A note on the simultaneous colorimetric determination of pentose and hexose-香川大学学術情報リポジトリ

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Vd.6,No.3一(1955) 241

ANO甘E ON TⅢ毘S‡MULTANEOUS COLOR互M若汀R温C

むETE罠.M甘NÅ椚のNOF,PENTOSE ANわ宜iElX¢S置 BySin,itir6KAwAMURA,TsuneoKOBAYASH工*,andMitio OSIMA*.

(Ⅰ慮0やrybfBioloaical’che払istry)

(Receiもed

In血ecou柑eOfstudiesonsoybean hemicellulosesit was necessa‡y tO determin?Sugar COmpOnentSOnaSmallamountofthepr・eparationobtainedbyhotwaterext貰aCtion(1)・Qlユalitative testsshowedthepresenceofhexoseandpentoset喝etherwithllrOnic.acid・A surveyofli七erature revealedamethodof8imultanqouscolorimetryofp叫08eandhexose一ALBAUMandUMBREtT(9) rq)Qr・teda method to determine$eparately various pentose8and pentose Pho畠p旭es′

甲Onthedifferentdegreeofcolorationinthepentose・・OrCinolreaction・DRURY(3)extendedthis pethodtode七erminecolorimeもrical1ypentosesandglucose・ThismethodofDRURYwasaPplied to the hydrolyzate of hot・Water−SOldble hemicellulose(l)・Though the re9ult obtained was

concludeda$nOtreliable,thi8methodi畠bfinteresto坤g・tOthe畠inal1amountof払eもample neededandto血e‡aPidity.When appliedto suitable samples containing only two monosac− c?ar享de甲(p甲tOSea甲dhexose),theDRU甲m9thムdwi11beofgI.ea!、y′alもゃ・∵掛き寧1Cent reView

ふas

AccordingtoDRURY,glucoseandpentosesbehavedifferently.in払e‥OrCinolreactionunder

頑・tain condit呈on$,andthecolorimetricIeading8atintervals woulqgive払e,PentOSe COntent withina2%・error afterBuitable mathematicaltreatment.Nowweassume that galact6seand

pentosewouldbehaveindependentlywithorcinolunder、theconditions of血edeter血irlation by theDRURYplethodandthat血ecolorimetric亨eading ofthemiⅩtureis9qualto the sumof

the colorimetric reading$Of the components.

Thus 虎=γ・g慮+グ・pen (1) and G=塾頭十鞄en, (2) where RandGarethereadingsofthemixturewithIedandgreenfikers,re8PeCtively,and randgarethereadingsofthecomponents(gal=galactose,andpen=pentOSe)一From(1)and (2)weget 鞄al=

〔.(g/r・)pen・R−G〕/[(9/r)pen・(γ/やgalニl〕

¢) ThereagentS(0。3ml.oflO%orcinolin95.%ethanoland3・Omll10f sulfate,Fe(》鱒4)(SO4)2。12H20,inconcentratedhydrochloricacid)weretakeninthe standard colorimetertube,tOWhichwasadded3.Oml・$amplesolution・Eachtubewas heated atlOOO for45minハandthecolor8Wer・ereadwiththefiltersofred(660mp)andgr貿en(54:Omp)by

thePULFRICHcolorimeter.Standardsl王garS Were di$SOIvedin distilled\Water$aturatedwith ben之oicacidatthe’concentrationoflOOr/ml“Benz6icacid gave no reading、incolorimetry・

(a)Calibrationofardbinoseandgalactose.Two orcin61reagents(Aand 圏FacultyofAgriculture,Univer8ityofTokyo・

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242 Tech小 Bull.華ag・aWaAgI∴Coll. Thedataare summarizedinTablesland2…Forthe orcinolreagent BⅥ屯ich was usedin

(b),ityasdecidedthat(g/r)pen=0・193and(r/g)g可′=11394いForやef甲野nt阜theaverage Of′(&榛)pen±=bl192・

Tablel. Calib王ation of arabinose.

10 20 30 40 50 60 80 100γ

0.2iO O.29i O.385 0.532 0.659 0.040 0.059 0.075 0.098 0∴ほ3 0.柑0 0、202 0.柑5 0ご184 0.王90 0.092 0.222 0.420 0.042 0.080 0.189 0.192 0.062 0.120 0.181 0004 0.012 0..035 (0..065)(0.100) 0.193 ㌢ γ γ γg釘γg釘〆g酎 A B 0.871 0.i68 0.192 0.柑3 こ 3 Table2.C∂‡抽Ⅰ癒ion of gdac乞OSe 川 20 30 40

60 100 150 200γ

No.Q‡Cまnol ‡eagenも 1 A グ 0.067’ 0巾王05 0.169 0238 01rO48′ 0.、074 0.′123 0/1ち1 1.′395、′1.418・1J374′、1.576 g プ/g B プ 0.013 0.030 0.055 0.071 g OuO15 0・022 0・036 0・055 グ/g (0.84)i.363 i.527 1.29王 2 (b)Colorimetryof血ehydrolyzedsample.Thehydrolyzate wasobtainedbyhe?tinghot・ wateI−SOlublehemicelluo19ewith NhydIOChloric acidat1000 foI4 houi$and was analyzed 去fteIneutral主z慮on.Thjs sample′solution containedO.41i3mg.peIml.Thecolorimetricreading$

jn Table3,WheIethe percentages o (asarabinose)and

Table8.ColorimetI 4i.Ⅰ 51」4 白乞3 ほ3.4 O..069 0uO98 √0.173、 0.\274 0.039 、P.052、 01.088 0.148 7. 88 11 I 14・2 王21・5 11・4 170.2 7「2∴5 74.5 86.6 75.9 Sadpユe,γ Reading虎 Reading G AIaもinose,% GalacとOSe,.% Refere王IeeS (l)SKAWJゝMU王も左,TりKoBAYASHl,andMl・OsIMA,おJ4㌢・Cカ♂研い5βC.拗α花,19,69(1955) (2」HG一AIBAUM and.WWU入相REI王,./βよoJ・・C毎朝,167,369(1947−) (3)HF」DRt7RY,A㌢・祓∴ 風ocカ♂雛・,、、19,455(′1949,) (4)SKAWAMUR.よandTKqBAYASH7,.極anAnalysl,3,436(1954) ぺソナーズとへキソーズとを同時に比色定量する方法について 川村億一郎,小林恒夫ヂ大島道雄朋 デイズの熱水可溶性へ・ミセノレロ・・−・女の研究(1)に一おいて少最の試料につきぺ’ソトーズと、ぺキソ†:ニズ せ向時に雇盈するDRU痩せ(5)の方法せ試みたこ グロ・ン艶も共存するので思おしい結果ほ得ら、れなか ったが,試料がペン。トー、ズとへキソ「ズのみを含む場合紅はオルリノ‥−ル反応の墨色を赤と濠粧つ き測竃して計算するこ.の方法は便利である・ 詣 果嵩∴学際学部貌芸化学科

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