C D
0 20 40 60 80 100 120
Cont 50 100 200 500 1000 Ropinirole (M)
Number of Positive Cells (% of Control)
** **
*** ***
B
0 20 40 60 80 100 120
Cont
Talipexole Pramipexole
24 72 72 24 (h)
*** ***
***
**
Orexin MCH
0 20 40 60 80 100 120
Cont 6 12 24 48 72 Ropinirole (1 mM)
% of Control
(h)
**
***
*** *** ***
% of Control
Orexin MCH
Control
Control
Rop (100 M)
Rop (100 M)
Rop (1 mM)
A
Rop (1 mM)
Fig. 1
A
0 20 40 60 80 100 120
Control
Ropinirole (1 mM) 72 h Post Number of Positive Cells (% of Control)
***
B
###Ropinirole 72 h Post Control
24 h 72 h
Control
Control
Ropinirole
Ropinirole
NMDA (60 M)
NMDA (60 M)
C Orexin Orexin/PI Orexin/PI
Orexin
Fig. 2
0 20 40 60 80 100 120
Sulpiride
(M) 50 100 200
Ropinirole (1 mM) Number of Positive Cells (% of Control)
***
## ## #
0 200 0
Number of Positive Cells (% of Control)
Ropinirole Cont PTX Yoh Pind PTX Yoh Pind
*** *** *** ***
#
A
B
0 20 40 60 80 100 120
Number of Positive Cells (% of Control)
Cont WAY
Ropinirole WAY
C
***
***
###
0 20 40 60 80 100 120
Fig. 3
0 20 40 60 80 100
Cont NMDA
Ropinirole NMDA
Double-positive cells (%)
***
***
B C
###0 20 40 60 80 100 120
Number of Positive Cells (% of Control)
Cont MK801
Ropinirole MK801
***
***
***
n.s.
D
0 20 40 60 80 100 120
Cont NMDA
Ropinirole NMDA
***
*
###
Number of Positive Cells (% of Control)
Orexin/c-Fos
Orexin Orexin/c-Fos
A Cont Rop
Cont Rop Rop+NMDA
Rop+NMDA
Fig. 4
