201314年カタログ New England Biolabs Japan

(1)

ດ੍౺ဘߔᅙ૚ᄞȤɢɍʯ˅ʞɼˋʜĜ˃౿ ࿚Ɉ௢੧ɉĪwww.nebj.jpīɬȮઞ௎ȩȺ ȯȞă

■_ޱᅟ

■_{ʈ˂ʋʏʘĜʔɈʾʣʛʠࠓધʙʻĜʠ}

■_{ʈ ˂ ʋ ʏ ʘ Ĝ ʔ Ɉ ʘ ʮ ˃ ʘ ɼ ʐ ɿ ʑ ʠ} ʙʻĜʠ

■_੕ఛ௶ၡ

N /Oय़ਗळດ੍ɈಜඣɈ

ʙʽĜʠ˂ɺ˃຦ކĪܑাīɬ༯ఒෂ Īwww.nebglycosidase.comī

ດ੍౺ဘߔᅙ૚ᄞȤɢɍʯ˅ʞɼˋʜĜ˃ć

ᄝ

₄₀

༃Ɉଌಕɬপɥ౿࿚Ɂȳă

ດ੍౺ဘߔ

౺ဘߔʏʑʞʸɈෂɁɜʗˋʩʇଋɈृຕโĆၟߣโॸ࢞ယᄘɁȜɥʯ˅ʞʂʷʇʑɉʗˋʩʇଋ ཡএĂୌఄĂഊ঱੼ᅙɞ࡙༑ɒɈᆅސɬ߄൥ȱɀȧȹăȱȥȱɄȦɣ፪໳੧ၲɈഽȩɈʗˋʩʇଋ

ɉႩᄟষୌఄȦȯɦɀȞɥĪ1īă੧ၲɈ౺ശɅྼᅟɄێཾโɄႩᄟষୌఄɉດ੍࿥ݡɁȜɥăດ੍

࿥ݡĪʈ˂ʋʏ˃ݛīɂɉ଱Ʌထਗດ੍ȦʗˋʩʇଋɅय़ਗȳɥȭɂɁȜɤĂ੧ၲ࠰ɜȱȩɉ੧ၲ ɂ࿒ঈൌ࠰ɈಞශɈᅟۚɂɄɥɄɃĂດ੍౺ဘߔɅȤȫɥ੕ɜ୭ᅟɄএ௣ɁȜɥă

ດ੍ཡএɬၟߣโɅॸ࢞ȳɥʈ˂ʋʷʇʑɁɉĂດ੍৭ഢɂȷɈ࡙༑Ɉ࠲बɬცɣȥɅȳɥȹɛɅ ହęɈਊ೧ɂယ಑ࡳ୹ȦᅙȞɣɦɥăດ੍౺ဘߔɉྼȴȱɜ൥ȧɄယᄘɁɉɄȞȦ࢖ബɅ౰ෳȱɀ Ȟɥ౺ဘߔယᄘɁȜɤĂ଱Ʌ౺ൌᆽஸۇߔĂ౺ဘ৙ߔĂʨɼʂ༇ᆧɂࡊೣ౺ဘߔɅ࠲ᅇȱɀȞɥă ດ੍ယાɉ੧ၲɞ೨ఉɈယݛɞൣଟĂۆ๙ાཡএ౫সĂʗˋʩʇଋߤ౯ĂʗˋʩʇଋதࢦɞᄪഗɄ

ɃɈഽȩɈ౺ൌ໘ʯ˅ʓʑɬ෮౲ȱɀȞɥĪ2 - 9īă

NEBɉດ੍౺ဘߔॸ࢞ɈȹɛɈێᇦɈʀˋʡʈ˂ʋʏʘĜʔɂʀʅʕʈ˂ʋʏʘĜʔɬอࢷȱɀ

ȞɥăഽȩɈ૚ᄞɉ˂ʋˋʫʢˋʠɂȱɀ౿ഢȯɦɀȤɤĂထౘɈ࿚ଋࠣᆅ૚অȦଌૈȯɦɀȞɥ ȹɛĂ੕਒࿚ଋɂ஋๱Ɉ౿࿚ɬฝȞʋʑʠɁĂȥȾ˅ʛʠ࠰ɈੇڽȦ੕ழঔɅɄɥɢȠɅอࢷȳɥ ȭɂȦݢ༑ɁȜɥăɘȹȳɓɀɈʈ˂ʋʏʘĜʔ౿࿚ɉ࿤஋ဘɅȾȞɀɜউდɅॳੈȯɦɀȞɥă

࿤஋ဘɈ਼໴ॳੈɅɉฆ௵Ăp-nitrophenyl-glycosideȦ઴ᅙȯɦɥȦĂȭɦɉࡐȾȥɈʀʅʕʈ˂

ʋʏʘĜʔɁݡ౉ယސȯɦɄȞȹɛĂNEBɁɉॊ৉ˁʱ˃ȱȹʂ˂ʌʍʛʃˁɼʡȺȫɬᅙȞɀ࿤

஋ဘॳੈɬଌૈȱɀȞɥă

ʯ˅ʞɼˋʜĜ˃

ʗˋʩʇଋɉ౺ൌ໘Ɉ଱Ʉ৭ഢۚાɁȜɥȺȫɁɄȩĂ࡙༑౯ယાɁɜȜɤĂȷɈ௻ൔɅɢȽɀൣ ଟݚᆔɈߤ౯Ȧड़ฤȯɦɥăȷɈȹɛĂ੧ၲ໘Ɉʗˋʩʇଋɬސ಑ȳɥȭɂɉࠞࣃɅൎȳɥ੧ၲܾ ຒ࡙৭ɬცɣȥɅȳɥȭɂɅॆȦȽɀȞɥăᅕęɄʗˋʩʇଋސ಑ɬ਄ȠȹɛɅɉȷɈʜĜ˃ɂၫ ၭȦྼᅟɁȜɤĂ౰োɈȹɛɅɉ౷ȱȞၫၭɬೊ൰ȳɥྼᅟȦȜɥă

NEBɉʗˋʩʇଋސ಑ɈȹɛɈဍঢ়ȞʜĜ˃ɬอࢷȱɀȤɤĂࡊႨโɄʯ˅ʞɼˋˁʘĜĆʶĜ

ʃĜȥɣܾᅙโɄSNAP-tag®_{੧ၲˁʱ˂ˋʈʏʑʞʸɞ}_Ph.D.™_{ʭɹĜʐʟɻʑʯ˄ɼʏʑʞʸ}

ɘɁവȢɀȞɥă

SNAP/CLIP-tagɉᄆโɈʗˋʩʇଋɅĂȜɣɠɥယાɬູڽโɅࢼᄵय़ਗȯȵɥˁʱ˂ˋʈʏʑ

ʞʸɁȜɥăႨʏʑʞʸɉ2ȾɈʑʞʛʯȥɣ৭౰ȯɦɥăɘȴĂSNAP-tagࡊଋɬᄆโɈʗˋʩ

ʇଋɂɈᅃਗʗˋʩʇଋɂȱɀཡএȯȵɥăȷɈষĂᅙ๫Ʌ੕ไɄSNAP-tagɬˁʱ˃ȳɥă

SNAP-tagɉDNAୌဌਊ೧ɁȜɥO6-alkylguanine-DNA-alkyltransferaseĪhAGTīɅࡊȿȞȹழ

ȯȞယાᆲɈʗˋʩʇଋɁȜɥăSNAP-tagɈࡊଋɉʈɺʣˋɜȱȩɉʇ˅˅ʬ˂ʷʐˋɅʱˋʐ

˃˂ˋʃĜɬގȱɀఋ೧ɞॊ৉ĂʫʂʙˋĂʫĜʒȦय़ਗȱȹɜɈɁȜɤĂSNAP-tagɂय़ਗȱȹ

ষɅɉʈɺʣˋɜȱȩɉʇ˅˅ʬ˂ʷʐˋȦၪ୸ȯɦɥăSNAP-tagɂࡊଋɈय़ਗɉĂࡊଋɈʱˋ

ʐ˃ࡊɬގȱȹࢼᄵय़ਗɅɢȽɀ਄ɩɦɥăCLIP-tag™_ɉ_SNAP-tag_{ɬޜᆯȱȹɜɈɁȜɤĂ}

benzylcytosineĪBCīᄾຩൌɂູڽโɅཱܾȳɥăSNAP-tagɂCLIP-tagɉڽɄɥࡊଋູڽ౯ɬ

૲ȳȹɛĂSNAP-tagɂCLIP-tagᅃਗʗˋʩʇଋɬࢼཡএȯȵڽɄɥॊ৉ࡊଋɁˁʱ˃Ī2ఋɁˁ

ʱ˃īȳɦɊĂວێ੧ၲ໘Ɂ2ହᇆɈᄆโʗˋʩʇଋɬວ૪Ʌސ಑ȳɥȭɂȦݢ༑ɂɄɥă௢੧ɉ

10௕ɬઞ௎ȯɦȹȞă

ʭɹĜʐʟɻʑʯ˄ɼɉʗˋʩʇଋɞழယાݛਗဘɄɃɈʗĜʊʛʠယાɅൎȳɥ˂ʄˋʡɬin

vitroɁʑʇ˂ĜʣˋʈȳɥȹɛɈࡳ୹ɁȜɥăʭɹĜʐʟɻʑʯ˄ɼɉʨʇʞ˂ʂʭɹĜʐȦʋĜ

ʠʗˋʩʇଋɅʲʯʙʡɞʗˋʩʇଋɬᅃਗȱɀཡএɁȧɥ༑ᆵɅࡊȿȞɀȞɥăʭɹĜʐ࿍ჶɅ

อ૲ȯɦȹʲʯʙʡɞʗˋʩʇଋɉȷɦɬʋĜʡȳɥDNAɂဘᆅโɅय़ɍ࿥ȫɥȭɂȦݢ༑ɁȜ

ɥȹɛĂin vitroʩʣˋʈɁʗĜʊʛʠယાɂय़ਗȳɥʭɹĜʐɬೊ൰ȱȹষĂʏĜʉˋʑɁູڽโ

༯ᇙɬຶɥȭɂȦɁȧɥă10ݍɬ෰Ȣɥˁɼʮˁ˂Ĝȥɣɩȴȥౘ໲࠰Ɂʑʇ˂Ĝʣˋʈȳɥȭ

ɂȦɁȧɥȹɛĂ഼Ɉʑʇ˂Ĝʣˋʈၭڬ௫ɅʀʬʠĜʯʶʛʬˋʈɞ˄ʓʯʗĜ˂ʄˋʡວฤĂ

ȷɦɅʗˋʩʇଋమݛɈȹɛɈᄵᆵɄʑʇ˂ĜʣˋʈၭɁȜɥăNEBɈPh.D. phage display

library kitɉʲʯʙʡɈʨɼʂʩʣˋʈȦݢ༑ɁȜɥă

(1) Khoury G.A. et al. (2011)

Scientiﬁc Reports

1: 90. PMID: 22034591.

(2) Varki A. (1993)

Glycobiology

. 3(2):97-130. PMID: 8490246.

(3) Zhao Y.Y. et al. (2008)

Cancer Sci.

99(7):1304-10. PMID: 18492092.

(4) Zhao Y. et al. (2008)

FEBS J.

275(9):1939-48. PMID: 18384383.

(5) Skropeta D. (2009)

Bioorg. Med. Chem.

17(7):2645-53. PMID: 19285412.

(6) Neu U. et al. (2011)

Curr. Opin. Struct. Biol.

21(5):610-8. PMID: 21917445.

(2)

9

ດ੍౺ဘߔ

_&

ʯ˅ʞɼˋʜĜ˃

Endoglycosidases

Glycosidase Double Digest Chart 218

Endo H, Endo H_f 219

PNGase F 219

RNase B 219

O-Glycosidase 220

Remove-iT PNGase F 220

Remove-iT Endo D 221

Remove-iT Endo S 221

Protein Deglycosylation Mix 222

Fetuin 222

Exoglycosidases

α-N-Acetylgalactosaminidase 223

β-N-Acetylglucosaminidase 223

β-N-Acetylhexosaminidase_f 223

α1-2 Fucosidase 223

α1-3,6 Galactosidase 224

β1-3 Galactosidase 224

β1-4 Galactosidase 224

α1-2,3 Mannosidase 224

α1-6 Mannosidase 225

Neuraminidase 225

α2-3 Neuraminidase 225

Detailed Characterization of Several Glycosidases 226 - 228

Heparin

Lyases

Bacteroides Heparinase I, II, III 228 - 229 Heparin Hexasaccharides MS Standard 6, 7 229

Phage

Display

Ph.D.-7 Phage Display Peptide Library Kit 230 Ph.D.-12 Phage Display Peptide Library Kit 230 Ph.D.-C7C Phage Display Peptide Library Kit 230 Anti-M13 pIII Monoclonal Antibody 230

pMAL pIII Vector 230

M13KE Phage 231

Ph.D. Peptide Display Cloning System 231

Proteome

Analysis

Trypsin-digested BSA MS Standards 232

Proteases

Trypsin-ultra, Mass Spectrometry Grade 232

Endoproteinase GluC 233

Endoproteinase AspN 233

Proteinase K 233

Factor Xa 234

Enterokinase, light chain 234

Furin 234

Protein Phosphatases & Inhibitors

Properties of Phosphatases from NEB 235

Lambda Protein Phosphatase (Lambda PP) 236

Protein Phosphatase 1 (PP1) 236

Protein Phosphatase Inhibitor 2 (I-2) 236

Protein

Kinases

cAMP-dependent Protein Kinase (PKA) 237

Casein Kinase I (CK1) 237

Casein Kinase II (CK2) 237

Glycogen Synthase Kinase 3 (GSK-3) 237

CDK1-cyclin B 237

Calmodulin-dependent Protein Kinase II (CaMK II) 237

p42 MAP Kinase (MAPK) 237

(3)

Glycosidases

■_{ᅕęɄଌঅɅ઴ᅙݢ༑}

■_{ʾʣĜʇɄູڽ౯}

■_਒࿚ଋ

■ _਒஋๱

NEBɉດ੍౺ဘߔɈȹɛɈᅕęɄʀˋʡ / ʀʅ

ʕʈ˂ʋʏʘĜʔɬอࢷȱɀȞɥăȭɦɣɈ ഽȩɉ˂ʋˋʫʢˋʠ౿࿚ɁȜɥȹɛĂʾ ʣʛʠඍݟȦྥ௵ɅฝȩĂ਒஋๱ȥȾ˅ʛʠ ࠰ɈɊɣȾȧȦவɄȞȭɂȦູෳɁȜɥă

೔ɀɈʈ˂ʋʏʘĜʔɉʋˋʗʷʥĜʏʿˋ ȦɄȞȭɂɬߊ໻ȯɦɀȞɥă୦ᅰ઴ᅙȯɦ ɀ Ȟ ȹp-nitrophenyl-glycosidesĪPNPī Ȧ ȞȩȾȥɈʀʅʕʈ˂ʋʏʘĜʔɁɉݡ౉ယ ސȯɦɄȞȹɛĂॊ৉࿈ૻȱȹʂ˂ʌດȺȫ ɬࡊଋɂȱɀʈ˂ʋʏʘĜʔɈʋˋʗʷʥĜ ʏʿˋॳੈɬȤȭɄȽɀȞɥă

NEBɈʈ˂ʋʏʘĜʔɅɉȳɓɀ੕ไʨʛ

ʭɹĜĪX10īȦ࿥മȱɀȞɥȹɛĂ૖ไߤ

౯Ȧຶɣɦɥăଌঅ૪࠰ɈಣᄝɈȹɛɅ2ହᇆ

ڬ௫Ɉʈ˂ʋʏʘĜʔɬວ૪ɅཱܾȯȵɥĪʘ ʮ˃ʘɼʐɿʑʠī௲ਗĂਊ೧ߤ౯ɅɂȽɀ ૖ไɄཱܾ௸ॣȦຶɣɦɥʨʛʭɹĜɬೊ൰ ȳɥȭɂȦ୭ᅟɁȜɥăʘʮ˃ʘɼʐɿʑʠ Ɉ૖ไ௸ॣɅȾȞɀɉĂݚࡥʙʻĜʠɬઞ௎ ȳɥă

ʈ˂ʋʏʘĜʔɈʘʮ˃ʘɼʐɿʑʠʙʻĜʠ

ʹʺćS = ဿ౯௸ॣɁஏྈɅཱܾɬ਄ȠĪ1ȾᄆɈਊ೧ɁஐᆅɬȱȹষĂ༂଄ߤȤɢɍʨʛʭɹĜɈဿ৩ɬ਄Ƚɀȥɣ2ȾᄆɈਊ೧Ɂཱܾɬ਄Ƞīă

ȞȴɦȥɈਊ೧ȦBSAɬྼᅟɂȳɥ௲ਗɉʘʮ˃ʘɼʐɿʑʠཱܾʨʛʭɹĜɅ๒ݡȳɥă

NEB #

P0704

P0705

P0706

P0702

P0703

P0741

P0742

P0733

P0726

P0730

P0731

P0732

P0734

P0721

P0729

P0727

P0720

P0728

P0724

P0704

P0705

P0706

G7

S

G7

P0702

P0703

S

G5

S

G5

S

P0741

G7

G5

G6

G7

G6

P0742

G7

G5

G7

P0733

G7

G5

G7

P0726

G7

S

G6

G7

G2

G6

P0730

G7

S

G6

G7

G6

G4

G6

P0731

G7

S

G6 G7

G7

G6

P0732

G7

S

G6

G7

G6

G1

G6

P0734

G7

S

G6

G7

G6

G7

G6

P0721

G7

S

G6

G7

G6

G2

G6

P0729

G7

S

G6

G7

G6

P0727

G7

S

G6

G7

G6

G2

G6

P0720

G7

G5

G6

G7

G6

G1

G6

P0728

G7

S

G6

G7

G6

G4

G6

P0724

G7

S

G6

G7

G6

G4

PNGaseFPNGaseF (Gl ycer

ol Fr ee)

Remo ve-iT

™ PNGase F

Endo H, Endo H

f

Remo ve-iT Endo S

O-Gl

ycosidase β1-3 Ga

lactosidase

Remo ve-iT Endo D

β1-4 Ga lactosidase

α1-3,6 Ga lactosidase

β-N-Acetylg lucosaminidase

α-N-Acetylg alactosaminidase

β-N-Acetylhe xosaminidase

f

α1-2,3 Mannosidase Neur aminidase

α2-3 Neur aminidase

α1-2 Fucosidase α1-6 Mannosidase

ཱܾʨʛʭɹĜ೨౰ć

1X G1 Reaction Buffer:

50 mM Sodium Citrate (pH 6.0 @ 25°C).

1X G2 Reaction Buffer:

50 mM Sodium Citrate (pH 4.5 @ 25°C).

1X G4 Reaction Buffer:

50 mM Sodium Citrate (pH 6.0 @ 25°C)

and 100 mM NaCl.

1X G5 Reaction Buffer:

50 mM Sodium Citrate (pH 5.5 @ 25°C).

1X G6 Reaction Buffer:

50 mM Sodium Acetate (pH 5.5 @ 25°C)

and 5 mM CaCl

₂

.

1X G7 Reaction Buffer:

50 mM Sodium Phosphate (pH 7.5 @ 25°C).

糖鎖生物学＆

プ

ロ

テ

イ

ン

ツ

ー

ル

(4)

Endo H

f

Endo H

#P0702S 10,000 units . . . ._{¥ 12,000} #P0702L 50,000 units . . . ._{¥ 48,000} Endo H_f

#P0703S 100,000 units . . . .¥ 12,000 #P0703L 500,000 units . . . .¥ 48,000

ತცćEndoglycosidase HĪEndoHīɉĂ਒ʶˋ

ʦĜʑɈʅʠʫʂĜʑʋɺ໘ငɞNय़ਗळດʗˋ

ʩʇଋɈʧɼʮ˂ʛʡʂ˂ʌດ໘ငɁດ੍ɬಜ ඣȳɥ˂ʋˋʫʢˋʠʈ˂ʋʏʘĜʔɁȜɥă

Endo Hf ɉĂEndoHɂʶ˃ʠĜʑय़ਗʗˋʩʇ

ଋɂɈᅃਗൌɁȜɤĂEndo HɂວێɈߤ౯ɬᄵ

ȳɥă

ᄻᅰćEndo HȤɢɍEndo Hf ɉĂStreptomyces

plicatusȥɣʇ˅ĜʣˋʈȱɀĂ൥෬ࣳ໘Ɂݿ௰

ཡএă

ཱܾ௸ॣć

ດʗˋʩʇଋɬ1X Glycoprotein Denaturing Buffer

Ī0.5% SDSĂ40 mM DTTī໘Ɂ100ŎĂ10ယ࠰

ဿ౯ȯȵɥă1X G5 Reaction Buffer໘ɁĂ37Ŏ

ɁɼˋʅʽʱĜʏʿˋȳɥă

༂Ʌɢɥ࿤ߤ౯ݛć65ŎĂ10ယ࠰

࿥മ૚ᄞć

10X Glycoprotein Denaturing Buffer 10X G5 Reaction Buffer

ယાᆲć

Endo Hć29,000 Da Endo Hfć70,000 Da

࿚ଋࠣᆅćʀʅʕʈ˂ʋʏʘĜʔɞʗˋʩʇယ

ސߤ౯ɈʋˋʗʷʥĜʏʿˋȦॳ୸ȯɦɄȞȭ ɂɬߊ໻ȱɀȞɥă

ʾʣʛʠฤࡹć1 unitɉĂ೔ཱܾᅗܕ10 ̂lෂĂ

37ŎĂ1૪࠰ɁĂဿ౯ȯȵȹ10 ̂gɈRNase B

ȥɣ95%ڬ௫ɈດɬதࢦȳɥȹɛɅྼᅟɄਊ೧

ᆲɂȱɀฤࡹȯɦɥăĪ10 NEB units = 1 IUB

milliunitī ༏๱ć

Endo H༏๱ć 500,000 units/ml Endo Hf ༏๱ć1,000,000 units/ml

ʹʺćEndo HȤɢɍEndo Hf Ɉਊ೧ߤ౯ɉSDS Ɉ܆ࣗɬିȫɄȞă

ྥဿ౯Ɉດʗˋʩʇଋɬංʈ˂ʋʏ˃ݛȳɥȹ ɛɅɉĂɼˋʅʽʱĜʏʿˋ૪࠰ɬෳȩȱĂݿ ௰ᆲɈਊ೧ɬ๒ݡȳɥȭɂȦྼᅟɂɄɥ௲ਗȦ Ȝɥă

ઞ৽ဦॷćwww.nebj.jpɬઞ௎

n

X

Asn

Y

( )

EndoHȤɢɍEndo Hf ɉĂ਒ʶˋʦĜʑ৭ഢ

Īn = 2 - 150Ăx = ĪManī1-2 Ăy = HīȤɢɍʧ

ɼʮ˂ʛʡ৭ഢĪn = 2Ăx and/or y = AcNeu-Gal-GlcNAcīɈəɬಜඣȳɥ

■_{ດʗˋʩʇଋȥɣ਒ʶˋʦĜʑळ}_N_य़

ਗळດ੍ɬதࢦ

PNGase F

#P0704S 15,000 units . . . .¥ 23,500 #P0704L 75,000 units . . . .¥ 94,000 PNGase F (Glycerol Free)

#P0705S 15,000 units . . . .¥ 23,500 #P0705L 75,000 units . . . .¥ 94,000

ತცćPeptide-N-Glycosidase FɉPNGase Fɂ

ȱɀɜඪɣɦĂNय़ਗळດʗˋʩʇଋȥɣ਒ʶˋ

ʦĜʑĂʧɼʮ˂ʛʡळʂ˂ʌດĂထਗळʂ˂ʌ

ດɈێྈ໘തɈGlcNAc રࡊɂɺʑʩˁʆˋર

ࡊɈ࠰ɬಜඣȳɥɺʷʘĜʔɁȜɥăHPLCယ಑

ᅙɅʈ˂ʓ˅Ĝ˃ʭ˂ĜɈਊ೧ɜอࢷȱɀȞɥă

ᄻᅰćFlavobacterium meningosepticumȥɣ ౼౿ă

ཱܾ௸ॣć

ດʗˋʩʇଋɬ1X Glycoprotein Denaturing Buffer

Ī0.5% SDSĂ40 mM DTTī໘ɁĂ100ŎɁ10ယ࠰ ဿ౯ȯȵɥă1X G7 Reaction BufferɅ1% NP-40ɬ

๒ݡȱɀĂ37ŎɁɼˋʅʽʱĜʏʿˋȳɥă

࿥മ૚ᄞć

10X Glycoprotein Denaturing Buffer 10X G 7 Reaction Buffer

10% NP-40

ယાᆲć36,000 Da

࿚ଋࠣᆅćʀʅʕʈ˂ʋʏʘĜʔɞʀˋʡʈ˂

ʋʏʘĜʔF1ĂF2ɘȹɉF3ߤ౯ɈʋˋʗʷʥĜ

ʏʿˋȦॳ୸ȯɦɄȞȭɂɬߊ໻ȱɀȞɥăɘ ȹĂʯ˅ʞɺĜʔɈʋˋʗʷʥĜʏʿˋȦॳ୸ ȯɦɄȞȭɂɜߊ໻ȱɀȞɥă

ʾʣʛʠฤࡹć1 unitɉĂ೔ཱܾᅎܕ10 ̂lෂĂ

37ŎĂ1૪ ࠰ Ʌ Ȥ Ȟ ɀĂ ဿ ౯ ȯ ȵ ȹ10 ̂gɈ

RNaseBȥɣ95%ڬ௫ɈດɬதࢦȳɥȹɛɅྼ

ᅟɄਊ೧ᆲɂȱɀฤࡹȯɦɥăĪ65 NEB units =

1 IUB milliunitī

༏๱ć500,000 units/mlĪ#P0704, #P0705ī ʹʺćPNGase Fߤ౯ɉSDSɅɢȽɀ೫ޮȯɦ

ɥȹɛĂཱܾᅗܕෂɅɉNP-40ɬྼȴ๒ݡȳɥă

ྥဿ౯Ɉດʗˋʩʇଋɬංʈ˂ʋʏ˃ݛȳɥȹ ɛɅɉĂɼˋʅʽʱĜʏʿˋ૪࠰ɬෳȩȱĂݿ ௰ᆲɈਊ೧ɬ๒ݡȳɥȭɂȦྼᅟɂɄɥ௲ਗȦ Ȝɥă

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PNGase FɉĂʈ˂ʋʲʯʙʡ/ດʗˋʩʇଋȥ

ɣĂɕɖȳɓɀɈʗɼʯɈNय़ਗळດ੍ɬݡ౉

ယސȳɥăĮx = HɘȹɉດĪ੍īį

■_{ດʗˋʩʇଋȥɣ}_N_{य़ਗळດ੍ɬංʈ}

˂ʋʏ˃ݛ

■_{௢੧Ʉ৭ഢູڽ౯Ʌ࠲ȱɀɉ}₂₂₇_ʲĜ

ʐɬઞ௎

࠲ᇦ౿࿚ć

RNase B (control substrate)

#P7817S 250 µg . . .¥ 11,000

Endoglycosidase Reaction Buffer Pack 10X G5 and G7 Reaction Buffers, 10X Glycoprotein Denaturing Buffer, 10% NP-40 (1 ml of each)

#B0701S 4 x 1.0 m . . . .¥ 2,700

糖鎖生物学＆

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O

_-Glycosidase

#P0733S 2,000,000 units . .¥ 21,000 #P0733L 10,000,000 units . .¥ 84,000

ತცćO-Glycosidaseɉ

Endo-˷-N-Acetylgalactosaminidaseɂ ȱ ɀ ɜ

ඪɣɦɀȤɤĂດʗˋʩʇଋȥɣOय़ਗळʐʍʛ

ʃˁɼʡɬCore1ȤɢɍCore3Ɂಜඣȳɥă

ᄻᅰćEnterococcus faecalisȥɣʇ˅Ĝʣˋʈ ȱɀ൥෬ࣳɁཡএă

࿥മ૚ᄞć

10X Glycoprotein Denaturing Buffer 10X G7 Reaction Buffer

10% NP-40

྘ߤ౯ć53,000,000 units/mg

࿚ଋࠣᆅćʀʅʕʈ˂ʋʏʘĜʔȤɢɍʯ˅ʞ

ɺĜʔɈʋˋʗʷʥĜʏʿˋȦॳ୸ȯɦɄȞȭ ɂɬߊ໻ȱɀȞɥă

37ŎĂ1૪ ࠰ Ʌ Ȥ Ȟ ɀĂ5 mgɈneuraminidase

ယސ੟əFetuinĪྥဿ౯īȥɣ0.68 nmolɈOय़ ਗ౯ʐʍʛʃˁɼʡɬதࢦȳɥȹɛɅྼᅟɄਊ

೧ᆲɂȱɀฤࡹȯɦɥăĪ1 unitɈO-glycosidase

ɂPNGase Fɉȷɦȸɦຑʺ˃ɈOय़ਗळʐʍʛ

ʃˁɼʡɂNय़ਗळʂ˂ʌດɬಜඣɁȧɥī

༏๱ć40,000,000 units/ml

ʹʺćʏɺ˃બȦശ੭ȳɥ௲ਗɅɉதࢦɬȱɀ

ȥɣO-GlycosidaseɁಜඣɬ਄Ƞă

࠲ᇦ౿࿚ć

O-Glycosidase & Neuraminidase Bundle #E0540S 1 bundle . . . .¥ 31,000 Neuraminidase

#P0720S 2,000 units . . . .¥ 11,000 #P0720L 10,000 units . . . .¥ 44,000

β(1–3)

-α-O-Ser/Thr

β(1–3)

-α-O-Ser/Thr

■_{ດʗˋʩʇଋȥɣ}_O_{य़ਗळʐʍʛʃˁ}

ɼʡɬCore1ȤɢɍCore3Ɂಜඣ

ʐɬઞ௎

Remove-iT

™

_{PNGase F}

#P0706S 6,750 units . . . .¥ 36,500 #P0706L 33,750 units . . .¥ 146,000

ತ ცćRemove-iT PNGase FɉNय़ ਗळດ ʗˋ ʩʇଋȥɣ਒ʶˋʦĜʑĂʧɼʮ˂ʛʡळʂ˂

ʌດĂထਗळʂ˂ʌດɈێྈ໘തɈGlcNAcરࡊ

ɂɺʑʩˁʆˋરࡊɈ࠰ɬಜඣȳɥɺʷʘĜʔ ɁȜɥăRemove-iT PNGase FɅɉʅʙˋय़ਗ

ʡʹɼˋĪCBDīʗʈȦᅃਗȱɀȤɤĂཱܾᅗ

ܕȥɣࠤඍɅதࢦȦݢ༑ɁȜɥăɘȹHPLCɞଋ

ᆲယ಑Ʌ੕ไɄʈ˂ʓ˅Ĝ˃ʭ˂Ĝɂȱɀอࢷ ȱɀȞɥă

ᄻᅰćFlavobacterium meningosepticumȥɣ ౼౿ă

ཱܾ௸ॣć

ດʗˋʩʇଋɬ1X DTTĪ40 mMī໘ɁĂ55Ŏ

Ɂ10ယ࠰ဿ౯ȯȵɥă1X G7 Reaction Bufferෂ

ɅȤȞɀ37ŎɁ1૪࠰ɼˋʅʽʱĜʏʿˋȳɥă

࿥മ૚ᄞć

10X DTT

10X G 7 Reaction Buffer

࿚ଋࠣᆅćʀʅʕʈ˂ʋʏʘĜʔɞʀˋʡʈ˂

ʋʏʘĜʔF1ĂF2ɘȹɉF3ߤ౯ɈʋˋʗʷʥĜ

ʏʿˋȦॳ୸ȯɦɄȞȭɂɬߊ໻ȱɀȞɥăɘ ȹĂʯ˅ʞɺĜʔɈʋˋʗʷʥĜʏʿˋȦॳ୸ ȯɦɄȞȭɂɜߊ໻ȱɀȞɥă

37ŎĂ1૪࠰ɅȤȞɀĂDTTɁဿ౯ȯȵȹ5 ̂g

ɈRNaseBȥɣ95%ڬ௫ɈດɬதࢦȳɥȹɛɅ ྼᅟɄਊ೧ᆲɂȱɀฤࡹȯɦɥă

༏๱ć225,000 units/ml

ʹʺćSDSɂDTTɬ࠸ɚGlycoprotein Denaturing Bufferɬ઴ᅙȱɀํळโɄဿ౯௸ॣɁRNase B

ɬဿ౯ȯȵȹ௲ਗĂRemove-iT PNGase FɈৌ

ᆎɉ500,000 U/mlɘɁ৐௫ȳɥă

SDSɂDTTɬ࠸ɚGlycoprotein Denaturing Buffer

ɬ઴ᅙȱɀດʗˋʩʇଋɬဿ౯ȯȵȹ௲ਗĂཱܾ

ᅗܕෂɅྼȴNP-40ɬݡȢɥăȭɦɉ

Remove-iT PNGase FȦSDSɁ೫ޮȯɦɥȹɛɁȜɥă

ྥɼʂˋ౯ޢჶߤ౯੬ȦݝঠSDSɈ೫ޮɬ൅Ȼ

௉ȳɈȥɉယȥȽɀȞɄȞă

Remove-iT PNGase Fɬཱܾᅗܕȥɣதࢦȳɥ ௲ਗĂʅʙˋ૱౯ʫĜʒɈᆲɬഝɞȱɀʑʉĜ ˃ɺʛʯȳɥȭɂȦɁȧɥă

X

Asn

Remove-iT PNGase FɉĂʈ˂ʋʲʯʙʡ/ດ

ʗˋʩʇଋȥɣĂɕɖȳɓɀɈʗɼʯɈNय़ਗ

ळດ੍ɬݡ౉ယސȳɥăĮx = HɘȹɉດĪ੍īį

■_{ດʗˋʩʇଋȥɣ}_N_{य़ਗळດ੍ɬංʈ}

˂ʋʏ˃ݛ

ʐɬઞ௎

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Remove-iT Endo D

#P0742S 1,500 units . . . .¥ 36,500 #P0742L 7,500 units . . .¥ 146,000

ತ ცćRemove-iT Endo DɉEndoglycosidase

DɂȱɀඪɣɦɀȞɥ˂ʋˋʫʢˋʠʈ˂ʋʏ

ʘĜʔɁȜɥă૊ယȥɦɈෳȯɅ࠲ɩɣȴĂʩ

ɾʙʶˋʦĜʑळɈNय़ਗळດ੍ɬಜඣȳɥă

Remove-iT Endo DɅ ɉ ʅ ʙ ˋ य़ ਗ ʡ ʹ ɼ ˋ

ĪCBDīʗʈȦᅃਗȱɀȤɤĂཱܾᅗܕȥɣࠤඍ

ɅதࢦȦݢ༑ɁȜɥăɘȹHPLCɞଋᆲယ಑Ʌ੕

ไɄʈ˂ʓ˅Ĝ˃ʭ˂ĜɂȱɀอࢷȱɀȞɥă

ᄻ ᅰćStreptococcus pneumoniaeȥ ɣ ʠ ˁ ˋ ʉĜʠळEndo Dۆ๙ાɬʇ˅ĜʣˋʈȱɀĂ൥

෬ࣳ໘ɁCBDᅃਗʗˋʩʇଋɂȱɀཡএă

࿥മ૚ᄞć

10X DTT

10X G7 Reaction Buffer

྘ߤ౯ć25,900 units/mg

37ŎĂ1૪࠰ɁĂʈ˂ʋʏʘĜʔɁʠ˂ʷˋʈ

Īʠ˂ʶˋʦʏ˃ʋɺīȱȹ10 ̂gɈFetuinȥɣ

95%ڬ௫ɈດɬதࢦȳɥȹɛɅྼᅟɄਊ೧ᆲɂ

ȱɀฤࡹȯɦɥă

X

Asn

X = ĪHɘȹɉʂ˂ʌʍʛʃˁɼʡī

■_{ດʗˋʩʇଋɞດʲʯʙʡȥɣʩɾʙ}

ʶˋʦĜʑळNय़ਗळດ੍ɬதࢦ

■ _N-_{ʈ˂ʋʏ˃ݛငڮɈड़ฤɅ઴ᅙݢ༑}

Remove-iT Endo S

#P0741S 6,000 units . . . .¥ 36,500 #P0741L 30,000 units . . .¥ 146,000

ತცćRemove-iT Endo SɉʥɼʞɻʮIgGɈ୭

੍ɈʅʠʫʂĜʑʋɺȥɣ਒Ȟູڽ౯ɁNय़ਗळ

ດ੍ɬதࢦɁȧɥʀˋʡʈ˂ʋʏʘĜʔɁȜɥă

Remove-iT Endo SɅ ɉ ʅ ʙ ˋ य़ ਗ ʡ ʹ ɼ ˋ

ĪCBDīʗʈȦᅃਗȱɀȤɤĂཱܾᅗܕȥɣࠤඍ

ɅதࢦȦݢ༑ɁȜɥăɘȹHPLCɞଋᆲယ಑Ʌ੕

ไɄʈ˂ʓ˅Ĝ˃ʭ˂ĜɂȱɀอࢷȱɀȞɥă

ᄻᅰćStreptococcus pyogenesȥɣʇ˅Ĝʣˋ

ʈȯɦĂ൥෬ࣳ໘ɁCBDᅃਗʗˋʩʇଋɂȱɀ

ݿ௰ཡএă

࿥മ૚ᄞć

10X G6 Reaction Buffer

྘ߤ౯ć636,000 units/mg

37ŎĂ1૪࠰ɁĂ5 ̂gɈIgGȥɣ95%ڬ௫Ɉດ ɬதࢦȳɥȹɛɅྼᅟɄਊ೧ᆲɂȱɀฤࡹȯɦ

ɥăංʈ˂ʋʏ˃ݛɉSDS-PAGEɁߊ໻ȯɦɥă

_(1–6)

`(1–4) `(1–4) `(1–4)

_(1–3) _(2–6) `(1–4) `(1–2)

_(2–6) `(1–4) `(1–2)

_(1–6)

X

[ ]

( )

■ ʥɼʞɻʮ_IgGȥɣ_Nय़ਗळດ੍ɬதࢦ

■_N-_{ʈ˂ʋʏ˃ݛငڮɈड़ฤɅ੕ไ}

■_{௢੧Ʉ৭ഢູڽ౯Ʌ࠲ȱɀɉ}₂₂₈_ʲĜ

ʐɬઞ௎

Remove-iT

ਊ೧Ɉ࠲ᇦ౿࿚

Chitin Magnetic Beads

#E8036S 5 ml . . . ¥ 18,000

#E8036L 25 ml . . . ¥ 72,000

6-Tube Magnetic Separation Rack #S1506S 6 tubes . . . .¥ 34,000

12-Tube Magnetic Separation Rack #S1509S 12 tubes . . . .¥ 55,000

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Protein Deglycosylation Mix

#P6039S 20 reactions . . . .¥ 81,000 ತ ცćProtein Deglycosylation Mixɉ ං ʈ ˂ ʋ ʏ˃ݛɅྼᅟɄਊ೧ɂʨʛʭɹĜɬ࠸ɚਊ೧ʃ

ʇʞ˃ɁȜɥăNय़ਗळດ੍ȺȫɁɉɄȩĂඍ஋

ɄOय़ਗळດ੍ɞOय़ਗळထਗດ੍ɜංʈ˂ʋʏ

˃ݛȳɥȭɂȦɁȧɥăႨʅʛʠɅɉංʈ˂ʋ

ʏ˃ݛཱܾ20ޑယɈ૚ᄞȦ࠸ɘɦɀȤɤĂ੕൥

Ɂ2 mgɈດʗˋʩʇଋɈංʈ˂ʋʏ˃ݛɬ਄Ʉ

ȠȭɂȦɁȧɥă

࿥മ૚ᄞć

Deglycosylation Enzyme Mix 10X Glycoprotein Denaturing Buffer 10% NP-40 Buffer

10X G7 Reaction Buffer Fetuin Control

Deglycosylation Enzyme Mix:

20 μl PNGase F Glycerol Free: 500,000 units/ml 20 μl O-Glycosidase: 40,000,000 units/ml 20 μl Neuraminidase: 50,000 units/ml 20 μl β1-4 Galactosidase: 8,000 units/ml 20 μl β-N-Acetylglucosaminidase: 4,000 units/ml

1 2 3 4 5

O-Glycosidase

β1-4 Galactosidase

β-N-Acetylglucosaminidase

Untreated Fetuin Deglycosylated Fetuin

Neuraminidase

PNGase F

ɾʏFetuinɈਊ೧โංʈ˂ʋʏ˃ݛć

ဿ౯௸ॣݚɅȤȞɀ100 ̂gɈɾʏFetuinɈංʈ

˂ʋʏ˃ݛɬ਄Ƚȹă25 ̂lɈཱܾᅗܕɬ10 -

20% SDS-PAGEʊ˃Ʌɺʯˁɼȱȹă˄Ĝˋ1ć

Protein LadderĪNEB #P7703īĂ˄Ĝˋ2ć25 ̂g

ྥဿ౯Fetuinʋˋʠ˅Ĝ˃Ă˄Ĝˋ3ć25 ̂gဿ౯

Fetuinʋˋʠ˅Ĝ˃Ă˄Ĝˋ4ć25 ̂gංʈ˂ʋ ʏ˃ݛဿ౯FetuinĂLane5ć5 ̂l Deglycosylation Enzyme Mix

■_{ʓʛʠɺʛʯȦᅎڹȥȾ఻ബ}

■_N_य़ਗळ_{/ O}_{य़ਗळɈດ੍ɬৌᆎโɅ}

ංʈ˂ʋʏ˃ݛɁȧɥਊ೧ʃʇʞ˃

■_ྥဿ౯_/_{ဿ౯ɈȞȴɦɈ௸ॣɁɜ઴ᅙ}

ݢ༑

■_{ਊ೧โཱܾɅɢɤດ੍ɒɈഹ஥ȦɄȞ}

ȹɛĂංʈ˂ʋʏ˃ݛȱȹດ੍Ɉယ಑ Ʌ੕ไ

Fetuin

#P6042S 500 µg . . . .¥ 13,500 ತცćFetuinɉʏɺ˃ݛȯɦȹNय़ਗळ / Oय़ਗ ळດ੍ɬ࠸ɚດʗˋʩʇଋɁȜɥă߂ʀˋʡʈ ˂ʋʏʘĜʔɈʵʐʞɻʮʋˋʠ˅Ĝ˃ɂȱɀ ઴ᅙɁȧɥă

ᄻᅰćɾʏ൙૥ॠ౸

ယાᆲć64 kDa

༏๱ć10 mg/ml

ʹʺć500 ̂gɉᄝ20ޑယɅ୥ယɄᆲɁȜɥă

ʈ˂ʋʏ˃ݛȦ࿤ࣧێɁȜɥȹɛĂSDS-PAGE

௫Ɂɉ2ႨɈʨˋʡɂȱɀॳ୸ȯɦɥă

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α

-

N

_{-Acetylgalactosaminidase}

#P0734S 3,000 units . . . .¥ 22,500 #P0734L 15,000 units . . . .¥ 90,000

ತ ცć˷-N-AcetylgalactosaminidaseɉĂ ʗ ˋ

ʩʇଋɅय़ਗȱɀȞɥʂ˂ʌດɂNय़ਗळດ੍ȥ

ɣ˷य़ਗळD-N-ɺʓʙ˃ʄˁʇʠʍʷˋરࡊɈݡ

౉ယސɬఌ༲ȳɥ਒Ȟູڽ౯ɬᄵȳɥʀʅʕʈ ˂ʋʏʘĜʔɁȜɥă

ᄻ ᅰćChryseobacterium meningosepticumȥ ɣʇ˅Ĝʣˋʈȯɦ൥෬ࣳ໘Ɂཡএă

ཱܾ௸ॣć1X G7 Reaction BufferĂ100 ̂g/ml

BSA ɬ๒ݡă37ŎɁɼˋʅʽʱĜʏʿˋă

࿥മ૚ᄞć10X G7 Reaction BufferĂ100X BSA

྘ߤ౯ć~ 20,000 units/mg

ʾʣʛʠฤࡹć1 unitɉĂ37ŎĂ1૪࠰ɅȤȞɀĂ

೔ཱܾᅎܕ10 ̂lෂĂ1 nmolɈĪGalNAc ˷1-3ī ĪFuc˷1-2īGal˸1-4Glc-AMCȥɣ95%ڬ௫Ɉ

˷-D-N-ɺʓʙ˃ʄˁʇʠʍʷˋჂ඘ɬಜඣȳɥ

ȹɛɅྼᅟɄਊ೧ᆲɂȱɀฤࡹȯɦɥă

α(1–2)

α(1–3)

α(1–4)

α(1–6) R

β

-

N

_{-Acetylglucosaminidase}

#P0732S 100 units . . . .¥ 21,000 #P0732L 500 units . . . .¥ 84,000

ತცć˸-N-Acetylglucosaminidase ɉĂʂ˂ʌ

ດȥɣჂ඘Ɉྥ࠮ই௻ൔɈ˸-N-ɺʓʙ˃ʈ˃ʋ

ʍʷˋરࡊɈݡ౉ယސɬఌ༲ȳɥ਒Ȟູڽ౯ɬ ᄵȳɥʀʅʕʈ˂ʋʏʘĜʔɁȜɥă

ᄻᅰćXanthomonas manihotisȥɣʇ˅Ĝʣˋ ʈȯɦĂ൥෬ࣳ໘Ɂཡএă

ཱܾ௸ॣć 1X G1 Reaction BufferĂ100 ̂g/ml

BSAɬ๒ݡă37ŎɁɼˋʅʽʱĜʏʿˋă

ʾʣʛʠฤࡹć1 unitɉĂ೔ཱܾᅗܕ10 ̂lෂĂ37ŎĂ

1૪࠰ɅȤȞɀĂ1 nmolɈGlcNAc˸

1-4GlcNAcb1-4GlcNAc-AMCȥɣ95%ڬ௫ɈჂ඘ɬಜඣȱɀྥ

࠮ইळ˸-N-ɺʓʙ˃ʈ˃ʋʍʷˋરࡊɬಜඣȳ

ɥȹɛɅྼᅟɄਊ೧ᆲɂȱɀฤࡹȯɦɥă

β(1–2)

β(1–3)

β(1–4)

β(1–6) R

■_{௢੧Ʉ৭ഢູڽ౯Ʌ࠲ȱɀɉ}₂₂₆_ʲĜ

ʐɬઞ௎

β

-

N

_{-Acetylhexosaminidase}

f

#P0721S 500 units . . . .¥ 11,000 #P0721L 2,500 units . . . .¥ 44,000

ತ ცć˸-N -AcetylhexosaminidasefɉĂ˸-N- Acetylhexosaminidaseɂʶ˃ʠĜʑय़ਗʗˋʩ ʇଋɈ˂ʋˋʫʢˋʠʗˋʩʇଋᅃਗൌɁȜɤĂ ᄘ౺ळɂວێɈߤ౯ɬᄵȳɥăႨਊ೧ɉʂ˂ʌ

ດȥɣɈ˸-D-N-ɺʓʙ˃ʄˁʇʠʍʷˋჂ඘ɂ

ʂ˂ʌດɈʈ˃ʋʍʷˋરࡊɈݡ౉ယސɬఌ༲ ȳɥă

ᄻᅰćStreptomyces plicatusȥɣʇ˅Ĝʣˋʈ ȯɦĂ൥෬ࣳ໘Ɂݿ௰ཡএă

ཱܾ௸ॣć1X G2 Reaction Bufferă37ŎɁɼˋ ʅʽʱĜʏʿˋ

࿥മ૚ᄞć10X G2 Reaction Buffer

37ŎĂ1૪࠰ɅȤȞɀĂ1 nmolɈGalNAc˸1-4Gal

˸1-4Glc-AMCȥɣ95%ڬ௫Ɉ˸-D-N-ɺʓʙ˃

ʄˁʇʠʍʷˋჂ඘ɬಜඣȳɥȹɛɅྼᅟɄਊ ೧ᆲɂȱɀฤࡹȯɦɥă

β(1–3)

β(1–4)

β(1–6) R

β(1–4) R

■_{෸੍௻ࡊଋɅɈəߤ౯ɬ૲ȳ}

α

1-2 Fucosidase

#P0724S 1,000 units . . . .¥ 22,500 #P0724L 5,000 units . . . .¥ 90,000

ತცć˷1-2 FucosidaseɉĂʂ˂ʌດȥɣɈྥ ယࡎ˷1-2य़ਗळL-FucopyranosylરࡊɈݡ౉ယ

ސɬఌ༲ȳɥູڽโɄʀʅʕʈ˂ʋʏʘĜʔɁ Ȝɥăࡊଋɉယࡎ৭ഢɬ૩ȹɄȞ෸੍௻ࡊଋɅ ঔฤȯɦɥă

ယાᆲć~ 70,000 Da

37ŎĂ1૪࠰ɅȤȞɀĂ1 nmolɈFuc˷1-2Gal˸

1-4Glc-AMCȥɣ95%ڬ௫Ɉ˷-L-fucoseɬಜඣ ȳɥȹɛɅྼᅟɄਊ೧ᆲɂȱɀฤࡹȯɦɥă

α(1–2) R

■_{෸੍௻ࡊଋɅɈəߤ౯ɬ૲ȳ}

ʐɬઞ௎

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α

1-3,6 Galactosidase

#P0731S 100 units . . . .¥ 21,000 #P0731L 500 units . . . .¥ 84,000

ತცć˷1-3,6 Galactosidase ɉĂʂ˂ʌດȥɣ ˷1-3,6य़ਗळD-ʄˁʇʠʬˁʦʏ˃રࡊɈݡ౉ယ ސɬఌ༲ȳɥ਒Ȟູڽ౯ɬᄵȳɥʀʅʕʈ˂ʋʏ ʘĜʔɁȜɥă

૖ไཱܾ૪࠰Ȥɢɍਊ೧༏๱ɉࡊଋɅܾȲɀ੕ไ ݛȳɥྼᅟȦȜɥă

࿥മ૚ᄞć 10X G6 Reaction BufferĂ100X BSA

37ŎĂ1 ૪࠰ɅȤȞɀĂ1 nmol ɈGal˷1-3Gal˸

1-4Gal-AMCȥɣ95%ڬ௫Ɉ˷-D-ʄˁʇʠĜʑ

Ⴢ඘ɬಜඣȳɥȹɛɅྼᅟɄਊ೧ᆲɂȱɀฤࡹ ȯɦɥă

α(1–3)

α(1–6) R

ʐɬઞ௎

β

1-3 Galactosidase

#P0726S 500 units . . . .¥ 22,500 #P0726L 2,500 units . . . .¥ 90,000

ತცć˸1-3 GalactosidaseɉĂʂ˂ʌດȥɣ˸

1-3य़ਗळD-ʄˁʇʠʬˁʦʏ˃રࡊɈݡ౉ယސ

ɬఌ༲ȳɥ਒Ȟູڽ౯ɬᄵȳɥʀʅʕʈ˂ʋʏ

ʘĜʔɁȜɥăɘȹྥ௵ɅඵȞബ๱Ɂ˸1-6य़ਗ

ळD-ʄˁʇʠʬˁʦʏ˃રࡊɈݡ౉ယސɜఌ༲ȳ

ɥă˸1-3य़ਗɈݡ౉ယސബ๱ɉĂ˸1-6य़ਗɈ

ݡ౉ယސɢɤ100༰ڬ௫ബȩĂɘȹ˸1-4य़ਗɈ

ݡ౉ယސɢɤ500༰ڬ௫ബȞă

37ŎĂ1૪࠰ɅȤȞɀĂ1 nmolɈGal˸1-3GlcNAc

˸1-3Gal˸1-4Glc-AMCȥɣ95%ڬ௫Ɉ˸-D-ʄ

ˁʇʠĜʑჂ඘ɬಜඣȳɥȹɛɅྼᅟɄਊ೧ᆲ ɂȱɀฤࡹȯɦɥă

༏๱ć10,000 units/m

β(1–3)

β(1–6)

β(1–4) R < <<

ʐɬઞ௎

β

1-4 Galactosidase

#P0730S 400 units . . . .¥ 21,000 #P0730L 2,000 units . . . .¥ 84,000

ತცć˸1-4 GalactosidaseɉĂʂ˂ʌດȥɣ˸

1-4य़ਗळD-ʄˁʇʠʬˁʦʏ˃રࡊɈݡ౉ယސ

ɬఌ༲ȳɥ਒Ȟູڽ౯ɬᄵȳɥʀʅʕʈ˂ʋʏ ʘĜʔɁȜɥă

ᄻᅰćBacteroides fragilisȥɣʇ˅Ĝʣˋʈȯ ɦĂ൥෬ࣳ໘Ɂཡএă

ཱܾ௸ॣć1X G4 Reaction Bufferă37ŎɁɼˋ ʅʽʱĜʏʿˋă

ཱܾ૪࠰Ȥɢɍਊ೧༏๱ɉࡊଋɅܾȲɀ੕ไݛ ȳɥྼᅟȦȜɥă

๒࿥૚ᄞć10X G4 Reaction Buffer

37ŎĂ1૪࠰ɅȤȞɀĂ1 nmolɈGal˸1-4GlcNAc

˸1-3Gal˸1-4Glc-AMCȥɣ95%ڬ௫Ɉ˸-D-ʄ

ˁʇʠĜʑჂ඘ɬಜඣȳɥȹɛɅྼᅟɄਊ೧ᆲ ɂȱɀฤࡹȯɦɥă

β(1–4) R

ʐɬઞ௎

α

1-2,3 Mannosidase

#P0729S 640 units . . . . .¥ 22,500 #P0729L 3,200 units . . . . .¥ 90,000

ತცć˷1-2,3MannosidaseɉĂʂ˂ʌດȥɣ˷

1-2य़ਗळȤɢɍ˷1-3य़ਗळD-ʶˋʦʬˁʦʏ˃

રࡊɈݡ౉ယސɬఌ༲ȳɥ਒Ȟູڽ౯ɬᄵȳɥʀ ʅʕʈ˂ʋʏʘĜʔɁȜɥă

37ŎĂ1૪࠰ɅȤȞɀĂ1nmolɈMan˷1-3Man

˸1-4GlcNAc-AMCȥ ɣ95%ڬ ௫ Ɉ˷-D-ʶ ˋ

ʦĜʑჂ඘ɬಜඣȳɥȹɛɅྼᅟɄਊ೧ᆲɂȱ ɀฤࡹȯɦɥă

α(1–2)

α(1–3) R

ʐɬઞ௎

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α

1-6 Mannosidase

#P0727S 800 units . . . .¥ 22,500 #P0727L 4,000 units . . . .¥ 90,000

ತცć˷1-6 MannosidaseɉĂʂ˂ʌດȥɣɈ

ྥယࡎ˷1-6य़ਗळD-ʶˋʦʬˁʦʏ˃રࡊɬத

ࢦȳɥ਒Ȟູڽ౯ɬᄵȳɥʀʅʕʈ˂ʋʏʘĜ

ʔɁȜɥă˷1-2,3 Mannosidaseɂဩᅙȳɥȭ

ɂɅɢȽɀĂယࡎ৭ഢɬᄵȳɥດ੍ȥɣ˷1-6

ʶˋʦĜʑરࡊɬಜඣȳɥȭɂȦɁȧɥă

BSA ɬ๒ݡă37ŎɁɼˋʅʽʱĜʏʿˋă

ʾʣʛʠฤࡹć1 unitɉĂ37ŎĂ1૪࠰ɅȤȞɀĂ

೔ ཱ ܾ ᅗ ܕ10 ̂lෂĂ1 nmolɈMan˷1-6 Man

˷1-6 Man-AMCȥɣ95%ڬ௫Ɉ˷-D-ʶˋʦĜʑ

Ⴢ඘ɬಜඣȳɥȹɛɅྼᅟɄਊ೧ᆲɂȱɀฤࡹ ȯɦɥă

ʹʺćp-ʣʠ˅ʭɿʣ˃-˷-D-ʶˋʦʬˁʦʏʡ

ɉႨਊ೧ɈࡊଋɁɉɄȞă

α(1–6) R

ʐɬઞ௎

Neuraminidase

#P0720S 2,000 units . . . .¥ 11,000 #P0720L 10,000 units . . . .¥ 44,000

ತცćNeuraminidaseɉɺʓʙ˃ʦɼˁʷˋબ ʪʡˁĜʔĪʏɺ˂ʘĜʔīɈێཾშɁȜɥă ȭɈNeuraminidaseɉດʗˋʩʇଋɂʂ˂ʌດ ȥ ɣ˷2-3Ă˷2-6ɂ˷2-8य़ ਗ ळN-ɺ ʓ ʙ ˃ ʦ ɼˁʷˋબરࡊɈݡ౉ယސɬఌ༲ȳɥă

ᄻ ᅰćClostridium perfringensȥ ɣ ʇ ˅ Ĝ ʣ ˋ

ʈȯɦĂNEBɁ൥෬ࣳ໘Ɂݿ௰ཡএă

ཱܾ௸ॣć1X G1 Reaction Bufferă37ŎɁɼˋ ʅʽʱĜʏʿˋă

࿥മ૚ᄞć10X G1 Reaction Buffer

ʾʣʛʠฤࡹć1 unitɉĂ೔ཱܾᅗܕ10 ̂lෂĂ5ယ

࠰Ă37ŎɅȤȞɀĂ1 nmolɈNeu5Ac˷2-3Gal˸

1-3GlcNAc˸1-3Gal˸1-4Glc-AMC ȥɣჂ඘Ɉ ˷-Neu5AcɈ95œڬ௫ɬಜඣȳɥȹɛɅྼᅟɄ ਊ೧ᆲɂȱɀฤࡹȯɦɥă

ʹʺćႨਊ೧ɉ˷2-8य़ਗɂ྘ߏȱɀĂ˷2-3य़

ਗɂ˷2-6य़ਗɬᄭಪโɅಜඣȳɥă

࠲ᇦ౿࿚ć

O-Glycosidase & Neuraminidase Bundle #E0540S 1 bundless . . . .¥ 31,000

α(2–3)

α(2–6)

α(2–8) R <<

■_{pH 4.5 - 8.5}_{ɅȤȞɀߤ౯ɬ૲ȳ}

■௢੧Ʉ৭ഢູڽ౯Ʌ࠲ȱɀɉ₂₂₈ʲĜ

ʐɬઞ௎

α

2-3 Neuraminidase

#P0728S 2,500 units . . . .¥ 11,000 #P0728L 12,500 units . . . .¥ 44,000

ತ ცć˷2-3 Neuraminidaseɉ ʂ ˂ ʌ ດ ȥ ɣ˷

2-3य़ਗळǪ-ɺʓʙ˃ʦɼˁʷˋબરࡊɈݡ౉

ယސɬఌ༲ȳɥ਒Ȟູڽ౯ɬᄵȳɥʀʅʕʈ˂

ʋʏʗĜʔɁȜɥă˷2-6य़ਗळN-ɺʓʙ˃ʦɼ

ˁʷˋબરࡊɅൎȳɥಜඣബ๱ɉඵȞăȭɈਊ

೧ɉ˷2-6ʏɺ˃य़ਗɢɤ˷2-3ʏɺ˃य़ਗɅൎ

ȱɀູڽ౯Ȧ260༰਒ȩĂ˷2-8ʏɺ˃य़ਗɅൎ

ȱɀɉɕɂɭɃູڽ౯ɬ૲ȯɄȞă

ᄻᅰćSalmonella typhimurium LT2ȥɣʇ˅Ĝ ʣˋʈȯɦĂ൥෬ࣳɁݿ௰ཡএă

྘ߤ౯ć~ 11,300,000 units/mg

1૪ ࠰Ă37Ŏ Ʌ Ȥ Ȟ ɀĂ1 nmolɈNeu5Ac˷

2-3Gal˸1-3GlcNAc˸1-3Gal˸1-4Glc-AMCȥ ɣ˷-Neu5Acɬ95%ڬ௫ಜඣȳɥȹɛɅྼᅟɄ ਊ೧ᆲɂȱɀฤࡹȯɦɥă

ʹʺć˷2-3य़ਗɬೊ൰โɅݡ౉ယސȳɥɅɉĂ

100 ̂g/ml BSAɬ ๒ ݡ ȱ ȹ1X G4 Reaction Bufferɬ઴ȽɀႨਊ೧ɬ10༰ࡏଫȱȹɜɈɬᅙ Ȟɥȭɂɬైயȳɥă

α(2–3)

α(2–6)

α(2–8) R <<

<<

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Deteiled Characterization of Several Glycosidase Enzyme

˸1-3 Galactosidase

Ⴈਊ೧ɉ଱Ʌʂ˂ʌດෂɈ˸1-3य़ਗɬಜඣȳɥȦĂɺʦʶĜ৭ഢɁȜɥGlcNAcĪ˸1-6īરࡊȦ

ᆿಞȳɥ௲ਗɅঔɤĂਊ೧Ɉູڽ౯Ʌ੼ᅙȱĂྥ࠮ই˸1-4ʄˁʇʠĜʑɜಜඣȳɥă

GlcNAc˸1-6ɺʦʶĜȦࣷಞȱɀȞɥ௲ਗĂ౿࿚ෂɈਊ೧༏๱Ī10,000 units/mīɁɉĂూAɈ

ɢȠɅ˸1-4ʄˁʇʠĜʑय़ਗȦಜඣȯɦɥăێၫĂ16༰Ʌࡏଫȱȹਊ೧ɬ઴ᅙȱȹ௲ਗĂూBɈ

ɢȠɅ˸1-4ʄˁʇʠĜʑय़ਗɉಜඣȯɦɄȞă

β(1–4) β(1–6 )

β(1–4)

β(1–3) β(1–3)

β(1–4) β(1–6)

β(1–4)

β(1–3) β(1–3)

A. 0.1 nm/µl substrate, 10 units, 1 hr incubation B. 0.1 nm/µl substrate, 0.625 units, 1 hr incubation

˸1-4 Galactosidase

ూAĂBĂCɉȷɦȸɦ2Ă4Ă8 unitsɈ˸1-4 GalactosidaseɬᅙȞȹɂȧɈಜඣɈᅕાɁȜɥă

ཱܾɉ1X G4ɜȱȩɉ1X G6 Reaction Bufferɬ࠸ɚཱܾᅗܕ10 ̂lෂɁĂ37ŎɁ਄Ƚȹă

β(1–4) β(1–2)

β(1–4)

β(1–4) β(1–2) α(1–6)

α(1–3)

β(1–4) β(1–2) _α

(1–6)

β(1–4) β(1–2)

α(1–3)

β(1–4)

β(1–4) β(1–4)

β(1–6)

β(1–4)

α_(1–6)

β(1–4)

β(1–3)

β(1–6) β(1–4)

α(1–3)

β(1–4)

β(1–6)

β(1–4)

A. 0.1 nm/µl substrate, 2 units, 1 hr incubation B. 0.1 nm/µl substrate, 4 units, 1 hr incubation C. 0.1 nm/µl substrate, 8 units, 1 hr incubation

˸-N -Acetylglucosaminidase

ూAĂBĂCĂDɉȳɓɀ1X G1 Reaction Bufferɂ1X BASɬ࠸ɚཱܾᅗܕ10 ̂lෂɁĂ4 unitsɈ

ਊ೧ɬᅙȞɀ37ŎɁཱܾɬ਄ȽȹɂȧɈಜඣɈᅕાɁȜɥăູɅBĂCĂDɅ࠲ȱɀɉĂཱܾ೐Ʌ

8 unitsɈ˸1-4 GalactosidaseɁ೐ஐᆅɬ਄Ƚȹă

β(1–4) β_(1–6)

β(1–4)

β(1–3) β(1–3) β(1–4)

α_(1–6

) β(1–6)

β(1–2)

β(1–4)

α(1–3)

β(1–2)

β(1–4)

β(1–4) β(1–2)

β(1–4) β(1–4)

α_(1–6

)

β(1–4)

α(1–3)

β(1–2) β(1–4) β(1–2) _α

(1–6)

β(1–4) _β(1–2)

α(1–3)

β(1–4)

β(1–4) β(1–4)

B. 0.1 nm/µl substrate, 4 hr incubation

A. 0.1 nm/µl substrate, 4 hr incubation C. 0.1 nm/µl substrate, 18 hr incubation D. 0.1 nm/µl substrate, 24 hr incubation

˷1-2,3 Mannosidasee

ూAĂBĂCĂDɉȳɓɀ1X G6 Reaction Bufferɂ1X BASɬ࠸ɚཱܾᅗܕ10 ̂lෂɁĂ32 unitsɈ

ਊ೧ɬᅙȞɀ37ŎɁཱܾɬ਄ȽȹɂȧɈಜඣɈᅕાɁȜɥă

β(1–4)

α(1–6)

α(1–3)

β(1–4) α_(1–6) α(1–3

)

β(1–4)

α_(1–6

)

α(1–3)

α(1–6)

α(1–3) β(1–4)

α_(1–6)

α(1–3 )

α(1–3

) α_(1–6) α_(1–6)

α(1–2) α(1–2)

α(1–2) α(1–2) α

(1–3)

β(1–4)

A. 0.1 nm/µl substrate, 1 hr incubation B. 0.1 nm/µl substrate, 1 hr incubation C. 0.1 nm/µl substrate, 18 hr incubation D. 0.1 nm/µl substrate, 18 hr incubation

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˷1-6 Mannosidase

ݚూɈཱܾɅȤȞɀĂ1X G6 Reaction Bufferɂ1X BASɬ࠸ɚཱܾᅗܕ10 ̂lෂɁĂ32 unitsɈ˷

1-2, 3 MannosidaseĪNEB #P0729īɂ40 unitsɈ˷1-6 MannosidaseĂȤɢɍȷɦȸɦɈࡊଋ

ɬݡȢɀ37ŎɁɼˋʅʽʱĜʏʿˋɬ਄ȽȹăూEɅࡥȯɦɀȞɥࡊଋɉࠊ೔ɅɉಜඣȯɦɄȥȽ

ȹăȭɈȭɂȥɣEɈɢȠɄ৭ഢȦࡊଋෂɅ࠸ɘɦɥ௲ਗĂ˷1-6 MannosidaseɁɉಜඣȯɦɄȞ

ȭɂȦᅅ೻ȯɦɥă

ʹʺć˷1-6 Mannosidaseɬඍ຾Ɂ઴ᅙȱȹ௲ਗĂ෸੍௻ࡊଋȺȫɬಜඣȳɥȭɂɅ෉ڷă˷

1-2, 3 Mannosidaseɂဩᅙȱȹ௲ਗĂယࡎ৭ഢɬᄵȳɥດ੍ෂɈ˷1-6ʶˋʦĜʑરࡊɬಜඣȳ ɥȭɂȦɁȧɥă α_(1–6) α(1–3) α_(1–6) α(1–3) α_(1–6) α(1–3 ) β(1–4) α_(1–6 ) α(1–3) α_(1–6 ) α(1–3) β(1–4) α_(1–6) α(1–3 ) α(1–2) α(1–3) α_(1–6) α_(1–6) α(1–2) α(1–2) α(1–2) α(1–2) α

(1–3)

β(1–4)

α(1–3)

α_(1–6)

α(1–2)

α(1–2) α (1–3)

β(1–4)

A. 0.1 nm/µl substrate, 4 hr incubation

B. 0.1 nm/µl substrate, 4 hr incubation

C. 0.1 nm/µl substrate, 18 hr incubation

D. 0.05 nm/µl substrate, 18 hr incubation

E. 0.045 nm/µl substrate, 18 hr incubation

˷1-3,6 Galactosidase

ۦూAɉ24 unitsɈ˷1-3, 6 GalactosidaseȤ ɢɍ100 unitsɈNeuraminidaseɬᅙȞɀཱܾ

ɬ਄ȽȹষĂ65ŎɁ10ယ࠰Ɉ༂଄ߤɬ਄ȞĂ

റ Ȟ ɀ16 unitsɈ˸1-4 GalactosidaseɁ2૪ ࠰ཱܾɬ਄ȽȹɂȧɈಜඣɈᅕાɁȜɥă

ۦ ూBɉ4 unitsɈ˷1-3, 6 Galactosidaseɬ ᅙȞɀཱܾɬ਄ȽȹɂȧɈಜඣɈᅕાɁȜ

ɥăཱܾɉ1X G6 Reaction Bufferɂ1X BAS

ɬ࠸ɚ20 ̂lɈཱܾᅗܕෂɁ਄Ƚȹă

α(1–3) α(1–3)

α(1–2)

X

α(1–2)

B. 0.1 nm/µl 1 hour incubation (Does not cut if a branch fucose is present.) A. 0.245 nm/µl substrate, 18 hr incubation

α(2–6)

or β(1–4) β(1–2)

α(1–3)

α(2–6) β(1–4) β(1–2)

α

(1–3)

α(1–3) β(1–4) _β

(1–4 )

β(1–6)

β(1–4) β(1–4)

α

(1–6)

α(1–3) β(1–4)

α(1–3)

˷1-2 Fucosidase

ݚూAĂBĂDɉ1X G4 Reaction BufferĂCɉ1X G6 Reaction BufferෂɅɀཱܾɬ਄Ƚȹăཱܾ

ɉȞȴɦɜĂ1X BSAɬ࠸ɚཱܾᅗܕ10 ̂lෂɁĂ37ŎɅɀ਄Ƚȹă೔ɀɈृɅ20 unitsɈ˷1-2

Fucosidaseɬ ๒ ݡ ȱ ȹă ູ ɅCɅ ɉ20 unitsɈ˷-N-AcetylgalactosaminidaseĪNEB #P0734ī

ɜ๒ݡȱȹăCɁɉယࡎ৭ഢɬᄵȳɥ˷1-2ʭʋĜʑɉĂ˷1-2 Fucosidaseඍ຾ɁɉಜඣȯɦɄȥȽ

ȹȦĂ˷-N-Acetylgalactosaminidaseɂဩᅙȱȹ௲ਗɉಜඣȯɦȹă

β(1–4) α(1–2)

α(1–2) β(1–3)

α

(1–4)

β(1–4) β(1–2)

α(1–2) β(1–3)

β_(1–6) β(1–4) β(1–3) α (1–4) β(1–4) α (1–3)

α(1–3) β(1–3)

α

(1–2)

β(1–4) β(1–2)

A. 0.1 nm/µl substrate, 1 hr incubation B. 0.1 nm/µl substrate, 1 hr incubation C. 0.05 nm/µl substrate, 1 hr incubation D. 0.1 nm/µl substrate, 1 hr incubation

PNGase F, Remove-iT PNGase F

PNGase FɉĂ੕ɜ໘തɅڮ඲ȳɥGlcNAcર

ࡊȦ˷1-3ʭʋĜʑરࡊɅय़ਗȱɀȞɥ௲ਗĂ

ດʗˋʩʇଋȥɣNय़ਗळດ੍ɬಜඣȳɥȭ

ɂȦɁȧɄȞĪ1īăɄȤĂȭȠȱȹୌఄʩʗĜˋ

ɉఆဘɜȱȩɉێငɈਹ්ᄻᅰɈດʗˋʩʇ ଋɅ଱Ʌॽɣɦɥă

(1) Tretter, V. et al. (1991)

Eur. J. Biochem.

199,

647-652.

β(1–4) α_(1–6 ) α(1–3 ) β(1–4) Asn x x α (1–6) β(1–4) α_(1–6) α(1–3) β(1–4) Asn x x α (1–3) β(1–4) α_(1–6) α(1–3 ) β(1–4) Asn x x

X

α (1–3) β(1–4) α_(1–6) α(1–3) β(1–4) Asn x x α (1–6)

_X

A. PNGase F can cleave when an α1-6 Fucose is on the core GlcNAc

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Remove-iT Endo S

Remove-iT Endo SɈࡊଋɉউდɅɉດʗˋ

ʩʇଋɁɄȩɂɜɢȞăȾɘɤۦూɈĨXĩɉ

ʗ ˋ ʩ ʇ ଋĂ ʲ ʯ ʙ ʡĂ ɺ ʑ ʩ ˁ ʆ ˋ ɞ ʭ

˂ĜɈດ੍ɂɄɥăRemove-iT Endo Sɉယ

ࡎȳɥN-ɺʓʙ˃ʈ˃ʋʍʷˋɈᄵჟɅ࠲ɩ

ɣȴߤ౯ɬ૲ȱĂɘȹʋɺ˷1-6ʭʋʏ˃ݛɈ

ᄵჟɅ࠲ɩɣȴߤ౯ɬ૲ȳă3ႨယࡎȜɥȞɉ

4ႨယࡎɈʏɺ˃ݛດ੍ȜɥȞɉɺʏɺ˅ດ੍

ɉRemove-iT Endo SɈࡊଋɁɉɄȞă

__(1–6)

`(1–4) `(1–4) `(1–4)

_(1–3) _(2–6) `(1–4) `(1–2)

_(2–6) `(1–4) `(1–2)

_(1–6)

X

[ ]

( )

O -Glycosidase

ĪEndo-˷-N-Acetylgalactosaminidaseī

ూAɂBɉ1X G7 reaction bufferɬ࠸ɚ೔ཱܾ ᅎᆲ100 ̂lෂĂ40,000 unitsɈO-Glycosidase

ɂ100 unitsɈNeuraminidaseɁಜඣɬ਄Ƚȹ

૪ɈᅕાɁȜɥă37ŎɁɼˋʅʽʱĜʏʿˋ

ɬ਄ȞĂOय़ਗळດ੍ɉMorgan-Elsonɺʛʓ

ɼɁॳ୸ɬ਄Ƚȹă

β(1–3)

-α-O-Ser/Thr

A. 5 mg substrate, 1 hr incubation, Core 1 Cleavage

β(1–3)

-α-O-Ser/Thr

B. 5 mg substrate, 1 hr incubation, Core 3 Cleavage

Neuraminidase

Neuraminidaseɉʂ˂ʌດɈᆲɞယࡎ৭ഢɅ ɢȽɀယࡎȳɥʏɺ˃બɬಜඣȱɄȞȥĂȜ

ɥȞɉಜඣৌᆎȦฝȞĪ1īăۦూɈʂ˂ʌດ

ɉFetuinෂɅശ੭ȳɥɜɈɁȜɤĂಜඣȯɦ ɄȞယࡎ৭ഢɈێᇉɁȜɥă

(1) Iwamori, M., Ohta, Y., Uchida, Y., Tsukada, Y., (1997) Glycoconj J. (1): 67-73.

(2) Green, E.D., et al. (1998) J. Biol. Chem. 263(34): 18253-18268.

_(2–3/6)

X

_(2–3/6)

Bacteroides

_{Haparinase I}

#P0735S 240 units . . . . .¥ 25,500 #P0735L 600 units . . . . .¥ 51,000

ತ ცćBacteroides Haparinase IɉBacteroides eggerthiiȥ ɣ ʇ ˅ Ĝ ʣ ˋ ʈ ȯ ɦĂ ʰ ʩ ˂ ˋ ˂

ɺĜʔIɂɜঘɊɦɥਊ೧ɁȜɤĂʰʩ˂ˋȤɢ

ɍʰʩˁˋᆘબෂɈ਒๱ᆘબݛʡʹɼˋɅൎȱ ɀ੼ᅙȳɥăཱܾɅɢɤၼሆȱɀȞɄȞɾ˅ˋ

બɬ࠸ɚʂ˂ʌດદဘɬદ౺ȱĂUV࢓৉๱ोɁ

232 nmɁॳ୸Ɂȧɥă

ᄻᅰćBacteroides eggerthiiȥɣʇ˅Ĝʣˋʈ ȯɦĂ൥෬ࣳ໘Ɂཡএă

ཱܾ௸ॣć೔ཱܾᅗܕ100 ̂lෂĂ10 ̂gɈʰʩ˂ˋ ࡊଋɂ10 ̂lɈBacteroides Heparinase Reaction Bufferă30ŎɁɼˋʅʽʱĜʏʿˋă

࿥മ૚ᄞć

10X Bacteroides Heparinase Reaction Buffer

࿚ ଋ ࠣ ᆅćʀ ʅ ʕ ʈ ˂ ʋ ʏ ʘ Ĝ ʔĂ ʍ ˃ ʭ ɹ ʗ Ĝ ʔĂ ɾ ˅ ʣ ʘ Ĝ ʔ Ȥ ɢ ɍ ʯ ˅ ʞ ɺ Ĝ ʔ Ɉ ʋˋʗʷʥĜʏʿˋȦॳ୸ȯɦɄȞȭɂɬߊ໻ ȱɀȞɥă

30ŎĂpH 7.0ɅȤȞɀĂʮʗ༈ႸᄻᅰɈʰʩ˂ˋ

ȥɣ1.0 ̂molɈၼሆȱɀȞɄȞʂ˂ʌດɬᄿᆋ

ȳɥȹɛɅྼᅟɄਊ೧ᆲɂȱɀฤࡹȯɦɥă

ʈ˃ʇ˅ˋબȤɢɍɼʒ˅ˋબɁɜᆯȞ ਊ೧ູڽ౯ɈȹɛɈड़ฤᅟۚɉಘ૦Ɂ૲ ȯɦɀȞɥ

■_{ʰʩ˂ˋȤɢɍʰʩˁˋᆘબɈʈ˂ʋ}

ʍʷʦດɬယސ

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Bacteroides

_{Haparinase II}

#P0736S 80 units . . . .¥ 38,500 #P0736L 200 units . . . .¥ 77,000

ತცćBacteroides Haparinase IIɉBacteroides eggerthiiȥ ɣ ʇ ˅ Ĝ ʣ ˋ ʈ ȯ ɦĂ ʰ ʩ ˂ ˋ ˂

ɺĜʔIIɂɜঘɊɦɥਊ೧ɁȜɤĂʰʩ˂ˋȤɢ

ཱܾ௸ॣć೔ཱܾᅗܕ100 ̂lෂĂ10 ̂gɈʰʩ ˂ ˋ ࡊ ଋ ɂ10 ̂lɈBacteroides Heparinase Reaction Bufferă30ŎɁɼˋʅʽʱĜʏʿˋă

࿥മ૚ᄞć10X Bacteroides Heparinase Reaction Buffer

30ŎĂpH 7.0ɅȤȞɀĂʮʗ༈ႸᄻᅰɈʰʩ˂ˋ

ȥɣ1.0 ̂molɈၼሆȱɀȞɄȞʂ˂ʌດɬᄿᆋ

ȳɥȹɛɅྼᅟɄਊ೧ᆲɂȱɀฤࡹȯɦɥă

ʍʷʦດɬယސ

Bacteroides

_{Haparinase III}

#P0737S 14 units . . . .¥ 52,000 #P0737L 35 units . . .¥ 104,000

ತცćBacteroides Haparinase IIIɉBacteroides eggerthiiȥ ɣ ʇ ˅ Ĝ ʣ ˋ ʈ ȯ ɦĂ ʰ ʩ ˂ ˋ ˂

ɺĜʔIIɂɜঘɊɦɥਊ೧ɁȜɤĂʰʩ˂ˋȤɢ

ཱܾ௸ॣć೔ཱܾᅗܕ100 ̂lෂĂ10 ̂gɈʰʩ ˂ ˋ ࡊ ଋĪ1 mg/mlī ɂ10 ̂lɈBacteroides

Heparinase Reaction Bufferă30Ŏ Ɂɼ ˋʅʽ ʱĜʏʿˋă

࿥മ૚ᄞć10X Bacteroides Heparinase Reaction Buffer

ʾʣʛʠฤࡹć೔ཱܾᅗܕ100 ̂lෂĂ30ŎɅȤ

Ȟ ɀĂ2༰ ࡏ ଫ ȱ ȹBacteroides Heparinase III

ɂ1 mg/mlɈ ʮ ʗ ༈ Ⴘ ᄻ ᅰ Ɉ ʰ ʩ ˂ ˋ ᆘ બ ɬ ɼˋʅʽʱĜʠăၼሆȱɀȞɄȞʂ˂ʌດɈᄿ

ᆋɬ˂ɺ˃ʗɼʸUV࢓৉๱ोɬᅙȞɀ232 nm

Ɂॳ୸ă

༏๱ć700 units/ml

ʍʷʦດɬယސ

Heparin Hexasaccharides, MS Standard

Heparin Hexasaccharide MS Standard 6 #P0738S 40 nmol . . . .¥ 76,500 Heparin Hexasaccharide MS Standard 7 #P0739S 40 nmol . . . .¥ 76,500

ತცćHeparin Hexasaccharide MS Standard 6

ɉݚూɈ༯ᇙɂ৭ഢɬᄵȳɥଋᆲယ಑ᅙɈʑʗˋ

ʘĜʡɁȜɥăBacteroides Heparinase Iɬ઴ᅙ

ȱɀʮʗ༈ႸᄻᅰɈʰʩ˂ˋɬಜඣȱȹદဘȥ

ɣ౼౿ȯɦɀȤɤĂ6ȾɈᆘબࡊɬᄵȳɥă

Heparin Hexasaccharide MS Standard 7ɉ ݚ ూɈ༯ᇙɂ৭ഢɬᄵȳɥଋᆲယ಑ᅙɈʑʗˋ

ʘĜʡɁȜɥăBacteroides Heparinase Iɬ઴ᅙ

ȱɀʮʗ༈ႸᄻᅰɈʰʩ˂ˋɬಜඣȱȹદဘȥ

ɣ౼౿ȯɦɀȤɤĂ7ȾɈᆘબࡊɬᄵȳɥă

ᄻ ᅰćBacteroides Heparinase Iɬ ઴ ᅙ ȱ ɀ ʮ ʗ༈ႸᄻᅰɈʰʩ˂ˋɬಜඣȱȹદဘȥɣ౼౿

ݛߔૺćC38H59N3O49S6 (NEB #P0738),

C38H59N3O52S7 (NEB #P0739)

ယાᆲć1,533.05 (NEB #P0738), 1,613.01 (NEB #P0739)

ଋᆲ๝ݷ྘ćMS Standard 6 [M-1H]1-_{1532.04; [M-2H]}

2-765.52; [M-3H]

510.01; [M-4H]

382.25. MS Standard 7 [M-1H]1-_{1612.00; [M-2H]}2-_{805.50; [M-3H]}3-_536.66;

[M-4H]

402.24.

ʺ˃࢓৉बౘć3800 L· mol-1

· cm-1

࿚ଋࠣᆅćଋᆲယ಑ɅȤȫɥHexasaccharideɂ

ʦɼʒɈ྘ᆎȦ95%ڬ௫ɁȜɥȭɂɬߊ໻ȱɀ

Ȟɥă

Heparin Hexasaccharide MS Standard 6.

Heparin Hexasaccharide MS Standard 7.

∆UA2S - GlcNS6S - UA - GlcNAc6S - IdoA - GlcNS6S

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Ph.D

™

_{Phage Display Peptide Library Kits}

Ph.D.-7 Kit

10 Panning Experiments

#E8100S . . . .¥ 68,500 Ph.D.-12 Kit

#E8110S . . . .¥ 68,500 Ph.D.-C7C Kit

#E8120S . . . .¥ 81,000

+

ʯ ˄ Ĝ ʠ Ʌ ʋ Ĝ ʞ ɻ ˋ ʈ ȯ ɦ ȹ ʗĜʊʛʠယાɅ ൎȱɀĂڽɄɥʲ ʯʙʡ༯ᇙɬอ૲ ȳɥʭɹĜʐˁɼ ʮˁ˂ɬ๒ݡȳɥ

ʗĜʊʛʠယાɅ ൎ ȳ ɥ ࡕ ඪ Ɉ ˂ ʄˋʡɬݿ௰Ʌ๒ ݡ ȳ ɥĂ ɘ ȹ ɉ pHɬ ݚ Ȭ ɥ ȭ ɂ ɅɢȽɀູڽโɅ य़ਗȱȹʭɹĜʐ ɬᅗ୸ȳɥ ᅗ୸ȱȹʭɹĜ

ʐɬഝဍȳɥă ȭɦɣɈݿาɬ 3ޑटɤ၄ȳ

3ޑटɤ၄ȱȹষĂ ߂ʇ˅Ĝˋɬඍᆋ ȱɀʏĜʉˋʑɬ ȤȭɄȠ ჎य़ਗɈʭɹĜʐ ɬಸȞᆔȳ

ూ1ćʩʣˋʈଌঅɈޱᅟ

ತცćႨʅʛʠɉĂ໸ڷɈʗĜʊʛʠယાĪৣൌĂ

ਊ೧Ă੧ၲ࿍ჶିᅎൌɄɃīɅൎȳɥ˂ʄˋʡယ ાɬʑʇ˂ĜʣˋʈȳɥȹɛɈʅʛʠɁȜɥă ʭɹĜʐʟɻʑʯ˄ɼĆˁɼʮˁ˂ĜɁɉĂʲ ʯʙʡɞʗˋʩʇଋȦʭɹĜʐᆙાɈެതɅཡ এȯɦɀȤɤĂȷɦɣɬʋĜʡȳɥۆ๙ાɉ໘ തɅശ੭ȱɀȞɥȹɛĂʗˋʩʇଋĪ࿍এृī

ɂȷɦɬʋĜʡȳɥDNAĪۆ๙ळīɬဘᆅโɄ

ວێယા௫Ɂय़ɍȾȫɥȭɂȦݢ༑ɁȜɥăȯ ɣɅĂʗĜʊʛʠယાɒɈय़ਗపሆ౯ɅࡊȿȞ

ȹʩʣˋʈɂঘɊɦɥin vitroʑʇ˂Ĝʣˋʈၭ

ɅɢȽɀ఻ബɅʭɹĜʐȦယކȯɦɥăూ1Ʌɉ

ʩʣˋʈၫၭɈޱᆒȦऻ੪ȯɦɀȞɥăɘȴĂ ʗĜʊʛʠယાɁʋĜʞɻˋʈȯɦȹʯ˄Ĝʠ ĪɘȹɉʫĜʒī௫ɁʭɹĜʐʲʯʙʡĆˁɼʮ ˁ ˂ Ĝ ɬ ɼ ˋ ʅ ʽ ʱ Ĝ ʏ ʿ ˋ ȱĂ ჎ य़ ਗ Ɉ ʭ ɹ Ĝ ʐ ɬ ಸ Ȟ ᆔ ȱ ȹ ষĂ ູ ڽ โ Ʌ य़ ਗ ȱ ȹ ʭ ɹ Ĝ ʐ Ɉ ə ɬ ᅗ ୸ ȳ ɥă ȯ ɣ Ʌ ᅗ ୸ ȯ ɦ ȹ ʭɹĜʐɬഝဍȱĂວᅕɅय़ਗĆಸ௺Ćഝဍɬट ɤ၄ȳȭɂɅɢȽɀĂʗĜʊʛʠယાɅय़ਗȳɥ ˂ʄˋʡယાɬอ૲ȳɥʭɹĜʐɬ༏୴Ɂȧɥă

3 ġ 4ޑटɤ၄ȱȹষɅĂDNAʏĜʉˋʏˋʈɂ

ELISAၭɅɢɤ˂ʄˋʡယાɈೊฤɬȤȭɄȠă

New England BiolabsɁɉĂ3ହᇆɈˁˋʘʸʲ ʯʙʡĆˁɼʮˁ˂ĜɈ഼Ăʃʑʗʸˁɼʮˁ

˂Ĝ੼౿ᅙɈʇ˅ĜʣˋʈʱʇʗĜĪM13KEī

ɬอࢷȱɀȞɥăˁˋʘʸʲʯʙʡĆˁɼʮˁ

˂ĜɂȱɀĂ෸੍௻ʰʯʗʲʯʙʡĪPh.D.-7īĂ

ʡʟʃʲʯʙʡĪPh.D.-12īĂʐʑ˃ʭɻʡݫ࣌

ȯɦȹʰʯʗʲʯʙʡĪPh.D.-C7Cīˁɼʮˁ

˂ĜɬᅙڷȱɀȞɥăPh.D.-C7Cˁɼʮˁ˂Ĝ

Ɉʲʯʙʡඣ၀ɈᆤჂ඘ɅɉĂʏʠʏˋરࡊȦ ࿥ݡȯɦɀȞɥăȭɈʏʠʏˋરࡊൎɉʭɹĜ ʐɺʛʓˋʮ˂ෂɅબݛȱɀʐʑ˃ʭɻʡय़ਗ ɬव౰ȳɥȭɂɅɢɤĂʭɹĜʐ࿍ჶɅȤȞɀ ʲʯʙʡȦ˃Ĝʯɂȱɀอ૲ȯɦɥăȳɓɀɈ

ˁɼʮˁ˂ĜɅ࠸ɘɦɥʇ˅Ĝˋɉ20ݍହᇆɬ

෰ȢɥăɘȹĂ3ହᇆɈˁɼʮˁ˂ĜȳɓɀɈʲ

ʯʙʡ༯ᇙɉĂʶɼʢĜʋĜʠʗˋʩʇଋpIIIɈ

NჂ඘ɅᅃਗཡএȯɦĂʭɹĜʐᆙાຎȹɤ5ʋ

ʬĜɈʲʯʙʡȦอ૲ȯɦɥăɘȹĂPh.D.-7ˁ

ɼʮˁ˂ĜȤɢɍPh.D.-12ˁɼʮˁ˂ĜɁɉĂ

ʲʯʙʡ-pIIIᅃਗʗˋʩʇଋɈˁˋʘʸʲʯʙʡ

༯ᇙȦNჂ඘ĪʭɹĜʐȥɣެതīɅڮ඲ȳɥȦĂ

Ph.D.-C7Cˁɼʮˁ˂ĜɁɉNჂ඘ɉAla-CysɁ ȜɤĂȭɦɅറȞɀˁˋʘʸʲʯʙʡ༯ᇙȦڮ ඲ȳɥăɄȤĂȳɓɀɈˁɼʮˁ˂ĜɉĂอ૲

ȯɦɥʲʯʙʡɂpIIIɈ࠰Ʌ඗Ȟ˂ˋʃĜ༯ᇙ

ĪGly-Gly-Gly-SerīȦ࠸ɘɦɥă

Ph.D.ˁɼʮˁ˂ĜɉĂʀʬʠĜʯʶʛʬˋʈ

Īూ2īĂʗˋʩʇଋ-ʗˋʩʇଋय़ਗȤɢɍਊ೧

೫ޮ੬ɈວฤĂGroELĂHIVĂ཰ຩൌ࿍ჶɞழယ

ાʭ˃ʂ˅ʭʁɺɅൎȳɥʲʯʙʡ˂ʄˋʡɈ ʑʇ˂Ĝʣˋʈɞᄞ੬ާཡɄɃഽȩɈɺʯ˂ ʉĜʏʿˋɁ઴ᅙȯɦɀȞɥăɘȹĂ౼౿ȯɦ ȹିᅎൌȤɢɍɼˋʗʇʠɄ੧ၲɬʗĜʊʛʠ ယાɂȱȹʩʣˋʈɅɢȽɀĂିᅎൌɅൎȱɀ ౺ဘߤ౯ɬᄵȳɥ˂ʄˋʡɜວฤȯɦɀȞɥă ȯɣɅູฤɈ੧ၲळɬʗĜʊʛʠɅȳɥʲʯʙ ʡɜin vitroʩʣˋʈɅɢȽɀඍᆋȯɦĂ੧ၲູ ڽโۆ๙ાຩ໴ɅܾᅙȯɦɀȞɥăʃʫၲાɞ

੧ࣳɅൎȳɥ˂ʄˋʡĂᇉȢɊin vitroɂin vivo

Ɂඖᢴຽ೧ɬູڽโɅ೫ޮȳɥʲʯʙʡɄɃɜĂ Ⴈʏʑʞʸɬ઴ᅙȱɀວฤȯɦɀȞɥăɘȹĂ ʭɹĜʐɬ຦ဘɅ෉໴ȱɀĂ࠲ᇦȳɥࡉࠋɬେ ୠȱĂ߂೨ఉȥɣʭɹĜʐɬඍᆋȳɥȭɂɅɢ

ɥin vivoʩʣˋʈɅɢȽɀĂ೨ఉູڽโʲʯʙ

ʡɜඍᆋȯɦɀȞɥă

ઞ৽ဦॷćwww.nebj.jpɬઞ௎ă

ˁɼʓˋʑ/ູࢮ/ჲಗૢਐć379-382ʲĜʐɬઞ௎ ඍ࿚ྀ༸ć

Ph.D.-7 Library 50 Panning Experiments

#E8102L . . . .¥ 275,000 Ph.D.-12 Library

#E8111L . . . .¥ 275,000 Ph.D.-C7C Library

#E8121L . . . .¥ 315,000

Ph.D.ʅʛʠ໘ᅎć

ĝ Libraryć ʩ ʣ ˋ ʈ ଌ অ10ޑ ယĂ ᄝ

109ହᇆɈˁˋʘʸʲʯʙʡɬอ૲ȳ

ɥ ʭ ɹ Ĝ ʐ ʟ ɻ ʑ ʯ ˄ ɼ ˁ ɼ ʮ ˁ ˂Ĝ

ĝ 28 gIII Sequencing PrimerĪ100 pmolī

ĝ 96 gIII Sequencing PrimerĪ100 pmolī

ĝ E. coli strain ER2738

ĝ StreptavidinĪʋˋʠ˅Ĝ˃ʗĜʊʛʠī ȤɢɍBiotinĪᅗᆋܕī

ĝ Protocols

ʏĜʉˋʑʯˁɼʶĜ༯ᇙ

Īඍ࿚ྀ༸ɉȱɀȞɘȵɭīĈ

-28 glll Sequencing Primer (22-mer)

5´d(GTATGGGATTTTGCTAAACAAC)3´

-96 glll Sequencing Primer (20-mer)

5´d(CCCTCATAGTTAGCGTAACG)3´

࠲ᇦ౿࿚ć

Anti-M13 pIII Monoclonal Antibody

Anti-M13 pIII Monoclonal AntibodyĪ ʶ ɾ ʑ

ɺɼʕʗɼʯIgG2aīɉĂM13ʋĜʠʗˋʩʇ

ଋIIIɈCჂ඘ത཰ယĪ259 - 406રࡊīɁჲܖȯ

ɦȹBALB/c ʶɾʑȥɣɈʶɾʑਬౕ଺੧ၲɂ

᪷੧ၲɈᅃਗɅɢȽɀ౺ȲȹA23ʧɼʮ˂ʡĜ

ʶɅᄻᅰȳɥă

ʹʺćɼˋʗʇʠɈM13ʭɹĜʐȦɺʇʓʑɁ

ȧɄȞᆴۊɅʀʬʠĜʯȦȜɥȹɛĂႨৣൌɉ

ʭɹĜʐɬৣঈɂȱȹELISAɁ઴ᅙȳɥȭɂɬ

ైயȱɄȞă

pMAL-pIII Vector

ႨʱʇʗĜɉ

M13 pIII

˂ĜʘĜ༯ᇙɬᄵȱɀ

ȤɤĂ

Ph.D. Phage Display Peptide Library

Ɂೊ်ȯɦȹ༯ᇙɬ

MBP

ᅃਗʗˋʩʇଋɂ

ȱɀཡএĆ౼౿ȳɥȭɂȦݢ༑ɁȜɥă

#N8101S 10 µg . . . ¥ 17,500

糖鎖生物学＆

プ

ロ

テ

イ

ン

ツ

ー

ル

(16)

β-endorphin:

Y G G F M T S E K Q T P …

1st round sequences:

Y G W I S P P L H L P T

Y Q P D N P S R Q I A N

Y W P A H I R A V P M I

R L D D I K N T L A F S

S S D V Y S L Y P F I M

E F F P H P M L H N S R

D N W P Y R P S F S L S

S H N T Y S A P R P S A

S L L H Y A S S L S L M

F N Q N A E P F S S R P

H P R Q L L H H P L S P

2nd round sequences:

Y G G F L I G L Q D A S

Y G G F H Y K E T G A L

Y Q P D N P S R Q I A N

V Y C Y I N Q S M I G N

H H D T E Y R T T Q L S

N L K F P T N P K A M W

L P N L T W A L M P R A

D N W P Y R P S F S L S

S H N T Y S A P R P S A

S L L H Y A S S L S L M

V T M N T K T P G P M P

3rd round sequences:

Y G G F M T T P S H V P

Y G G F I S Q T Q H Y S

Y G G F G N S L V M P V

Y G G F S M P F L P A L

Y G A F D V T T G V T S

Y G V F N P H Y L P S L

A P S T D K Q A T M P L

A S V A V S S R Q D A A

ూ2ćPh.D.-12 ˁɼʮˁ˂ĜɅɢɥৣ˸-ʀˋʡ˃ʭɻˋʺʦʇ˅Ĝʢ˃ৣൌɈʀʬʠĜʯʶʛʬˋʈ

10 nMɈৣ˸-ʀˋʡ˃ʭɻˋৣൌ3-E7ɅൎȱɀPh.D.-12ˁɼʮˁ˂ĜɬʩʣˋʈȱȹষĂৣൌ-ʭɹĜʐထਗ ൌɬProtein A-ɺʄ˅ĜʑĪ1ޑᄆɂ3ޑᄆīĂɘȹɉProtein G-ɺʄ˅ĜʑĪ2ޑᄆīɅɺʭɻʣʞɻĜय़ਗȯ

ȵȹăȭɦɣɈय़ਗȱȹʭɹĜʐɉ0.2 M Glycine-HClĪpH 2.2īɁᅗ୸ȱȹă߂ޑɁຶɣɦȹ༯ᇙɈ໘Ă੕

஑Ɉ12રࡊȦ࿍૲ȯɦɀȞɥăɘȹĂ˸-ʀˋʡ˃ʭɻˋ༯ᇙȦ੕௫ငɅࡥ੪ȯɦɀȤɤĂȭɈഊວ༯ᇙȦڱɘ

ɦɀ࿍૲ȯɦɀȞɥăय़ݪɂȱɀĂȭɈৣൌɈʀʬʠĜʯɉ˸-ʀˋʡ˃ʭɻˋɈ੕஑Ɉ7રࡊɁȜɥȭɂȦယ

ȥɥăɘȹ˸-ʀˋʡ˃ʭɻˋɈ੕஑Ɉ4રࡊĪYGGFīȦৣঈৣൌय़ਗɅ൥ȧȩࡍᅇȱɀȞɥȦĂູɅ3ྈᄆɈ

ɺʷʦરࡊɉ഼Ʌ඲ȧࠓɩɤȠɥȭɂȦ૲੄ȯɦȹăȯɣɅĂڱɘɦɀ࿍૲ȯɦɀȞɥ။ശ༯ᇙɉĂNჂ඘ʙ

˅ʏˋɈʭ˂Ĝ˷-ɺʷʦࡊȦʀʬʠĜʯɈێငɁȜɥȭɂɬ૲ȱɀȞɥă

M13KE Phage

#N0316S 0.04 ml . . . .¥ 23,500 ತცćM13KE Phage ɉࠐಹ౯ʭɹĜʐɁȜɤĂ

Ph.D.ʇ˅ĜʣˋʈʱʇʗĜɁȜɥM13KEɅᄻ ᅰ ȳ ɥă ȭ Ɉ ʱ ʇ ʗ Ĝ ʭ ɹ Ĝ ʐ ɉĂ ʭ ɹ Ĝ ʐ

ELISAɘȹɉʭɹĜʐʑʠʛʇɈʗɼʗĜപฤɈ

ʋˋʠ˅Ĝ˃ɂȱɀ઴ᅙɁȧɥăထ౿ळM13KE

ȦPh.D.Peptide Display Cloning SystemĪNEB #E8101īɅ࿥മȯɦɀȤɤĂʇ˅ĜʣˋʈɅ઴ ᅙȳɥȭɂɜɁȧɥă

ᄻᅰćM13KEɬࠐಹȯȵȹ൥෬ࣳER2738߰ȥ ɣ࿈ஈၫၭɁယᆋ

Ph.D. Peptide Display Cloning System

Ph.D. Peptide Display Cloning System 20 µg M13KE glll Cloning Vector 16 µg Extension Primer

#E8101S . . . .¥ 25,000

ತ ცćPh.D. Peptide Display Cloning System

ɉĂʃʑʗʸʲʯʙʡĆˁɼʮˁ˂ĜɬʋĜʠ ʗˋʩʇଋᅃਗൌɂȱɀʨʇʞ˂ʂʭɹĜʐ

M13Ɉ࿍ჶ௫Ʌอ૲ȯȵɥȹɛɈʏʑʞʸɁȜ

ɥăอ૲ȯɦɥʲʯʙʡĪ࿍এृīɂȷɦɬʋĜ

ʡȳɥDNAĪۆ๙ळīɬဘᆅโɄວێယા௫Ɂ

य़ɍȾȫɥȭɂȦݢ༑ɁȜɥȹɛĂʩʣˋʈၭ ɅɢɥᅕęɄʗĜʊʛʠယાɅൎȳɥʲʯʙʡ ˂ʄˋʡɈೊ൰Ȧݢ༑ɁȜɥă࿥മɈʟɻʑʯ

˄ɼʱʇʗĜM13KEɉM13ᄾຩൌɁȜɤĂpIII

ɈNჂ ඘ Ɉ ʇ ˅ Ĝ ʣ ˋ ʈ င ڮ Ȧ ޜ ᆯ ȯ ɦĂ ߂

ʭɹĜʐຎȹɤ5ʋʬĜɈʲʯʙʡȦอ૲ȯɦ

ɥăʭɹĜʐʷʡɁɉɄȩʭɹĜʐʱʇʗĜɬ ઴ Ƞ ȭ ɂ Ʌ ɢ ɤĂ ৣ ౺ ဘ ଋ Ɉ ೊ ൰ ɞ ʰ ˃ ʩ Ĝ ʭɹĜʐɈ਼ਗࠐಹɈྼᅟ౯ȦɄȞȹɛĂഝဍ