 0359. myceliu皿reaChed Df 15 to 17o.. they. 1. 756. 2.ll. Mushroom. two. 0. 6936. Fe203. 5.8). were. gノ1.. 0. 1331. 0. 0139. culture. from. medium*. 0. 82. (1) Method. to. gノ1.. SOS. inorganic. seen. medium･. extract. malt. 13. Composed. EENNEBERG's. 7. 83. culturing. (pH. Of the. ash. SiO2. spore. fungi. Mainly. substance. coI℃pOnentS. in. %. CaCla. the. Crystalline. the. on. then,. matりwhich, and. weighed･. crushed,. then. and. mentioned･. previously. experiment. same. mushroom,. of the. 6.. in Table. is shown as. in the that. case. of. pre-developed. In themushroom, of the. the one. wild. the. mushroom21)･. post-developed less･ much. of. percentage. mycelium. ash. Compared. with. was. found.

(14) 14. s. wakita. 6･. Table. Inorganic. components after. of. i皿before. tbe皿yCelium. of. a､nd. development.. mushroom Mycelia. Components. Mushroom Be for. Ash. development. After. development. 6. 17. %. 7. 79. SiO2 P205. 51. 50. SOS. 0.ll. Fe203. 2.45. .0.12 3. 95. CaO. 2. 78. 2. 97. 1. 53. q) U. E20. 26. 92. 22. 18. 46. 37. i+ C). Na20. 4.18. 7. 24. 2. 48. MnO. 0.08. 0.05. MgO. 9. 44. ,工] ∽. 7.61. 日 ●. g. EL. Total. In. 98.. the. mycelium. manganese. the. and. mushroom.. 0.23. in. the. with. room. twice. Periodical When. latter in. the. normal. the. medium. at. iron. mycelial. components. and. that. the result with decrease in the in. mushrooms. were. this of. mush-. the. mushroom,. auctuation. then,. which,. was. developed,. While. components. components. of. mycelium. in. cultured. little. soon. of the. components. mycelia. former,. the inorganic. and. spore. in the. magnesium. namely,. acid All. the. and. the. in. contained. sulfulic. in. as. much. mycelium.. mycelium or. natrium, as. were. the. the. mycelium;. temperature,. mycelial. times. potassium,. potassium. inorganic. varied. of the. calcium,. ten. natrium. in. 87. 12%.. the. optimum. in. of. in the. as. the. and. more. than. contents. iron,. silicon,. And. experiment. of. much. variations. increase. much. mushroom･ in the mushroom. The. as. 46%. was. contained. result. oryzae18,19)I. former. 100.. 98. 05. the. found. Aspergillus were. 0.03. 6.18. 95. were. than were. accorded. and. 1.16. ll.9逢. phosphorus, especially, silicon and More phosphorus, calcium and. mycelium. magnesium. 3･. 36. 35. I･`･1. ＋J. the. 0.73. 47. 75. c6. in. 8.73. 1. 49. the was. incubated. the. latter,. in. cool a. the. place. notable. decrease. notable. were. mycelium. a. with a. in. noticed. accompanied. in the. malt-extract. noticed. after. its maximum-growth.. (1). Method. Mycelium. of the. experiment. Collybia. Collybia uelutiPes 67 ∀ar. 女ere used. velutipes 67 and The incubation: were of the i.ncubated on the mycelia malt80ml･ (Ballg･ 9o, PH 5･8, ash 0.138g.) at 24o, in 200ml.-Erlenmeyer :. Method extract. of. jiasks･. They. were. by. determined degree. of Other. a. What. means. pH. methods. periodically.. experimented. pH-determination:. of. of the of. a. test. degree. the. was. paper. it. medium･ the experiment. was. of. on pressed decided were. as. a･. pH. of. the. the mycelium,. and. by?glasselectrode mentioned. above.. was. mycelium as. pH. for. the. meter･.

(15) studies. Crystalline. the. on. of the experiment decrease and periodical. ＼. Mainly. substance. 15. Composed. (2) Result The normal. The. mycelium: was. which. in Table. ･shown. Inorganic. 5.8. 5.8. 5 8. medium. 5.8. 5.8. 5.8. 5.8. 5 8. 1717, Omg.. 1777. 1mg.. 106. 8. 4.1. 3.6. 7.9. 7.7. 6.1. 6.7. 6. 9. 55. 2. 55. 0. medium. mycelium. i. 1.3. 10.1. 10.3. 10.5. 10.2. 10. 4. 14. 2. 14. 4. 13.8. 14, 1. 14. 2. 28. 9. 28. 8. 28. 9. 26. 6. 26. 3. 0.5. 0.5. 0.3. 2,1. 2. 6 8 2. 皿edinm. the. 17o), mushrooms. to. tendencies phorus,. of. the. K20. weight,. potassium. In K20 1ium. was. Na20. 1,1. 1.3. 2. days. were. of. and. and nearly former. the. latter.. It. related. to. was. the. and was. P205, as was. it. when. (3). that,. be. to. the. was. the. quantity as. found. that one. to血d of the. more. period. (15. temperature. to. author. the. of. his. and in. acid. of. third. in. that. and the were. 2･44. to. fungus. percent. the. produced･. myceliu皿･. inorganic. shown. phos-. (1) noticed of Asp･ niger･. O･62. to a. noticed. silicon,. culture. old. amounted. oxalic. has. co-workers22,23). the. little. a. contain. The. and. the. to. come. decrease. in the. mycelium,. cool. younger･. generally. contained. much. has. increased. in fungi. at. mycelial. last. in the. in. pH. develop･. the皿yCelium. magnesium. interesting. growth. to. incubated. the. at. even. natrium. a. at. little decrease. a. and. of magnesium RIPPEL natrium･. content. little且uctuation. noticed. mycelium,. do. 80ml･. (Ballg. 9o) of. extract. found. to. and. content the. their. more. 1.1. cultivation,. increase. the. potassium. (2) that, of. 1.3. soon. it used. than. ash. that,. 4.4. the. were. days. 70. After more. 4.9. potassium ash, silicon, phosphorus, was When the mycelium culture.. weight, ｡f. 4.5. 70. and. medium. 4.6. 3. malt. of. 1 4. 1.2. i. incubation. 55.3. 3. 3 5. 1.3. mycelium. the. 55.. 30.5. 1.2. medium. the. 5. 31. 4. 3.0. mycelium. the. 106. 8. 1.7. medium. on. 105.. 32. 5. 55.0. cnltured. 8. 109.. 2. 109.. 1mg. 1649.. 1708, Omg.. 2mg.. 27. 5. i i. Result. 1762.. 29. 6. medium. both. medium*･. 5.8. i. In. the. and. 5.8. mycelium. ･. mycelium. mycelium. mycelium. Na20. normal. 70. medium. K20. was. result. 60. i. MgO. The. 50. mycelium. P205. medium,. extract. malt. 40. the mycelium. 二SiO2. in the. 30. PI壬. Ash. the. of. components. days. Culture. D.Wt.of. cultured. the. of. components. its maximum-growth･. reaching. on. inorganic. 7.. 7.. Table. was. mycelium. experimented. in the. increase. the. result. inMgO. of. components in. three. contents. similar. AsP1. the. 0ryZae･. as. timesasmuch. of K20 to. each. and. mycebut. P205,. other･. in the. Closely.

(16) 16. s. wakita. The. periodical. varied. and. decrease. of the. Even. in the. normal. mycelium,. mycelium: days,. many. long. of the former･. the. as. little decrease. a. components. the. increase. quantity. When. was. 8･. Table. Change the. of. in. the. varied. days. The. content. of. mycelium. 30. in. for. inorganic as. cultured. to. variety. wide. is shown. result. the. the. a. the. cultured. was. gave. inorganic. and. was. mycelium. the mycelium. components.. it. noticed. varied. in. components. when. were. the. that. noticed. its inorganic. of. Culture. and. mycelium･ it. increase. inorganic. in. Table. 8.. componelltS. medium*.. 40. 戸. 50. 60. 70. __.rーー_､ーーrl_∼. mycelium. 5.8. 5.8. medium. 5.8. 5.8. i. pH D.. Wt.. of比e. 802.. mycelium. 81.1. medium. 73. 1. 69. 3. 57. 3. 1.5. 1.7. medium. 10.1. 8.5. mycelium. 35. 7. 36. 6. i. medium. 20.3. 19.5. 6.1. mycelium. i. MgO. medium mycelium. 12. 9. 13. 1. medium. 15.9. 14. 7. i i. E20. rr]. Na20. ycelium. medium. *. Result. In 40. the. mycelium,. day's. But. the. throughout. days. increasing as. nearly. ammonium tion. as. equal. normal. 12.7. 13. 0. 12.1. 20.4. 20. 9. 16.1. 12. 3 9.3. 4.6. 3. 9. 6.0. 18.5. 4. 9. 21, 8. 22. 4. 3.1. 2.2. 2.1. 3.0. 3.9. malt. (Ballg, 9o). extract. increase. to. lasted. of phosphide its. however,. 80ml.. of. more,. contrary'magnesium. the. end. decrease. gradual. once. to. up. after was. constantly. of its culture-especially. a. noticed.. day,s. 50. its. of. was. culture an. showing increase. rapid. after. of either the. ratio. phosphate･. ammonium. magnesium. or. the of either of In that period,. magnesium. in the. phosphorus two. therefore,. is. phosphate. mycelium in. contained. surmised. the. crystalliza:r. be. to. normal. on. carried. mycelium.. The. percentages. in Figure. shown. After. rus. 26. 0. 1.6. period. ratio. magnesium. of. in the. spite. 白.8. 43. 4. of culture.. The was. [ i 2….･… 43:.･'3S. 1.7. the. the. 1.1. 3.9. which,. On. 58. 7. 4.0. came. mycelium･. increase. 50. after. pbosphide. 1mg. 79. 4. 3,8. increase. an. culture,. this. of. the. on. cultured. 699.. 1. 8.6. 18. 3. 6.5. 2mg.. 68. 0. mycelium. P205. 827.. 65. 1. i. SiO2. 1086. 2mg.. mycelium. i. Ash. Omg.. 7.0. [言:芸 66.'27. of. 40 the. released. of. inorganic. to. components. the. mycelial. weight. are. 7.. days. the. of culturing. decrease into. the. the. of potassium, medium. by. mycelium, natrium. the. ash. and. autolysISOf. was. seen. silicon, the. because. mycelium. to. increase. the was. phosphoaccumu-. in.

(17) Studies. 1ated. in. there. the. coworking ln. this. ledgement. led. that. were. and. decreasing the. of. increase. shown. Figure. 8 shows. inorganic. 1ium. stages. RIPPEL the. as. in. mycelium the. cultured. heated. 4.. on. neutral. medium.. in. /. 1. The. I-. mag-. more. than. unsoluble. component. found. was. and. room,. a. 3o. in. Change. With. neutralization. of. been. relations. When. the. reached. its. noticed. in. with. maximum･. a. days. percent. of. the. of. mycelial. dissolution. Df. the. cell. part. in. into. its culture,. mycelial. increase. of. a. is. no. doubt. that. for. days,. of. varied. rr]ycelium). a. mush-I that. wonder. it. this,. that with. is. con-. natrium. it. controls. its growth23).. of. object these. i亡. aS. mushroom,. potassium,. the. with. creating. materials. will_. growth.. 24o. at. in. On. the. and. decrease. 70. the. it. contrary,. the皿yCeliu皿had. by. autolysIS. after. mycelial of its. its. And, and. a. their. growth. components. was.. that the noticed inorganic components.. weight. in its maximum-growth･･ component. of. No. of. material･. magnesium. incubated. great. content. forth. Besides. transportation. a. it may'it. mycelium･. weight. development. little丑uctuation. a. 70. of. a. were. normal. showed. the. it.. towards. moved. it is discussed potassium, having important relations. as. wt･. it put. when. to. made. that. mycelia. the. regard mycelium,. Be. carboxylases24,25)･. one. a. have. Inquiries. close. takes. phosphorus. D･. (%,. and. for. necessary. 70っ. 如. the. of. abund_. components. component. inthe. components. mycelium. predevelopedrnycelium,. be. SO. Days. phosphorus, were. these. of. magnesium･. After. the. important that. sidered. that. should. an. varied. 伽. there.. potassium. quantity. phosphorus. have. 也. 0. is. Halo. &. substance. Fig.. 7.. contained. Some. :姦. 1. Siqふ_. crystalline. inorganic. antly. 64. ラ. is. niger. that. experimented. the. magnesium. a. much. inorganic. lt. and. ＼.3/. 斗. Consideration. forms. S. ” ￠ Pl. ＼. ever. water. other. /. .. 4) O. as. medium,. in. nesium. 6. contents. acidic a. 召. myce-. to. times. ＼〆. †. this. all. if Asp.. has. author. any. in. According. MgO. three. are. the. of. throughout. growth･. co-workers22),. and. in. its. of. the 9. percentage. increasing. seen. /. IO. on. contained in the contained. MgO. was. the. a. .. percent-. still 7.. Figure. magnesium. substance.. II. components. The. ash･. in. the. and. and. ash. A is. therefore,. ash,. phosphorus. increasing. respectively･. as. of the. 17. acknow-. of its. gradually. Composed. the. after. the. the weight. carbohydrates. age. to. Mainly. crystalline. mycelium,. maximum-growth,. Suもstance. result. a. produce. the. way,. the. as. 皿yCelium, to. Crystalline. the. on. was. lessened in decrease. as. result, of. far. as. both. a. mycelial.

(18) S. 1Vakita. 且8. 6①. ･＼.///. 50. 1. ー一I-I. ヰふ. g. ノーー. ′一一ノーー､. oo. 30. h ￠ EL. ｢､. ---･-/. /′. 3. -.. ,. /. ∼-----㌔. う I-_-/_-r=_-=, <r }0. ノ♪くt}くニー--. ;.,i-::ニンノ Z. ヽ. ＼. ､●. ､●㌔. 5 -e･-_. ーヽ. こ--￣￣--e-. -￣. -t盲<::≡. 10 Ll. _ノ｡. h句己こここ. め.i. ___----_■■_■■■._-. ■■-. ■￣■叫. 5 ___. _. ▲■.,I_. :互:=つ. -_.. .._-4･一-_. 0. 30. 'TO. GO. EO. 年O. Days Fig.. 8･. in. the. in. ash)･. the. Of. components 2, MgO;. 1･ P205;. varied. 4･､Na20;. 3,K20;. ---一皿edium･. of the. must. have. brought. are. gradually-sooner. component. Of. the. out. decrease. above-mentioned. the inorganic speaking, or later-decomposed. Generally. mycelium･. mycelium. a. inorganic. of. content. (.%, in. medium. respiration. weight. ･stored. the. -皿yCelium, by. ℃omponent. the. and. mycelium 5, SiO2･. in. Change. substances. maximum-growth. phosphorus-a. in the. there. magnesium TnyCelium, in words, other ･whicb,. the. when inorganic in. with. found. was. _it :rose,. the. more. Magnesium magnesium. in. the. the. magnesium. of the. course. actively. did not previously. in the. acid. phosphoric. co-operation. being. into. came. ammonia. this. into. acid. phosphoric as. the. and. crystallization. was. carried medium･ a. after. decomposed. were. in. in. the. the. the. medium.. mycelium,. mycelium,. that. the. substance,. crystalline. of ammonia. appearance. the. more･. medium･. in. ammonium. into the abundantly into the medium･ dissolved exude. a. produced. medium. and even. melted later. mentioned. the. in. more. compounds. phosphoric. into. components. a皿yCelium. the. its. substance. crystalline. increased. into. dissolved. organic. inorganic. the. and. enzymes,. gathered. gradually being without the. but. decrease. not. a. inorganic. other. was. its maximum-growth, later-mentioned, As by. did. into. collected. above-mentioned, the from Different. mycelium･. dissolved. mycelium-was. aS. agaln,. and. medium,. the. after. the. more. and. pH. out･. Along. little magnesium. with. much. exuded.

(19) Studies. into. the. have. by. medium. in. insoluble. the. 二1ike other more. more. and. forming. a. 6H20,. ratio. was. which. than. the. in. crystalline A. the. culture the. ′ash whether. the. acidic. LWill be. medium. the. ln. ･especially be accumulated. As ･will. a. the. for. ､sary. the. In the. varied. 了ormerly rus,. ammonium. ･for this. development. would to. vcame. its. after. ･mycelium the. and. to. crystalline. ニSeCretions ･-made. of. chiefly. one),. exude. of. will lie. to. oxalic. acid26).. magnesium on.. potassium little in be. will. an. the. were. the. postneces-. very. mycelia･ of. the鮎1d. of in. release. autolysIS. medium,. and. all. development. mushroom. in the. ･necessary. Phosphides. Mycelium. the. livers27),fishes28(,. the. the. as. phosphoIn the varied. components. into the. then,. and. of magnesium,. abundance. Decrease. and. maximum-growth,. medium,. thus. lncrease. its. after. the. consisting in amatof. produced. maximum-growth,. nucleotides into. oxalate. and. relativelya. mushroom exuded in the fact that resulted di氏culties in its course.. to. acid. happens. goes. components. into. substance. were. others. Periodical. regard. the. of. ｡Ⅹalic. mycelium,. incubation. and these. with. existence. magnesium,. of. weight. decrease will notably Generally, oXalic acid. acid. in the. the. as. the. magnesium. in the. (the sterilized. of. With. the. beginning. phosphoric. have. with. III･. if. especially. that. phos-. when. is in. the. is combined. mycelium. found. of. probably meet. in. And. solution･. more. the. even. of皿uShrooms.. were. the. neutral. phosphorus,. mycelium. related,. medium,. one. accumulating. and. Because. difference. no. magnesium. be suspected. production. ￣these components. the. little less. a. reached. a. mycelium.. and. pre-developed. it may. one,. ･developed. more. the. and. produced,. that. result. in. or. substances. Increase. abundantly. the. remarkably,. Why?. into. on.. the mycelium is MgNH4PO4･. most. experiment. that. mycelium,. be. of such. probably. produced. this. shows. alkaline. the. Will. result. From. niger. ammoniac. MgNH4PO4. was. was. and. acid phosphoric and to 60 day,s culture, of 50. happens, the autolysis ash increase in the one･ neutral. Asp.. into. experiment. am-. magnesium,. went. of which. between. the but. by. accumulated. and. medium. into. component. an. in. is acidic. when. produced. there,. niger22). medium. that,. and. ･culture,. culture. exuded. produced. Asp･. its. Bgure. was. was. of. the. of theory-3･911. mycelium. substance. showed. as. probably. acid. that. into. substance the. phosphoric. exuded. mycelium,. But. above-mentioned丘gure. acid. -phoric. the. would. not. main. 19. membrane,. mycelium,. the. crystalline 3･6･ And. Composed. experiment. magnesium in. cell. are. PO4/Mg-3別･. the. which. the. substance,. be. will. during. and. increasing. ratio,. magnesium. in. acid. crystalline. the. did. into. accumulated. Mainly. combifling. the. so,. components,. If phosphoric. the. of. compounds,. And. mycelium･. inorganic. Substance. dissolution. the. accumulated. monia. Crystalline. the. on. of Of. the. inorganic. organism,. yeasts29･30),. but. some. scarcely. phosphoric inquiries Into. acid have. mushrooms.. or. been.

(20) S. Wakita. 20. previously for. ized. years, then. line. that. they. For. 且uctuated. with. Method. the. of. Mycelium. 6.0). The. :. tween丘1ter in. a. by. colorimeter. Measurement to. the. crystalT-. phosphoric. acids. an. into. it. O･7g･. of the. analysis. The. 24o･. water, of. was. down. crushed. phosphides･ fractionated. was. phosphides,. acid-soluble. be-. nlpped. was. mycelium. crushed. (Ballg･ 7o,. malt-extract. at. with. mycelium. for. used. method inorganic. the. phospho-1ipides,. total. the. and. total. a. of. photocell. was. crushed皿yCelium were. phosphides. was. phosphide. of 660m〃. D･. Q･. The. :. phosphide. =CIO4,. percent. by. measured. Inorganic. :. acid. phospholic. and. method. of the. in 60. ashes. medium,. phospho-proteins.. of ALLEN's32). by. and. 200ml･. of. dehydrated. modified. Measurement treated. forming. organic. the. on. mycelium. the phosphides:. and. acids. incubated. the. The. of. ScHNEIDER,s31). nucleic. the. the. analyzed･. was. washing. (ice-cooled). separation. into. mycelium･. for. extract. malt. compounds. the. Erlenmeyerjiask. papers･. mortor. the. steril-. velutipes 67･. an. After. sampling:. were. mycelium. in. 40ml.. of. in. was. which. experiment. Collybia. :. Incubation pH. of searching days culture. the. culturing. on. of MgNH4PO4･6HoO･ for this fact,. composed. purpose. mycelium. phosphoric. accuiTlulated. mainly. the. mycelial. were. varied. by. development the. the. that. noticed. exuded. substance. 1.. author. the皿uShroom. several and. the. reduced as. analyzed. the. above. Measurement in. lOOo in. the. amount. the. of. total. inorganic 2.. inorganic. the. of. weight. 6n. the it. between. relations. soluble was. as. shown. the. that. and. phosphides. ScHNEIDER's. method:. previously. was. the. Brie且y. concerned.. The. was. at. difference as. shown. the. between. difference of the. organic. phosphoric. As. method. A7-P. and. the. acid･. experiment. tissue,. animal. acid:. and. phosphoric. organic. acid. the. of. after. boiling. NH2SO4. The. tested･. was. acid. before. in. treated. was. sample. phosphoric. acid. acid. of. phosphoric. Result. the. A71P.. (1) Tests an. the. phosphoric. Measurement the. A7-P:. 7minutes, inorganic. When. of the. times. speaking,. to. applied extract. of. and. the the. the. so. mycelium, amount. by. made. the extractions. was. the. of. devised. for. far. the. as. were. extraction. ScENEIDER's. method the. total compared with respectively'when times by the same (a) 93･58% method･ repeated丘ve result of the extraction in nucleic in phospho-lipid-P; in acid soluble-P; (c) 75･92% acid-P; (b) 95.03% last one, was in phospho-protein-P. And the above, very especially (d) 41.05%. showed. following. the. ･1ittle. Then, is, this That 93.58%. of. the. in the extraction. percentages. later. experiment, was. above-mentioned. repeated. 5. the. ScHNEIDER's. 3 times extractions;. in acid. 4. method. soluble-P, times. in. was. modified･. amounting phospho-lipid-P･. to.

(21) studies. 95.03%. to. amounting. Crystalline. the. on. the. of. to. 91.97%. of. the. same. amounting. to. 86.90%. of the. same. increase. (2) Periodical A.. Comparison for. incubated. In the. mycelium･. 9.. Table. in. Days. inorganic-P. Acid. i. Soluble. organic･P total-P. Pbospbide Pbospbo. acid-P. Phospho *. Percentage. to. the. rorganic-P,. days. was. ･of organic. B.. in. was. of. culturing. of. the. 74. at. the were. 95. 67. 56, 95. 57. 09. 8.10. 2.53. 0. 48. 7.12. 23. 74. 14, 79. 2.ll. 24. 10. 2.15. 1.75. 7. 83. 7. 14 23. 28 8.50. phosphide.. the. and. As. nucleic. for the. in the. decreased. acid-P. normal. but. of phosphide. remarkably. the. on. mycelium,. contents. of the in of. contrary,. in the. the. 0.6%. the. increased gradually.. the. afterwards,. mycelium. a. a. any. contents. to. amounted. 24th. And. days. the. the. of. surface. remained. in the. phosphides. medium.. 17th. greatest date. The. medium in. this. the. the. of. same. its life･. On. the. day. mycelium medium･. in. quantlty. culturing-the the. age. the. the. when. of. this. age･. reached. greatest. at. weight of. mycelium. life,. their. mycelium. organic. a. which. whole. throughout. weight. initial) which but. the. on. the. third. other in still remained its. throughout. mat･. one. of. periodically･ in dia8cm･. analyzed. round. The. :. mycelium. in any. mycelium. extended. of. a. about. phosphides in. maximum. its. and. into. grew. of. acid-P. date.. the. extract,. malt. phospho-lipid-P. that of. reached. of. the. of thisagewas. seen. maximum. the varied. phosphides貯in. mycelia. organic-P. that. after. (about. creased. 69. 53. nucleic. the. and. and. sion,. 57. 20. percent. age. having. mycelium. 4如g.. 23. 79. group. showed. same. 』7-P. found. 33. 22. mycelium. of the. amount. before. 0.00. cultured. the. about. roccasion,. The. 15. 80. maximum-growth, in quantity than. greater. The. 90. 30. 28. 60. noticed. Thoughthe one. 闇as. 90. 67. velutipes. phosphides.. varied mycelium In ten days,. the. C.. ∀ar.. 33. 30. Periodicalfluctuation. meter.. the皿yCelia*･. of. L. the. 23. 73. passed･. not. mycelium. 95｡ 67. phosphollipid-P,. of culture. 丑uctuation. 67. 50. l･ 96. total. the. content. noticed that. 53. 73. i. protein-P. varied. normal. 28. 60. lipid-P. Nucleic. as. 30. incubation. of. Little. was. notable且uctuatio･n. celutipes. mycelium. above. in the. phosphorous. mycelium:. The. mentioned･ in the of phosphides as. contents a. the. mycelium:. varied. discerned. Collybia. Mycelia. the. however,. it was. Change. acid-P･. nucleic. phospho-protein-P,. phosphid甲in. analyzed. in. one,. words,. the. were. noticed. varied. In other. 9).. (Table. was. of the. and. days. 90. in. extractions. decrease. normal. 50 and. increase. or. ･decrease. 30,. 21. in. times. 4 times. extractions;. and. the. of. Composed. 3. extractions;. same. amounting. Mainly. Substance. the. quantity was. also. phosphorus on. this. mycelium. of occa-. de-.

(22) 22. S. Wakita. 2.o. J.8 a. 弓l･&. /. ∼ r･1. I.Q. oo. ら a I.1. ∼.0 ●. l.0. J.). a F). 0.8. /. _. 浪. ＼. ＼. ＼.. /. :㌔ ●＼ //:. _■l一---一--一. ＼. ＼. Z'. ＼. こゝ･｢く. /. 0.i. _●. I-＼●. 5. ＼. ミニ≒王. 2. ＼ン二 /. Q.6. Aサ. ○. ./. ー. _■■----■■--■一. ●■. Q＼｡. /. ㌔_申. 7. 8. ○ J O. 3a. 3年. J1. 38. 5え. 4ぎ. 66. Day王∋ 9･. Fig･ 1,. drop. organic-P;. surface. of the. the. ;. of water, The. least. totaトP of. 8,. varied 4, 』 7-P;. a. to. the. mycelium. 5, nucleic. phospho-protein-P.. for 38 days. cultured. its surface. phosphides. the. of. phospho-lipid-P;. mycelium. of various. content. inorganic-P;. 3,. trace. was. crystalline. of. substance. is shown mycelium. of the. weight. little. a. noticed. 9.. Tbroughout the. 7,. underside. and. ratio. in Figure. in pbosphorous soluble-P;. 6,. acid-P. On. Change 2, acid. total-P;. days. 66. of inorganic-P. the. of. the. culturing the. on. little,. was. it. mycelium,. 24th. In. day･. the. found. was. younger. to. contain the. mycelium,. it became. 31st. comparatively the maximum the day and it decreased after culture, which till it reached the minimum gradually the 45th day･ After that, however,showed a increase･ The steady phoson. its. on. pho-lipid-P. and. the. mycelium,. but. after. the. at. the. beginning. 45 days. most was. which. From. the a. showed this. to. the. beginhing. fact. is safely. considered,. this. will. way. the. form. 』7-P of. that. culturing it reached until. the. therefore, a. Period. organic that. during. percent. percent. its. days. which. of the. mycelium the. 』7-P. was. 24. days,. for. total. inorganic-P. minimum. the. on. up. to. composed. culturing organic. about. the. lasting. phosphide 24. started,. the. of. was. shown. mycelium. cultured. mycelium,. two. quantity. the. of. younger. these. of. phosphides,. mycelium. decrease. this. of the of the. all the. 45 the. that. seems. 23. each. fourth. one. the. ln. occupylng. mycelium,. incubation. Of. especially. decrease, it. decrease･. swift. abundantly,. And. to. Since the culture･ been keeplng 22 to a. found. gradu･al. culture･. owing. of. acid-P it showed. when. recognized in. its. in the. much. comparatively this. of culturing to have decreased. had. nucleic. were. 17 days. found. Were. components. phospho-protein-P. phosphides. in. 24th. this. day. day. it of. was. vigorously. the. phosphide. mycelium is enriched. It in.

(23) studies. the. with the a. mycelial. 3.. not. the. varied. the. to. up. were. day. 45th. in. pBosphide there. phosphides. organic. the. inorganic. the. showed the. of. of culturing. myce-. disappeared･. the. the. mycelium. is. to. that. phosphide. will. phenomenon. of. the』7-P, explained. acid. phosphoric. the. reaction. After. that. concluded. paratively. phosphide more. in the. were. found. phosphides.. in the. above, the. and course. nucleic of. its. comparatively. into. were. the. passage. lead. that. and. days. of. in the. The. culture.. after. as. not. were. pbosphide･･〃organic. phosphide-. same. in-. velocity. in the. contents. in. Eorganic. and. nol. phosphide'. of the. way. that. enzyme. its nucleic. Another. acidexperi-･. were. mycelium cultured. com--. days･-. for 70. organic･ of the acid soluble became more and remarkable. the丘rst. the. fact. the. it had. phospho-protein. it grows,. swift. organic. by. mycelium. mycelium That in them･. recognized. of. decrease. of. of its culture･. mycelium. except. stable. days. 31. other. to. the. acid. activities. old mycelium-the fluctuation in the acid. of. phosphides. process. the. with. will. an. mycelium. of. reaction. phosphoric. nucleotidase. especially,. mycelium, and no fluctuations medium. the. remarkable. mentioned. the. with. a. stage･･. comparatively-. a. the. and. in. this. any且uctuations. its maximum-growth,. reached. mycelium. gradually. ･decrease ment. the. the. of'. days･. of A7-P,. isolate. tbis･. in. Of. showed. place. in. its best. 〟. therefore,. contents. to. seems. else. take. days. and. After. through. the. 31. than. result,. mentioned in the is found. nothing. And. of the. early culture decreases. a. phosphide〃･. separated. will. more. above. fluctuation and. for. cultured. mycelium The. acid=. quantlty. yet. phos-. phosphide. also. contained. nucleic acid-P follows :. as. at. maximum. phosphideH. the. acid･=. ▲一一. Lp-r. be. great. and. of. days.. stage a. and. be. will. nhosnhl･de-^7-P-phosphoric qJY ￣￣【ー￣ー￣』7-P, and 』7-P-phosphoric r. reaching. increase,. be. a. younger. ”organic an. 24. should. =organic. the. or. the. contents. its. phosphide. mycelium,. the. That. in. as』7-P. inorganic in. noticed. This. of. the. the. to. the. it gradually. for. analyzed. contents. number. in. 10 days,. were. inorganic. of. remarkable. mycelium. its weight. inorganic. such. culturing. of the. growth. the mycelium. other words, little quantity. but. the. the. the. content. for. ascribed. mainly. energy. the. cultured the incubation after. minimum. In. high. Though. mycelium. the. fact 1S. stage,. according. to. different. was. phosphides. they. in. notable且uctuations. comparatively. recognized. When. in totality･. analyzed. mycelium･. mycelium-the. reaches The. were. their. of. notablefluctuation. however,. recognized-each. culturing. the. any. they. phosphides,. various. of. the. while. phosphid白”. mycelium,. when. recognized. phides. As. this period,. 23. Composed. Consideration ln. in. "organic. Mainly. After. lasted. which. the. when. increase,. to. decrease,. steady. lium,. components･. came. mycelium. Substance. Crystalline. the. on. the. and stage. of. phospho-lipid culture. its maximum-growth,A reached is, the phosphides moved. of. turned. mainly. into. the. the. from organic･.

(24) 24. S.. After. its maximum-growth,. and phosphide once, began to. above, but. time. out. the. of. some. this. ooze. isolate the. of. had. mycelium the. phosphoric. rnycelium. phosphoric. the. as. the. acid. nucleic. Wakita. acid. crystalline. soluble them,. into the. gathered. compounds. acid from. acid but steadily.. slowly. there. its. at. which,. As. mentioned. mycelium. composed. mainly. organic. again-. MgNH4PO4･. of. 6H20. Accordingly, tured rus. 70. about but. between days-is. hardly. any. the. younger. noticed. a. its. ln. large. the. and. mycelia. in. difference. older. the. ones-ones. cul-. of. quality. phospho-. quantlty.. be. (To. continued). Referen(:es (1). C.B.. (2) (3) (4) (5) (6). A.. BuELL,. W･H･. DEBARY:. WESTON:. Botan･. M.SIMO:. ∫ Agr･. 44,.!393. Chem･-. Cbemie. M.. Jab. four. Bot., 10,. HAMADA: MELLOR,. J.W. H･. Der. HIRATA:. 387. the. of. (1939). (1907).. ∫(1940). H.V.. Analysis. Inspection. (1947).. 15, 751 Pilze. Sc., F.R.S.,. lnorganic. 34, 555. (1886).. H6heren. D.. C,B.E.,. Bot.,. I.. Soc･ Japan,. ∫.ZELLNER:. Quantitative. (7). Amer･. Ztg･,. THOMPSON,. M.A.:. A. Treatise. on. (1938). internal. material. by. construction. X-Ray.. p.. 32. (1934). 3) (8) (9) )). H･. (10) 0) (ll) 1). W･T･. HAI-L:. V･T･. CHUIKO:. (12) 2). A･W･. EppERSON:. (13) 3) (14) 4). L･W･. (15) 5) 6) (16). E･. RENNERFELT:. AI. DAMBOVICEANU,. (17) 7) 8) (18). R･ ScIINUcHE: K･. As°:. (19) 9). R･. TAKATA. (20) 0). S･ WAKITA. (21) 1). Ⅰ･ MIURA,. (22) 2). K･. (23) 3) (24) 4) 5) (25). S･ OcHOA:. (26) 6) (27) 7) (28) 8). NEUBAUR:. J･ Soc･. J･. :. Agr･. G･. H･C･. BRADLEY:. L･. SASAKI,. T･. Univ･,. 4, 81. Soc･. Japan,. 28, 707. Ml'krobiol.,. Arch･. HERBERT,. 1, 271. Mikrobiol.,. Revs., K.. (1900).. Soc,,. 19, 421. (1937).. 5, 561. I. Biol. Chem.,. Soc., 50, 2630 18, 173. 138, 327. M･. ScHNEIDER: ALLEN:. HIGUCHI. (1938).. FuLUSHIRO,. J･ Agr･. :. T.. Biochem･. Chem･. Soc･. 4抄Ii. Microbiol,,. JosLYN J･ Biol. Chem･, :. J” 34, 858. (1941).. (1928).. TANAKA:. I. Agr.. Chem.. (1953). N･A･. (1931).. (1939).. Chem,. FuJIMAIくⅠ,. 4, 5. (1930). (1934).. Ⅴ･ SuBRA=MANYAN:. Amer.. microbiol.,. (1929). (1954).. Jap･ For.. four.. I., 33, 1262. Physiol･ M･. ZmpI. SAWADA:. et. exp･. (1924).. Chem･. Arch･. Journ･. UEMURA,. R･J･L･. Tokyo. path.. roum･. Japan,. BEHR:. D･. 153, 372. Chem･. :. D･ C･ VosTI, W･C･. (1934).. 32, 497. and. HALL:. (1918).. Znd･. M･. Biochem.. 31, 211. Arch･. Z･,. 343. (1919).. 22, 221. Agr･,. Fac･. I, IwADE,. GREEN,. Chem”. C･ BARBER:. :. W･T･. Chem･. 4, 133. Biochem･. DIE･. Chem.. Angew･. 1%nta,. Jour･. 27, 725. (29) (30) (3 1) (32). Zeit･. I. Bact.,. 16, 289. Chem･,了,. Amer･. Journ･. Soc.,. (1894). (1894). Soc･, 50, 2630 (1928). U･SIS･R., 10, 364 (1937). Chem･ Soc･. 50, 321 (1928).. Anorg.. Journ･ Amer･ Jonrn･ 4抄･. DAWSON:. RIPPEL,. Chem･. Zeit･. :. WINKLER:. A･I･. Amer･. Journ･. P･ SctIOTTLÅNDER. 161, 293. JaI,an, 33, 304 2, 70. (1945). (1940).. (1954).. (1959).. Soc.. Jal'an,.

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